M. Seymen, A. Dursun, D. Yavuz, E. S. Kurtar, Önder Türkmen
{"title":"Identification of water stress-tolerant edible pumpkin seed (Cucurbita pepo) genotypes using seed yield-based tolerance indices","authors":"M. Seymen, A. Dursun, D. Yavuz, E. S. Kurtar, Önder Türkmen","doi":"10.24326/asphc.2023.4424","DOIUrl":"https://doi.org/10.24326/asphc.2023.4424","url":null,"abstract":"Pumpkin is usually cultivated in arid and semiarid regions, and the lack of water stress-tolerant cultivars is a major limiting factor. Therefore, this study was carried out to identify superior water stress-tolerant genotypes. For this purpose, 44 inbred lines with superior agronomic traits were selected from the gene pool. In addition, two hybrids (G1-Mert Bey F1 and G2-Sena Hanim F1) and two landraces (G3-Hatun Tırnağı and G4-Cercevelik) with high commercial value were used as commercial cultivars. The water stress indices were calculated from seed yields from the pumpkin genotypes grown in irrigated and water stress conditions in 2017 and 2018. The stress tolerance index (STI) determines tolerant and superior genotypes. From the principal component and cluster analyses’ findings, G9, G40, G32, G36, G5, G11, G22, G30, G37, and G13 showed the highest water stress tolerance among the inbred lines. During future breeding experiments, these inbred lines may have significant potential for developing novel water stress-tolerant cultivars for pumpkin cultivation in semiarid regions.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45175465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Susceptibility of grapevine cultivars to Eutypa lata in Serbia","authors":"Sanja Živković, Tanja Predrag Vasić, Jordan Paun Marković, Darko Radovan Jevremović","doi":"10.24326/asphc.2023.4489","DOIUrl":"https://doi.org/10.24326/asphc.2023.4489","url":null,"abstract":"The species Eutypa lata is one of the most critical phytopathogenic fungi that presents a threat to vineyards by causing significant economic damage. This grapevine disease is hazardous because there is no adequate chemical protection, so after its progression, there is a decrease in yield and dieback of parts or whole infected shoots. There are also no grapevine cultivars resistant to this disease. For these reasons, in this paper, testing of the susceptibility of a pair of grapevine cultivars to this disease was carried out. The testing was performed under semicontrolled conditions and included six of the most economically essential grapevine cultivars in Serbia (Riesling italico, Cabernet Sauvignon, Cardinal, Rkaziteli, Pinot noir, and Prokupac). Testing was performed using 14 isolates of E. lata isolated in Serbia and two control isolates obtained from the INRA. For specific detection of isolates of Eutypa spp. included in the experiment, a specific pair of primers, Lata 1/Lata 2.2, was applied, and an amplicon of the expected size of approximately 385 bp was established in all isolates. In the conditions of the set experiment, of all of the domestic and foreign-tested grapevine cultivars, the local cultivar Prokupac showed greater resistance to the isolates. In contrast, the cultivars Riesling italico, Cabernet Sauvignon and Cardinal, Pinot noir showed sensitivity to the isolates of E. lata, but not to the same extent as the cultivar Rkaziteli, which proved to be susceptible.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45951923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of the yielding of plum, sour cherry, and sweet cherry trees trained to a trellis for mechanical harvesting of fruit with those trained to a leader","authors":"Z. Buler, J. Rabcewicz, P. Białkowski","doi":"10.24326/asphc.2023.4638","DOIUrl":"https://doi.org/10.24326/asphc.2023.4638","url":null,"abstract":"In 2014, plum, sour cherry and sweet cherry trees were planted in an experimental plot of the Institute of Horticulture – National Research Institute at a distance of 4.5 m between rows and, depending on the cultivar, every 1.5, 2.0 and 2.5 m in a row. The trees were trained in the shape of the letter ‘Y’, with the shoots raised at an angle of 20° or 30° to the horizontal, intended for mechanical harvesting of dessert fruit with a harvester attached to a tractor. The two tree canopy formation systems at both shoot inclination angles were compared with the standard spindle crown with a vertical leader, from which the fruit was picked by hand. After 3 years of training the trees, mechanical harvesting of dessert fruit began with a harvester designed at the Institute of Horticulture in Skierniewice. The trees formed in the shape of the letter ‘Y’ produced comparable yields, and also higher and lower yields, depending on the cultivar, in comparison with the trees trained to a spindle crown.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42893142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative leaf structural analysis of Nepeta nuda l. Plantlets, regenerated from cryopreserved shoot meristem and ex vitro-adapted plants","authors":"M. Stefanova, T. Ganeva, D. Koleva","doi":"10.24326/asphc.2023.4791","DOIUrl":"https://doi.org/10.24326/asphc.2023.4791","url":null,"abstract":"The leaf anatomy and chloroplast ultrastructure of Nepeta nuda L. plantlets regenerated in vitro from cryopreserved shoot apical meristem and in vitro-micropropagated plantlets were studied comparatively to assess whether cryoprocedure affected leaf morphogenesis. Both postcryo and in vitro plantlets failed to develop a distinguishable palisade layer, making the mesophyll appear homogeneous. Significant damage to the chloroplast envelope and substantial thylakoid ruptures were also observed. We assumed that the specific in vitro conditions more likely affected the structures than the cryotreatment itself. Light and transmission electron microscopy observations were also carried out on newly formed leaves of ex vitro-adapted plants. The examined leaf features were similar to those in the in situ plants – bifacial leaf lamina, double-layered palisade parenchyma, loosely arranged spongy parenchyma cells, and chloroplasts with intact envelope and evenly distributed throughout the stroma internal membrane system. The obtained histological and ultrastructural results revealed the retained morphogenetic potential of N. nuda plants and proved cryopreservation as a suitable method for long-term storage.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46443690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Natalia Miler, Iwona Jędrzejczyk, Paweł Trafara, Janusz Winiecki
{"title":"Effect of high-energy ionizing radiation on the DNA content and genetic variation in chrysanthemum plants regenerated from irradiated ovaries","authors":"Natalia Miler, Iwona Jędrzejczyk, Paweł Trafara, Janusz Winiecki","doi":"10.24326/asphc.2023.4912","DOIUrl":"https://doi.org/10.24326/asphc.2023.4912","url":null,"abstract":"This study aimed to evaluate the range of quantitative and qualitative genetic changes in chrysanthemum plants regenerated in vitro from ovaries irradiated with high-energy photons (5, 10, and 15 Gy) and high-energy electrons (10 Gy). The highest DNA loss (up to 11%) was observed in plants originating from ovaries treated with 10 Gy high energy. AMOVA revealed significant differences between populations of plants representing different mutagenic treatments (18% for RAPD-based and 22% for SCoT-based analysis). The incidence of genetic changes was strongly correlated with the applied type and dose of ionizing radiation. The highest genetic distances to reference plants were observed for plants regenerated from 15 Gy high-energy photon (5.8% with RAPD and 1.7% with SCoT) and 10 Gy high-energy electron-treated explants (6.0% with RAPD and 2.9% with SCoT). Considerable changes in the phenotype of mutants were not necessarily correlated with the extent of genetic alterations. Qualitative and quantitative methods of evaluating post-radiation genetic changes should be combined for reliable detection of variant plants at early developmental stages.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46751363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of different irrigation levels and varying doses of silicon applications on yield and some physiological parameters in lettuce cultivation","authors":"Yusuf Çelik","doi":"10.24326/asphc.2023.5004","DOIUrl":"https://doi.org/10.24326/asphc.2023.5004","url":null,"abstract":"The study was repeated for two years to reduce the effects of water scarcity and drought stress in lettuce cultivation. The irrigation problem was created by applying 25% (I25), 50% (I50), 75% (I75) and 100% (I100) of the evaporation amounts formed in the class-A evaporation vessel. Si0 (0 kg ha–1), Si40 (40 kg ha–1), Si80 (80 kg ha–1) and Si120 (120 kg ha–1) silicon fertilisation was applied at four different doses. Head length, head diameter, head weight, root length, and leaf fresh and dry weight were measured in harvested plants. According to the data of 2020–2021, the best results in the effect of different doses of Si applications on plant head height, head diameter, head weight and root length at different irrigation levels were recorded from I75 × Si80, I75 × Si120, I100 × Si80, I100 × Si120 applications with the same severity level. While the Si40 dose gave good results at I75 and I100 irrigation levels, its effect decreased at I25 and I50 irrigation levels. At different irrigation levels where different doses of silicon were applied, I25 irrigation had the lowest leaf chlorophyll and relative moisture content and the most severe membrane damage, while I50 irrigation had a moderate effect. Leaf chlorophyll and moisture content increased, and membrane damage decreased in I75 × 80 kg ha–1 Si, I75 × 120 kg ha–1 Si, I100 × 80 kg ha–1 Si and I100 × 120 kg ha–1 Si applications. As a result, when the effects of the applications covering two years on plant growth and yield were evaluated, the most successful irrigation levels were determined as I75, I100, and the most successful silicon doses; were determined as 80 kg ha–1 and 120 kg ha–1.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48792360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kalisz, A. Kornaś, D. Húska, Radim Zelinka, A. Sękara, R. Pokluda, Andrzej Sałata, J. Gil
{"title":"Investigating the impact of TiO2 nanoparticles on bioactive compounds in sweet pepper seedlings: a comparison of foliar and root application methods","authors":"A. Kalisz, A. Kornaś, D. Húska, Radim Zelinka, A. Sękara, R. Pokluda, Andrzej Sałata, J. Gil","doi":"10.24326/asphc.2023.5144","DOIUrl":"https://doi.org/10.24326/asphc.2023.5144","url":null,"abstract":"Engineered TiO2 nanoparticles (TiO2-NPs) are broadly produced and utilized in various consumer products. However, plant uptake of NPs may lead to disruptions in physiological and metabolic processes, particularly when the plant’s defense mechanisms are overwhelmed. In this study, sweet pepper seedlings were exposed to TiO2-NPs via foliar (2.5% suspension) and root (0.5% suspension) methods, with plants treated with distilled water serving as controls. Results showed that foliar application caused higher accumulation of Ti in leaves as compared to stems, while root exposure led to a higher increase of Ti content in stems than in leaves. Additionally, foliar application led to alterations in chemical composition of the plants, including changes in malondialdehyde (MDA), L-ascorbic acid, total phenolics content, carotenoids, in total antioxidant capacity (TAC) and antioxidant enzymes activity. Root exposure also affected enzyme activity and TAC, but also altered H2O2, MDA and glutathione content. Chlorophylls remained at stable level in the leaves of the seedlings. Overall, these studies provide important information on plant-nanoparticle interactions and the potential effects of different nanoparticle application strategies. These data indicate also that the specific nanoparticles, applied at a controlled manner, have potential to boost the plant metabolism and improve stress tolerance, which is an important factor affecting crops’ quality and productivity.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49365519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Combined effects of excess boron and salinity on the growth and ionic imbalance of lavandin (Lavandula × intermedia) plant","authors":"H. Samet, Y. Çikili, Aysun Çavuşoğlu","doi":"10.24326/asphc.2023.5007","DOIUrl":"https://doi.org/10.24326/asphc.2023.5007","url":null,"abstract":"Generally, moderate to high salinity conditions and excess boron (B) occur together as limiting factors for plant growth in the soils of arid and semiarid regions. To determine the combined effect of excessive boron, salinity stress, or both, five different levels of B (0, 0.3, 0.6, 1.2, and 1.8 mM) and 80 mM sodium chloride (NaCl) were applied to lavandin plants grown in a greenhouse. The results showed that under nonsaline conditions, biomass production in shoots and roots and photosynthetic pigment contents (chlorophyll (Chl) a, b, and Chl a + b) decreased with exceptionally high B applications compared to the control. Moreover, the bioconcentration (BCF) of B (in shoots and roots), potassium (K) concentrations (in roots), K/sodium (Na) and calcium (Ca)/Na ratios (in shoots), and Ca/B ratios (in shoots and roots) decreased for all B applications compared to the control. In contrast, all B applications caused a remarkable increase in the carotenoid (Car)/Chl ratio, B concentrations (in shoots and roots), translocation (TF) of B, and net B accumulation compared to the control. In addition, under nonsaline conditions, concentrations of K (in shoots), Ca (in shoots and roots), and K/Na and Ca/Na ratios (in roots) were significantly increased by B applications compared with the control. Under saline conditions, significant decreases in Chl b, Chl a + b, BCF of B (in shoots and roots), and Ca/B ratio (in shoots) were observed in all B applications compared to the control. However, under saline conditions, B application caused significant increases in the Car/Chl ratio, TF of B, net B accumulation, and concentrations of B (in shoots and roots), K (in shoots), Ca, and Na (in shoots and roots) compared to the control. It was concluded that although it is not seen in the growth parameters, NaCl application could effectively alleviate the harmful effects of B toxicity in lavandin plants. Under saline conditions, notable decreases in the mean B concentration in shoots could be strong evidence for this hypothesis.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41783645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of mulching on nutrient uptake and efficiency of fertilizers in mid-early cabbage production","authors":"B. Adamović, R. Čabilovski, Đ. Vojnović, Z. Ilin","doi":"10.24326/asphc.2023.4813","DOIUrl":"https://doi.org/10.24326/asphc.2023.4813","url":null,"abstract":"A three-year field trial was conducted to determine the effects of mulching with black polyethylene film and fertilizing with organic and mineral fertilizers in mid-early cabbage production. The two-factor trial was set up as a split-plot design. The main plots included unmulched and mulched treatments. The sub-plots consisted of unfertilized control and five fertilized treatments. In the first and third trial years, total cabbage yield, total head yield, and average head weight were significantly higher on black polyethylene film plots than on unmulched plots. However, in the second year, the mean air temperature during cabbage growing exceeded the long-term average, and values of the examined parameters did not differ significantly between the mulched and unmulched treatments. In all three years, amounts of removed nutrients were significantly higher in treatment with composted pig manure + mineral fertilizer. Mulching significantly increased fertilizer use efficiency in the first and third years, while the second year saw better results on unmulched plots.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48131853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Karrikins – effect on plants, interaction with other growth regulators, and potential use in horticulture","authors":"P. Marciniak, Dariusz Sochacki, K. Nowakowska","doi":"10.24326/asphc.2023.4678","DOIUrl":"https://doi.org/10.24326/asphc.2023.4678","url":null,"abstract":"Karrikins are a group of chemicals found in plant-derived smoke from burning plant material. Till now, scientists concentrated on their effect on seed germination in plants sensitive to smoke. However, there are reports on the effect of karrikins on physiology and morphology in plants which do not occur in fire areas and are not naturally treated by smoke. These chemicals positively affect the biometric parameters of the in vitro cultured plants. Recently, their effect on germination rate of pollen in several species has been confirmed. They interact with plant growth regulators enhancing their effects, for example using them together with auxins, cytokinins, gibberellins, abscisic acid or ethylene. This paper contains a review of present research on karrikins and proposes perspectives of further investigations, as well as application of these chemicals in horticultural production as a new group of plant growth regulators.","PeriodicalId":7230,"journal":{"name":"Acta Scientiarum Polonorum Hortorum Cultus","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43627863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}