J. Poirriez, D. Baccam, E. Dei‐Cas, T. Brogan, I. Landau
{"title":"Description de Plasmodium petersi n. sp. et Plasmodium georgesi n. sp., parasites d’un Cercocebus albigena originaire de République Centrafricaine","authors":"J. Poirriez, D. Baccam, E. Dei‐Cas, T. Brogan, I. Landau","doi":"10.1051/PARASITE/1993685203","DOIUrl":"https://doi.org/10.1051/PARASITE/1993685203","url":null,"abstract":"Dans les frottis sanguins colores au Giemsa d’un singe Cercocebus albigena capture en Republique Centrafricaine, trois especes d’hematozoaires ont ete identifiees : Plasmodium gonderi Rodhain et Van Den Berghe, 1936 (nouvel hote et nouvelle localisation geographique), Plasmodium petersi n. sp. et Plasmodium georgesi n. sp.. P. petersi se caracterise par la presence frequente chez les jeunes trophozoites de deux ou trois vacuoles coiffees d’un gros noyau ovalaire, par la morphologie des schizontes presque murs contenant de 12 a 18 noyaux arrondis, homogenes et assez gros, et possedant un cytoplasme bleu clair formant souvent une aureole tres pâle autour de certains noyaux, par la formation d’un pigment en aiguilles noires et par l’apparition tardive de granulations globulaires tres fines, grisâtres, de meme taille et relativement peu nombreuses, dans une hematie augmentee de volume, a bords nets et de teinte normale. P. georgesi n. sp. se caracterise par l’allongement en arc de cercle du noyau de ses trophozoites, par la grande taille et l’aspect polygonal des noyaux des jeunes schizontes immatures (contenant de 2 a 8 noyaux), par le nombre de noyaux des schizontes presque murs (22 a 26), par l’augmentation de volume importante des hematies parasitees qui prennent souvent une forme polygonale, par l’apparition relativement tardive de nombreuses granulations globulaires de tailles irregulieres, devenant de plus en plus violettes et intenses au fur et a mesure que le parasite murit, et par la formation tardive d’un pigment punctiforme jaunâtre.","PeriodicalId":72205,"journal":{"name":"Annales de parasitologie humaine et comparee","volume":"45 1","pages":"203-210"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/PARASITE/1993685203","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"57955449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Nomenclature of Strongylidae above the family group].","authors":"M C Durette-Desset, A G Chabaud","doi":"10.1051/parasite/1993682111","DOIUrl":"https://doi.org/10.1051/parasite/1993682111","url":null,"abstract":"It is necessary to distinguish the three main evolutionary lines which presently constitute the Trichostrongyloidea. We propose to raise the following taxa of Strongylida as presented in the CIH keys (1974). Ancylostomatina for Diaphanocephaloidea and Ancylostomatoidea. Strongylina for Strongyloidea. Trichostrongylina for Trichostrongyloidea. Metastrongylina for Metastrongyloidea. The Trichostronglyina suborder includes three superfamilies: Trichostrongyloidea, Molineoidea superfam. nov. and Heligmosomoidea superfam. nov.","PeriodicalId":72205,"journal":{"name":"Annales de parasitologie humaine et comparee","volume":"68 2","pages":"111-2"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/parasite/1993682111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19205191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Plasmodium berghei: the use of discontinuous urografin density gradients for the separation of exoerythrocytic malaria parasites.","authors":"G François, I Desombere, M Wéry","doi":"10.1051/parasite/1993685220","DOIUrl":"https://doi.org/10.1051/parasite/1993685220","url":null,"abstract":"<p><p>Urografin was used in the lower cushion of discontinuous density gradient systems, for the separation of human hepatoma cells (Hep G2) infected with exoerythrocytic P. berghei forms from uninfected cells. The hepatoma cells exhibited a rather heterogeneous density distribution, masking the possible density differences between infected and uninfected cells and hindering the efficient separation of both cell types. Purely osmotic damage caused by Urografin on human erythrocytes and hepatoma cells is very limited. On the other hand, the direct toxic effects on P. falciparum blood stages and on P. berghei exoerythrocytic stages are very pronounced. The growth of the former forms is partially inhibited after a pretreatment, but remains acceptable if the contact with Urografin is relatively short. It is almost completely blocked during permanent incubation. The latter forms are killed after 1 hour of contact with Urografin.</p>","PeriodicalId":72205,"journal":{"name":"Annales de parasitologie humaine et comparee","volume":"68 5-6","pages":"220-5"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/parasite/1993685220","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19146366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Deniau, R Durand, C Bories, M Paul, A Astier, P Couvreur, R Houin
{"title":"[In vitro study of leishmanicidal agents with drug carriers].","authors":"M Deniau, R Durand, C Bories, M Paul, A Astier, P Couvreur, R Houin","doi":"10.1051/parasite/199368134","DOIUrl":"https://doi.org/10.1051/parasite/199368134","url":null,"abstract":"<p><p>Antileishmanial chemotherapy is hampered by the location of parasites within lysosomal vacuoles of the macrophages which restricts the bioavailability of many potential antileishmanial compounds. In this study, the effectiveness of pentamidine targeted to the infected cells by a linkage to a colloidal drug carrier, methacrylate polymer nanoparticles was explored. In the same way, polyisoalkylcyanoacrylate nanospheres which have, in vitro, trypanolytic properties were also tested. The study was performed in an in vitro model using Leishmania major amastigote stages within the U 937 human monohistiocytic cell line. The antileishmanial activities of unloaded or pentamidine-loaded nanoparticles were compared to those of the free drugs. The 50% effective concentration of targeted pentamidine was 0.10 microgram/ml, while it was up to 2.7 micrograms/ml with the free drug after a 24-hour incubation time. The pentamidine-bound nanoparticles proved to be 25 times more active than the free drug. Unloaded polyisoalkylcyanoacrylate nanoparticles destroyed intracellular amastigote stages (50% EC = 15 micrograms/ml) but at a level close to the cytotoxic concentration.</p>","PeriodicalId":72205,"journal":{"name":"Annales de parasitologie humaine et comparee","volume":"68 1","pages":"34-7"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/parasite/199368134","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19475101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V Thomaz-Soccol, G Lanotte, J A Rioux, F Pratlong, A Martini-Dumas, E Serres
{"title":"Phylogenetic taxonomy of New World Leishmania.","authors":"V Thomaz-Soccol, G Lanotte, J A Rioux, F Pratlong, A Martini-Dumas, E Serres","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":72205,"journal":{"name":"Annales de parasitologie humaine et comparee","volume":"68 2","pages":"104-6"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18695829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Thomaz-Soccol, G. Lanotte, J. Rioux, Francine Pratlong, A. Martini-Dumas, E. Serres
{"title":"Phylogenetic taxonomy of New World Leishmania.","authors":"V. Thomaz-Soccol, G. Lanotte, J. Rioux, Francine Pratlong, A. Martini-Dumas, E. Serres","doi":"10.1051/parasite/1993682104","DOIUrl":"https://doi.org/10.1051/parasite/1993682104","url":null,"abstract":"","PeriodicalId":72205,"journal":{"name":"Annales de parasitologie humaine et comparee","volume":"216 1","pages":"104-6"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83623413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V Thomaz-Soccol, G Lanotte, J A Rioux, F Pratlong, A Martini-Dumas, E Serres
{"title":"Monophyletic origin of the genus Leishmania Ross, 1903.","authors":"V Thomaz-Soccol, G Lanotte, J A Rioux, F Pratlong, A Martini-Dumas, E Serres","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":72205,"journal":{"name":"Annales de parasitologie humaine et comparee","volume":"68 2","pages":"107-8"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19205189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ultrastructural study of Eimeria gastrosauris a coccidium from the stomach epithelium of Australian Geckoes","authors":"I. Paperna","doi":"10.1051/PARASITE/199368270","DOIUrl":"https://doi.org/10.1051/PARASITE/199368270","url":null,"abstract":"Description de la structure fine des trophozoites, de la formation des merozoites, des micro et macrogametocytes et des jeunes oocystes d'Eimeria gastrosauris. Son developpement tissulaire s'effectue dans l'epithelium de la muqueuse gastrique des Geckos australiens Heteronotia binoei et Oedura monilis. La face interne de l'enveloppe de la vacuole parasitophore presente de tres nombreux replis intravacuolaires. A l'exterieur, des microfibrilles s'accumulent frequemment, formant, dans le cytoplasme de la cellule hote, une couche individualisee autour de la vacuole parasitophore. Microgamontes et macrogamontes presentent de profondes invaginations. Les wall forming bodies de type 1 sont tantot lamelaires, tantot granuleux chez le microgamonte. Ceux de type 2 sont formes de deux couches de densite differente","PeriodicalId":72205,"journal":{"name":"Annales de parasitologie humaine et comparee","volume":"68 1","pages":"70-75"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/PARASITE/199368270","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"57954527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Santos-Gomes, P. Abranches, S. Maraghi, M. Dirie, M. C. Silva-Pereira, D. Valverde, D. Molyneux
{"title":"Laboratory and field studies on Herpetosoma Trypanosomes from Portugal","authors":"G. Santos-Gomes, P. Abranches, S. Maraghi, M. Dirie, M. C. Silva-Pereira, D. Valverde, D. Molyneux","doi":"10.1051/PARASITE/1993684163","DOIUrl":"https://doi.org/10.1051/PARASITE/1993684163","url":null,"abstract":"Several small mammals were trapped in the Arrabida region (Portugal) and checked for the presence of trypanosomes which were found in 33 of the 197 (11.1 %) Mus spretus and in 9 of the 29 (31 %) Crocidura russula observed. To our knowledge, this was the first time that trypanosomes were isolated from these mammals species. In the liver of one dead C. russula was observed different parasite forms. The studies of infectivity to experimental rodents, analyses of the DNA buoyant density and the isoenzymatic profils, show that trypanosomes isolates from M. spretus were identical to Trypanosoma musculi isolates from Mus musculus. However the isolates from C. russula , although related to the isolates from murine rodents, were clearely separated from these and close to Trypanosoma microti . These findings may allow further studies on the detection of their vectors and on the study of trypanosome reproduction.","PeriodicalId":72205,"journal":{"name":"Annales de parasitologie humaine et comparee","volume":"68 1","pages":"163-168"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/PARASITE/1993684163","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"57954944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Ghosh, A. Ghosh, S. Basak, S. Adhya, A. Bhattacharya
{"title":"Improved rearing conditions of sandflies (Phlebotomus argentipes), as required for studies on transmission dynamics of leishmaniasis","authors":"K. Ghosh, A. Ghosh, S. Basak, S. Adhya, A. Bhattacharya","doi":"10.1051/PARASITE/1993685244","DOIUrl":"https://doi.org/10.1051/PARASITE/1993685244","url":null,"abstract":"Rearing conditions of sandflies (Phlebotomus argentipes) has been improved. Bloodfed females were initially maintained at 21-23° C for 5-7 days before allowing them to oviposit at 26-28° C and a proportion of flies were directly allowed to oviposit at 26-28° C just after feeding to see their oviposition survival and subsequently the refeeding rate. It was found that when the flies were maintained following the former method gave better results. It is proposed to keep the bloodfed sandflies at a temperature lower than its suitable feeding temperature for a period longer than its oogenesis period and then to bring back the flies at slightly higher temperature, required for oviposition to get better result in oviposition survival and subsequently multiple feeding activity.","PeriodicalId":72205,"journal":{"name":"Annales de parasitologie humaine et comparee","volume":"68 1","pages":"244-246"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/PARASITE/1993685244","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"57955902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}