Advances in laboratory medicine最新文献_第10页

Sangre capilar, más allá de una historia de dinosaurios y unicornios 毛细血管血，超越了恐龙和独角兽的历史

Advances in laboratory medicine Pub Date : 2022-12-01 DOI: 10.1515/almed-2022-0110

Álvaro González, Julia Maroto-García, Nerea Varo

引用次数: 0

Hematology profile analysis in coronavirus disease 2019 (COVID-19) patients. 2019冠状病毒病(COVID-19)患者血液学特征分析

Advances in laboratory medicine Pub Date : 2022-12-01 DOI: 10.1515/almed-2022-0053

Felisia Setio, Darwati Muhadi, Asvin Nurulita, Mansyur Arif, Irawaty Djaharuddin, Arifin Seweng

{"title":"Hematology profile analysis in coronavirus disease 2019 (COVID-19) patients.","authors":"Felisia Setio, Darwati Muhadi, Asvin Nurulita, Mansyur Arif, Irawaty Djaharuddin, Arifin Seweng","doi":"10.1515/almed-2022-0053","DOIUrl":"https://doi.org/10.1515/almed-2022-0053","url":null,"abstract":"Objectives: Some hematological parameters were reported as markers to assess severity of COVID-19 patients. Comorbidities were risk factors for severe COVID-19. Differences in hematology profile based on severity and comorbidity, and correlation between hematology profile and Ct value were never studied at Makassar, Indonesia. The aim of this study were to know the differences of hematology profile based on severity and comorbidity, and the correlation between hematology profile and Ct value in COVID-19 patients.Methods: This study was retrospective, cross-sectional of confirmed COVID-19 patients who had been hospitalized at Dr. Wahidin Sudirohusodo hospital, Makassar, since June to August 2020. Hematology profile, Ct value, comorbidity, and severity of COVID-19 patients were obtained from Hospital Information System Data.Results: From 217 patients, subjects were 102 (47%) male dan 115 (53%) female, 127 mild-moderate patients (58.5%) and 90 severe patients (41.5%), 143 patients (65%) without comorbidity, 74 patients (35%) with comorbidity. White blood cells (WBC), red cell distribution width (RDW), neutrophil and monocyte count, and neutrophil lymphocyte ratio (NLR) were significantly higher in severe patients than mild-moderate patients (p<0.05), besides RBC, hemoglobin, hematocrit, lymphocyte and thrombocyte count were significantly lower in severe patients than mild-moderate patients (p<0.05). Hematology profile was not different significantly based on comorbidity and was not correlated significantly with Ct value, except eosinophil count (r=0.161; p=0.018).Conclusions: We suggest that hematology profile could predict the severity of COVID-19 patients. Moreover, eosinophil count could be considered to predict the infectivity of patient with COVID-19.","PeriodicalId":72097,"journal":{"name":"Advances in laboratory medicine","volume":"3 4","pages":"383-396"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9718295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of oxidative stress in neurodegenerative diseases and potential antioxidant therapies. 氧化应激在神经退行性疾病中的作用和潜在的抗氧化治疗。

Advances in laboratory medicine Pub Date : 2022-12-01 DOI: 10.1515/almed-2022-0111

Paula Sienes Bailo, Elena Llorente Martín, Pilar Calmarza, Silvia Montolio Breva, Adrián Bravo Gómez, Adela Pozo Giráldez, Joan J Sánchez-Pascuala Callau, Juana M Vaquer Santamaría, Anita Dayaldasani Khialani, Concepción Cerdá Micó, Jordi Camps Andreu, Guillermo Sáez Tormo, Isabel Fort Gallifa

{"title":"The role of oxidative stress in neurodegenerative diseases and potential antioxidant therapies.","authors":"Paula Sienes Bailo, Elena Llorente Martín, Pilar Calmarza, Silvia Montolio Breva, Adrián Bravo Gómez, Adela Pozo Giráldez, Joan J Sánchez-Pascuala Callau, Juana M Vaquer Santamaría, Anita Dayaldasani Khialani, Concepción Cerdá Micó, Jordi Camps Andreu, Guillermo Sáez Tormo, Isabel Fort Gallifa","doi":"10.1515/almed-2022-0111","DOIUrl":"https://doi.org/10.1515/almed-2022-0111","url":null,"abstract":"Objectives: The central nervous system (CNS) is essential for homeostasis and controls the physiological functions of the body. However, the biochemical characteristics of the CNS make it especially vulnerable to oxidative damage (OS). This phenomenon compromises correct CNS functioning, leading to neurodegeneration and neuronal death.Contents: OS plays a crucial role in the physiopathology of neurodegenerative diseases. It is involved in multiple mechanisms of nucleic acid, protein, and lipid oxidation, thereby contributing to progressive brain damage. These mechanisms include mitochondrial dysfunction; excessive production of reactive oxygen and nitrogen species; deficiency of antioxidant defenses; protein oligomerization; cytokine production and inflammatory response; blood-brain barrier abnormalities; and proteasome dysfunction. All these dysfunctions are involved in the pathogenesis of neurodegenerative diseases, including Parkinson's disease, Alzheimer's disease, Huntington's disease, or amyotrophic lateral sclerosis.Summary and outlook: A curative treatment is currently not available. Research is focused on the search for therapies that reduce oxidative damage and delay disease progression. In the recent years, researchers have focused their attention on the effects of antioxidant therapies.","PeriodicalId":72097,"journal":{"name":"Advances in laboratory medicine","volume":"3 4","pages":"342-360"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9718293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Beyond the method change in clinical practice: evaluation of insulin-like growth factor I assay. 超越临床实践中的方法变化:胰岛素样生长因子1测定的评价。

Advances in laboratory medicine Pub Date : 2022-12-01 DOI: 10.1515/almed-2021-0069

Paula Sienes Bailo, Marta Fabre Estremera, José Cuenca Alcocel, María Ángeles César Márquez

{"title":"Beyond the method change in clinical practice: evaluation of insulin-like growth factor I assay.","authors":"Paula Sienes Bailo, Marta Fabre Estremera, José Cuenca Alcocel, María Ángeles César Márquez","doi":"10.1515/almed-2021-0069","DOIUrl":"https://doi.org/10.1515/almed-2021-0069","url":null,"abstract":"Objectives: Insulin-like growth factor I (IGF-I) is the preferred biomarker for diagnosing and monitoring growth-related disorders but its serum quantification presents several difficulties since different IGF-I assays still leads to different IGF-I concentrations, especially when results are either above or below the normal range.Methods: We conducted a prospective study between November and December 2020 at a tertiary University Hospital with 212 serum samples to determine the analytical performance of the IGF-I assay on the Cobas e411 (Roche Diagnostics) and compare it with that of the Immulite 2000XPi (Siemens).Results: In this work, we report for the first time the existence of discrepancies between IGF-I levels measured by Immulite 2000XPi and Cobas e411. Deming regression model provided a slope of 1.570 (95% CI: 1.395-1.745) and an intercept of -58.591 (95% CI: -89.151 to -28.030), with R2=0.967 and average bias of +53.061 with overestimation of IGF-I. It was found that Cobas e411 provides abnormally high IGF-I concentrations, but further studies are required to elucidate the cause of the discrepancies.Conclusions: Our data can alert clinicians and laboratory professionals of this situation and avoid misinterpretation of increased IGF-I levels as a therapeutic failure rather than as a problem associated with this method change.","PeriodicalId":72097,"journal":{"name":"Advances in laboratory medicine","volume":"3 4","pages":"397-406"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9718298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Síndrome de Klinefelter con deleción del brazo largo del cromosoma X 克兰费尔特综合征伴X染色体长臂缺失

Advances in laboratory medicine Pub Date : 2022-12-01 DOI: 10.1515/almed-2022-0076

Vanesa Escribano Hernández, M. de Benito, Raffaelle Carraro Casieri

引用次数: 0

Importancia del cateterismo de senos petrosos inferiores en el diagnóstico de síndrome de Cushing, a propósito de un caso 下岩石窦导管在库欣综合征诊断中的重要性，关于一个病例

Advances in laboratory medicine Pub Date : 2022-11-25 DOI: 10.1515/almed-2022-0081

Clara Jiménez García, Paula Sirera Sirera, M. E. Torregrosa Quesada, Victoria González Bueno, Rocío Alfayate Guerra

{"title":"Importancia del cateterismo de senos petrosos inferiores en el diagnóstico de síndrome de Cushing, a propósito de un caso","authors":"Clara Jiménez García, Paula Sirera Sirera, M. E. Torregrosa Quesada, Victoria González Bueno, Rocío Alfayate Guerra","doi":"10.1515/almed-2022-0081","DOIUrl":"https://doi.org/10.1515/almed-2022-0081","url":null,"abstract":"Resumen Objetivos El diagnóstico del síndrome de Cushing (SC) sigue siendo un reto ya que sus síntomas y signos clínicos se superponen a los de otras enfermedades. Caso clínico Varón remitido a Endocrinología por sospecha de SC. Para el estudio del SC se realizaron pruebas de cribado que confirman el hipercortisolimo y pruebas de diagnóstico que confirman un SC-adrenocorticotropina (ACTH) dependiente. Para discriminar entre origen hipofisario y ectópico se realizó el test de estimulación con corticoliberina (CRH). Ante la falta de respuesta en este test y la resonancia magnética nuclear (RMN) negativa se realizó el CSPI que sugería origen hipofisario por lo que se realizó cirugía transesfenoidal. El resultado anatomopatológico confirmó el origen corticotropo del tumor. Conclusiones La investigación etiológica del SC y su diagnóstico diferencial constituye un proceso complejo utilizando diversas pruebas bioquímicas y de imagen. Es importante establecer la secuencia del estudio bioquímico realizando pruebas de cribado, confirmación y localización para establecer origen adrenal, hipofisario o ectópico. A pesar del buen rendimiento diagnóstico, este no es definitivo, especialmente en los casos ACTH dependiente. En nuestro caso, el resultado no concluyente en el test de CRH, requirió un procedimiento invasivo (CSPI) que resultó eficiente en el diagnóstico.","PeriodicalId":72097,"journal":{"name":"Advances in laboratory medicine","volume":"28 1","pages":"411 - 414"},"PeriodicalIF":0.0,"publicationDate":"2022-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83468189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Más allá del cambio metodológico en la práctica clínica: Evaluación de las pruebas de factor de crecimiento insulínico tipo 1 超越临床实践的方法学改变:胰岛素生长因子1型试验的评估

Advances in laboratory medicine Pub Date : 2022-10-17 DOI: 10.1515/almed-2022-0092

P. Sienes Bailo, Marta Fabre Estremera, José Cuenca Alcocel, María Ángeles César Márquez

{"title":"Más allá del cambio metodológico en la práctica clínica: Evaluación de las pruebas de factor de crecimiento insulínico tipo 1","authors":"P. Sienes Bailo, Marta Fabre Estremera, José Cuenca Alcocel, María Ángeles César Márquez","doi":"10.1515/almed-2022-0092","DOIUrl":"https://doi.org/10.1515/almed-2022-0092","url":null,"abstract":"Resumen Objetivos El factor de crecimiento insulínico tipo 1 (IGF-I) es el biomarcador más ampliamente utilizado para el diagnóstico y seguimiento de los trastornos relacionados con el crecimiento, aunque su cuantificación en suero implica una serie de dificultades. De este modo, se han observado discrepancias en las concentraciones de IGF-I según el tipo de ensayo empleado, especialmente en el contexto de concentraciones superiores o inferiores al rango de normalidad. Métodos Entre noviembre y diciembre de 2020, se realizó un estudio prospectivo en un hospital universitario de tercer nivel, en el que se analizaron 212 muestras séricas para determinar la calidad analítica de la prueba de IGF-I cuando se realiza en el analizador Cobas e411 (Roche Diagnostics) y compararla con la de Immulite 2000XPi (Siemens). Resultados El presente es el primer estudio en aportar evidencia sobre la existencia de discrepancias en las concentraciones de IGF-I, según sean medidas con Immulite 2000XPi o Cobas e411. En el análisis de regresión de Deming se obtuvo una pendiente de 1,570 (95% CI: 1,395–1,745), una ordenada en el origen de −58,591 (IC 95%: −89,151 to −28,030), con un R2=0,967 y un sesgo medio de +53,061, con una sobreestimación de los niveles de IGF-I. Observamos que con Cobas e411 se obtienen concentraciones de IGF-I anormalmente elevadas, aunque son necesarios más estudios para dilucidar la causa de dichas discrepancias. Conclusiones Los resultados de este estudio pueden ser de utilidad para alertar a los facultativos, así como a los profesionales de laboratorio de dicha circunstancia, con el fin de evitar la interpretación errónea de niveles aumentados de IGF-I como un fracaso terapéutico en lugar de como un problema asociado a este cambio de método analítico.","PeriodicalId":72097,"journal":{"name":"Advances in laboratory medicine","volume":"38 1","pages":"402 - 406"},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80570732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efectos de la pandemia COVID-19 en la actividad asistencial de los laboratorios clínicos españoles, evolución 2019–2021 2019冠状病毒病大流行对西班牙临床实验室护理活动的影响，演变2019 - 2021

Advances in laboratory medicine Pub Date : 2022-10-13 DOI: 10.1515/almed-2022-0044

Ana Belén Lasierra Monclús, Álvaro González, Francisco A. Bernabéu Andreu, Imma Caballé Martín, Antonio Buño Soto, M. Ibarz, C. González Rodríguez, José Puzo Foncillas

{"title":"Efectos de la pandemia COVID-19 en la actividad asistencial de los laboratorios clínicos españoles, evolución 2019–2021","authors":"Ana Belén Lasierra Monclús, Álvaro González, Francisco A. Bernabéu Andreu, Imma Caballé Martín, Antonio Buño Soto, M. Ibarz, C. González Rodríguez, José Puzo Foncillas","doi":"10.1515/almed-2022-0044","DOIUrl":"https://doi.org/10.1515/almed-2022-0044","url":null,"abstract":"Resumen Objetivos Cuantificar el impacto de la pandemia en la actividad asistencial de los laboratorios clínicos españoles. Métodos Estudio descriptivo, observacional, retrospectivo y multicéntrico. Resultados De marzo a diciembre de 2020 hubo un descenso estadísticamente significativo en el número de solicitudes (−17.7%, p=<0,001) y análisis totales (−18,3%, p<0,001) respecto al mismo periodo de 2019. Se redujo el número de solicitudes de Atención Primaria en un 37,4% (p<0,001) y el número de mediciones de sangre oculta en heces (−45,8%), análisis cualitativo de orina (−30,1%), antígeno prostático específico (PSA) (−28,5%), tirotropina (TSH) (−27,8%), colesterol total (−27,2%) y hemoglobina glicosilada (HbA1c) (−24,7%), p<0,001. Se observó un aumento significativo del número de solicitudes de UCI (76,6%, p<0,001) y del número de mediciones de IL-6 (+22,350,9), dímero-D (+617,2%), troponina (+46,8%) y gasometría arterial (+35,9%), p<0,001. Durante los seis primeros meses de 2021, existieron diferencias significativas para análisis cualitativo de orina (−8,7%, p<0,001), PSA (−6,3%, p=0,009), IL-6 (+66.269,2, p<0,001), dímero-D (+603,6%, p<0,001), troponina (+28,7%, p<0,001), gasometría arterial (+26,2%, p=0,014) y ferritina (+16,0%, p=0,002). Conclusiones Los laboratorios clínicos españoles han sufrido un cambio en el origen de sus solicitudes y en la demanda de pruebas. Se han incrementado aquellas utilizadas en la evaluación y seguimiento de los pacientes COVID-19, y han disminuido las dirigidas al control de los pacientes no-COVID y a cribados poblacionales. El análisis a más largo plazo refleja una recuperación en las pruebas dirigidas al control de las enfermedades crónicas y se mantiene el aumento del número de mediciones de los biomarcadores utilizados en el manejo de los pacientes COVID-19.","PeriodicalId":72097,"journal":{"name":"Advances in laboratory medicine","volume":"243 1","pages":"371 - 382"},"PeriodicalIF":0.0,"publicationDate":"2022-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83642581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustained hyperkalemia in an asymptomatic primary care patient. When to suspect familial pseudohyperkalemia. 无症状初级保健患者的持续高钾血症。何时怀疑家族性假性高钾血症。

Advances in laboratory medicine Pub Date : 2022-10-01 DOI: 10.1515/almed-2022-0057

Carlos Castillo Pérez, Laura Rodríguez Alonso, Adrián Prados Boluda, Marta Cebrián Ballesteros, Blanca Torrubia Dodero

{"title":"Sustained hyperkalemia in an asymptomatic primary care patient. When to suspect familial pseudohyperkalemia.","authors":"Carlos Castillo Pérez, Laura Rodríguez Alonso, Adrián Prados Boluda, Marta Cebrián Ballesteros, Blanca Torrubia Dodero","doi":"10.1515/almed-2022-0057","DOIUrl":"https://doi.org/10.1515/almed-2022-0057","url":null,"abstract":"Objectives: Study and management of a case with elevated potassium levels without apparent clinical causes in successive follow-up visits.Case presentation: We present the case of a primary care female patient who persistently exhibited elevated levels of potassium (5.3-5.9 mmol/L) in successive control laboratory tests, without an apparent clinical cause. The patient was ultimately referred to the Unit of Nephrology, where a potassium-low diet was indicated. Diet did not have any effect on potassium levels. After a thorough study, the cause of hyperkalemia could not be determined.Conclusions: The inconsistency between elevated potassium levels and the reason of consultation, and exclusion of other pre-analytical or pathological causes raised suspicion of familial pseudohyperkalemia. The sample was incubated at different times and temperatures to demonstrate their influence on levels of potassium in blood. Familial pseudohyperkalemia was established as the most probable diagnosis. Finally, the patient was discharged from the Unit of Nephrology and instructed to follow a normal diet.","PeriodicalId":72097,"journal":{"name":"Advances in laboratory medicine","volume":"3 3","pages":"303-312"},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9714572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blue-green neutrophilic inclusion bodies with concurrent liver failure as a predictor of imminent death. 伴有肝功能衰竭的蓝绿中性粒细胞包涵体作为即将死亡的预测因子。

Advances in laboratory medicine Pub Date : 2022-10-01 DOI: 10.1515/almed-2022-0060

Antonio Sierra Rivera, María García Valdelvira, María A Elia Martínez, Ángeles Férez Martí, Goitzane Marcaida Benito

{"title":"Blue-green neutrophilic inclusion bodies with concurrent liver failure as a predictor of imminent death.","authors":"Antonio Sierra Rivera, María García Valdelvira, María A Elia Martínez, Ángeles Férez Martí, Goitzane Marcaida Benito","doi":"10.1515/almed-2022-0060","DOIUrl":"https://doi.org/10.1515/almed-2022-0060","url":null,"abstract":"Objectives: Blue-green, birefractory, poorly-defined, cytoplasmic inclusions in some types of leukocytes are an underdiagnosed finding, which composition and clinical significance is not well understood. Inclusions are only found on peripheral blood smear (PBS).Case presentation: We report the case of a male with a history of chronic disease (hypertension, obesity, dyslipidemia, and kidney failure) admitted to the emergency room (ER) with shortness of breath, who was later transferred to the intensive care unit (ICU). The patient had a torpid disease course and ultimately died of multiorgan failure, including severe liver failure. When his status exacerbated, these inclusions were observed on PBS in conjunction with liver enzyme abnormalities.Conclusions: This case confirms that blue-green inclusions on PBS in cases of acute severe liver failure with concurrent lactic acidosis should be immediately reported to the medical team, since it is suggestive of critical status and poor prognosis.","PeriodicalId":72097,"journal":{"name":"Advances in laboratory medicine","volume":"3 3","pages":"295-302"},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9719870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1