发布求助
文献互助 智能选刊 最新文献

分子植物育种最新文献

筛选
英文 中文
甘薯IbSPF1基因植物表达载体的构建 甘薯IbSPF1基因植物表达载体的构建
分子植物育种 Pub Date : 2011-01-01 DOI: 10.5376/mpb.cn.2011.09.0082
后猛
{"title":"甘薯IbSPF1基因植物表达载体的构建","authors":"后猛","doi":"10.5376/mpb.cn.2011.09.0082","DOIUrl":"https://doi.org/10.5376/mpb.cn.2011.09.0082","url":null,"abstract":"为了获得具有多种抗逆特性的转基因甘薯材料，本文构建了由甘薯过氧化物胁迫诱导型启动子SWPA2和植物组成型启动子CaMV 35S分别控制、且含有甘薯SP8的结合蛋白基因( IbSPF1 )的过表达植物载体。IbSPF1是植物WRKY转录因子家族的成员之一。由于其参与植物多种生物和非生物胁迫的反应以及许多生理过程，WRKY类家族越来越成为人们研究的热点。本研究利用RT-PCR方法从甘薯茎叶组织中扩增出编码 IbSPF1 的完整开放阅读框，与已发表的 IbSPF1 相比，从翻译起始点开始第1 009位点的胸腺嘧啶转变为胞嘧啶，从而使翻译产物由色氨酸转变为脯氨酸。本研究进一步构建了由不同启动子控制、携带 IbSPF1 基因的超表达植物载体pCAMBIA2300，并转入到根癌农杆菌菌株EHA105中，为未来甘薯抗逆转基因打下基础。","PeriodicalId":71881,"journal":{"name":"分子植物育种","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147901435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
牡丹花芽休眠解除过程中实时定量PCR内参基因的选择 牡丹花芽休眠解除过程中实时定量PCR内参基因的选择
分子植物育种 Pub Date : 2011-01-01 DOI: 10.5376/mpb.cn.2011.09.0007
张玉喜
{"title":"牡丹花芽休眠解除过程中实时定量PCR内参基因的选择","authors":"张玉喜","doi":"10.5376/mpb.cn.2011.09.0007","DOIUrl":"https://doi.org/10.5376/mpb.cn.2011.09.0007","url":null,"abstract":"","PeriodicalId":71881,"journal":{"name":"分子植物育种","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147899215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
基于SRAP技术对浙南柚类地方资源遗传多样性分析和鉴定 基于SRAP技术对浙南柚类地方资源遗传多样性分析和鉴定
分子植物育种 Pub Date : 2011-01-01 DOI: 10.5376/mpb.cn.2011.09.0044
陈巍
{"title":"基于SRAP技术对浙南柚类地方资源遗传多样性分析和鉴定","authors":"陈巍","doi":"10.5376/mpb.cn.2011.09.0044","DOIUrl":"https://doi.org/10.5376/mpb.cn.2011.09.0044","url":null,"abstract":"","PeriodicalId":71881,"journal":{"name":"分子植物育种","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147903729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
雌雄异花甜瓜经AgNO3诱导后的雄蕊发育和差异基因表达 雌雄异花甜瓜经AgNO3诱导后的雄蕊发育和差异基因表达
分子植物育种 Pub Date : 2011-01-01 DOI: 10.5376/mpb.cn.2011.09.0010
李晓明
{"title":"雌雄异花甜瓜经AgNO3诱导后的雄蕊发育和差异基因表达","authors":"李晓明","doi":"10.5376/mpb.cn.2011.09.0010","DOIUrl":"https://doi.org/10.5376/mpb.cn.2011.09.0010","url":null,"abstract":"本研究利用cDNA-AFLP技术对AgNO 3 诱导后的甜瓜雌雄异花系'RH107'mRNA进行指纹图谱分析，320对引物组合共分离到差异表达的TDF (transcription derived fragment, 转录衍生片段) 131个。对其中的28个TDF进行了克隆、测序和序列分析。研究结果表明，在获得的16个有效序列中，6个与GenBank中功能已知基因同源性在72%和100%之间，其功能涉及信号传导、转录调控和能量代谢等；5个与功能未知蛋白的核苷酸序列或氨基酸序列有同源性；2个与已知表达序列标签(EST)有同源性，但功能未知；3个未找到同源序列。由此推断，Ag + 诱导表达差异片段可能分别参与了信号转导、基因表达调控和能量代谢等过程，从而参与调节花器官性别分化。","PeriodicalId":71881,"journal":{"name":"分子植物育种","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147913902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
甘薯淀粉合成关键酶及其基因结构与功能的研究进展 甘薯淀粉合成关键酶及其基因结构与功能的研究进展
分子植物育种 Pub Date : 2011-01-01 DOI: 10.5376/mpb.cn.2011.09.0065
唐维
{"title":"甘薯淀粉合成关键酶及其基因结构与功能的研究进展","authors":"唐维","doi":"10.5376/mpb.cn.2011.09.0065","DOIUrl":"https://doi.org/10.5376/mpb.cn.2011.09.0065","url":null,"abstract":"","PeriodicalId":71881,"journal":{"name":"分子植物育种","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
瓜叶菊F3H同源基因表达与花青素苷合成的相关性分析 瓜叶菊F3H同源基因表达与花青素苷合成的相关性分析
分子植物育种 Pub Date : 2011-01-01 DOI: 10.5376/mpb.cn.2011.09.0068
孙翊
{"title":"瓜叶菊F3H同源基因表达与花青素苷合成的相关性分析","authors":"孙翊","doi":"10.5376/mpb.cn.2011.09.0068","DOIUrl":"https://doi.org/10.5376/mpb.cn.2011.09.0068","url":null,"abstract":"为了探讨瓜叶菊黄烷酮3-羟基化酶(flavanone 3-hydroxylase, F3H)在类黄酮生物合成途径中的作用，本文作者以瓜叶菊( Pericallis cruenta L′Herit; Senecio cruentus Masson ex L′Herit)不同色系品种作为实验材料，分析基因表达与色素成分之间的关系。类黄酮成分的紫外-可见吸收光谱分析表明，红色系、紫色系、蓝色系舌状花中既含有花青素又含有花黄素，而白色系舌状花中仅含有花黄素。Southern blot检测发现， F3H 同源基因在不含有花青素苷的白色和含有花青素苷的蓝色瓜叶菊基因组上均至少分布有2个拷贝。Northern blot分析结果显示，两种颜色的瓜叶菊舌状花中均具有 F3H 同源基因的转录本，且表达水平均相同，与是否含有花青素苷并不直接相关。上述研究结果表明：瓜叶菊中 F3H 同源基因可能不仅参与了花青素苷的合成，还参与了花黄素的合成。根据这一研究结果还可以推测出，白色系与其他色系瓜叶菊之间的花色差异是在 F3H 基因之后的合成步骤中产生的，因此瓜叶菊白色系品种可以作为研究花青素苷合成途径中 F3H 基因下游其他基因功能的良好受体材料。","PeriodicalId":71881,"journal":{"name":"分子植物育种","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147898560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
落叶松总DNA的提取及ISSR-PCR引物筛选 落叶松总DNA的提取及ISSR-PCR引物筛选
分子植物育种 Pub Date : 2011-01-01 DOI: 10.5376/mpb.cn.2011.09.0077
李红艳
{"title":"落叶松总DNA的提取及ISSR-PCR引物筛选","authors":"李红艳","doi":"10.5376/mpb.cn.2011.09.0077","DOIUrl":"https://doi.org/10.5376/mpb.cn.2011.09.0077","url":null,"abstract":"","PeriodicalId":71881,"journal":{"name":"分子植物育种","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147886993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
茶树儿茶素酯化分子机理与研究途径 茶树儿茶素酯化分子机理与研究途径
分子植物育种 Pub Date : 2011-01-01 DOI: 10.5376/mpb.cn.2011.09.0071
张广辉
{"title":"茶树儿茶素酯化分子机理与研究途径","authors":"张广辉","doi":"10.5376/mpb.cn.2011.09.0071","DOIUrl":"https://doi.org/10.5376/mpb.cn.2011.09.0071","url":null,"abstract":"","PeriodicalId":71881,"journal":{"name":"分子植物育种","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147911170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
水稻叶片与光合效率的相关特性研究分析 水稻叶片与光合效率的相关特性研究分析
分子植物育种 Pub Date : 2011-01-01 DOI: 10.5376/mpb.cn.2011.09.0054
来凯凯
{"title":"水稻叶片与光合效率的相关特性研究分析","authors":"来凯凯","doi":"10.5376/mpb.cn.2011.09.0054","DOIUrl":"https://doi.org/10.5376/mpb.cn.2011.09.0054","url":null,"abstract":"","PeriodicalId":71881,"journal":{"name":"分子植物育种","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147885850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
内含子调控转基因植物外源基因的表达研究 内含子调控转基因植物外源基因的表达研究
分子植物育种 Pub Date : 2011-01-01 DOI: 10.5376/mpb.cn.2011.09.0012
谢树章
{"title":"内含子调控转基因植物外源基因的表达研究","authors":"谢树章","doi":"10.5376/mpb.cn.2011.09.0012","DOIUrl":"https://doi.org/10.5376/mpb.cn.2011.09.0012","url":null,"abstract":"","PeriodicalId":71881,"journal":{"name":"分子植物育种","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147881796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信
小红书