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高羊茅RNA提取方法的比较研究 高羊茅RNA提取方法的比较研究
分子植物育种 Pub Date : 2010-01-01 DOI: 10.5376/mpb.cn.2010.08.0007
王康
{"title":"高羊茅RNA提取方法的比较研究","authors":"王康","doi":"10.5376/mpb.cn.2010.08.0007","DOIUrl":"https://doi.org/10.5376/mpb.cn.2010.08.0007","url":null,"abstract":"","PeriodicalId":71881,"journal":{"name":"分子植物育种","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147905762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
EAR型转录抑制子和嵌合抑制子沉默技术研究进展及应用 EAR型转录抑制子和嵌合抑制子沉默技术研究进展及应用
分子植物育种 Pub Date : 2010-01-01 DOI: 10.5376/mpb.cn.2010.08.0012
刘坤
{"title":"EAR型转录抑制子和嵌合抑制子沉默技术研究进展及应用","authors":"刘坤","doi":"10.5376/mpb.cn.2010.08.0012","DOIUrl":"https://doi.org/10.5376/mpb.cn.2010.08.0012","url":null,"abstract":"","PeriodicalId":71881,"journal":{"name":"分子植物育种","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147882287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Shotgun 蛋白质组学技术在致病性烟草青枯菌分泌蛋白研究中的应用 Shotgun 蛋白质组学技术在致病性烟草青枯菌分泌蛋白研究中的应用
分子植物育种 Pub Date : 2010-01-01 DOI: 10.5376/mpb.cn.2010.08.0005
宋浩
{"title":"Shotgun 蛋白质组学技术在致病性烟草青枯菌分泌蛋白研究中的应用","authors":"宋浩","doi":"10.5376/mpb.cn.2010.08.0005","DOIUrl":"https://doi.org/10.5376/mpb.cn.2010.08.0005","url":null,"abstract":"","PeriodicalId":71881,"journal":{"name":"分子植物育种","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147888076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
三种抗生素对落叶松胚性细胞系生长的影响 三种抗生素对落叶松胚性细胞系生长的影响
分子植物育种 Pub Date : 2010-01-01 DOI: 10.5376/mpb.cn.2010.08.0015
朱彩虹
{"title":"三种抗生素对落叶松胚性细胞系生长的影响","authors":"朱彩虹","doi":"10.5376/mpb.cn.2010.08.0015","DOIUrl":"https://doi.org/10.5376/mpb.cn.2010.08.0015","url":null,"abstract":"","PeriodicalId":71881,"journal":{"name":"分子植物育种","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147884874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
叶酸生物合成途径中调控基因folB表达载体的构建及在大豆中的转化 叶酸生物合成途径中调控基因folB表达载体的构建及在大豆中的转化
分子植物育种 Pub Date : 2006-01-01 DOI: 10.5376/mpb.cn.2015.13.0010
张原宇
{"title":"叶酸生物合成途径中调控基因<i>folB</i>表达载体的构建及在大豆中的转化","authors":"张原宇","doi":"10.5376/mpb.cn.2015.13.0010","DOIUrl":"https://doi.org/10.5376/mpb.cn.2015.13.0010","url":null,"abstract":"叶酸是一种水溶性B族维生素，是维持人体正常活动所必需的营养元素之一，也是植物体内参与一碳单元反应的重要辅酶。 folB 基因是叶酸调控基因，来源于拟南芥，调控叶酸合成途径中的关键酶DHNA的生成。本研究将人工合成的 folB 基因转入大豆受体材料Williams82中。共侵染200个大豆外植体，得到26株转化苗。通过对转化苗的草丁膦(浓度150 mg/L)涂抹鉴定，获得16株除草剂抗性转化苗。通过PCR检测，得到12株PCR阳性转化苗。经过 bar 试纸条检测，其中10株为阳性。Southern杂交结果表明，9株转化苗为Southern杂交阳性。研究结果表明：外源功能基因 folB 和筛选标记基因 bar 已经整合到大豆基因组中。相应的转基因后代表现出除草剂抗性和 folB 基因Southern杂交阳性。本研究通过将 folB 基因转入大豆，获得了转基因后代，以此为基础可进一步选育高叶酸含量的转基因大豆品种。","PeriodicalId":71881,"journal":{"name":"分子植物育种","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147906934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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