{"title":"Ante-mortem diagnosis of unilateral pulmonary vein stenosis in a cat: a case report.","authors":"Takuma Aoki, Takashi Miyamoto, Kota Kizaki, Asuka Ueshima, Kentaro Iwasaki, Takuya Kusaka, Haruko Terui","doi":"10.1186/s13028-025-00803-y","DOIUrl":"https://doi.org/10.1186/s13028-025-00803-y","url":null,"abstract":"<p><strong>Background: </strong>Pulmonary hypertension (PH) detection in cats may be challenging. Pulmonary venous stenosis (PVS) is rare in cats and can lead to PH. The only reported PVS case received a post-mortem diagnosis. Imaging during the cat's lifetime established the diagnosis in this case.</p><p><strong>Case presentation: </strong>A 2 year-old Norwegian Forest cat was diagnosed with pulmonary oedema and PH secondary to cor triatriatum sinister (CTS) and showed improved breathing following two subcutaneous furosemide treatments, 1 and 2 mg/kg, during an overnight stay at the referral veterinary hospital. Sildenafil alone (0.69 mg/kg, PO, BID) was prescribed post-discharge to address PH without diuretics. Post-discharge from the referral veterinary hospital, collapse and pre-syncope were suspected to be due to PH. Consequently, sildenafil was titrated weekly, starting at 1.09 mg/kg BID and increasing to 1.63 mg/kg BID. Pre-syncope and collapse resolved, and pulmonary opacities reduced considerably, although concerns remained that increased pulmonary blood flow to suspected CTS from sildenafil might worsen cardiogenic pulmonary oedema. The patient was also treated with rivaroxaban (2.5 mg/head, SID), considering the increased risk of thrombus formation due to blood flow stasis and endothelial damage. Thirty-eight days later, the cat presented for the first time to our hosipital (Azabu University Veterinary Teaching Hospital) for examination. On echocardiography, a continuous mosaic blood flow (maximum and minimum velocity, 3.14 m/s; estimated pressure gradient, 39.4 mmHg) was observed in two enlarged pulmonary veins. Pulmonary artery enlargement (main pulmonary artery to thoracic aorta ratio: 1.90), pulmonary vein stenosis (PVS), and diffuse bilateral ground-glass lung opacities were observed using computed tomography. PH with unilateral PVS involving two out of the three right pulmonary veins, specifically the right cranial and right middle pulmonary veins, along with pulmonary parenchymal disease, was diagnosed. The cat was further treated with furosemide (1 mg/kg, BID, PO) with no clinical signs but succumbed to acute dyspnoea 51 days after the first visit.</p><p><strong>Conclusions: </strong>Unilateral PVS should be considered in young cats with a localised alveolar pattern and no left atrial enlargement, because the prognosis may be poor. Severe PH with PVS may coexist with lung disease. If sildenafil is used, it should be started at a low dose and monitored closely.</p>","PeriodicalId":7181,"journal":{"name":"Acta Veterinaria Scandinavica","volume":"67 1","pages":"21"},"PeriodicalIF":1.9,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eva Axnér, Josefine Jakobsson, Tilde Vermelin, Ulrika Hermansson
{"title":"A retrospective study on dystocia in the cat, evaluation of 111 cases.","authors":"Eva Axnér, Josefine Jakobsson, Tilde Vermelin, Ulrika Hermansson","doi":"10.1186/s13028-025-00805-w","DOIUrl":"https://doi.org/10.1186/s13028-025-00805-w","url":null,"abstract":"<p><p>Dystocia affects on average 3-8% of all pregnancies in purebred cats. Nonpedigree cats are also affected, but the incidence is unknown. The causes of dystocia and the optimal treatment are largely unexplored in cats. The aims of the present retrospective study were to describe feline dystocia cases and to evaluate kitten mortality in relation to factors associated with dystocia in cats. Medical records of 111 cases (107 queens) treated for dystocia from 2017 to 2024 were retrieved from client files at the University Animal Hospital in Uppsala, Sweden. At the initiation of treatment, 276 kittens remained in utero or in the birth canal. The total kitten mortality rate, including that of kittens born before treatment but excluding four kittens that were euthanized at the owner's request, was 40.9%. The mortality of kittens born after treatment was 44.1%, excluding four kittens euthanized at the owner's request. Two queens died, one of which was euthanized at the owner's request. Among all the cases, 91 (82.0%) were surgically treated, with caesarean section, or en bloc resection in two patients. Ovariohysterectomy was performed in 47.2% of the queens that were surgically treated. Medical treatment was initiated in 30 patients, and was successful in 11 of them, and 19 were further surgically treated after only partial or no success. The success rate of medical treatment was thus 36.7%. Eight queens were hypocalcaemic. Maternal hyperglycaemia was present in 65.5% of the cases and significantly increased the risk of kitten mortality. The estimated duration of second-stage labour before admission did not affect kitten mortality. Disturbed labour (total or partial uterine inertia) was the most common cause of dystocia. Feline dystocia was associated with high kitten mortality but low mortality in queens. Most queens with dystocia were treated surgically, but medical treatment with calcium and/or oxytocin was efficient in cases with non-obstructive dystocia, and ≤ 3 foetuses remaining. Hypocalcaemia may contribute to dystocia in a minority of cases. Maternal hyperglycaemia increased the risk of mortality before discharge. Diagnosing dystocia may be challenging in cats, as there is no clear association between the length of the parturition process and mortality.</p>","PeriodicalId":7181,"journal":{"name":"Acta Veterinaria Scandinavica","volume":"67 1","pages":"20"},"PeriodicalIF":1.9,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11993979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Josefine Jerlström, Ann-Kristina Lind, Cecilia Lindahl, Charlotte Berg, Anna Wallenbeck
{"title":"Traumatic injuries detected at slaughter in cattle: impact of production system and season on animal welfare and meat condemnation in Sweden.","authors":"Josefine Jerlström, Ann-Kristina Lind, Cecilia Lindahl, Charlotte Berg, Anna Wallenbeck","doi":"10.1186/s13028-025-00804-x","DOIUrl":"https://doi.org/10.1186/s13028-025-00804-x","url":null,"abstract":"<p><p>The purpose of ante- and post-mortem inspections at slaughterhouses is to ensure that meat and other relevant food products of animal origin are safe for human consumption. However, these inspections can also be useful for detecting animal health and welfare issues. In cattle, traumatic injuries from on-farm incidents, transport or handling at the slaughterhouse are indications of both reduced animal welfare and increased risk of food waste, ultimately resulting in economic losses for both farmers and slaughterhouses. This observational study aimed to investigate the prevalence and seasonal variation of traumatic injuries in cows and heifers reared on organic and conventional farms in Sweden. The study includes slaughter remarks and condemnations from meat inspection data from 336,071 animals slaughtered between 2020 and 2022. Two types of injuries were analysed: \"chronic traumatic injuries\" (CTI) sustained on-farm and \"acute traumatic injuries\" (ATI) sustained during transport or at the slaughterhouse. Logistic regression models were developed to assess the influence of production system and season. Results show a higher prevalence of CTI in animals from conventional farms (9.8%) compared to organic farms (6.9%; P < 0.001), which may indicate that animals from organic farms are managed and handled in a way that makes them better prepared for challenges that they are later exposed to on-farm prior to slaughter. ATI were more frequent in animals from organic farms during the grazing period (interaction between production system and season: P = 0.002), which may indicate that animals from organic farms find the transition to the slaughterhouse environment more abrupt and stressful during the grazing period. Condemnations due to injuries were significantly higher for animals with CTI or ATI compared to animals without these specific remarks. These findings highlight the importance of pre-slaughter management, both on-farm and at the slaughterhouse, and slaughterhouse design in improving animal welfare and reducing food as well as economic losses associated with carcase condemnations.</p>","PeriodicalId":7181,"journal":{"name":"Acta Veterinaria Scandinavica","volume":"67 1","pages":"18"},"PeriodicalIF":1.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11987234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Deborah Johanna Eikelberg, Melanie Stoff, Martin Peters, Marko Legler, Ingo Gerhauser, Peter Wohlsein
{"title":"Comparative immunohistochemical characterisation of a teratoma in a domestic duck (Anas platyrhynchos) and a teratocarcinoma in a muscovy duck (Cairina moschata).","authors":"Deborah Johanna Eikelberg, Melanie Stoff, Martin Peters, Marko Legler, Ingo Gerhauser, Peter Wohlsein","doi":"10.1186/s13028-025-00791-z","DOIUrl":"https://doi.org/10.1186/s13028-025-00791-z","url":null,"abstract":"<p><strong>Background: </strong>Neoplasms consisting of two or more germinal layers are classified as teratomas if benign or as teratocarcinomas if malignant. Teratomas are rare tumours occurring in a wide range of species including mammals, birds and reptiles and only a few cases have been reported in ducks with infrequent documentation of immunohistochemical characterisation. Teratocarcinomas occur rarely and, to the authors' knowledge, have not been described or immunohistochemically characterised in ducks, yet. Therefore, the clinical and pathological presentation of a teratoma in a mulard duck and a teratocarcinoma in a muscovy duck are described. In addition to a histologic examination, both tumours were characterised by applying a panel of immunohistochemical markers previously tested on duck tissue.</p><p><strong>Case presentation: </strong>A mulard duck (Anas platyrhynchos x Cairina moschata) showed a mass in the cranial coelomic cavity histologically diagnosed as tridermic teratoma. A caudal coelomic mass in a Muscovy duck (Cairina moschata) was histologically diagnosed as a teratocarcinoma with metastases to the liver, mesentery and intestinal wall. An extensive immunohistochemical examination for a detailed characterisation and comparison with duck control tissue was performed in both cases, highlighting various components of the neoplastic tissues including lymphocytes, nervous and endodermal components.</p><p><strong>Conclusions: </strong>To the authors' knowledge, this is the first report of a teratocarcinoma in a duck with intense immunohistochemical characterisation and comparison with a teratoma in another duck. Immunohistochemistry enables a more profound examination of the histogenetic composition of such neoplasms compared to histology alone. Both neoplasms should be considered as differentials for masses in body cavities of ducks. During these examinations, a large spectrum of antibodies specific for different tissues and cells were tested on duck control tissue which can be of help for immunohistochemical examinations on avian tissues in the future.</p>","PeriodicalId":7181,"journal":{"name":"Acta Veterinaria Scandinavica","volume":"67 1","pages":"19"},"PeriodicalIF":1.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11987307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hanna Nurmi, Laura Hänninen, Sauli Laaksonen, Anna Valros
{"title":"The effect of a non-steroidal anti-inflammatory drug on the locomotor activity of reindeer (Rangifer tarandus tarandus) on their natural pastures after clamp castration-a pilot study.","authors":"Hanna Nurmi, Laura Hänninen, Sauli Laaksonen, Anna Valros","doi":"10.1186/s13028-025-00802-z","DOIUrl":"https://doi.org/10.1186/s13028-025-00802-z","url":null,"abstract":"<p><strong>Background: </strong>During seasonal round-ups, free-grazing reindeer are gathered from natural pastures. Reindeer bulls removed from breeding are clamp castrated, traditionally without analgesia, and then returned to the grazing grounds. The new Finnish Animal Welfare Act requires the use of analgesia in painful procedures. Our earlier studies have shown that a single dose of the non-steroidal anti-inflammatory drug (NSAID) meloxicam may maintain therapeutic plasma concentrations for 2-3 days in reindeer. No studies have been conducted on the effect of meloxicam on the locomotor activity of free-ranging castrated reindeer after castration. We installed GPS collars on 16 male reindeer (at least 5 years old, 130-160 kg), chosen to be castrated as a standard procedure during the round-up held on 5 Oct 2020. Of these, eight were randomly selected to receive approximately 0.5 mg/kg of meloxicam subcutaneously (NSAID group) and eight received no analgesia (TRAD group). The trackers were set to provide location twice per hour with 10 m accuracy. From the GPS data, we calculated the daily distances travelled by the reindeer during the 3 days after castration and analysed the differences between the treatments using a GEE model. Fixed factors were treatment (NSAID or TRAD), days (1-3) and hours, and the interactions between these variables. Our key presumption was that a meloxicam injection can reduce the pain related restless locomotion of newly clamp castrated reindeer.</p><p><strong>Results: </strong>The overall mean ± SE daily distances travelled by NSAID (n = 8) and TRAD (n = 8) reindeer did not differ (6.60 ± 0.67 km vs. 8.60 ± 1.54 km). However, all reindeer (n = 16) moved more on day 1 than day 3. TRAD reindeer travelled farther than NSAID on day 1 (11.67 ± 2.25 km vs. 7.08 ± 0.61 km, P < 0.05), but no differences were observed on days 2 or 3 due to high variation (10.19 ± 3.87 km vs. 6.59 ± 0.85 km and 5.35 ± 0.39 km vs. 6.17 ± 0.70 m, P > 0.1). NSAID movement remained stable between the days (P > 0.1), while TRAD activity declined (P = 0.002), levelling with NSAID by day 3. Daytime distances exceeded nighttime distances on days 2 and 3, with TRAD showing more disrupted daily rhythms.</p><p><strong>Conclusions: </strong>Meloxicam may reduce restlessness in newly castrated reindeer, changing postoperative locomotor activity patterns in a way that suggests pain alleviation during the first 2-3 days following clamp castration. Further studies are needed on the use of analgesia and GPS collars for pain monitoring in freely grazing reindeer.</p>","PeriodicalId":7181,"journal":{"name":"Acta Veterinaria Scandinavica","volume":"67 1","pages":"17"},"PeriodicalIF":1.9,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11983737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Estelle Ågren, Annette Backhans, Maria Lindberg, Marie Sjölund, Björn Bengtsson, Arianna Comin
{"title":"Antimicrobial resistance in Escherichia coli from Swedish piglets with diarrhoea and associations with potential risk factors.","authors":"Estelle Ågren, Annette Backhans, Maria Lindberg, Marie Sjölund, Björn Bengtsson, Arianna Comin","doi":"10.1186/s13028-025-00795-9","DOIUrl":"10.1186/s13028-025-00795-9","url":null,"abstract":"<p><strong>Background: </strong>Antibiotic treatments of diarrhoea in suckling piglets and in pigs after weaning are common worldwide and contribute to antimicrobial resistance (AMR) in Escherichia coli from pigs. In Sweden, during the last decades, resistance to trimethoprim-sulphonamide and ampicillin has increased markedly in E. coli from routine clinical samples from piglets with diarrhoea, hereafter referred to as \"clinical submissions\". This has occurred despite a comparatively low use of antibiotics in Swedish pig production. However, clinical submissions might be biased towards farms with treatment failures and therefore overestimate occurrence of AMR. To explore the representativeness of data from such samples we compared occurrence of AMR in E. coli from clinical submissions and from concurrent samples collected from piglets with diarrhoea by convenience, referred to as \"study samples\". We also investigated associations between farm-related potential risk factors and AMR using farm data collected through a questionnaire. Data were evaluated using univariable and multivariable statistical models, as well as a multivariate model.</p><p><strong>Results: </strong>In all, 158 study samples from 97 herds and questionnaires from 83 herds were analysed. Resistance to streptomycin (37%), trimethoprim-sulphonamide (32%), ampicillin (30%), and tetracycline (18%) were the most frequent traits. Occurrence of AMR in 158 E. coli isolates from study samples was not significantly different from occurrence in 57 isolates from concurrent clinical submissions (P > 0.05). In 70% of herds, more than 10% of the sows were treated with antibiotics in the first week after farrowing, and trimethoprim-sulphonamide was the most common first choice antibiotic. Trimethoprim-sulphonamide resistance was associated with the proportion of sows receiving post-farrowing treatment. Resistance to ampicillin, tetracycline, and streptomycin resistances were indirectly associated with sow treatments, likely via co-resistance to trimethoprim-sulphonamide. There was no significant association between high dose zinc oxide supplementation and AMR (P > 0.05).</p><p><strong>Conclusions: </strong>Clinical submissions do not overestimate occurrence of AMR in E. coli from Swedish piglets with diarrhoea and are therefore relevant for AMR monitoring. Even at low treatment rates, post-farrowing treatment of sows increases the risk for AMR in piglets. This applies especially for trimethoprim-sulphonamide resistance, but also for resistance to other antibiotics, and indicates that antibiotic use must be reduced substantially to achieve a reduction of AMR.</p>","PeriodicalId":7181,"journal":{"name":"Acta Veterinaria Scandinavica","volume":"67 1","pages":"16"},"PeriodicalIF":1.9,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11967044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anne Marie Michelsen, Nina Dam Otten, Mogens Vestergaard, Liza Rosenbaum Nielsen, Bodil Højlund Nielsen, Henrik Laessøe Martin, Nynne Capion, Mette Bisgaard Petersen
{"title":"Evaluation of passive immunity transfer in Danish dairy calves measured by enzyme-linked immunosorbent assay and Brix refractometer.","authors":"Anne Marie Michelsen, Nina Dam Otten, Mogens Vestergaard, Liza Rosenbaum Nielsen, Bodil Højlund Nielsen, Henrik Laessøe Martin, Nynne Capion, Mette Bisgaard Petersen","doi":"10.1186/s13028-025-00801-0","DOIUrl":"10.1186/s13028-025-00801-0","url":null,"abstract":"<p><p>Calves are born agammaglobulinemic and depend on transfer of passive immunity from colostrum. Failure of transfer of passive immunity (FTPI) and adequate transfer of passive immunity (ATPI) are defined as serum immunoglobulin G (IgG) levels < 10 and ≥ 10 mg/mL, respectively. The objective of this study was to evaluate the level of passively transferred immunity in Danish dairy calves measured with Enzyme-linked immunosorbent assay (ELISA) and Brix refractometer. Blood samples from 834 calves (430 bull calves and 404 heifer calves) aged two to nine days were included in the study. Serum IgG concentration was determined by ELISA and percentage Brix (%Brix) with a digital refractometer. Median serum IgG concentration was 17.05 mg/mL and mean %Brix was 8.5%. A total of 592 (71.0%) and 242 samples (29.0%) had a serum IgG concentration ≥ 10 mg/mL and < 10 mg/mL, respectively. For %Brix 541 (64.9%) and 293 (35.1%) was ≥ 8.1 and < 8.1%, respectively. Serum IgG concentrations and %Brix measurements were highly correlated (r = 0.88). The level of passive immunity in Danish dairy calves seems low and does not meet new recommendations. However, applying cut-offs based on radial immunodiffusion to serum IgG concentrations derived from ELISA are not straightforward and determination of new cut-off values for FTPI based on ELISA are recommended.</p>","PeriodicalId":7181,"journal":{"name":"Acta Veterinaria Scandinavica","volume":"67 1","pages":"15"},"PeriodicalIF":1.9,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kehamo Abi, Youwen Yang, Chen Yang, Kegu Ji'e, Falong Yang
{"title":"Analysis for Bopivirus B in goats in the Sichuan province, China using a novel TaqMan real-time polymerase chain reaction assay.","authors":"Kehamo Abi, Youwen Yang, Chen Yang, Kegu Ji'e, Falong Yang","doi":"10.1186/s13028-024-00777-3","DOIUrl":"10.1186/s13028-024-00777-3","url":null,"abstract":"<p><strong>Background: </strong>Bopivirus B is an emerging picornavirus that affects goats in China. This study aimed to establish a TaqMan real-time PCR assay for detecting Bopivirus B and conduct a preliminary survey of infection in six goat farms in Sichuan province, China. Specific primers and a probe targeting the 3D gene of Bopivirus B were designed, and the TaqMan-based real-time PCR assay was successfully established following the optimization of reaction conditions and components. A total of 257 goat fecal samples were collected from six farms in Sichuan and tested using the newly developed method.</p><p><strong>Results: </strong>The assay demonstrated a linear relationship between 2.73 × 10<sup>3</sup> and 2.73 × 10<sup>9</sup> copies/µL, with a high correlation coefficient (R<sup>2</sup> = 0.999) and amplification efficiency of 109%. Additionally, the assay exhibited excellent specificity and reproducibility, with a detection limit of 27.3 copies/µL. The field positive rate of Bopivirus B was 100%, and a higher positive rate was observed in diarrheal fecal samples (33.72%) compared to non-diarrheal fecal samples (12.28%, P < 0.005), suggesting a potential association between Bopivirus B and goat diarrhea, with a widespread prevalence in goats in the Sichuan province. Furthermore, ten complete 3D genes sequences of Bopivirus B were obtained, and phylogenetic analysis showed that all Bopivirus B strains in this study were most closely related to two known Chinese Bopivirus strains based on nucleotide sequences of the 3D gene.</p><p><strong>Conclusions: </strong>This study developed a highly specific, repeatable, and sensitive TaqMan-based real-time PCR assay targeting the 3D gene for Bopivirus B detection, offering a valuable tool for the detection and epidemiological investigation of Bopivirus B. The prevalence of Bopivirus B was widespread in goats in China, with a close association observed between Bopivirus B and goat diarrhea.</p>","PeriodicalId":7181,"journal":{"name":"Acta Veterinaria Scandinavica","volume":"67 1","pages":"13"},"PeriodicalIF":1.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Katrine Vestergaard Kristiansen, Anders Simon Schrøder, Dorothee Bienzle, Tanja Vedel, Jørgen Steen Agerholm, Mette Berendt
{"title":"Lumbar round cell sarcoma in a 10-week-old rottweiler puppy.","authors":"Katrine Vestergaard Kristiansen, Anders Simon Schrøder, Dorothee Bienzle, Tanja Vedel, Jørgen Steen Agerholm, Mette Berendt","doi":"10.1186/s13028-025-00800-1","DOIUrl":"10.1186/s13028-025-00800-1","url":null,"abstract":"<p><strong>Background: </strong>Spinal neoplasms are sparsely documented in juvenile dogs. Case reports and small case series have described nephroblastomas, primitive neuroectodermal tumours, gliomas, certain sarcomas, and osteochondromas, but round cell sarcomas have not previously been documented.</p><p><strong>Case presentation: </strong>This case report describes a 10-week-old female Rottweiler puppy with acute onset of progressive ataxia and pelvic limb lameness. Neurological examination localised a T3-L3 myelopathy and MRI revealed an ovoid, well-marginated mass extending from mid L3 to caudal L4 vertebrae. Post-mortem examination, histopathology, and immunohistochemistry confirmed a round cell sarcoma of extradural origin.</p><p><strong>Conclusion: </strong>Our case report stresses the importance of performing MRI even in very young individuals with acute progressive signs of spinal cord lesions. Clinicians should include spinal tumours as a differential diagnosis for juvenile canines with spinal neurological signs. Round cell sarcoma should be added to the list of spinal tumours in young dogs.</p>","PeriodicalId":7181,"journal":{"name":"Acta Veterinaria Scandinavica","volume":"67 1","pages":"12"},"PeriodicalIF":1.9,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905653/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}