微生物学报 Pub Date : 2020-06-08 DOI: 10.13343/j.cnki.wsxb.20200102

朱晓峰 | 张桢 | 崔雷鸿 | 朱伟云 | 杭苏琴

引用次数: 0

高效组成型分泌表达木素过氧化物酶酿酒酵母工程菌的构建高效组成型分泌表达木素过氧化物酶酿酒酵母工程菌的构建

微生物学报 Pub Date : 2020-01-21 DOI: 10.13343/j.cnki.wsxb.20190373

肖建龙 | 张斯童 | 孙晓仲 | 陈光

引用次数: 0

有氧条件下污染禾谷镰刀菌的玉米品质变化规律和呕吐毒素的积累动态变化规律有氧条件下污染禾谷镰刀菌的玉米品质变化规律和呕吐毒素的积累动态变化规律

微生物学报 Pub Date : 2020-01-17 DOI: 10.13343/j.cnki.wsxb.20190514

闫珊珊 | 刘光芒 | 王建萍 | 林燕 | 白世平 | 张克英 | 吴彩梅

引用次数: 0

[Antibacterial activity of silver nanoparticles against multiple drug resistant strains]. [银纳米颗粒对多种耐药菌株的抑菌活性]。

微生物学报 Pub Date : 2017-04-04

Xueqing Chen, Jiaxuan Jiang, Zhihong Ren, Juan Li, Hongying Zhang, Jianguo Xu, Huamao Du

{"title":"[Antibacterial activity of silver nanoparticles against multiple drug resistant strains].","authors":"Xueqing Chen, Jiaxuan Jiang, Zhihong Ren, Juan Li, Hongying Zhang, Jianguo Xu, Huamao Du","doi":"","DOIUrl":"","url":null,"abstract":"Objective: The objective of the study was to assess the antimicrobial activity of silver nanoparticles (AgNPs) against multiple drug resistant strains.Methods: Minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgNPs against three model microbes, namely Escherichia coli, Staphylococcus aureus, Candida albicans were measured by microdilution broth method. Time-kill curve within 24 h was made according to colony count method after three model microbes were treated with a series concentration of AgNPs. Post-antibiotic effect was tested by colony count method. Finally, we determined the antimicrobial efficacy against multiple drug resistant strains in biological safety laboratory grade 2 (BSL-2).Results: AgNPs with a diameter of 5 nm to 30 nm were synthesized by the biological method. The zeta potential was -19.5 mV. The time-kill curve of the three model microbes showed time-dependent antibacterial activity. The effect of AgNPs on E. coli and C. albicans after \"antibiotic effect\" increased with time, there was no obvious \"post-antibiotic effect\" on S. aureus. Both MIC values and MBC values of AgNPs for the three model microbes were between 1 μg/mL and 4 μg/mL. However, the MIC value of AgNPs for the three human multidrug-resistant strains was 6 μg/mL to 26 μg/mL and MBC value of AgNPs was 10 μg/mL to 32 μg/mL. The MIC values of AgNPs for 14 animal multi-drug resistant strains were between 4 μg/mL and 10 μg/mL, and the MBC values were between 8 μg/mL and 16 μg/mL. The MBC/MIC values of all the tested strains were less than 2.Conclusion: AgNPs is a time-dependent antimicrobial agent with different \"post-antibiotic effect\", which can inhibit both human and animal-derived multi-drug resistant bacteria.","PeriodicalId":7120,"journal":{"name":"微生物学报","volume":"57 4","pages":"539-49"},"PeriodicalIF":0.0,"publicationDate":"2017-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36095595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Distribution, structure and sequence alignment, and metagenomics analysis of two nitrite reductases with NO forming]. [两种亚硝酸盐还原酶的分布、结构和序列比对及宏基因组学分析]。

微生物学报 Pub Date : 2017-04-04

Yufeng Xin, Tianying Zhao, Xiaohua Qu

{"title":"[Distribution, structure and sequence alignment, and metagenomics analysis of two nitrite reductases with NO forming].","authors":"Yufeng Xin, Tianying Zhao, Xiaohua Qu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To reflect the importance of nitrite reductase (NIR) in the environment, we studied its distribution.Methods: The sequences of NIR were searched in the sequenced genome database at NCBI based on previous reported NIR sequences. The sequence similarity was done by multiple sequence alignment, and phylogenetic relationship was evaluated via constructing the phylogenetic tree. Furthermore, their distribution in the marine metagenome was studied by metagenomics.Results: The homologues of these two enzymes were 397 and 812 strains in sequenced genome, and the proportion was 8 and 15.7 percent, respectively. Almost all of archaea containing type II NIR. They have high identity by multiple sequence alignment analysis. The cofactor, the substrate and the cooper binding sites in type II were high conserved, suggesting that these enzymes had the specific function in denitrification. Phylogenetic analysis showed the two enzymes may have the common ancestor. In marine metagenome analysis, type I and II have 6 and 35 reads per 100000 reads, respectively, the two types of NIRs have the biggest proportion at the tropical south pacific area.Conclusion: Collectively, we suggested NIR, especially type II, play a key role in bioremediation of nitrogen contamination.","PeriodicalId":7120,"journal":{"name":"微生物学报","volume":"57 4","pages":"597-608"},"PeriodicalIF":0.0,"publicationDate":"2017-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36095600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Phylogenetic and genetic heterogeneity of 23 Acidithiobacillus strains isolated from different geographical locations]. [不同地理位置分离的23株酸性硫杆菌的系统发育和遗传异质性]。

微生物学报 Pub Date : 2017-04-04

Pengwu Lin, Minrui Liu, Min Zhang, Aiying Wang, Yongqing Ni

{"title":"[Phylogenetic and genetic heterogeneity of 23 Acidithiobacillus strains isolated from different geographical locations].","authors":"Pengwu Lin, Minrui Liu, Min Zhang, Aiying Wang, Yongqing Ni","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To study the phylogenetic and genetic heterogeneity of 23 Acidithiobacillus strains from various geographical locations, as well as the relationship between the DNA fingerprinting classification and geographical origin of Acidithiobacillus.Methods: Partial 16S-23S rRNA gene intergenic spacer (ITS) was used to construct corresponding phylogenetic trees based on the sequence homology. rus gene amplification and rep-PCR assay with two different primers (BOXAIR and ERIC) were performed to analyze genetic heterogeneity of Acidithiobacillus strains from diverse environment.Results: Acidithiobacillus revealed a great genetic heterogeneity. The whole isolates were classified into five groups by ITS sequence analysis. This result was similar with that obtained by rep-PCR. Acidithiobacillus ferrooxidans strains were always divided into two groups of phylogenetic and BOXAIR fingerprinting cluster analysis. However, these were clustered one group in the ERIC dendrogram. Genotypic analysis of the rus gene suggested that different iron oxidation pathways have been evolved in these closely related bacteria. Taken together, the iron oxidation pathway of Acidithiobacillus and phylogenetic groups have no obvious correlation. ITS gene has been proven very useful in distinguishing closely related species or subspecies of Acidithiobacillus, to BOXAIR-PCR, which has been recommended as reliable tool for genetic heterogeneity analysis of Acidithiobacillus.","PeriodicalId":7120,"journal":{"name":"微生物学报","volume":"57 4","pages":"560-70"},"PeriodicalIF":0.0,"publicationDate":"2017-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36095597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Diversity of microbial community structure in the spermosphere of saline-alkali soil in shandong area]. [山东地区盐碱地土壤中微生物群落结构多样性]。

微生物学报 Pub Date : 2017-04-04

Zhimeng Zhang, Dunwei Ci, Guanchu Zhang, Hong Ding, Jishun Yang, Liangxiang Dai, Dai Zhang

{"title":"[Diversity of microbial community structure in the spermosphere of saline-alkali soil in shandong area].","authors":"Zhimeng Zhang, Dunwei Ci, Guanchu Zhang, Hong Ding, Jishun Yang, Liangxiang Dai, Dai Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Three soil types in different salt contents were taken as the experiment objectives. We evaluated the effect of various saline alkali soil types on diversity of bacterial community structure in spermosphere soil during water absorption and germination of peanut seeds.Methods: The V3-V4 region of 16S ribosomal RNA genes was amplified using PCR, and the PCR products were then analyzed using Illumina high-throughput sequencing technology.Results: (1) The diversity of soil bacterial community in saline alkali soil was higher than that in non-saline alkali soil. Especially, the highest diversity was in spermosphere soil from Qingtuo. (2) The microflora structures in different soils were distinct at the class level. Soil bacteria in four samples were classified into six classes, including Proteobacteria, Actinobacteria, Actinobacteria, Bacteroidetes, Acidobacteria and Firmicutes. Proteobacteria and Actinobacteria groups were dominant in colonies. The analysis of whole samples colony structure showed that the difference of type and abundance at phylum and genus level during different adsorption time was most significant (P<0.05). (3) The analysis of beta diversity and phylogenetic distances of constructed phylogenetic trees revealed that the sequenced clones fell into two major groups within the domain bacteria.Conclusion: The diversity of bacteria community compositions in the high salt content soil was higher. There were obvious differences in microbial community structure of different soil types at class level, primarily in the Proteobacteria and Actinobacteria. The type and abundance of microbial colonies at both phylum and genus levels were affected by the seed germination time. However, there was no influence on the genetic distance between the samples from the same soil type.","PeriodicalId":7120,"journal":{"name":"微生物学报","volume":"57 4","pages":"582-96"},"PeriodicalIF":0.0,"publicationDate":"2017-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36095599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Putative regulatory protein STM14_3514 decreases Salmonella Typhimurium invasion of epithelial cells]. [推测的调节蛋白STM14_3514减少鼠伤寒沙门氏菌侵袭上皮细胞]。

微生物学报 Pub Date : 2017-04-04

Lingyan Jiang, Qixing Zhou, Peisheng Wang, Xiaohan Jiang, Lu Feng

{"title":"[Putative regulatory protein STM14_3514 decreases Salmonella Typhimurium invasion of epithelial cells].","authors":"Lingyan Jiang, Qixing Zhou, Peisheng Wang, Xiaohan Jiang, Lu Feng","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To study the function and mechanism of STM14_3514 gene that encoded in Salmonella pathogenicity island (SPI)-1 of Salmonella enterica serovar Typhimurium strain ATCC 14028.Methods: We constructed STM14_3514 mutant strain and a complemented strain of the mutant. Through mice experiment, attachment assays, invasion assays, macrophage replication assays, western blot, and Quantitative real-time PCR analysis (qRT-PCR), we compared the virulence of the mutant strain to that of the wild-type 14028.Results: STM14_3514 mutant shows increased virulence to mice, and the bacterial number of STM14_3514 mutant in liver, spleen, and ileum was more abundant than that of the wild-type strain. The increased virulence of STM14_3514 mutant is caused by its elevated invasion ability to epithelial cells (>2-fold and P<0.05). qRT-PCR and western blot results show that STM14_3514 reduced the expression of HilA and another SPI-1invasion locus. Moreover, the repression of HilA by STM14_3514 is mediated by HilC.Conclusion: STM14_3514 is a negative regulator in SPI-1, which can repress HilA and SPI-1invasion locus through HilC, and possibly contribute to the repression on SPI-1 after bacterial invasion.","PeriodicalId":7120,"journal":{"name":"微生物学报","volume":"57 4","pages":"500-12"},"PeriodicalIF":0.0,"publicationDate":"2017-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36095181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Two sample pooling strategies revealed different root-associated fungal diversity of Rhododendron species]. [两种样本池策略揭示了不同杜鹃花物种根相关真菌多样性]。

微生物学报 Pub Date : 2017-04-04

Caiwei Huang, Yinghui Liao, Qiong Ding

{"title":"[Two sample pooling strategies revealed different root-associated fungal diversity of Rhododendron species].","authors":"Caiwei Huang, Yinghui Liao, Qiong Ding","doi":"","DOIUrl":"","url":null,"abstract":"Objective: ooling of multiple samples is widely used in studying general patterns of microbial communities that are heterogeneously structured in space. Pooling strategies and the number of sequence reads generate biases in the description of diversity and community structure of root-associated fungi. Therefore, we developed a molecular toolbox for fast and accurate identification of the root-associated fungal community of Rododendron species.Methods: Multiple root samples of R. lutescens and R. bureavii were collected for DNA extraction. Effects of two different pooling strategies, i.e. pooling samples prior to vs. post PCR, on fungal species composition were studied by comparing results within host species.Results: Species richness and Shannon-Wiener index of fungal communities of clone library constructed by pooling samples after PCR were higher than that of pooling prior to PCR. High frequency fungal species were detected by both pooling strategies, whereas infrequent species detected by the two strategies differed. Notably, the prior to PCR pooling strategy effectively alleviated the unwanted amplification of host plant sequences when fungal specific primer ITS1f and ITS4 were used. Accumulation curves of fungal species suggested that sequencing at least 50 clones can fully reflect species composition of clone library of the two Rhododendron root-associated fungal community.Conclusion: Clone library constructed by post PCR pooling of samples is better in providing accurate views of fungal diversity and community structure of Rhododendron species.","PeriodicalId":7120,"journal":{"name":"微生物学报","volume":"57 4","pages":"571-81"},"PeriodicalIF":0.0,"publicationDate":"2017-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36095598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effects of glucose on photosynthesis and growth of Chloralla sp. HN08 cells]. 葡萄糖对小藻HN08细胞光合作用和生长的影响[j]。

微生物学报 Pub Date : 2017-04-04

Xiaoyu Lang, Zhiyuan Liu, Meng Xu, Lingyu Xie, Rongzhen Li

{"title":"[Effects of glucose on photosynthesis and growth of Chloralla sp. HN08 cells].","authors":"Xiaoyu Lang, Zhiyuan Liu, Meng Xu, Lingyu Xie, Rongzhen Li","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To study the potential of using glucose as carbon source to produce microalgae biomass and biochemical components, such as photosynthetic pigments, lipids, carbohydrates and proteins by tropical marine microalgae Chloralla sp. HN08.Methods: We compared the growth characteristics of Chloralla sp. HN08 cells under photoautotrophic and mixotrophic (10 g/L glucose was added into the medium) conditions. The photosynthesis, specific growth rates, cell densities, and the content of cell's major components including lipids, starch, soluble sugar, and soluble protein were determined and compared.Results: Glucose (10 g/L in medium) could promote Chlorella growth and increase the final cell density under light condition. However, cells declined gradually under heterotrophic condition. Under mixotrophic condition, the specific growth rate and the final cell density were 6.8 and 1.3 times as that of cells under photoautotrophic condition, respectively. The content of soluble sugar, starch, and lipids in mixotrophic cells was also significantly higher (P<0.05) than that in photoautotrophic cells. However, the content of soluble protein and photosynthetic pigments of mixotrophic cells was significantly lower (P<0.05) than that of autotrophic cells. Algae culture with glucose addition showed a higher light saturation as well as respiration rate. No significant difference in net photosynthesis rate was found between autotrophic and mixotrophic cultures (P>0.05).Conclusion: Under light condition, glucose as a carbon source can promote lipids and starch accumulation, as well as biomass production.","PeriodicalId":7120,"journal":{"name":"微生物学报","volume":"57 4","pages":"550-9"},"PeriodicalIF":0.0,"publicationDate":"2017-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36095596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

微生物学报最新文献