Acta Leidensia最新文献

Ultrastructural changes in the blood-brain barrier of mice infected with Plasmodium berghei. 感染伯氏疟原虫小鼠血脑屏障超微结构的改变。

Acta Leidensia Pub Date : 1992-01-01

T W Polder, W M Eling, J H Curfs, C R Jerusalem, M Wijers-Rouw

{"title":"Ultrastructural changes in the blood-brain barrier of mice infected with Plasmodium berghei.","authors":"T W Polder, W M Eling, J H Curfs, C R Jerusalem, M Wijers-Rouw","doi":"","DOIUrl":"","url":null,"abstract":"Mice infected with the rodent malaria parasite Plasmodium berghei exhibit ultrastructural changes of the blood-brain barrier during the course of infection. Firm adherence including cellular interdigitation of infected cells or leucocytes and even clusters of cells to the vascular-endothelial lining is repeatedly observed early during infection. Ghosts and membrane remnants can be found engulfed in the surface of the endothelial cells. Frequently leucocytes migrate between endothelial cells and even cause a lift off and degeneration of these cells. In addition, endothelial cells exhibit increased pinocytotic activity, many irregular cytoplasmic extensions and even phagocytic activity. These changes are associated with degenerative changes in the basement membrane. Swelling and deposition of collagen-like fibres and even loss of fragments of basement membrane is observed. In some places fingerlike extensions of pericytes passed through the basement membrane and contacted or even bulged into the cytoplasm of endothelial cells. Ballooning and even coalescence of perivascular astrocytes was observed and contributed to the appearance of a perivascular oedematous space. The observed changes indicate a progressive deterioration of the blood-brain barrier eventually leading to endothelial lesions and hemorrhage.","PeriodicalId":7108,"journal":{"name":"Acta Leidensia","volume":"60 2","pages":"31-46"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12654772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Onchocerciasis in the forest-savannah mosaic region of Sierra Leone. Prevalence, intensity of infection, and endemicity levels. 塞拉利昂森林草原马赛克地区的盘尾丝虫病。流行程度、感染强度和地方性水平。

Acta Leidensia Pub Date : 1992-01-01

H R Sesay, M Trpis

{"title":"Onchocerciasis in the forest-savannah mosaic region of Sierra Leone. Prevalence, intensity of infection, and endemicity levels.","authors":"H R Sesay, M Trpis","doi":"","DOIUrl":"","url":null,"abstract":"The present study was carried out between December 1986 and January 1988. A series of 3,048 individuals (46% males and 54% females) were examined in 56 villages; onchocercal dermatitis and lymphadenitis were seen. The crude prevalence of nodules and of microfilariae amounted to 14% and 43%, respectively. The prevalence of nodules was significantly higher at the femoral trochanter than at other anatomical sites. The overall prevalence of nodules, and of microfilariae, was significantly higher in males than in females. The age-adjusted prevalence and the geometric mean microfilarial load were significantly higher in the cohort of males older than 15 years than in their female counterparts. The overall geometric mean microfilarial load was 18 microfilariae per skin snip. The correlation between prevalence and intensity of infection was poor, but was perfect between mean microfilarial density per skin snip and mean microfilarial density per milligram skin. The ratio of mean microfilarial density per skin snip to mean microfilarial density per milligram skin was 1:6. The correlation between prevalence of onchocercomata and microfilarial prevalence or microfilarial density was weak. Both of the latter criteria identified the Rokel/Seli as the river with the highest proportion of hyperendemic villages. The implications of the present findings for the epidemiology of onchocerciasis are discussed.","PeriodicalId":7108,"journal":{"name":"Acta Leidensia","volume":"60 2","pages":"61-77"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12655298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A small yaws survey on the island of Sumatra, Indonesia. 印度尼西亚苏门答腊岛上的小型雅司病调查。

Acta Leidensia Pub Date : 1992-01-01

H J Engelkens, E Stolz

引用次数: 0

Tick-borne encephalitis in Europe. 欧洲的蜱传脑炎。

Acta Leidensia Pub Date : 1992-01-01

C Kunz

引用次数: 0

Serologic evidence for tick-borne encephalitis (TBE) in North-American military stationed in Germany. 驻德北美军队蜱传脑炎(TBE)的血清学证据。

Acta Leidensia Pub Date : 1992-01-01

J Clement, H Leirs, V Armour, D Ward, J Groen, A Osterhaus, C Kunz

引用次数: 0

Typing leptospira from the perspective of a reference laboratory. 从参比实验室的角度分型钩端螺旋体。

Acta Leidensia Pub Date : 1992-01-01

W J Terpstra

{"title":"Typing leptospira from the perspective of a reference laboratory.","authors":"W J Terpstra","doi":"","DOIUrl":"","url":null,"abstract":"Leptospirosis is caused by different leptospiral variants. Analysis by cross agglutination absorption tests (CAAT) led to the definition of entities called serovars to distinguish between leptospires on sub-species level, and to the designation of reference strains representing serovars. For decades CAAT has been used to classify leptospires and now approximately 200 serovars have been recognized. In the last few years, it has become increasingly more clear that the serovar concept is no longer fully satisfactory as it may fail to adequately define epidemiologically important entities. In addition, CAAT is too cumbersome and time-consuming for routine typing. Various methods have been developed based on antigenic or genetic analysis with the purpose to supplement or to replace the CAAT. Most of these methods are still in an experimental state. It is to be expected that a typing method based on genomic analysis will eventually become most important. Such a new method should have considerable advantages in order to be acceptable for the development of a new classification system replacing the system based on serovars, which is widely accepted and in many respects still satisfactory. From the new methods, analysis of leptospiral DNA fragment length after digestion with restriction enzymes (REA) has been widely used and proven to be useful for typing. Pending the development of new typing methods that have clear advantages and may lead to a new classification system, it is suggested that the classification system based on serovars is maintained and that REA is added to each description of a new serovar.","PeriodicalId":7108,"journal":{"name":"Acta Leidensia","volume":"60 2","pages":"79-87"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12655299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Onchocerca Volvulus infection in Sierra Leone: relation between prevalence, intensity of infection, and ocular problems in a 'forest' region. 塞拉利昂盘尾丝虫感染:“森林”地区流行率、感染强度与眼部问题之间的关系

Acta Leidensia Pub Date : 1992-01-01

A A Gbakima, R F Barbe

引用次数: 0

Stage specificity of expression malaria antigens. 疟疾抗原表达的阶段特异性。

Acta Leidensia Pub Date : 1991-01-01

B Sina, M R Hollingdale

引用次数: 0

Conservation and divergence of repeated structures in Plasmodium genomes: the molecular drift. 疟原虫基因组重复结构的保存和分化:分子漂移。

Acta Leidensia Pub Date : 1991-01-01

C Frontali, E Pizzi