Acta Biotechnologica最新文献

The effect of rhizodeposition from helophytes on bacterial degradation of phenolic compounds 植物根沉积对细菌降解酚类化合物的影响

Acta Biotechnologica Pub Date : 2002-05-01 DOI: 10.1002/1521-3846(200205)22:1/2<107::AID-ABIO107>3.0.CO;2-#

H. Moormann, P. Kuschk, U. Stottmeister

{"title":"The effect of rhizodeposition from helophytes on bacterial degradation of phenolic compounds","authors":"H. Moormann, P. Kuschk, U. Stottmeister","doi":"10.1002/1521-3846(200205)22:1/2<107::AID-ABIO107>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/1521-3846(200205)22:1/2<107::AID-ABIO107>3.0.CO;2-#","url":null,"abstract":"The effect of rhizodeposition from helophytes (aquatic plants) on the bacterial degradation of toxic compounds such as phenolic substances was tested. Investigations were carried out as batch experiments with rhizodeposition products obtained from helophytes. DOC concentrations, which were used as points of reference for rhizodeposition, were between 2.5 and 12 mg per litre. Rhizodeposition was found to advance the biodegradation of 4-chlorophenol when using a mixed bacterial culture and pure cultures of bacteria previously isolated from Phalaris arundinacea roots. This stimulation is a result of rhizodeposition products serving as growth substrates for the bacteria. Investigations with Ralstonia eutropha (DSMZ Braunschweig, strain 5536) confirmed the function of rhizodeposition products as growth substrates. Degradation by Acinetobacter baumannii and Ralstonia sp. obtained from Phalaris arundinacea was'accompanied by the dechlorination of 4-chlorophenol. There was no enhancing impact on the degradation of the substances phenol and 2,6-dimethylphenol by rhizodeposition products.","PeriodicalId":7037,"journal":{"name":"Acta Biotechnologica","volume":"71 1","pages":"107-112"},"PeriodicalIF":0.0,"publicationDate":"2002-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75656058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Exposure to xenobiotics deeply affects the bacteriocenosis in the rhizosphere of helophytes 暴露于外来生物对植生植物根际的细菌中毒有深刻的影响

Acta Biotechnologica Pub Date : 2002-05-01 DOI: 10.1002/1521-3846(200205)22:1/2<161::AID-ABIO161>3.0.CO;2-#

U. Soltmann, H. Wand, A. Müller, P. Kuschk, U. Stottmeister

{"title":"Exposure to xenobiotics deeply affects the bacteriocenosis in the rhizosphere of helophytes","authors":"U. Soltmann, H. Wand, A. Müller, P. Kuschk, U. Stottmeister","doi":"10.1002/1521-3846(200205)22:1/2<161::AID-ABIO161>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/1521-3846(200205)22:1/2<161::AID-ABIO161>3.0.CO;2-#","url":null,"abstract":"Upon exposure to the aromatic xenobiotics 2,6-dimethylphenol and 4-nitrophenol, distinct changes occurred in the microbial structure of the rhizosphere of the helophytes Carex graclis, Phalaris arundinacea and Juncus effusus (total number, diversity, abundance). Generally speaking, exposure to xenobiotics resulted in an increase in the total numbers of xenobiotic-degrading bacteria on roots and sand particles. The number of bacteria per cm 2 was significantly higher on the root surface than on the surface of sand particles in the rhizosphere. PCR fingerprinting methods such as RAPD (Randomly Amplified Polymorphic DNA) and 16S-23S rDNA intergenic spacer amplification or alternatively rRNA-targeted gene probes for the α-, β-, γ-subclasses of Proteobacteria, the Xanthomonas branch of Proteobacteria and Actinobacteria, were used to determine the diversity of arbitrary samples of bacterial isolates. The diversity of bacteria isolated from the rhizosphere decreased depending on the exposure time. The degrading bacteria were dominated by GRAM-positives. In the case of exposure to 2,6-dimethylphenol of Carex gracilis, GRAM-positive isolates became more dominant as the exposure time increased. Thus it was that after more than six months exposure to 2,6-dimethylphenol, only the GRAM-positives were isolated from a sand-bed reactor planted with Carex gracilis. Some of these isolates show interesting properties in addition to a high 2,6-dimethylphenol degradation rate. The 2,6-dimethylphenol degrading Mycobacterium sp. DMP 20 represents the first fluorescing GRAM-positive bacterium so far described. The introduction of allochthonous, high-performance degraders resulted in Mycobacterium sp. DMP 20 being permanently established in the rhizosphere, whereas the naphthalene degrader Pseudomonas putida PpG7 did not remain in the system.","PeriodicalId":7037,"journal":{"name":"Acta Biotechnologica","volume":"7 1","pages":"161-166"},"PeriodicalIF":0.0,"publicationDate":"2002-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79475414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Production of Indole Acetic Acid in Culture by a Rhizobium Species from the Root Nodules of a Monocotyledonous Tree, Roystonea regia 一种根瘤菌在单子叶树根瘤培养中生产吲哚乙酸的研究

Acta Biotechnologica Pub Date : 2001-02-01 DOI: 10.1002/1521-3846(200102)21:1<65::AID-ABIO65>3.0.CO;2-#

P. S. Basu, A. C. Ghosh

引用次数: 34

Gentechnik, Biotechnik. Stuttgart: Wissenschaftliche Verlagsgesellschaft mbH, 632 pages, 53 tables, 500 figures; DM 168.00 ISBN 3‐8047‐1597‐4

Acta Biotechnologica Pub Date : 2000-01-01 DOI: 10.1002/abio.370200304

B. Kiesel

引用次数: 0

Chemical sensors and biosensors for medical and biological applications. Chichester, New York, Weinheim, Brisbane, Singapore, Toronto: John Wiley & Sons XII + 413 pages, 82 figures, 41 tables; DM 198.00, ISBN 3-527-28855-4 医学和生物应用的化学传感器和生物传感器。奇切斯特，纽约，Weinheim，布里斯班，新加坡，多伦多:John Wiley & Sons XII + 413页，82张图，41张表;Dm 198.00, isbn 3-527-28855-4

Acta Biotechnologica Pub Date : 2000-01-01 DOI: 10.1002/abio.370200307

H. Schmauder

引用次数: 2

Socio‐ecological strategies for future sustainability a review of an internet conference 未来可持续发展的社会生态策略——互联网会议综述

Acta Biotechnologica Pub Date : 2000-01-01 DOI: 10.1002/ABIO.370200305

H. Doelle, E. Foo

{"title":"Socio‐ecological strategies for future sustainability a review of an internet conference","authors":"H. Doelle, E. Foo","doi":"10.1002/ABIO.370200305","DOIUrl":"https://doi.org/10.1002/ABIO.370200305","url":null,"abstract":"The recent upsurge in information technology has provided the international community with an easy access to professional journals (e.g. Electronic Journal of Biotechnology at http://www.ejb.org; etc.), discussion groups (e.g. bioenergy@crest.org; digestion@crest.org; etc.) and recently to electronic international conferences (e.g. ICIBS; http://www.cid.harvard.edu/cidbiotech, etc.) as well as a series of biotechnological information material (e.g. http://www.psrast.org, etc.) to stay in contact and receive up-to-date information in biotechnology. There is no doubt that this new technology will be more cost effective in future and reach more people in communities around the globe. This review reports on one such an electronic conference aiming at bridging the communication gap between developed and developing countries. This conference dealt with integrated biosystems and has provided an excellent forum for more than 100 active participants from all regions of the world. As has been demonstrated in this review, the conference was able to show the very different approaches towards the use of biotechnology in developed and developing countries, cold and tropical climate regions owing to their different ecological, economical and societal problems. It also demonstrated very clearly that the field of molecular genetics and/or genetic engineering is not a priority issue in developing countries, but rather the need for clean technologies, multiproduct formation through socio-economic integrated biosystems, e.g. incorporating microbial waste management into agro-industries, in human activities and their roles in creating better health conditions, a better environment and sustain development. It is hoped that this review will lead to a greater use of the electronic facilities available to inform and educate both the northern and the southern communities more readily of their needs and requirements to improve understanding and efforts for a sustainable future.","PeriodicalId":7037,"journal":{"name":"Acta Biotechnologica","volume":"359 1","pages":"203-218"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84892926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Reduction of halogenated derivatives of benzoic acid to the corresponding alcohols by Desulfovibrio vulgaris PY1 寻常脱硫弧菌PY1还原苯甲酸卤化衍生物为相应醇

Acta Biotechnologica Pub Date : 2000-01-01 DOI: 10.1002/ABIO.370200303

M. Bock, H. Kneifel, S. Schoberth, H. Sahm

{"title":"Reduction of halogenated derivatives of benzoic acid to the corresponding alcohols by Desulfovibrio vulgaris PY1","authors":"M. Bock, H. Kneifel, S. Schoberth, H. Sahm","doi":"10.1002/ABIO.370200303","DOIUrl":"https://doi.org/10.1002/ABIO.370200303","url":null,"abstract":"Desulfovibrio vulgaris strain PY1 was isolated from a 3-chlorobenzoic acid (3CBA) degrading anaerobic enrichment culture, using anaerobic Percoll density centrifugation. When grown on pyruvate (20 mM), in the absence of sulphate and under strict anaerobic conditions, this organism converted not only the co-substrates benzoate (BA), 3-amino-BA and 3CBA to the corresponding alcohols but also ten other different halogenated benzoic acids, viz., 4-Cl-, 3-Br-, 4-Br-, 3-I-, 3-F-, 4-F-, 2,4-di-Cl-, 2,5-di-CI-, 3,4-di-Cl- and 3,5-di-Cl-BA. This was verified with HPLC and GC/MS spectrometric analyses. The yields of the co-substrate converted after 30 days of growth were between 20% and 88%, depending on the compounds which had been added at initial concentrations of 500 μM. Sulphate, sulphite, thiosulphate and disulphite inhibited the formation of 3-Cl-benzyl alcohol (3CBOH), i.e. a 97 to 99% inhibition, and nitrate and sulphur had no effect (a 7-10% inhibition). In cell-free extracts, the reduction of 3CBA to 3CBOH required strict anaerobic conditions, pyruvate or H 2 as electron donors and the addition of methylviologen (MV), FAD, FMN or ferre-doxin as electron carriers. The specific activity of the reduction of 3CBA to 3CBOH in crude extract was 5.3 nmol/(mg protein min). The reaction was not inhibited by additions of sulphate or sulphite (5 mM), but was completely inhibited at concentrations of 10 mM 3CBA or 50 mM BA. A carboxylic acid reductase (aldehyde dehydrogenase), which acted on non-activated 3CBA and was responsible for the reduction of 3CBA to 3-Cl-benzaldehyde, was found in the soluble fraction (94% of the total activity). These results demonstrate that strain PY1 was able to effectively reduce a wide range of halogenated benzoic acids to the corresponding alcohols.","PeriodicalId":7037,"journal":{"name":"Acta Biotechnologica","volume":"160 1","pages":"189-201"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75682857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Flow cytometric monitoring of Rhodococcus erythropolis and Ochrobactrum anthropi in a mixed culture 混合培养中红红球菌和人类赭杆菌的流式细胞术监测

Acta Biotechnologica Pub Date : 2000-01-01 DOI: 10.1002/ABIO.370200306

S. Müler, A. Lösche, H. Mertingk, W. Beisker, W. Babel

{"title":"Flow cytometric monitoring of Rhodococcus erythropolis and Ochrobactrum anthropi in a mixed culture","authors":"S. Müler, A. Lösche, H. Mertingk, W. Beisker, W. Babel","doi":"10.1002/ABIO.370200306","DOIUrl":"https://doi.org/10.1002/ABIO.370200306","url":null,"abstract":"The GRAM-positive bacterium Rhodococcus erythropolis K2-3 and the GRAM-negative Ochrobactrum anthropi K2-14 are capable of synergistically degrading 4-(2,4-dichlorophenoxy)butyric acid (2,4-DB). The two strains execute this task in a symbiotic manner, but the nature of the interactions involved in the degradation is only partially understood as yet. An essential first step in elucidating the interaction is to be able to monitor the two strains separately, at the cellular level, within mixed populations. Therefore a method exploiting fluorescently labelled lectin probes was developed. Since Concanavalin A (Con A) binds specifically to R. erythropolis K2-3, it was selected and linked to the fluorescent dye Bodipy 630/650, which has an excitation maximum in the red part of the visible light spectrum. Forward light scatter (FSC) and DNA fluorescence from both strains were also measured to obtain simultaneous information about their physiological states. The three parameters were conveniently monitored by dual and triple excitation flow cytometry in conjunction with double fluorescent staining techniques. In addition, the strains were identified using an epifluorescence microscope. These techniques were found powerful tools for the population analysis of this mixed bacterial system.","PeriodicalId":7037,"journal":{"name":"Acta Biotechnologica","volume":"22 1","pages":"219-233"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75095167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Formation of aminium lactates in lactic acid fermentation. Fermentative production of 1,4‐piperazinium‐(L,L)‐dilactate and its use as a starting material for the synthesis of dilactide (Part 2) 乳酸发酵过程中乳酸胺的形成。1,4‐哌嗪‐(L,L)‐苯二甲酸酯的发酵生产及其作为苯二甲酸酯合成原料的应用(第二部分)

Acta Biotechnologica Pub Date : 2000-01-01 DOI: 10.1002/ABIO.370200310

B. Kamm, M. Kamm, K. Richter, W. Reimann, A. Siebert

{"title":"Formation of aminium lactates in lactic acid fermentation. Fermentative production of 1,4‐piperazinium‐(L,L)‐dilactate and its use as a starting material for the synthesis of dilactide (Part 2)","authors":"B. Kamm, M. Kamm, K. Richter, W. Reimann, A. Siebert","doi":"10.1002/ABIO.370200310","DOIUrl":"https://doi.org/10.1002/ABIO.370200310","url":null,"abstract":"A fermentation process for manufacturing 1,4-piperazinium-(L,L)-dilactate from renewable raw materials and a method for processing this product into L,L-dilactide are described. Lactic acid fermentation with Lactobacillus paracasei was modified in such a way that pH control occurred by using an aqueous solution of piperazine as a correcting agent instead of sodium hydroxide solution. The production of a stoichiometrically composed piperazinium lactate was possible when the pH was 5.0. From 5.0 kg of glucose and 2.15 kg of piperazine, 6.65 kg of 1,4-piperazinium-(L,L)-dilactate were formed in the fermentation process. Separation from fermentation broth, purification and concentration of the product in aqueous solutions were carried out by means of ultrafiltration, nanofiltration and electrodialysis. Total product retention by the membranes used was about 33%. The crystalline salt was obtained by vacuum evaporation. Processing of the 1,4- piperazinium-(L,L)-dilactate into L,L-dilactide was performed in a special glass reactor. A product yield of 70% was achieved. The purified product was characterized by elementary analysis, as well as solubility behaviour, polarity and spectroscopic data. An overall process consisting of the stages fermentation, purification and concentration of piperazinium dilactate as well as cyclization of the latter to dilactide is described.","PeriodicalId":7037,"journal":{"name":"Acta Biotechnologica","volume":"1 1","pages":"289-304"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79923849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

A laboratory guide to RNA: Isolation, analysis, and synthesis. New York, Chichester, Brisbane, Toronto, Singapore: John Wiley & Sons, 1996, 445 pages, 72 figures, 13 tables, £ 45.00 ISBN 0-471-12536-9: Book Review RNA的实验室指南:分离、分析和合成。纽约，奇切斯特，布里斯班，多伦多，新加坡:John Wiley & Sons, 1996, 445页，72个数字，13个表格，45.00英镑ISBN 0-471-12536-9:书评

Acta Biotechnologica Pub Date : 1997-01-01 DOI: 10.1002/abio.370170314

C. Föllner

引用次数: 0