Acta odontologica latinoamericana : AOL最新文献

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Methodological considerations for a model of endodontic treatment in Wistar rats. Wistar大鼠牙髓治疗模型的方法学考虑。
Acta odontologica latinoamericana : AOL Pub Date : 2020-12-01 DOI: 10.54589/aol.33/3/153
Sharon R. Oyhanart, M. C. Canzobre
{"title":"Methodological considerations for a model of endodontic treatment in Wistar rats.","authors":"Sharon R. Oyhanart, M. C. Canzobre","doi":"10.54589/aol.33/3/153","DOIUrl":"https://doi.org/10.54589/aol.33/3/153","url":null,"abstract":"The use of correctly designed animal models is a fundamental step prior to clinical trials in humans. Although rats are easy to house and handle, and have molars that resemble those of humans, very few researchers use them as a model for root canal treatment, probably due to their small size and the lack of relevant data necessary to reproduce the model. Our aims were to describe the anatomic and histologic characteristics of the mandibular first molar of the Wistar rat and present a standardised model for its experimental endodontic treatment. Twenty female rats were used. The characteristics of the mesial and distal roots were described histologically and the quality of the results achieved following the treatment protocol presented herein was assessed by means of digital radiographs, micro- CT and histological sections. The age of 55 days was found to be the most adequate for performing this technique, but we consider the interval of 50 to 60 days to be suitable. Both canals are oval, although in opposite planes, and the furcating-facing walls present the minimum dentine thickness. It was essential to become familiar with these aspects in order to decide upon the most appropriate instrumentation and obturation techniques that would enable replication of this model in basic science research.","PeriodicalId":7033,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75654496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Self-reported habits regarding dental bur use and conditioning among academic and non-academic argentinian dentists. 学术和非学术阿根廷牙医自我报告的牙齿使用和调理习惯。
Hernan J Tartacovsky, Veronica A Ciparelli, Lucia J Horvath, Martin Garcia-Cuerva, Sebastian Tortoni, Maria E Iglesias
{"title":"Self-reported habits regarding dental bur use and conditioning among academic and non-academic argentinian dentists.","authors":"Hernan J Tartacovsky,&nbsp;Veronica A Ciparelli,&nbsp;Lucia J Horvath,&nbsp;Martin Garcia-Cuerva,&nbsp;Sebastian Tortoni,&nbsp;Maria E Iglesias","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The removal of tissues affected by caries lesions is one of the most frequent procedures in daily dental practice. The aim of this study was to collect information about the habits of members of the academic and non-academic dental community in Argentina regarding the use and conditioning of burs employed in the removal of dentin during the treatment of carious lesions. A 14- item questionnaire was prepared, and once validated in small groups, it was sent to three lists of dental subscribers through the computer services area of the School of Dentistry, University of Buenos Aires, Argentina (FOUBA). By the deadline for receiving responses, which was set as four weeks after the sending date, 294 replies were received. Results: percentage (95%CI): 47.93% (42.18-53.68%) of the respondents were faculty members, of whom 37.24% (31.68-42.81%) claimed to belong to FOUBA. Of the respondents, 26.90% (21.79-32.00%) reported being under 35 years old, 37.59% (32.01-43.16%) 36 to 45 years old, 22.07% (17.30-26.84%) 46 to 55 years and 11.38% (7.72-15.03%) over 55 years. Regarding bur type, 68.07% (62.31%-73.44%) claimed to use round burs to remove carious dentin and 27.72% (22.60%-23.31%) round + others. Regarding rotary speed, 22.07% (17.30-26.84%) use medium speed, 32.76% (27.36-38.16%) use high speed, and 32.76% (27.36-38.16%) use medium + super high. Only 17.93% (13.52- 22.35%) indicated that they know how to quantify the number of times they use their burs, while 50.34% (44.59-56.10%) of the respondents said that they use burs \"until they no longer cut\", 23.79% (18.89-28.69%) use them 1 to 5 times. 98.27% (96.77-99.77%) said they clean their burs once used. When the answers regarding the use of rubber dam were compared with area of specialization (Chi-square), it was found that faculty members mention the use of burs under rubber dam always or almost always 74.82% (67.60-82.04%) while outside the academic field, 51.01% (42.98-59.03%) of dentists say that they never or almost never do so (p <0.001). Conclusion: Most respondents state that they sterilize their rotary instruments in some way after use. About half of them do not keep track of the number of uses and use burs \"until they no longer cut\".</p>","PeriodicalId":7033,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25317766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Self-reported habits regarding dental bur use and conditioning among academic and non-academic argentinian dentists. 学术和非学术阿根廷牙医自我报告的牙齿使用和调理习惯。
Acta odontologica latinoamericana : AOL Pub Date : 2020-12-01 DOI: 10.54589/aol.33/3/195
Hernan J Tartacovsky, Verónica Ciparelli, L. Horvath, Martín García-Cuerva, Sebastian Tortoni, M. Iglesias
{"title":"Self-reported habits regarding dental bur use and conditioning among academic and non-academic argentinian dentists.","authors":"Hernan J Tartacovsky, Verónica Ciparelli, L. Horvath, Martín García-Cuerva, Sebastian Tortoni, M. Iglesias","doi":"10.54589/aol.33/3/195","DOIUrl":"https://doi.org/10.54589/aol.33/3/195","url":null,"abstract":"The removal of tissues affected by caries lesions is one of the most frequent procedures in daily dental practice. The aim of this study was to collect information about the habits of members of the academic and non-academic dental community in Argentina regarding the use and conditioning of burs employed in the removal of dentin during the treatment of carious lesions. A 14- item questionnaire was prepared, and once validated in small groups, it was sent to three lists of dental subscribers through the computer services area of the School of Dentistry, University of Buenos Aires, Argentina (FOUBA). By the deadline for receiving responses, which was set as four weeks after the sending date, 294 replies were received. Results: percentage (95%CI): 47.93% (42.18-53.68%) of the respondents were faculty members, of whom 37.24% (31.68-42.81%) claimed to belong to FOUBA. Of the respondents, 26.90% (21.79-32.00%) reported being under 35 years old, 37.59% (32.01-43.16%) 36 to 45 years old, 22.07% (17.30-26.84%) 46 to 55 years and 11.38% (7.72-15.03%) over 55 years. Regarding bur type, 68.07% (62.31%-73.44%) claimed to use round burs to remove carious dentin and 27.72% (22.60%-23.31%) round + others. Regarding rotary speed, 22.07% (17.30-26.84%) use medium speed, 32.76% (27.36-38.16%) use high speed, and 32.76% (27.36-38.16%) use medium + super high. Only 17.93% (13.52- 22.35%) indicated that they know how to quantify the number of times they use their burs, while 50.34% (44.59-56.10%) of the respondents said that they use burs \"until they no longer cut\", 23.79% (18.89-28.69%) use them 1 to 5 times. 98.27% (96.77-99.77%) said they clean their burs once used. When the answers regarding the use of rubber dam were compared with area of specialization (Chi-square), it was found that faculty members mention the use of burs under rubber dam always or almost always 74.82% (67.60-82.04%) while outside the academic field, 51.01% (42.98-59.03%) of dentists say that they never or almost never do so (p <0.001). Conclusion: Most respondents state that they sterilize their rotary instruments in some way after use. About half of them do not keep track of the number of uses and use burs \"until they no longer cut\".","PeriodicalId":7033,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77458064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Methodological considerations for a model of endodontic treatment in Wistar rats. Wistar大鼠牙髓治疗模型的方法学考虑。
Sharon R Oyhanart, Mariela C Canzobre
{"title":"Methodological considerations for a model of endodontic treatment in Wistar rats.","authors":"Sharon R Oyhanart,&nbsp;Mariela C Canzobre","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The use of correctly designed animal models is a fundamental step prior to clinical trials in humans. Although rats are easy to house and handle, and have molars that resemble those of humans, very few researchers use them as a model for root canal treatment, probably due to their small size and the lack of relevant data necessary to reproduce the model. Our aims were to describe the anatomic and histologic characteristics of the mandibular first molar of the Wistar rat and present a standardised model for its experimental endodontic treatment. Twenty female rats were used. The characteristics of the mesial and distal roots were described histologically and the quality of the results achieved following the treatment protocol presented herein was assessed by means of digital radiographs, micro- CT and histological sections. The age of 55 days was found to be the most adequate for performing this technique, but we consider the interval of 50 to 60 days to be suitable. Both canals are oval, although in opposite planes, and the furcating-facing walls present the minimum dentine thickness. It was essential to become familiar with these aspects in order to decide upon the most appropriate instrumentation and obturation techniques that would enable replication of this model in basic science research.</p>","PeriodicalId":7033,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25317391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cognitive impairment related to arterial stiffness in cardiovascular disease patients with severe periodontitis. 心血管疾病伴严重牙周炎患者与动脉僵硬相关的认知障碍
María Del C Rubio, Jesica J Rudzinski, Cecilia Ramos, Fima Lifshitz, Silvia M Friedman, Liliana N Nicolosi
{"title":"Cognitive impairment related to arterial stiffness in cardiovascular disease patients with severe periodontitis.","authors":"María Del C Rubio,&nbsp;Jesica J Rudzinski,&nbsp;Cecilia Ramos,&nbsp;Fima Lifshitz,&nbsp;Silvia M Friedman,&nbsp;Liliana N Nicolosi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Severe periodontal disease (SPD) associated with systemic peripheral inflammation, cognitive impairment (CI) and arterial stiffness (AS) has been recognized. The aim of this study was to investigate whether CI and arterial stiffness (AS) occur in cardiovascular disease (CVD) patients with SPD. A crosssectional case-control study included hospitalized patients with CVD. Demographic characteristics, CVD and atherogenic risk factors were recorded. SPD was diagnosed by clinical and radiographic dental examinations. Dental clinical attachment level (CAL) and CAL % were recorded. A Mini-Mental State Examination test (MMSE) assessed cognition, a MMSE score of < 27 was set as the cut-off point of CI; a score ≥ 27 was considered as no CI. Patients were categorized into: MMSE<27 (cases) and MMSE≥27 (controls). AS was evaluated by pulse wave velocity (PWV). Serum VCAM-1 levels were determined in a random sample. Results: This study comprised 91 patients (cases, n=26; 29.6%; controls, n=65, 71.4%); aged 73±8 vs. 73±7 years, respectively (p=0.73), of whom 53.8% and 36.9% respectively, were women; SPD was found to be a risk factor for CI; the presence of SPD increased the risk for MMSE <27 by an average 5.39 times (model 1). PWV was associated with MMSE < 27 in the three models. The risk of having MMSE < 27 increased an average of 2.404-fold for each 1-unit increase in PWV. SPD and AS had significant and independent associations on the risk for development CI. MMSE negatively correlated with CAL% (r=0.69) and PWV (r=0.70). PWV positively correlated with CAL% (r=0.67). Serum VCAM-1 levels were higher in SPD with lower MMSE scores. In conclusion, SPD increases the risk of development of cognitive decline in CVD patients. PWV was directly associated with the risk of cognitive decline. These findings denote a significant opportunity to improve periodontal health in order to avert CI in CVD patients.</p>","PeriodicalId":7033,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25317767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A comparative analysis between atraumatic and conventional anesthetic techniques in surgical removal of upper third molars. 非外伤性与常规麻醉方法在上三磨牙手术切除中的比较分析。
Luís Pl Donato, Marcela M Maciel, Luiza M Maciel, Lívia M Lopes, Alessandra de At Carvalho, Hugo Fl de Oliveira, Hélder L Rebelo
{"title":"A comparative analysis between atraumatic and conventional anesthetic techniques in surgical removal of upper third molars.","authors":"Luís Pl Donato,&nbsp;Marcela M Maciel,&nbsp;Luiza M Maciel,&nbsp;Lívia M Lopes,&nbsp;Alessandra de At Carvalho,&nbsp;Hugo Fl de Oliveira,&nbsp;Hélder L Rebelo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pain control is essential in dental practice, and can be accomplished through various techniques. This study seeks to compare atraumatic and conventional anesthetic techniques, applied during surgeries to remove upper third molars. The endpoints evaluated were pain indices, patient satisfaction and anesthetic efficacy. A random parallel split-mouth clinical trial was conducted with 14 patients. Group A received atraumatic anesthesia without a needle (Comfort-in®) and group B received conventional anesthesia by blocking the posterior superior alveolar nerve (PSAN) and Greater Palatine Nerve (GPN). A Visual Analogue Scale (VAS) was used to assess pain. A significantly (p<0.001) lower perception of pain was observed among individuals who received the atraumatic technique. In 71% of cases, it was necessary to supplement the anesthesia during the procedure. Even considering the need for additional anesthesia, the Comfort-in® technique was more accepted by patients with regard to pain perception than the conventional manual technique.</p>","PeriodicalId":7033,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25317769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Brazilian dentists' perceptions of using bone grafts: an inland survey. 巴西牙医对骨移植的看法:一项内陆调查。
Acta odontologica latinoamericana : AOL Pub Date : 2020-12-01 DOI: 10.54589/aol.33/3/165
L. K. Araújo, Gustavo S Antunes, M. M. Melo, Igor Iuco Castro‐Silva
{"title":"Brazilian dentists' perceptions of using bone grafts: an inland survey.","authors":"L. K. Araújo, Gustavo S Antunes, M. M. Melo, Igor Iuco Castro‐Silva","doi":"10.54589/aol.33/3/165","DOIUrl":"https://doi.org/10.54589/aol.33/3/165","url":null,"abstract":"Pre-clinical assessments of bone substitute materials are frequent in the literature, but research papers about the clinical situation of bone graft use and consumer market acceptance are rare. The aim of this study was to evaluate the dental use of bone grafts according to the perception of dentists in the city of Sobral, Brazil. We interviewed 183 professionals and analyzed their professional data, knowledge of the subject, specific use, and opinions on cost-effectiveness and biosafety. Most of the respondents had 10 years' or less experience in the profession, and lacked specialization though they had been familiar with the subject since they graduated. The most frequently mentioned compositions were ceramics, followed by composites. Only a quarter of the respondents had performed bone grafts, generally with up to 50 cases, with the most frequent applications being using simultaneously with a dental implant, fresh dental sockets and maxillary sinus lifting. Autogenous and xenogeneic grafts were more frequent than alloplastic and allogeneic; ceramics were the most frequently used composition, and the association of bone graft with membrane was more frequent than bone graft alone or associated with autologous fibrin. Professional and patient satisfaction was high, cost was considered moderate, and differences were found regarding patient participation in the choice and country of origin of the product. Bone grafts were used more often in the private than public service. Dentists stated that they follow the instructions. Inflammatory or infectious complications were found to be related to the frequency of the procedure performed and safety regarding the origin. Opinions were favorable regarding the use of grafts derived from animals and unfavorable to grafts derived from cadavers. Very few respondents were registered in human bone tissue banks. The good level of acceptance for dental use of bone grafts in a Brazilian inland city provides a promising scenario for the development of the sector.","PeriodicalId":7033,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82229549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Signs of alveolar bone damage in early stages of periodontitis and its prevention by stimulation of cannabinoid receptor 2. Model in rats. 牙周炎早期牙槽骨损伤的迹象及其通过刺激大麻素受体2的预防。大鼠模型。
César A Ossola, Julieta A Rodas, Noelia B Balcarcel, Julia I Astrauskas, Juan C Elverdin, Javier Fernández-Solari
{"title":"Signs of alveolar bone damage in early stages of periodontitis and its prevention by stimulation of cannabinoid receptor 2. Model in rats.","authors":"César A Ossola,&nbsp;Julieta A Rodas,&nbsp;Noelia B Balcarcel,&nbsp;Julia I Astrauskas,&nbsp;Juan C Elverdin,&nbsp;Javier Fernández-Solari","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aims of the present study were, first, to identify signs of alveolar bone damage in early stages of experimental periodontitis (EP) and, second, to assess its possible prevention by treatment with cannabinoid receptor 2 agonist HU 308. Experimental periodontitis was induced by injections of lipopolysaccharide (LPS) (1mg/ml) in gums surrounding maxillary and mandibular first molar, 3 days per week, and untreated controls were kept for comparison. Then, a 3-week study was conducted including eighteen new rats (six rats per group): 1) controls; 2) experimental periodontitis rats; and 3) experimental periodontitis rats treated daily with HU 308 (500 ng/ml). After euthanasia, alveolar bone loss was assessed by morphometric and histomorphometric techniques, and the content of prostaglandin E2 (PGE2) in gingival tissue was evaluated by radioimmunoassay. The first signs of alveolar bone loss were apparent at 3 weeks of experimental periodontitis (ρ<0.05) in the mandibular first molar, but there was no detectable change at 1 week, leading us to establish 3 weeks as an early stage of experimental periodontitis. Rats subjected to 3-week experimental periodontitis showed less interradicular bone volume, less whole bone perimeter and fewer bone formation areas, and higher periodontal space height, bone resorption areas, number of osteoclasts and gingival content of prostaglandin E2 than controls, while HU 308 prevented, at least partially, the deleterious effects (ρ<0.001). We can conclude that a 3-week term of lipopolysaccharide-induced periodontitis in rats provides a valid model of the early stage of the disease, as emerging damage is observed in bone tissue. Furthermore, harmful effects at 3 weeks could be prevented by local stimulation of cannabinoid receptor 2, before greater damage is produced.</p>","PeriodicalId":7033,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38372266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Post preparation: cleanness achieved by different irrigating protocols. 后处理:通过不同的冲洗方案达到清洁。
Acta odontologica latinoamericana : AOL Pub Date : 2020-09-01 DOI: 10.54589/aol.33/2/117
Marcela L Roitman, M. Picca, R. Macchi
{"title":"Post preparation: cleanness achieved by different irrigating protocols.","authors":"Marcela L Roitman, M. Picca, R. Macchi","doi":"10.54589/aol.33/2/117","DOIUrl":"https://doi.org/10.54589/aol.33/2/117","url":null,"abstract":"The aim of this study was to evaluate the ability of different irrigation protocols to remove from the walls of the root canal and entrance to dentinal tubules the smear layer produced during preparation for a fiber post. Fifty decoronated human lower premolars were treated endodontically and the apical third of the canal was filled with a single gutta-percha point using warm vertical compaction. Ten millimeters were left free for post preparation, which was done by sequential use of a Largo #1 drill and Exacto #3 pilot drill (Angelus, Brazil), with irrigation with distilled water upon each instrument change. Samples were distributed randomly into 5 groups (n=10). G 1: No irrigation after post preparation. G 2: Distilled water activated for 60 s + distilled water. G 3: 5.25% NaOCl 15 s+ distilled water. G 4: 17% EDTA (Farmadental, Argentina) 60 s + 5.25% NaOCl 15 s + distilled water. G 5: 10% polyacrylic acid (Densell) 15 s + distilled water. Irrigant was activated mechanically with a low-speed conical brush. Roots were then split longitudinally in vestibular-lingual direction. Each surface was observed under SEM in its different thirds. Microphotographs were taken at 150X and 600X magnification and results analyzed statistically using Kruskall Wallis and Friedman tests (p<0.05). The results [mean (standard deviation)] were: for 150X: G1 11.00 (1.33), G2 7.50 (3.13), G3 6.30 (2.58), G4 2.20 (2.80), G5 4.30 (1.50), and for 600X G1 11.40 (0.84), G2 10.00 (1.94), G3 7.70 (3.33), G4 5.80 (3.70), G5 7.20 (2.65). The statistical analysis showed significant differences between irrigants (p<0.05) but not between root thirds (p>0.05). The EDTA+NaOCl combination and polyacrylic acid showed greater capacity to remove the smear layer created during post preparation.","PeriodicalId":7033,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78845459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Enzyme production by Candida albicans and Candida dubliniensis in periodontal HIV-positive patients receiving and not receiving antiretroviral therapy. 白色念珠菌和都柏林念珠菌在接受和未接受抗逆转录病毒治疗的牙周hiv阳性患者中的酶生产。
Acta odontologica latinoamericana : AOL Pub Date : 2020-09-01 DOI: 10.54589/aol.33/2/104
Verónica A Dubois, M. Gonzalez, M. E. Martínez, Laura Fedelli, Sabrina Lamas, Luciana R D Eramo, A. Squassi, G. Sánchez, P. Salgado, L. Gliosca, S. Molgatini
{"title":"Enzyme production by Candida albicans and Candida dubliniensis in periodontal HIV-positive patients receiving and not receiving antiretroviral therapy.","authors":"Verónica A Dubois, M. Gonzalez, M. E. Martínez, Laura Fedelli, Sabrina Lamas, Luciana R D Eramo, A. Squassi, G. Sánchez, P. Salgado, L. Gliosca, S. Molgatini","doi":"10.54589/aol.33/2/104","DOIUrl":"https://doi.org/10.54589/aol.33/2/104","url":null,"abstract":"Candida dubliniensis (Cd) and Candida albicans (Ca) are the most frequently isolated yeasts in HIV+ patients. Some of the enzymes produced by these yeasts are considered virulence factors since they contribute to pathogenicity of Candida spp. The aim of the present study was to compare production of enzymes such as phospholipase (Ph), proteinase (P), and hemolysin (H) by Cd and Ca strains isolated from periodontal HIV-positive patients receiving and not receiving highly active antiretroviral therapy (HAART). Subgingival biofilm samples were obtained using paper points, and a sample of oral mucosa was taken using a swab. Phenotypic and molecular methods were used to isolate 39 strains of Candida, including 25 strains of Cd and 14 strains of Ca, obtained from 33 periodontal pocket samples and 6 oral mucosa samples collected from 15 HIV+ patients (8 receiving and 7 not receiving HAART). Malt egg-yolk agar, albumin agar and blood agar were used to evaluate pH, P and H production respectively. The strains were inoculated in duplicate and incubated at 37 ºC. Colony and halo diameters were measured. A greater proportion of Ca was observed in patients not receiving HAART, and a higher proportion of Cd was observed in those under HAART, Chi2 p< 0.001. Phospholipase production was observed in 92.9% percent of isolated Ca strains but in none of the isolated Cd strains. Proteinase production was high in Ca and Cd strains isolated from patients not receiving HAART. Hemolysin production was observed in all the studied strains, though it was significantly higher (p=0.04) in Ca and Cd strains isolated from patients not receiving HAART. To sum up, the proportion of Candida dubliniensis strains was highest in the subgingival biofilm of patients receiving HAART, and Cd strains were found to express fewer virulence factors than Ca strains.","PeriodicalId":7033,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85338650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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