Acta Europaea fertilitatis最新文献_第7页

GnRH analogs versus expectant management in minimal and mild endometriosis-associated infertility. GnRH类似物与轻度和轻度子宫内膜异位症相关不孕症的预期治疗。

Acta Europaea fertilitatis Pub Date : 1994-01-01

R Marana, F V Paielli, L Muzii, S Dell'Acqua, S Mancuso

引用次数: 0

Operative ultrasonography. 有效的超声。

Acta Europaea fertilitatis Pub Date : 1994-01-01

G B La Sala

引用次数: 0

Clinical evaluation of male factor: what is critical? 男性因素的临床评价:什么是关键?

Acta Europaea fertilitatis Pub Date : 1993-11-01

R Schoysman

引用次数: 0

Sperm physiology: new developments. 精子生理学:新进展。

Acta Europaea fertilitatis Pub Date : 1993-11-01

D S Irvine

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Gametes micromanipulation. 配子精密控制。

Acta Europaea fertilitatis Pub Date : 1993-11-01

J Cohen

引用次数: 0

Pharmacologic treatment of idiopathic oligoasthenoteratozoospermia. 特发性少弱无畸形精子症的药物治疗。

Acta Europaea fertilitatis Pub Date : 1993-11-01

S Francavilla, A Gabriele, M Oliveri, G Cordeschi, G Properzi

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Enhancing sperm fertilizing capacity in vitro: indications and limits. 提高精子体外受精能力:适应症和局限性。

Acta Europaea fertilitatis Pub Date : 1993-11-01

L L Veeck

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Epididymal aspiration of nemasperms (MESA). 附睾抽吸(MESA)。

Acta Europaea fertilitatis Pub Date : 1993-11-01

R Schoysman, E Cittadini, R Palermo, G B La Sala

引用次数: 0

Mechanisms involved in the decidualization of human endometrial stromal cells. 人子宫内膜间质细胞脱个体化的机制。

Acta Europaea fertilitatis Pub Date : 1993-09-01

B Tang, S Guller, E Gurpide

{"title":"Mechanisms involved in the decidualization of human endometrial stromal cells.","authors":"B Tang, S Guller, E Gurpide","doi":"","DOIUrl":"","url":null,"abstract":"Nearly homogeneous preparations of stromal cells derived from human proliferative endometrium can be obtained by treating endometrial fragments with collagenase in order to disperse stromal elements, filtering the mixture a 25 microns opening sieve to separate them from gland, and incubating the dispersed cells to culture dishes. Exposure of stromal cell cultures to db-cAMP, 8-Br-cAMP or forskolin in RPMI 1640 medium containing 2% ct-FCS and 0.1 U/ml insulin induces the expression of prolactin (PRL), evident from 1) its secretion to the culture medium, measured by radioimmunoassay and by Western blot analysis, 2) the incorporation of 35S-methionine in a protein precipitated with a PRL antibody and co-migrating with authentic PRL during electrophoresis, and 3) the synthesis of PRL mRNA determined by Northern blot analysis. The cAMP effect on PRL production is enhanced by progestins, which by themselves are weak PRL inducers under similar experimental conditions. As expected from previous findings in our laboratory, showing that addition of PRL to the culture medium induces decidualization of endometrial stromal cells, cAMP derivatives not only induce PRL but also provoke differentiation of the fibroblast-like stromal cells to the decidual phenotype, as evident from morphologic changes and by the expression of products characteristic of decidual cells, e.g. IGFBP-1, desmin, hsp 27 and laminin. These findings suggest a PRL-mediated, progesterone-enhanced decidualization mechanism initiated by physiologic agents increasing cAMP levels in stromal cells.","PeriodicalId":7016,"journal":{"name":"Acta Europaea fertilitatis","volume":"24 5","pages":"221-3"},"PeriodicalIF":0.0,"publicationDate":"1993-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18980418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors affecting human blastocyst formation in vitro and freezing at the blastocyst stage. 影响人胚泡体外形成及胚泡期冷冻的因素。

Acta Europaea fertilitatis Pub Date : 1993-09-01

Y J Menezo, B Nicollet, M Dumont, A Hazout, L Janny

{"title":"Factors affecting human blastocyst formation in vitro and freezing at the blastocyst stage.","authors":"Y J Menezo, B Nicollet, M Dumont, A Hazout, L Janny","doi":"","DOIUrl":"","url":null,"abstract":"Whatever the culture medium, embryo culture generally leads to a major loss of viability in mouse, rabbits even if the morphological development of the embryo is preserved. Moreover, Embryo metabolism is commonly depressed in culture media. The protein turnover is accelerated and the quality of the metabolites transport systems is impaired. Various coculture systems have been designed to avoid this loss of viability and in some animal species, to overcome the so called \"embryo developmental arrest\" usually observed at the approximate time of genomic activation. Moreover, it is clear now that cocultured embryos have usually higher cells numbers than those observed for embryos cultured in classical culture media. In the human, the problems seem less complicated because embryos can be transferred into the uterus on the second day post fertilization, at a time when they would normally be in the Fallopian tube: this is not possible in animal species. Also, blastocysts can be obtained, even at low rates, in conventional culture media and there is no apparent block of development. In this paper, we will present an overview of Cocultures in different species. Then, we will focus on the Human including the blastocyst formation rate and freezing at the Blastocyst stage. At the beginning of the Story, For coculturing, 2 ideas were put forward: The use of embryonic tissue (trophoblast) to help the embryo through an autocrine effect. The use of female genital tract cells, to assist the embryo through a paracrine effect.","PeriodicalId":7016,"journal":{"name":"Acta Europaea fertilitatis","volume":"24 5","pages":"207-13"},"PeriodicalIF":0.0,"publicationDate":"1993-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18980412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0