Acta cirurgica brasileira最新文献_第3页

A realistic model for vasectomy reversal training using swine testicles. 猪睾丸输精管结扎逆转训练的现实模型。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2023-01-01 DOI: 10.1590/acb383023

Deivid Ramos Dos Santos, Wender de Jesus Pena Corrêa Junior, Lívia Guerreiro de Barros Bentes, Rafael Silva Lemos, Victor Matheus Mendonça de Araújo, Gabrielly Leite Andrade, Renan Kleber Costa Teixeira, Luís Otávio Amaral Duarte Pinto, Herick Pampolha Huet de Bacelar

{"title":"A realistic model for vasectomy reversal training using swine testicles.","authors":"Deivid Ramos Dos Santos, Wender de Jesus Pena Corrêa Junior, Lívia Guerreiro de Barros Bentes, Rafael Silva Lemos, Victor Matheus Mendonça de Araújo, Gabrielly Leite Andrade, Renan Kleber Costa Teixeira, Luís Otávio Amaral Duarte Pinto, Herick Pampolha Huet de Bacelar","doi":"10.1590/acb383023","DOIUrl":"https://doi.org/10.1590/acb383023","url":null,"abstract":"Purpose: To evaluate the viability of the porcine vas deferens as a realistic microsurgical training model for vasectomy reversal.Methods: The model uses swine testicles (vas deferent), which are usually discarded in large street markets since they are not part of Brazilian cuisine. The spermatic cord was carefully dissected, and the vas deferens were isolated, measuring 10 cm in length. A paper quadrilateral with 5 cm2 was built to delimit the surgical training field. The objective of the model is to simulate only the microsurgical step when the vas deferens are already isolated. The parameters analyzed were: feasibility for reproducing the technique, patency before and after performing the vasovasostomy, cost of the model, ease of acquisition, ease of handling, execution time, and model reproducibility.Results: The simulator presented low cost. All models made were viable with a texture similar to human, with positive patency obtained in 100% of the procedures. The internal and external diameters of the vas deferens varied between 0.2-0.4 mm and 2-3 mm, respectively, with a mean length of 9 ± 1.2 cm. The total procedure time was 43.28 ± 3.22 minutes.Conclusions: The realistic model presented proved to be viable for carrying out vasectomy reversal training, due to its low cost, easy acquisition, and easy handling, and providing similar tissue characteristics to humans.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e383023"},"PeriodicalIF":1.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10136977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The effect of olsalazine of chinese generic drugs on ulcerative colitis induced by dextran sulfate sodium salt in BALB/c mice. 中药仿制药奥萨拉嗪对硫酸葡聚糖钠盐致BALB/c小鼠溃疡性结肠炎的影响。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2023-01-01 DOI: 10.1590/acb382923

Zheng-Yong Yu, Yu-Sheng Xu, Miao Tang, Wen-Feng Xin

{"title":"The effect of olsalazine of chinese generic drugs on ulcerative colitis induced by dextran sulfate sodium salt in BALB/c mice.","authors":"Zheng-Yong Yu, Yu-Sheng Xu, Miao Tang, Wen-Feng Xin","doi":"10.1590/acb382923","DOIUrl":"https://doi.org/10.1590/acb382923","url":null,"abstract":"Purpose: To explore effect and mechanism of olsalazine of Chinese generic drugs on ulcerative colitis induced by dextran sulfate sodium salt (DSS) in BALB/c mice.Methods: The mouse model of ulcerative colitis was induced by free drinking of 3% (w/v) DSS aqueous solution for seven days. The mice were treated with olsalazine (0.6 g·kg-1) of Chinese generic drugs. The therapeutic effect of olsalazine on ulcerative colitis mice was evaluated by measuring disease activity index (DAI), colonic mucosal injury index (CMDI), histopathological score (HS), and detected the expression levels of interleukin (IL)-2, IL-10, tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), IL-1β in serum and IL-7, IL-17, IL-22, epidermal growth factor (EGF), transforming growth factor β1 (TGF-β1) in colonic homogenate of mice.Results: Olsalazine significantly increased the contents of IL-2, IL-10, IL-22, TGF and EGF in ulcerative colitis rats, and significantly decreased the scores of DAI, CMDI, HS and the contents in IL-7, IL-17, TNF-α, IL-1β and IFN-γ when compared with the model group. It improved the degree of colonic lesion in ulcerative colitis mice.Conclusions: It was suggested that olsalazine has a therapeutic effect on ulcerative colitis induced by DSS in mice, and the mechanism may be related to the increase of IL-2, IL-10, IL-22, TGF, and EGF and the decrease of the expression of IL-7, IL-17, TNF-α, IL-1β, and IFN-γ.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e382923"},"PeriodicalIF":1.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443231/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10187601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of local and remote ischemic postconditioning methods on ischemiareperfusion injury in a young animal model of acute mesenteric ischemia. 局部和远程缺血后适应方法对急性肠系膜缺血幼龄动物模型缺血再灌注损伤的影响。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2023-01-01 DOI: 10.1590/acb381323

Mateus Souza Abreu, Ana Cristina Aoun Tannuri, Rafael Felipe Gonçalves Rodrigues, Rafael José da Silva, Josiane de Oliveira Gonçalves, Suellen Serafini, Uenis Tannuri

{"title":"Effects of local and remote ischemic postconditioning methods on ischemiareperfusion injury in a young animal model of acute mesenteric ischemia.","authors":"Mateus Souza Abreu, Ana Cristina Aoun Tannuri, Rafael Felipe Gonçalves Rodrigues, Rafael José da Silva, Josiane de Oliveira Gonçalves, Suellen Serafini, Uenis Tannuri","doi":"10.1590/acb381323","DOIUrl":"https://doi.org/10.1590/acb381323","url":null,"abstract":"Purpose: Acute mesenteric ischemia (AMI) is a condition in pediatric surgery that ranges from intestine necrosis to death. Ischemic postconditioning (IPoC) methods were developed to reduce the damage caused by revascularization. This study aimed to evaluate the efficacy of these methods in an experimental weaning rat model.Methods: Thirty-two 21-day-old Wistar rats were allocated into four groups according to the surgical procedure performed: control, ischemia-reperfusion injury (IRI), local (LIPoC) and remote IPoC (RIPoC). At euthanasia, fragments of the intestine, liver, lungs, and kidneys were submitted to histological, histomorphometric, and molecular analyses.Results: In the duodenum, intestines, and kidneys histological alterations promoted by IRI were reversed by remote postconditioning method. In the distal ileum, the histomorphometric alterations could be reversed by the postconditioning methods with more evident effects promoted by the remote method. The molecular analysis found that the levels of expression of Bax (proapoptotic) and Bcl-XL (antiapoptotic) genes in the intestine were increased by IRI. These alterations were equally reversed by the postconditioning methods, with more evident effects of the remote method.Conclusions: IPoC methods positively reduced the damage caused by IRI in weaning rats.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e381323"},"PeriodicalIF":1.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10238078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9954007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Er-Bai-Tang decoction improved the movement disorders and neuronal injury in the Parkinson's disease model rats via decreasing p38 MAPK pathway and improving the composition of intestinal flora. 二百汤通过降低p38 MAPK通路和改善肠道菌群组成改善帕金森病模型大鼠的运动障碍和神经元损伤。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2023-01-01 DOI: 10.1590/acb371104

Jinrong Li, Yuehan Ni, Li Huang, Xinyuan Yu, Jianwei Zhu

{"title":"Er-Bai-Tang decoction improved the movement disorders and neuronal injury in the Parkinson's disease model rats via decreasing p38 MAPK pathway and improving the composition of intestinal flora.","authors":"Jinrong Li, Yuehan Ni, Li Huang, Xinyuan Yu, Jianwei Zhu","doi":"10.1590/acb371104","DOIUrl":"https://doi.org/10.1590/acb371104","url":null,"abstract":"Purpose: Our previous study showed that Er-Bai-Tang decoction (EBT) could effectively improve Parkinson's disease (PD) patients' quality of life, sleep, mood, and cognitive disorders, but the mechanism of EBT to treat PD was unclear. So, our study aimed to explore the mechanism of EBT to treat PD via p38 mitogen-activated protein kinases (MAPK) pathway and intestinal flora.Methods: In our study, the PD rat model was established by subcutaneously injecting 2 mg/kg/d rotenone solution, and 23.43 g/kgEBT was used to treat PD model rats.Results: Behavioral test showed that EBT could reverse the motor impairment in the PD model rats. Hematoxylin and eosin result showed that EBT could reduce the cell necrosis in the SNpc area of the PD model rats. Western blotting and real time-polymerase chain reaction showed that EBT could decrease the p38 MAPK expression in the SNpc area of the PD model rats. 16s rRNA sequencing analysis showed that EBT could improve the composition of intestinal flora in the PD model rats. Rikenellaceae at family level and Alistipes and Allobaculum at the genus level were the key species in the PD development and EBT treatment to PD. KEGG showed that EBT might change the iron uptake in PD rats.Conclusions: EBT could improve the motor symptoms and neuronal injury in the PD model rat, and its mechanism may be related to decreasing p38 MAPK pathway and improving the composition of intestinal flora.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"37 11","pages":"e371104"},"PeriodicalIF":1.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9829241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9083685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyanidin-3-O-glucoside plays a protective role against renal ischemia/ reperfusion injury via the JAK/STAT pathway. 花青素-3- o -葡萄糖苷通过JAK/STAT通路对肾缺血再灌注损伤起保护作用。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2023-01-01 DOI: 10.1590/acb381023

Yufeng Xiong, Jun Jian, Honglin Yu, Jiejun Wu, Hu Mao, Ruikang Feng, Lei Wang, Yonghong Jian, Xiuheng Liu

{"title":"Cyanidin-3-O-glucoside plays a protective role against renal ischemia/ reperfusion injury via the JAK/STAT pathway.","authors":"Yufeng Xiong, Jun Jian, Honglin Yu, Jiejun Wu, Hu Mao, Ruikang Feng, Lei Wang, Yonghong Jian, Xiuheng Liu","doi":"10.1590/acb381023","DOIUrl":"https://doi.org/10.1590/acb381023","url":null,"abstract":"Purpose: To investigate the role of cyanidin-3-O-glucoside (C3G) in renal ischemia/reperfusion (I/R) injury and the potential mechanisms.Methods: Mouse models were established by clamping the left renal vessels, and in vitro cellular models were established by hypoxic reoxygenation.Results: Renal dysfunction and tissue structural damage were significantly higher in the I/R group. After treatment with different concentrations of C3G, the levels of renal dysfunction and tissue structural damage decreased at different levels. And its protective effect was most pronounced at 200 mg/kg. The use of C3G reduced apoptosis as well as the expression of endoplasmic reticulum stress (ERS)-related proteins. Hypoxia/reoxygenation (H/R)-induced apoptosis and ERS are dependent on oxidative stress in vitro. In addition, both AG490 and C3G inhibited the activation of JAK/STAT pathway and attenuated oxidative stress, ischemia-induced apoptosis and ERS.Conclusions: The results demonstrated that C3G blocked renal apoptosis and ERS protein expression by preventing reactive oxygen species (ROS) production after I/R via the JAK/STAT pathway, suggesting that C3G may be a potential therapeutic agent for renal I/R injury.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e381023"},"PeriodicalIF":1.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10158851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9405841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Puerarin alleviated oxidative stress and ferroptosis during renal fibrosis induced by ischemia/reperfusion injury via TLR4/Nox4 pathway in rats. 葛根素通过TLR4/Nox4通路减轻大鼠缺血再灌注损伤肾纤维化过程中的氧化应激和铁凋亡。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2023-01-01 DOI: 10.1590/acb382523

Jun Jian, Dan Wang, Yufeng Xiong, Jingsong Wang, Qingyuan Zheng, Zhengyu Jiang, Jiacheng Zhong, Song Yang, Lei Wang

{"title":"Puerarin alleviated oxidative stress and ferroptosis during renal fibrosis induced by ischemia/reperfusion injury via TLR4/Nox4 pathway in rats.","authors":"Jun Jian, Dan Wang, Yufeng Xiong, Jingsong Wang, Qingyuan Zheng, Zhengyu Jiang, Jiacheng Zhong, Song Yang, Lei Wang","doi":"10.1590/acb382523","DOIUrl":"https://doi.org/10.1590/acb382523","url":null,"abstract":"Purpose: To investigate the role of puerarin on renal fibrosis and the underlying mechanism in renal ischemia and reperfusion (I/R) model.Methods: Rats were intraperitoneally injected with puerarin (50 or 100 mg/kg) per day for one week before renal I/R. The level of renal collagen deposition and interstitial fibrosis were observed by hematoxylin and eosin and Sirius Red staining, and the expression of α-smooth muscle actin (α-SMA) was examined by immunohistochemical staining. The ferroptosis related factors and TLR4/Nox4-pathway-associated proteins were detected by Western blotting.Results: Puerarin was observed to alleviate renal collagen deposition, interstitial fibrosis and the α-SMA expression induced by I/R. Superoxide dismutase (SOD) activities and glutathione (GSH) level were decreased in I/R and hypoxia/reoxygenation (H/R), whereas malondialdehyde (MDA) and Fe2+ level increased. However, puerarin reversed SOD, MDA, GSH and Fe2+ level changes induced by I/R and H/R. Besides, Western blot indicated that puerarin inhibited the expression of ferroptosis related factors in a dose-dependent manner, which further demonstrated that puerarin had the effect to attenuate ferroptosis. Moreover, the increased expression of TLR/Nox4-pathway-associated proteins were observed in I/R and H/R group, but puerarin alleviated the elevated TLR/Nox4 expression.Conclusions: Our results suggested that puerarin inhibited oxidative stress and ferroptosis induced by I/R and, thus, delayed the progression of renal fibrosis, providing a new target for the treatment of renal fibrosis.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e382523"},"PeriodicalIF":1.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10403246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10331125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Does low-intensity pulsed ultrasound accelerate phasic calcium phosphate ceramic-induced bone formation? 低强度脉冲超声是否加速相性磷酸钙陶瓷诱导的骨形成?

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2023-01-01 DOI: 10.1590/acbe380023

Lanying Sun, Xiaoshuang Guo, Qibao Wang, Zhongshuai Shang, Yi Du, Guodong Song

{"title":"Does low-intensity pulsed ultrasound accelerate phasic calcium phosphate ceramic-induced bone formation?","authors":"Lanying Sun, Xiaoshuang Guo, Qibao Wang, Zhongshuai Shang, Yi Du, Guodong Song","doi":"10.1590/acbe380023","DOIUrl":"https://doi.org/10.1590/acbe380023","url":null,"abstract":"Purpose: Low-intensity pulsed ultrasound (LIPUS) has been used to stimulate the healing of the fresh fracture, delayed union, and non-union in both animal and clinical studies. Besides, biphasic calcium phosphate ceramic (BCP) is a promising biomaterial for bone repair as it shows favorable biocompatibility, osteoinduction, and osteoconduction. However, scarcity is known about the combined effect of LIPUS and BCP on bone formation.Methods: The combined effect of LIPUS and BCP was studied in a beagle model. Twelve dogs were used. BCP granules without any additions were implanted into bilateral erector spinae muscles. One side is the BCP group, while the counterlateral side is LIPUS + BCP group. Histological and histomorphometric analyses, and quantitative real-time polymerase chain reaction were evaluated.Results: Compared with BCP alone, the LIPUS + BCP showed no advantages in early bone formation. Furthermore, the Notch signaling pathway-related mRNA has no significant difference between the two groups.Conclusions: The preliminary results showed that the BCP, which has intrinsic osteoinduction nature, was an effective and promising material. However, LIPUS has no enhanced effect in BCP induced ectopic bone formation. Furthermore, LIPUS has no effect on the Notch signaling pathway. Whether costly LIPUS could be used in combination with BCP should be a rethink.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e380023"},"PeriodicalIF":1.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9237755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical and immunohistochemical examination of the effects of ephedrine in rat ovary tissue. 麻黄碱对大鼠卵巢组织作用的生化及免疫组化检测。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2023-01-01 DOI: 10.1590/acb381523

Veysel Toprak, Senem Alkan Akalın, Ece Öcal, Yunus Çavuş, Engin Deveci

{"title":"Biochemical and immunohistochemical examination of the effects of ephedrine in rat ovary tissue.","authors":"Veysel Toprak, Senem Alkan Akalın, Ece Öcal, Yunus Çavuş, Engin Deveci","doi":"10.1590/acb381523","DOIUrl":"https://doi.org/10.1590/acb381523","url":null,"abstract":"Purpose: It was aimed to investigate the biochemical and immunohistochemical effects of ephedrine (EPH) in bilateral ovariectomized rats.Methods: Twenty-four Sprague Dawley female rats were divided into three groups: control group: The abdomen was opened and closed without any treatment; ischemia-reperfusion (IR) group: 2 h of ischemia followed by 2 h of reperfusion were allowed to cause IR injury; IR+EPH group: oral EPH solution (5 mg/kg) was administered for 28 days.Results: Biochemical parameters were statistically significant in group comparisons. Increased interleukin-6 (IL-6) expression, degenerative preantral and antral follicle cells and inflammatory cells around blood vessels were seen in IR group. Negative IL-6 expression was observed in seminal epithelial cells, preantral and antral follicle cells in IR+EPH group. While caspase-3 activity increased in granulosa cells and stromal cells in IR group, caspase-3 expression was negative in preantral and antral follicle cells in the germinal epithelium and cortex in IR+EPH group.Conclusions: The effect of apoptosis, which occurs with the signaling that starts in the cell nucleus, caused the cessation of the stimulating effect at the nuclear level after EPH administration, and a decrease in the antioxidative effect in IR damage and inflammation in the apoptotic process.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e381523"},"PeriodicalIF":1.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10158848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9413424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathological, ultrastructural, and immunohistochemical examination of changes in the placenta as a result of severe preeclampsia. 重度子痫前期胎盘组织病理学、超微结构及免疫组化检查。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2023-01-01 DOI: 10.1590/acb382023

Çağdaş Özgökçe, Aydın Öcal, Işılay Seze Ermiş, Engin Deveci

{"title":"Histopathological, ultrastructural, and immunohistochemical examination of changes in the placenta as a result of severe preeclampsia.","authors":"Çağdaş Özgökçe, Aydın Öcal, Işılay Seze Ermiş, Engin Deveci","doi":"10.1590/acb382023","DOIUrl":"https://doi.org/10.1590/acb382023","url":null,"abstract":"Purpose: To investigate the role of hypoxia-inducible transcription factor-1 alpha (HIF-1α) and angiogenetic factor endothelin-1 (ET-1) expression in regulating hypoxia and placental development by routine histopathological methods.Methods: Twenty preeclamptic and normal placentas were used. Placenta tissue pieces were examined histopathologically after routine paraffin follow-ups. HIF-1α and ET-1 proteins were examined immunohistochemically, and placental tissues were examined ultrastructurally.Results: Increase in syncytial proliferation, endothelial damage in vessels, and increase in collagen were observed in preeclamptic placentas. As a result of preeclampsia, an increase was observed in HIF-1α and ET-1 protein levels in the placenta. Dilatation of endoplasmic reticulum and loss of cristae in mitochondria were observed in trophoblast cells in preeclamptic placental sections.Conclusions: High regulation of oxygen resulting from preeclampsia has been shown to be a critical determinant of placentagenesis and plays an important role in placental differentiation, changes in maternal and fetal blood circulation, trophoblastic invasion, and syncytial node increase. It has been thought that preeclampsia affects secretion by disrupting the endoplasmic reticulum structure and induces mitochondrial damage, and that ET-1 may potentially help in the induction of stress pathways as a result of hypoxia in preeclampsia.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e382023"},"PeriodicalIF":1.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10191158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9540919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Columbianadin attenuates doxorubicin-induced cardiac injury, oxidative stress, and apoptosis via Sirt1/FOXO1 signaling pathway. Columbianadin通过Sirt1/FOXO1信号通路减弱阿霉素诱导的心脏损伤、氧化应激和细胞凋亡。

IF 1.1 4区医学

Acta cirurgica brasileira Pub Date : 2023-01-01 DOI: 10.1590/acb382223

Bo Peng, Li Rao, Jiaolong Yang, Xiaowei Ku, Bin Kong, Wei Shuai, He Huang

{"title":"Columbianadin attenuates doxorubicin-induced cardiac injury, oxidative stress, and apoptosis via Sirt1/FOXO1 signaling pathway.","authors":"Bo Peng, Li Rao, Jiaolong Yang, Xiaowei Ku, Bin Kong, Wei Shuai, He Huang","doi":"10.1590/acb382223","DOIUrl":"https://doi.org/10.1590/acb382223","url":null,"abstract":"Purpose: Oxidative stress and apoptosis contribute to the pathological basis of doxorubicin (DOX)-induced cardiotoxicity. Columbianadin (CBN) is one of the main bioactive constituents isolated from the root of Angelica pubescens. Herein, we intended to explore the potential role and molecular basis of CBN in DOX-induced cardiotoxicity.Methods: C57BL/6 mice were subjected to DOX (15 mg/kg/day, i.p.) to generate DOX-induced cardiotoxicity. CBN (10 mg/kg/day, i.p.) was administered for four week following DOX injection.Results: DOX administered markedly dampened cardiac function, increased cardiac injury, excessive reactive oxygen species (ROS) production, and cardiomyocyte loss. These alterations induced by DOX significantly alleviated by CBN treatment. Mechanistically, our results demonstrated that the CBN exerts cardioprotection role against DOX by up-regulating silent information regulator 1 (Sirt1) and decreasing acetylation of forkhead box O1 (FOXO1). Moreover, Sirt1 inhibition with Ex-527 significantly blunt the beneficial effect of CBN on DOX-induced cardiotoxicity, including cardiac dysfunction, ROS, and apoptosis.Conclusion: Collectively, CBN attenuated oxidative stress and cardiomyocyte apoptosis in DOX-induced cardiotoxicity through maintaining Sirt1/FOXO1 signaling pathway. Our results demonstrated that CBN might be used to treat DOX-related cardiotoxicity.","PeriodicalId":6992,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e382223"},"PeriodicalIF":1.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10292807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9726626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1