Does low-intensity pulsed ultrasound accelerate phasic calcium phosphate ceramic-induced bone formation?

IF 1.1 4区医学 Q3 SURGERY

Acta cirurgica brasileira Pub Date : 2023-01-01 DOI:10.1590/acbe380023

Lanying Sun, Xiaoshuang Guo, Qibao Wang, Zhongshuai Shang, Yi Du, Guodong Song

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Abstract

Purpose: Low-intensity pulsed ultrasound (LIPUS) has been used to stimulate the healing of the fresh fracture, delayed union, and non-union in both animal and clinical studies. Besides, biphasic calcium phosphate ceramic (BCP) is a promising biomaterial for bone repair as it shows favorable biocompatibility, osteoinduction, and osteoconduction. However, scarcity is known about the combined effect of LIPUS and BCP on bone formation.

Methods: The combined effect of LIPUS and BCP was studied in a beagle model. Twelve dogs were used. BCP granules without any additions were implanted into bilateral erector spinae muscles. One side is the BCP group, while the counterlateral side is LIPUS + BCP group. Histological and histomorphometric analyses, and quantitative real-time polymerase chain reaction were evaluated.

Results: Compared with BCP alone, the LIPUS + BCP showed no advantages in early bone formation. Furthermore, the Notch signaling pathway-related mRNA has no significant difference between the two groups.

Conclusions: The preliminary results showed that the BCP, which has intrinsic osteoinduction nature, was an effective and promising material. However, LIPUS has no enhanced effect in BCP induced ectopic bone formation. Furthermore, LIPUS has no effect on the Notch signaling pathway. Whether costly LIPUS could be used in combination with BCP should be a rethink.

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低强度脉冲超声是否加速相性磷酸钙陶瓷诱导的骨形成?

目的:在动物和临床研究中，低强度脉冲超声(LIPUS)已被用于刺激新骨折、延迟愈合和不愈合的愈合。双相磷酸钙陶瓷(biphasic calcium phosphate ceramic, BCP)具有良好的生物相容性、骨诱导和骨传导能力，是一种很有前景的骨修复材料。然而，LIPUS和BCP对骨形成的联合作用尚不清楚。方法:采用beagle模型研究LIPUS和BCP的联合作用。使用了12只狗。将无添加物的BCP颗粒植入双侧竖脊肌。一侧为BCP组，对侧为LIPUS + BCP组。组织学和组织形态计量学分析，定量实时聚合酶链反应进行评估。结果:与单独使用BCP相比，LIPUS + BCP在早期骨形成方面没有优势。此外，Notch信号通路相关mRNA在两组间无显著差异。结论:初步结果表明，BCP具有内在的骨诱导性质，是一种有效的、有发展前景的材料。然而，LIPUS对BCP诱导的异位骨形成没有增强作用。此外，LIPUS对Notch信号通路没有影响。是否昂贵的LIPUS可以与BCP联合使用应该重新考虑。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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