Acta endocrinologica. Supplementum最新文献_第9页

Induction of experimental autoimmune thyroiditis by a 5-10 Kd peptidic fragment from porcine thyroglobulin. 猪甲状腺球蛋白5-10 Kd肽片段诱导实验性自身免疫性甲状腺炎。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01 DOI: 10.1530/acta.0.114s104

J Salamero, J A Boudier, J J Rémy, J Charreire

引用次数: 1

Polymorphism of the immunoglobulin heavy chain T cell receptor beta-chain genes in Graves' disease. 免疫球蛋白重链T细胞受体β链基因在Graves病中的多态性

Acta endocrinologica. Supplementum Pub Date : 1987-01-01 DOI: 10.1530/acta.0.114s149

A Demaine, K I Welsh, B S Hawe, N R Farid

引用次数: 3

Molecular cloning of antigens to thyroid autoantibodies using the expression vector lambda gt11. 利用表达载体lambda gt11克隆甲状腺自身抗体抗原。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01 DOI: 10.1530/acta.0.114s139

B Rapoport, H Hirayu, P Seto, R P Magnusson

{"title":"Molecular cloning of antigens to thyroid autoantibodies using the expression vector lambda gt11.","authors":"B Rapoport, H Hirayu, P Seto, R P Magnusson","doi":"10.1530/acta.0.114s139","DOIUrl":"https://doi.org/10.1530/acta.0.114s139","url":null,"abstract":"The molecular cloning of certain antigens to thyroid autoantibodies present in patients with autoimmune thyroid disease would be of great value in further understanding the pathogenesis of these diseases, and in devising new approaches for their treatment. The ideal method for molecular cloning is to first obtain a partial amino acid sequence of a purified protein and to construct an oligonucleotide probe for screening an appropriate cDNA library. Unfortunately in the case if thyroid autoimmunity, important antigens such as the TSH receptor and the microsomal antigen have not been purified. An alternate approach devised by Young & Davis (1983) is to construct a cDNA library in a vector that expresses the encoded proteins. The library can then be screened with an antibody as a probe. We have constructed cDNA libraries in gt11 using mRNA purified from human, pig and rat thyroid cells. Our experiences in constructing and screening these libraries will be described. The advantages of this system are 1) the protein does not have to be purified, 2) previously unknown antigens may be identified. The disadvantages are 1) lack of specificity with antibody selection, 2) because the cDNA is inserted in the beta-galactosidase gene in the vector the antigen is expressed as a fusion protein. This may disturb the tertiary structure of the antigen and alter its antigenicity, 3) cDNA inserts frequently only contain part of the antigen molecule, and may therefore lack important epitopes; polyclonal antibody may therefore be preferable to monoclonal, 4) only 1 in 6 cDNA inserts will be in the correct reading frame for antigen expression, 5) the expressed protein is not glycosylated.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":6931,"journal":{"name":"Acta endocrinologica. Supplementum","volume":"281 ","pages":"139-45"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.114s139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14092598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Autoimmunity and thyroid growth: methods, concepts and misconceptions. 自身免疫与甲状腺生长:方法、概念和误解。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01 DOI: 10.1530/acta.0.114s299

J E Dumont, P Roger, M Ludgate

引用次数: 3

Pathophysiological and clinical implications of thyroid growth-stimulating immunoglobulins: evidence for their intrathyroidal production. 甲状腺促生长免疫球蛋白的病理生理和临床意义:甲状腺内产生的证据。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01 DOI: 10.1530/acta.0.114s334

H Schatz, I Ludwig, F Wiss, P E Goretzki

引用次数: 1

Thyroid peroxidase is the organ-specific 'microsomal' autoantigen involved in thyroid autoimmunity. 甲状腺过氧化物酶是参与甲状腺自身免疫的器官特异性“微粒体”自身抗原。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01 DOI: 10.1530/acta.0.114s049

J Ruf, B Czarnocka, C De Micco, C Dutoit, M Ferrand, P Carayon

{"title":"Thyroid peroxidase is the organ-specific 'microsomal' autoantigen involved in thyroid autoimmunity.","authors":"J Ruf, B Czarnocka, C De Micco, C Dutoit, M Ferrand, P Carayon","doi":"10.1530/acta.0.114s049","DOIUrl":"https://doi.org/10.1530/acta.0.114s049","url":null,"abstract":"Autoantibodies (aAb) in serum of patients with autoimmune thyroid diseases (AITD) are directed to an antigen associated with thyroid microsomes. Although it has been investigated over almost three decades, the nature of this autoantigen remained unknown. Taking advantage of monoclonal antibodies (mAb) produced in our laboratory, we have demonstrated that thyroid peroxidase (TPO) is the 'microsomal' antigen. Sera of patients with AITD strongly inhibited the binding of only one of 19 mAb raised against human thyroid plasma membranes. This mAb did not react with thyroglobulin but achieved significant binding to preparations of human, bovine and porcine TPO, bovine lactoperoxidase and human myeloperoxidase without altering the enzyme activity. The mAb has been used to immunopurify the human TPO from solubilized thyroid microsomes. The procedure allowed high purification (approximately 3500-fold) of the native enzyme with a reasonable yield (approximately 10 mg TPO/kg thyroid tissue). Human TPO exhibited a specific activity of 350-400 guaiacol U/mg, a peak in the Soret region and a ratio of A411 nm to A280 nm of 0.20-0.25. Upon SDS-polyacrylamide gel electrophoresis, the purified enzyme gave two contiguous bands in the 100 kDa region. Performed in non-reducing conditions, electrophoresis of TPO showed one band in the same 100 kDa region. Sera with aAb to the microsomal antigen immunoprecipitated purified TPO to an extent ranging from 80 to 100% of the initial enzyme amount while sera from normal subjects or from patients with undectable level of anti-microsomal aAb elicit a decrease of less than 30% of the total TPO activity.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":6931,"journal":{"name":"Acta endocrinologica. Supplementum","volume":"281 ","pages":"49-56"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.114s049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14600524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Advances in thyroidology: cell- and immunobiological aspects. Proceedings of an International Merck-Symposium. Lübeck, 2nd to 4th October, 1986. 甲状腺学的进展:细胞和免疫生物学方面。国际默克研讨会论文集。1986年10月2日至4日。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01

引用次数: 0

Polarized properties of thyroid cells: a study with cultured porcine cells. 猪细胞培养甲状腺细胞极化特性的研究。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01 DOI: 10.1530/acta.0.114s220

J Mauchamp, O Chabaud, M Chambard, C Gerard, C Penel, B Verrier

引用次数: 5

Long-acting somatostatin analogue (Sandostatin) reduces late night insulinopenic ketogenesis in diabetic teenagers. 长效生长抑素类似物(山多他汀)减少糖尿病青少年深夜胰岛素缺乏的生酮。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01 DOI: 10.1530/acta.0.115s045

R S Aarsen, G J Bruining, W F Grose, R van Strik, S W Lamberts, A G Harris

{"title":"Long-acting somatostatin analogue (Sandostatin) reduces late night insulinopenic ketogenesis in diabetic teenagers.","authors":"R S Aarsen, G J Bruining, W F Grose, R van Strik, S W Lamberts, A G Harris","doi":"10.1530/acta.0.115s045","DOIUrl":"https://doi.org/10.1530/acta.0.115s045","url":null,"abstract":"Ten diabetic teenagers were admitted into our hospital for two nights, separated by one week. In a double-blind cross-over randomized study they received either 50 micrograms of the new long-acting somatostatin analogue Sandostatin sc or placebo. All patients were between 12 and 16 years of age, C-peptide negative with a duration of diabetes of at least four years. They had either conventional therapy or insulin pump therapy. Insulin doses and diets were kept unchanged. Blood samples were taken half hourly from 17.00 h until 09.30 h the next morning from an indwelling venous catheter. Hormonal and metabolic profiles on the two nights were evaluated by means of a distribution free time sequential co-movement analysis and by the paired Wilcoxon's signed rank test. After Sandostatin was given at 22.00 h, GH levels were significantly suppressed during 4 h. During that period blood glucose was slightly but significantly lower than after placebo. The free-insulin profiles from both nights were very comparable. Co-movement analysis showed a significant correlation between glucose and free insulin variations with a 30-min backward shift of the glucose curve. However, after Sandostatin administration this relation was lost in the period between 22.00 and 07.00 h, indicating a different effect of insulin on glucose levels during the nights Sandostatin was given. Early morning glucose rises were associated with free insulin levels below 20 mU/l. This association was not altered during the Sandostatin nights. Glucagon was not suppressed by Sandostatin except at 120 min after injection, and remained unchanged during the rest of the observation period.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":6931,"journal":{"name":"Acta endocrinologica. Supplementum","volume":"286 ","pages":"45-53"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.115s045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14028415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Concept of a multigenic basis for the pathogenesis of spontaneous autoimmune thyroiditis. 自发性自身免疫性甲状腺炎发病机制的多基因基础概念。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01 DOI: 10.1530/acta.0.114s063

G Wick

{"title":"Concept of a multigenic basis for the pathogenesis of spontaneous autoimmune thyroiditis.","authors":"G Wick","doi":"10.1530/acta.0.114s063","DOIUrl":"https://doi.org/10.1530/acta.0.114s063","url":null,"abstract":"The review presents a concept for the pathogenesis of spontaneous, organ-specific autoimmune diseases that take into account an altered immune regulation, modulating hormonal influences and a genetically determined primary susceptibility of the target organ for the autoimmune attack. The concept is exemplified by means of the Obese strain (OS) chicken model which develops a spontaneous hereditary autoimmune thyroiditis. In respect to the the altered function of the immune system both, MHC associated (Ir) and non-MHC associated genes are involved. The MHC, i.e. a certain haplotype, only plays a modulatory role in determining the frequency and severity of spontaneous autoimmune thyroiditis, while the presence of certain non-MHC associated genes is a absolute prerequisite for the emergence of the disease. The latter is also true for the genetically determined target organ susceptibility, while hormonal factors, notably sex-steroids and glucocorticoids, again only have a facultative, modulatory effect. Only if an appropriate genetic constellation concerning the non-MHC encoded aberrant immunological function and genes coding for the susceptibility of the thyroid gland for the autoimmune process is present, severe autoimmune thyroids develops.","PeriodicalId":6931,"journal":{"name":"Acta endocrinologica. Supplementum","volume":"281 ","pages":"63-9"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14431329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10