{"title":"Establishment of an in vitro method for micropropagation of ironwort, (Sideritis raeseri Boiss. & Heldr.)","authors":"V. Sota, Donald Shuka, S. Bekheet, E. Kongjika","doi":"10.14720/aas.2023.119.2.2738","DOIUrl":"https://doi.org/10.14720/aas.2023.119.2.2738","url":null,"abstract":"Ironwort / Mountain Tea (Sideritis raeseri Boiss & Heldr.) is an endangered (EN) plant species in Albania. This study aimed to develop a rapid clonal propagation protocol using in vitro methodologies. The ironwort seeds were pre-treated with three concentrations of GA3 (250, 500, and 1000 mg l-1). During the inoculation stage, two types of culture media, Murashige & Skoog (MS) and Woody Plant Medium (WPM), were tested, and the effects of both GA3 concentration and culture media used were evaluated. For the subculture stage, three cytokinins (6-benzylaminopurine / BAP, kinetin, zeatin) at four concentrations (0.5; 1.0; 1.5; 2.0 mg l-1), were compared for the RGR index, while for the rooting stage, two different auxins (1-naphthaleneacetic acid / NAA and indole-3-butyric acid / IBA) at four concentrations (0.5; 1.0; 1.5; 2.0 mg l-1) were tested. GA3 at 500 mg l-1 and MS medium resulted as more effective. The highest value of the RGR index during the subculture stage was obtained in the MS nutrient medium supplemented with BAP at 1.5 mg l-1. For rhizogenesis response, IBA was more effective for roots and length number. Based on these results, in vitro methodologies can be a promising tool for the mass production of this endangered plant species and with possible applications for enhancing the production of valuable nutraceuticals.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89145516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shaghayegh T. Beheshti, M. J. Nazarideljou, M. Salehi
{"title":"Water use efficiency, morpho-physiological and biochemical reactions of some bedding plants to drought stress","authors":"Shaghayegh T. Beheshti, M. J. Nazarideljou, M. Salehi","doi":"10.14720/aas.2023.119.2.2997","DOIUrl":"https://doi.org/10.14720/aas.2023.119.2.2997","url":null,"abstract":"The purpose of this experiment is to compare the growth and water consumption efficiency of five garden plants (marigold (Tagetes erecta ‘Red Brocade’), moss-rose (Portulaca grandiflora ‘Sun Rose’), dahlia (Dahlia sp. ‘Double Opra’), gazania (Gazania splendens ‘New Day’), and Indian blanket (Gaillardia pulchella. ‘Sun Dance’)) during the warmer seasons of the year under various levels of drought stress based on field capacity (FC; 25, 50, 75, and 100 %). The interaction effect of plant × drought stress (FC) on the fresh and dry mass of aerial and underground organs was significant. Decreased water availability resulted in a drop in growth parameters (leaf fresh and dry mass and leaf area). In compared to the growth of aerial organs, root biomass increased in response to drought stress. Marigold, Indian blanket, and dahlia plants had the highest root-to-shoot ratio in extreme stress, i.e., FC 25 %. The plant × drought stress interaction significantly influenced flower number, whereas flower diameter was influenced by the main effect of plant and drought stress (not their interaction). The FC 100 % and FC 25 % treatments had the highest and the lowest accumulations of proline and soluble sugars, respectively. Moss-rose, gazania, and marigold ornamental plants had the highest water use efficiency at 75 %, followed by Dahlia at 50 % and moss-rose at 25 %.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"99 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77353963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Zaki, Mona Mohamed Mansour, Samah O. Osman, Nagwa R. A. Hussein
{"title":"Variability of genetic - morphological traits of eleven seed strains of Mangifera indica L. growing in Upper Egypt","authors":"H. Zaki, Mona Mohamed Mansour, Samah O. Osman, Nagwa R. A. Hussein","doi":"10.14720/aas.2023.119.2.2949","DOIUrl":"https://doi.org/10.14720/aas.2023.119.2.2949","url":null,"abstract":"Mango (Mangifera indica L.) is one of the tastiest fruits in the world, with numerous advantages beyond their economic value. Eleven genotypes of mango various cultivars were examined for variability, heritability, and genetic advance, as well as multivariate analysis based on cluster and principal component analysis (PCA) for yield and some of its contributing traits during the two growing seasons, 2021 and 2022. All studied traits showed significant differences, and the phenotypic coefficients of variation (PCV) were found to be higher than genotypic coefficients of variation (GCV), supporting the idea that morphological (genetic) traits are more prevalent than environmental influence. All traits had substantial heritability ranging from 75.63 to 99.93 %, and the highest significant genetic advance (119.09 %) was for the number of fruits per tree than other traits. Four clusters were formed, i.e., clusters I and IV had four genotypes, cluster II had two, and cluster III had one genotype. The highest cluster mean values for fruit diameter, fruit mass, yield per tree, and the number of fruits per tree were found in Cluster II, followed by cluster I. Greater genetic divergence was found between ‘Zebda’ or ‘S9’ or ‘S10’ with most other genotypes, indicating that these genotypes may be used to study the characters’ broad range of variability and to yield high-quality recombinant lines. In light of the fact that mango is a very heterozygous crop, our current genetic results can be used for the selection of the appropriate parents in hybridization programs and in vegetative propagation to yield selective traits.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81081046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Peroxidase activity as a biochemical marker of insecticide use in vegetables","authors":"Nassima Senani, Samia Bedouhène, K. Houali","doi":"10.14720/aas.2023.119.2.2657","DOIUrl":"https://doi.org/10.14720/aas.2023.119.2.2657","url":null,"abstract":"The insecticides use is important for crop improvement and protection, but in excessive amounts, they would induce a dysfunction of metabolic enzymatic systems in plant tissues, leading to undesirable qualitative changes. In this context, we are interested in peroxidase (POD), an important enzyme in plant physiology but whose activity seems to be conditioned by the presence of insecticides in the soil. This work aims to study the impact of locally used insecticides (chlorpyrifos and dimethoate) on the activity of POD in parsley, onion, celery and garlic grown in soils treated or not. POD extraction was performed using Tris-HCl buffer (pH 7.3); its activity was measured using the substrate o-dianisidine in the presence of H2O2. Our result showed that POD activity for insecticide treated parsley, celery and onions increased by 30 % 127 % and 341 % respectively, however did not change significantly for garlic. Thus, the action of these chemicals is not trivial because they may alter non-target pathways, especially when doses are not adjusted accordingly. We found that insecticide stress would increase POD activity in all vegetables except garlic, which showed tolerance to insecticides. Our findings suggest that organic farming conditions could minimize peroxidase activity in parsley, celery and onion. We add that overproduction of POD negatively affects the quality and reduces the shelf life of vegetables, thus would be a very interesting biomarker of insecticide stress.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86179024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization of nuclear DNA content and chromosome numbers of Tulipa luanica Millaku, T. kosovarica Kit Tan, Shuka & Krasniqi and T. albanica Kit Tan & Shuka","authors":"Mirsade Osmani, M. Tuna, I. Elezaj","doi":"10.14720/aas.2023.119.2.13280","DOIUrl":"https://doi.org/10.14720/aas.2023.119.2.13280","url":null,"abstract":"The Balkan Peninsula is considered an important centre of native tulip species. Tulipa kosovarica and Tulipa luanica are new species recently discovered in Kosovo, and Tulipa albanica in Albania. The current study aims at the investigating the nuclear DNA content and chromosome number of these three tulipa species in order to provide for the first time data on their genome size and differences among these three Tulipa species. Analysis of nuclear DNA content was performed by flow cytometer (Partec CyFlow Space) in mature fresh leaves for each Tulipa species. Samples for chromosome analysis were taken from the root tip meristem of the bulbs. Results showed significantly higher amounts of nuclear DNA (2C) in T. luanica compared to T. kosovarica and T. albanica. The chromosome number for these three species was 2n = 2x = 24, while the chromosome sizes of T. luanica resulted larger, compared to that of T. kosovarica and T. albanica. A correlation between the nuclear DNA content and chromosome size was found among these tulipa species. Moreover, nuclear DNA content and chromosome sizes of T. luanica, T. kosovarica and T. albanica showed clear differences among these species.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"89 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83452717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yu. V. Gavrilyuk, I. Aksyonov, Nataliya Matsay, A. Beseda, I. Aksyonova
{"title":"Dissemination of the quarantine weeds of the genus Ambrosia in the steppe zone of Ukraine","authors":"Yu. V. Gavrilyuk, I. Aksyonov, Nataliya Matsay, A. Beseda, I. Aksyonova","doi":"10.14720/aas.2023.119.2.2492","DOIUrl":"https://doi.org/10.14720/aas.2023.119.2.2492","url":null,"abstract":"The article presents the results of many years of research for the period 2003-2020, which were aimed at studying the dissemination and expansion of plant groups of quarantine species of Ambrosia weeds in the eastern steppe of Ukraine. It has been established that the intensive dissemination of the species of the quarantine ragweed weed occurs both naturally and anthropically. Monitoring of the dissemination and growth of ragweed species is noted in all types of phytocenoses of the steppe. The increase in the areas of dissemination of aggressive species of ragweed weeds poses a widespread threat to all plant populations. Studies show a tendency to increase the number of ragweed plants in such plant groups as agrophytocenoses, phytocenoses of planted forests, phytocenoses of urban areas, meadows and pastures. For the period 2011-2002 the number of ragweed plants increased in meadows by 7.5 times, in pastures by 14.8 times, in agrophytocenoses by 2.95 times, in phytocenoses of urban areas by 1.68 times, in phytocenoses of planted forests by 1.28 times. Due to the lack of control over the dissemination and appropriate eradication measures, the largest increase in the number of ragweed plants over the past 10 years has been observed in meadows and pastures. It is recommended in phytocenoses of planted forests and urban areas in meadows and pastures, total mowing of ragweed plants before their flowering 5-7 times during the growing season in order to prevent replenishment of the seed stock of this weed in the soil during the growing season.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81495844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mehdi Jamshideyni, M. Behdani, S. Parsa, S. Khoramdel
{"title":"Evaluation of yield and water use efficiency of quinoa under irrigation regimes, gamma aminobutyric acid, and vermicompost application","authors":"Mehdi Jamshideyni, M. Behdani, S. Parsa, S. Khoramdel","doi":"10.14720/aas.2023.119.1.2970","DOIUrl":"https://doi.org/10.14720/aas.2023.119.1.2970","url":null,"abstract":"The current study was aim to evaluate the interaction effects of gamma aminobutyric acid (GABA) and vermicompost on yield and yield components of quinoa under different levels of drought stress. For this, two experiments were similarly designed as the factorial-split with four replicates for evaluating this hypothesis. Irrigation regimes (50, 75, and 100 % of plant water requirement (PWR)) as the main plot and vermicompost V (0, 5 t ha-1) × gamma aminobutyric acid GABA levels (0, 5, 10 mg l-1) as the subplot were designed. Severe drought stress had a significant effect on plant height. Plant height reduced 31.8 % after using 50 % of PWR compared to the control conditions. Although drought stress negatively affected the 1000 seed mass and seed yield, GABA foliar application alleviated these effects. After using 50 % of PWR, 10 mg l-1 of GABA increased the seed yield and harvest index up to 21.22 and 15.5 %, respectively, compared to the non-foliar application. The reduction in PWR from 100 to 50 % led to increasing in P and K concentrations, as well as sugar and proline contents. In the same conditions, the use of GABA or V had a significant effect on improving these traits. A similar trend was also recorded in relation to water use efficiency. Therefore, using 10 mg l-1 of GABA and 5 t ha-1 of V can be effective in alleviating water stress.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84723465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sergey Сhetverikov, A. Feoktistova, M. Timergalin, T. Rameev, Gaisar Hkudaygulov, Aliya Kendjieva, M. Bakaeva, Darya Сhetverikova, Sergey Starikov, D. Sharipov
{"title":"Mitigation of the negative effect of auxinic herbicide by bacterial suspension of Pseudomonas protegens DA1.2 in wheat plants under drought conditions","authors":"Sergey Сhetverikov, A. Feoktistova, M. Timergalin, T. Rameev, Gaisar Hkudaygulov, Aliya Kendjieva, M. Bakaeva, Darya Сhetverikova, Sergey Starikov, D. Sharipov","doi":"10.14720/aas.2023.119.1.2764","DOIUrl":"https://doi.org/10.14720/aas.2023.119.1.2764","url":null,"abstract":"Effect of auxin-producing bacterial strain (Pseudomonas protegens DA1.2) was investigated under conditions of drought and herbicide treatment in wheat plants. Positive effect of the bacterial suspension on wheat plants treated with auxinic herbicide under drought conditions was manifested in reducing the content of malondialdehyde and proline, preventing inhibition of plant growth and normalizing chlorophyll content. Under combined stress, changes in concentrations and redistribution of phytohormones in plants were detected. An imbalance in auxin distribution between shoots and roots could be the reason for the decrease in plant resistance to drought in combination with the herbicide. Treatment of plants with the bacterial suspension restored normal shoot-to-root ratio of auxins in plants. Thus, this bacterial strain showed the properties of synthetic auxin antidotes and can be recommended for optimizing the technology of herbicide application under drought conditions.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84113393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shankar Marlabeedu, S. Mishra, H. Sharma, Jagadish Jaba, Ramesh Babu Tatineni, Sridevi Dasari
{"title":"Standardization of detached leaf assay to screen chickpeas for resistance to beet armyworm, Spodoptera exigua (Hübner, 1808)","authors":"Shankar Marlabeedu, S. Mishra, H. Sharma, Jagadish Jaba, Ramesh Babu Tatineni, Sridevi Dasari","doi":"10.14720/aas.2023.119.1.2682","DOIUrl":"https://doi.org/10.14720/aas.2023.119.1.2682","url":null,"abstract":"The beet armyworm, Spodoptera exigua (Hübner, 1808) is an important pest of several economically important crops, and recently emerged as a serious pest of chickpea in South Central India. We standardized a detached leaf assay technique to evaluate chickpea germplasm and segregating populations for resistance to this pest under laboratory conditions. Two chickpea genotypes ICCL 86111 and ICC 3137 grown under field and greenhouse conditions were used for the detached leaf assay at the vegetative and flowering stages. The terminal branches were infested with 5, 10, 15, and 20 neonate larvae of S. exigua. The test genotypes were also infested with 2, 4, 6 and 8 third-instar larvae at the podding stage. At the vegetative stage, ICCL 86111 suffered less damage than ICC 3137 across infestation levels. The differences in larval survival between the genotypes were significant, and larval survival was lower on ICCL 86111 than on ICC 3137 across infestation levels. The results suggested that infesting the chickpea terminal branches with 10–15 neonate larvae per branch at the vegetative stage or six third-instar larvae at the podding stage can be used to evaluate chickpea genotypes for resistance to S. exigua.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81197948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Sammama, Mohamed Najib Alfeddy, D. Hsissou, Mimoun El Kaoua
{"title":"Potential effect of intercropping in the control of weeds, diseases, and pests in a wheat-faba bean system","authors":"H. Sammama, Mohamed Najib Alfeddy, D. Hsissou, Mimoun El Kaoua","doi":"10.14720/aas.2023.119.1.2564","DOIUrl":"https://doi.org/10.14720/aas.2023.119.1.2564","url":null,"abstract":"Intercropping has proved to be a promising alternative in the biological control of biotic factors by reducing the excessive use of plant protection products that are harmful to the environment and human health. In this study, aimed to examinate the effect of intercropping systems on diseases, weeds and pests control in organic field experiments in Western Morocco. Two field experiments were conducted during 2017-2018 and 2018-2019. Three cropping regimes (monocropped wheat, monocropped faba bean, and intercropped wheat-faba bean) and three nitrogen levels N0 (0 kg N ha-1), N1 (50 kg N ha-1), and N2 (100 kg N ha-1) were evaluated. Compared with monocropping, intercropping (N0 level) reduced the incidence of stripe rust by 71–120 % and severity by 244–337 % in 1st and 2nd experiments respectively. In addition, the incidence of septoria was reduced by 236 % and severity by 276 %. Obviously, the intercrops significantly decreased the total weed biomass by more than 40 % in both experiments. Black aphid populations in faba bean were reduced by 80 %. In contrast, the nitrogen fertilizer increased the attack of diseases and black aphids. It is concluded that wheat-faba bean intercrops can be used as a method of reduction of inputs, reduction of environmental impacts of crops, and stability in the face of biotic factors.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81627663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}