{"title":"Transkripcijsko uravnavanje adipogeneze in vloga koaktivatorja PGC-1a","authors":"Tamara MILOŠEVIČ BERLIČ, Peter Dovč","doi":"10.14720/aas.2004.84.2.14744","DOIUrl":"https://doi.org/10.14720/aas.2004.84.2.14744","url":null,"abstract":"Adipogeneza je kompleksen proces, na katerega vpliva veliko število genetskih in okoljskih faktorjev. Ker je posledica prekomernega nalaganja maščob debelost, pogosto pa tudi pojav diabetesa tipa 2, postaja proučevanje molekulskih osnov adipogeneze vse bolj pomembno tudi z vidika zdravja človeka. V živalski proizvodnji proces tvorbe maščobnega tkiva pomembno vpliva na kakovost prirasta, preko porabe krme pa na ekonomičnost proizvodnje. Poleg leptina in leptinskega receptorja, ki odločilno uravnavata nalaganje maščob, je bil v zadnjem času odkrit tudi mehanizem uravnavanja tvorbe maščobnega tkiva, ki vključuje adaptivno termogenezo in biosintezo mitohondrijev. Pomembna regulatorja teh procesov sta PPARg in njegov koaktivator PGC-1a. V pričujočem prispevku opisujemo mehanizem uravnavanja adipogeneze in vlogo PPARg in PGC-1a v njem. Prikazana je tudi medvrstna primerjava aminokislinskih zaporedij PGC-1a in možnosti za razvoj farmacevtskih učinkovin, ki bi vplivale na izražanje PGC-1a.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"86 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138971393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of the alcohol content on sensory perception of the fruit spirits","authors":"Arman Šekerić, Milenko Blesić, Pakeza Drkenda, Nermina Spaho","doi":"10.14720/aas.2023.119.4.15939","DOIUrl":"https://doi.org/10.14720/aas.2023.119.4.15939","url":null,"abstract":"Fruit spirits must have an aroma of the raw material, which is balanced by ethanol. Since many aroma compounds are more soluble in ethanol than in water, ethanol is the most important carrier of aroma compounds. The alcohol concentration seems to be crucial for the sensory profile of spirits. Alcohol content of 40% vol is the standard alcoholic strength of fruit spirits. Regulations specify a minimum alcohol content of 37.5% vol. However, ethanol reduction can result in change in sensory profile of spirits. The aim of this research is to determine whether lowering the alcohol content of spirits may make them less acceptable to customers. On this occasion, 5 pairs of fruit spirits were sensory tested: pear, plum, apple, raspberry, and grape spirits, each with a commercial and reduced alcohol concentration to 37.5% vol. The results showed that customers can recognize the difference in alcohol content of fruit spirits and dilution to lower alcohol content led to decreasing aroma for all tastes fruit spirits. However, typicality and intensity of fruit odour and the overall note of the spirits, were very similar perceived for Williams, plum and grape spirits whereas apple and raspberry spirits showed better characteristic at higher alcohol content.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"52 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139004206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"rapid and efficient DNA extraction method from high oily content seeds:","authors":"Elham R. S. Soliman","doi":"10.14720/aas.2023.119.4.16094","DOIUrl":"https://doi.org/10.14720/aas.2023.119.4.16094","url":null,"abstract":"Ricinus communis seeds harbor high oil and polyphenolics contents that hinder DNA extraction. Here, a rapid and efficient protocol for isolating total DNA from R. communis seeds was developed. The current method implies the use of repeated cycles of freeze/heat shock for the seed tissue to lyse the cells. DNA isolated with this protocol was successfully used as a template for PCR amplification of the internal transcribed spacer (ITS) region of the rRNA encoding gene that widely used for molecular identification of different plant species. As far to our knowledge, this study is the first one that report the efficient use of freeze/heat shock repeated cycles for isolation of a high-quality DNA from plant cells. The current protocol would support the subsequent analysis for seed lot purity analysis.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"210 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139003467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of fluorescence spectroscopy as a field method in the determination of varietal differences after tomato harvesting","authors":"V. Masheva, Vanya Slavova","doi":"10.14720/aas.2023.119.4.12518","DOIUrl":"https://doi.org/10.14720/aas.2023.119.4.12518","url":null,"abstract":"The study’s purpose is to establish the application based on fluorescence spectroscopy as a field method in the determination of varietal differences after tomato harvesting. The tomato fruits will be compared to determine the spectral distribution due to the varietal differences of a particular genotype. This will allow the approach to be practiced non-invasively in the quality control of tomato production in unspecified rooms and outdoors.The experimental studies have been conducted locally at the Institute of Plant Genetic Resources “K. Malkov” - Sadovo for three varieties.The spectral installation for the generation of emission fluorescence spectra is mobile. In its adjustment (optical adjustment), a system engineering approach based on the classical principles of modern optoelectronics was applied. The results of the experiment can be used to optimize the time for the analysis of the varietal difference of tomato genotypes after harvesting, under uncontrolled conditions. This will support the process of determining the belonging of a specific аccession to a given variety (even for аccessions of unknown origin) when it is necessary to qualify a score of samples in a short time.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"176 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139006184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Investigation of the medium for barley (Hordeum vulgare L.) immature embryo culture","authors":"Abdullah Hassn Mohammed","doi":"10.14720/aas.2023.119.4.13264","DOIUrl":"https://doi.org/10.14720/aas.2023.119.4.13264","url":null,"abstract":"Germination of immature embryos of interspecific crosses in vitro is the most important problem in barley breeding programs. The effect of four media types (MS, ½ MS, B-5, ½ B-5 on immature embryo culture of nine barley varieties (Sameer, Buraq, Aksad, Shuaa, Arevat, Alwarkaa, Alhadher, Amel, and Rihan) was studied by using completely randomized design (C.R.D.) with six replicates. Analysis of variance showed highly significant effects due to varieties, media, and interaction for all studied characteristics, indicated high variation among varieties and media. Aksad variety was significantly superior to all varieties for all studied traits except root number (RN). Medium of ½ B-5 was significantly superior to all media for all studied traits. Aksad interactions with media except B-5 media gave full germination with highly significant superiority over all interactions. Aksad x ½ B-5 interaction showed a highly significant superiority for shoot length (SL) and RL. As a result, ½ B-5 medium was the efficient medium for germination of immature embryos of most varieties. These varieties could be used in breeding program of interspecific crosses with wild species by using ½ B-5 medium.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"39 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139003497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Razvoj kmetijske rabe na območju fotovoltaične elektrarne D3 ob pretočni akumulaciji HE Brežice","authors":"Jože Podgoršek","doi":"10.14720/aas.2023.119.4.15746","DOIUrl":"https://doi.org/10.14720/aas.2023.119.4.15746","url":null,"abstract":"Agrovoltaika kot način dvojne rabe kmetijskih zemljišč je predmet številnih raziskav in projektov znanstveno raziskovalnih inštitucij in investitorjev. Podjetje Hidroelektrarne na Spodnji Savi, d.o.o. želi na obstoječi deponiji s postavljeno sončno elektrarno ob pretočni akumulaciji hidroelektrarne v Brežicah razviti koncept kmetijske rabe kmetijskih zemljišč med in pod obstoječimi paneli fotovoltaične elektrarne. Sončna elektrarna je postavljena v občini Brežice, parcele so v k.o. Krška vas. Celotna površina, primerna za razvoj kmetijske rabe, na kateri v večjem delu stoji sončna elektrarna, je 8,8 hektarjev. S študijem primera smo pregledali teoretična izhodišča za načrtovanje kmetijske pridelave na območju obstoječe sončne elektrarne. S študijo smo izbrali primerne vrste in sorte rastlin, ki bi bile primerne za pridelavo na tem območju. Za dolgoročno finančno vzdržnost kmetijske pridelave smo v študiji preverili tudi pričakovane stroške in prihodke za načrtovano kmetijsko rabo zemljišč. Na podlagi študije primera ugotavljamo, da bi to površino lahko uporabili tudi za rastlinsko pridelavo, kot je pridelovanje špargljev, ribeza in malin. Ob zaključku raziskovalne naloge smo predlagali, da se v bodoče načrtuje fotovoltaika skupaj s kmetijsko rabo, saj je možnosti za dvojno rabo kmetijskih zemljišč ob sočasnem načrtovanju energetike s kmetijstvom bistveno več.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"141 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139006156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Khamis, Ali Abdulla Ali, Mwanamanga Haji Ali, Ali Seif Nassor, Mohammed Omar Hamad, Salma Omar Mohammed, Mohammed Dhamir Kombo
{"title":"Effectiveness of local biopesticides in the control of Diamondback Moth (Plutella xylostella L.) in cabbage production in Zanzibar, Tanzania","authors":"A. Khamis, Ali Abdulla Ali, Mwanamanga Haji Ali, Ali Seif Nassor, Mohammed Omar Hamad, Salma Omar Mohammed, Mohammed Dhamir Kombo","doi":"10.14720/aas.2023.119.4.16424","DOIUrl":"https://doi.org/10.14720/aas.2023.119.4.16424","url":null,"abstract":"This experiment was conducted to determine the effectiveness of different types of local biopesticides to control diamondback moth (DBM) in cabbage production in Zanzibar. The experiment was conducted in horticulture farms at Zanzibar Agricultural Research Institute. The experiment was conducted in a randomized complete block design, with 6 treatments and a control, each of which was replicated 3 times. The following plants were used as biopesticide which are garlic (T1), hot pepper (chilli) (T2), clove (T3), mixture of garlic and pepper (T4), mixture of garlic and clove (T5), synthetic insecticide (T6) and a control (T0). The parameters were evaluated by conducting initial data collection (before treatment) and final data collection (after treatment). The average number of leaves affected by DBM and yield in terms of average mass (kg) of cabbage were recorded. Results showed that T6 was significantly associated with a lowest average number of affected leaves and higher yield followed by T4 and T5. Overall, garlic-treated plots had recorded higher yield compared to the non-garlic treated plots. Therefore, garlic extract as local biopesticide can effectively repel DBM. This study recommends that garlic can be used as an alternative to using synthetic chemicals to control DBM in cabbage crop.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139003137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Influence of mulches on soil moisture and water infiltration in the tomato crop","authors":"Imerina TANKEUOO KOPA, Mohamed Meddi, Abdelhadi Ammari","doi":"10.14720/aas.2023.119.4.15748","DOIUrl":"https://doi.org/10.14720/aas.2023.119.4.15748","url":null,"abstract":"Soil moisture is a key parameter of soil monitoring for observation of vegetation growth, predicting crop production, and improving water resource management. In this study, the objective is to compare the evolution of soil moisture in different mulches to determine the best mulch and its characteristics of infiltration in the soil. The experiment was conducted during the summer season in July-September 2022 on four different mulches (wood chips, sawdust, straw, mixture), and control at the experimental plot of Blida. The results showed that silt is the main matrix of the soil. The analysis of infiltration data identified modified Kostiakov as the best model of the study site, whose period of plant growth represents the phase during which we have a better infiltration under the mixture. The application of the mulch changes the moisture mainly at 15 cm and the conductivity at 10 cm. In addition, the mixture is the best mulch to conserve moisture in the soil while reducing the frequency of irrigation. The correlation between soil moisture and conductivity was overall very good. This was due to the effect of mulch, soil texture, plant root development, and capillary rise.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"27 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139004924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Blaž Ferjančič, M. Korošec, Saša Piskernik, J. Bertoncelj
{"title":"Effects of particle size on determination of the contents of grain and legume dietary fibre and resistant starch","authors":"Blaž Ferjančič, M. Korošec, Saša Piskernik, J. Bertoncelj","doi":"10.14720/aas.2023.119.4.15383","DOIUrl":"https://doi.org/10.14720/aas.2023.119.4.15383","url":null,"abstract":"Dietary fibre comprises non˗digestible carbohydrates, including resistant starch, and lignin, and it is an important constituent of a healthy diet. The aim was to define the influence of particle size on contents determined for dietary fibre and resistant starch in unprocessed grain and canned legumes. Five samples of unprocessed and processed grains were analysed, as oatmeal, buckwheat, dehulled barley, wheat and spelt, and three canned legumes, as beans, chickpeas and peas, with and without their brine. Samples were initially milled unscreened, and then again through 500 μm or 350 μm screens. For unprocessed grain samples, there was generally no influence of particle size, except for the 350-μm milling of dehulled barley, with significantly decreased contents determined for insoluble dietary fibre and resistant starch presumably due to damaging of starch granules and disrupting crystalline formation of starch. For canned legumes with and without their brine, particle size had little effect on contents determined for dietary fibre and resistant starch.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"11 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139005503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohamed M. Ibrahim, M. Ghoni̇my, Eid Abd El Rahman
{"title":"Physical and aerodynamic properties of date palm pollen grains","authors":"Mohamed M. Ibrahim, M. Ghoni̇my, Eid Abd El Rahman","doi":"10.14720/aas.2023.119.4.13421","DOIUrl":"https://doi.org/10.14720/aas.2023.119.4.13421","url":null,"abstract":"The present study was designed to determine the effect of four moisture content levels (4, 5, 6, and 7 %) on the physical and aerodynamic properties of date palm pollen grain (DPP). The physical properties of DPP included pollen length (L), width (w), thickness (T), projected area (Ap), geometric mean diameter (dg), mass (m), sphericity (S), and bulk density (ρp). It was observed that the moisture content did not significantly influence the physical properties of the DPP. The aerodynamic properties of DPP included the terminal velocity (Vt), drag coefficient (Dc), drag force (Df), and Reynolds number (Re). The pollen Reynolds number (Re) is significant at different pollen grain moisture content, and regression models were developed in the form of polynomial and exponential relationships. Also, the 3rd order polynomial relationship was found between Re and Dc. The results showed that the average values of Vt, Dc, Df, and Re were about 0.6 m s-1, (0.38 to 0.45), 1.09E-11 N, and (0.29 to 0.42), respectively. The results of this study will be helpful in the performance of date palm pollination machines.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"36 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139003919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}