生物材料与纳米技术(英文)最新文献_第7页

Cytotoxicity Study of Gold Nanoparticles on the Basal-Like Triple-Negative HCC-1937 Breast Cancer Cell Line 金纳米颗粒对基底样三阴性HCC-1937乳腺癌细胞株的细胞毒性研究

生物材料与纳米技术(英文) Pub Date : 2018-01-01 DOI: 10.4236/JBNB.2018.91002

C. Massard, Clémence Dubois, V. Raspal, Pierre Daumar, Y. Sibaud, E. Mounetou, M. Bamdad, O. Awitor

{"title":"Cytotoxicity Study of Gold Nanoparticles on the Basal-Like Triple-Negative HCC-1937 Breast Cancer Cell Line","authors":"C. Massard, Clémence Dubois, V. Raspal, Pierre Daumar, Y. Sibaud, E. Mounetou, M. Bamdad, O. Awitor","doi":"10.4236/JBNB.2018.91002","DOIUrl":"https://doi.org/10.4236/JBNB.2018.91002","url":null,"abstract":"The Triple Negative “Basal-like” breast cancer (TNBL) tumours have a high proliferative capacity and develop a resistance phenotype associated with metastases. However, the management of TNBL carcinomas is still not standardized. Among the promising trails, gold nanoparticles could be a relevant tool for the development of a targeted treatment for this breast cancer subtype in monotherapy, associated and/or conjugated with other drugs. In this work, we report the cytotoxicity impact of gold nanoparticles wrapped in Poly-Ethylene Glycol (PEG) on the TNBL HCC-1937 breast cancer cell line. PEG-coated gold nanoparticles (PEG-Au NPs) were synthesized by a two-step method using a reduction process followed by a post-functionalization called PEGylation. PEG-Au NPs were characterized using transmission electron microscopy and X-ray diffraction. The gold content of the samples was determined using atomic absorption spectrometer. The cytotoxicity tests were performed using Sulforhodamine B survival test and resazurin viability test. PEG-Au NPs impact analysis on HCC1937 TNBL cell line showed a clear toxic action of type dose dependent and at long term. These PEGylated gold nanoparticles present a promising tool for the development of tumor-specific radiosensitizing vectors, with or without the association of other treatment strategies.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70896531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Nanomaterials and Cell Interactions: A Review 纳米材料与细胞相互作用：综述

生物材料与纳米技术(英文) Pub Date : 2017-08-31 DOI: 10.4236/JBNB.2017.84015

T. C. Jackson, B. Patani, M. Israel

引用次数: 15

Low Releasing Mitomycin C Molecule Encapsulated with Chitosan Nanoparticles for Intravesical Installation 壳聚糖纳米粒子包埋低释放度丝裂霉素C分子用于膀胱内装置

生物材料与纳米技术(英文) Pub Date : 2017-08-31 DOI: 10.4236/JBNB.2017.84014

D. Kavaz, Feyza Kiraç, M. Kıraç, Ashok Vaseashta

{"title":"Low Releasing Mitomycin C Molecule Encapsulated with Chitosan Nanoparticles for Intravesical Installation","authors":"D. Kavaz, Feyza Kiraç, M. Kıraç, Ashok Vaseashta","doi":"10.4236/JBNB.2017.84014","DOIUrl":"https://doi.org/10.4236/JBNB.2017.84014","url":null,"abstract":"The aim of this investigation \u0000is preparation of Mitomycin-C encapsulated with chitosan nanoparticles synthesis using ionic gelation technique for \u0000intravesical controlled drug delivery \u0000systems. This study was conducted in \u0000vitro. Cumulative amount of drug released from the nanoparticles was \u0000calculated. Mitomycin-C release studies were examined for different pH values. \u0000During the drug loading and release studies, initial amount of drug was changed \u0000(i.e., 0.5, 1.25 and 2.5 mg) to get \u0000different release profiles and the release studies were repeated (n = 6). The loading efficiencies of \u0000Mitomycin-C with three different initial concentrations 0.5mg/ml, 1.25 mg/ml \u0000and 2.5 mg/ml into chitosan nanoparticles were 54.5%, 47.1% and 36.4%, respectively. \u0000For different pH values, the cumulative releases of Mitomycin-C from chitosan \u0000nanoparticles were 47% and 53% for pH 6.0 and 7.4, respectively (p p san nanoparticles \u0000was measured in T24 bladder cancer cell line in vitro, and the results \u0000revealed that the 2.5 MMC coated Chitosan nanoparticles had better tumor \u0000cells decline activity. From this investigation, we conclude that the drug encapsulated synthesized chitosan nanoparticles \u0000possess a high ability to be used as pH and dose responsive drug \u0000delivery system. This systematic investigation demonstrates a promising future \u0000for the intravesical installation in treatment of the superficial bladder \u0000cancer.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47777636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Functionalized Asymmetric Poly (Lactic Acid)/Gelatin Composite Membrane for Guided Periodontal Tissue Regeneration 功能化不对称聚乳酸/明胶复合膜引导牙周组织再生

生物材料与纳米技术(英文) Pub Date : 2017-08-31 DOI: 10.4236/JBNB.2017.84016

Shuangying Gu, Baoxiang Tian, Weicong Chen, Yue Zhou

{"title":"Functionalized Asymmetric Poly (Lactic Acid)/Gelatin Composite Membrane for Guided Periodontal Tissue Regeneration","authors":"Shuangying Gu, Baoxiang Tian, Weicong Chen, Yue Zhou","doi":"10.4236/JBNB.2017.84016","DOIUrl":"https://doi.org/10.4236/JBNB.2017.84016","url":null,"abstract":"Aim: Periodontitis is \u0000caused by chronic gingival inflammation and affects a large population in the world. Although guided tissue regeneration (GTR) therapy has been proven to be an effective treatment, the deficiency in the \u0000symmetrical design of all the GTR membrane \u0000in the market leaves large space for improvement. Therefore, we designed a novel asymmetrical bi-layer \u0000PLA/gelatin composite membrane for treating periodontitis. Methods: The PLA side was fabricated by electrospinning with \u0000metronidazole (MNA) pre-mixed with the PLA solution. The gelatin side \u0000containing bioglass (BG) 45S5 was fabricated with freeze-drying \u0000process and cross-linked with PLA membrane. The bio-compatibility of the \u0000membrane was evaluated in vitro using \u0000NIH3T3 cells. The releasing of MNA was measured by spectrophotometer. The \u0000bioactivity of the membrane was evaluated by hydroxyapatite (HA) deposit and determined by FTIR spectrometer. The ionic concentration of Ca2+ and was measured by ICPOES. The expression of the osteogenesis makers was determined by \u0000qRT-PCR. Results: The bi-layer PLA/gelatin composite membrane is \u0000biocompatible and bioactive. The releasing of MNA can rapidly reach the anti-bacterial effective concentration. \u0000Interestingly, the incorporation of MNA modulated the degradation rate of PLA scaffold to meet the requirement \u0000of tissue regeneration. Meanwhile, the embedding of the BG powder in the gelatin porous layer provided a favorable Ca2+ and ion environment for the regeneration of the alveolar bone \u0000tissue. Conclusions: Taken together, this bi-layer GTR membrane \u0000is closer to the physiological structure of the periodontal. The addition of \u0000MNA and BG makes it more powerful in treating periodontitis. Moreover, this \u0000research provides an example of biomimetic design in fabricating biomaterial \u0000for clinical applications.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48982933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Microtopography Attenuates Endothelial Cell Proliferation by Regulating MicroRNAs 微地形图通过调节微小RNA抑制内皮细胞增殖

生物材料与纳米技术(英文) Pub Date : 2017-07-13 DOI: 10.4236/JBNB.2017.83013

Dan Wang, Mengya Liu, Shuangying Gu, Yue Zhou, Song Li

{"title":"Microtopography Attenuates Endothelial Cell Proliferation by Regulating MicroRNAs","authors":"Dan Wang, Mengya Liu, Shuangying Gu, Yue Zhou, Song Li","doi":"10.4236/JBNB.2017.83013","DOIUrl":"https://doi.org/10.4236/JBNB.2017.83013","url":null,"abstract":"Endothelial cell (EC) morphology can be regulated by \u0000the micro/nano topography in engineered vascular grafts and by hemodynamic \u0000forces in the native blood vessels. However, how EC morphology affects miRNA \u0000and thus EC functions is not well understood. In this study, we addressed this \u0000question by using human umbilical vein endothelial cells (HUVECs) cultured on \u0000microgrooves as a model. HUVECs were grown on either microgrooved (with 10 μm \u0000width/spacing and 3 μm depth) or smooth surfaces. HUVECs on microgrooved \u0000surface had elongated and bipolar morphology, while HUVECs on smooth surface \u0000showed cobble stone shape or non-polar morphology. EdU staining indicated that \u0000HUVECs with elongated morphology had lower proliferation rate compared to their \u0000counterpart cultured on smooth surface. Quantitative PCR analysis demonstrated \u0000that the expression of the specific microRNAs (miR-10a, miR-19a, miR-221) that \u0000targeted proliferation-related genes was all up-regulated. Consistently, the \u0000mRNA levels of their respective target genes, mitogen-activated protein kinase \u0000kinase kinase 7, Cyclin D1 and c-kit were significantly reduced by a fold \u0000change of 0.12 ± 0.01 (p p 0.05) and 0.76 ± \u00000.21 (p < 0.05). Other miRNAs such \u0000as miR-126 and miR-181a were up-regulated as well, leading to the repression of \u0000their targets vascular cell adhesion molecule-1 and prospero homeobox-1. Our \u0000results suggested that microgrooved surface may regulate microRNA levels and \u0000thus EC functions. These results provide insight into the modulation of EC \u0000functions by microtopographic cues, and will facilitate the rational design of \u0000microstructured materials for cell and tissue engineering.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41320220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Facile Method for the Synthesis of Copper Nanoparticles Supported on the Organoclay Material 有机粘土材料负载纳米铜的简易合成方法

生物材料与纳米技术(英文) Pub Date : 2017-04-26 DOI: 10.4236/JBNB.2017.82010

M. Bambo, R. Krause, R. Moutloali

{"title":"Facile Method for the Synthesis of Copper Nanoparticles Supported on the Organoclay Material","authors":"M. Bambo, R. Krause, R. Moutloali","doi":"10.4236/JBNB.2017.82010","DOIUrl":"https://doi.org/10.4236/JBNB.2017.82010","url":null,"abstract":"In this study, synthesis of \u0000copper nanoparticles was performed using organoclay as a support to stabilize \u0000the nanoparticles. Organoclay amount was gradually increased, which had an \u0000effect on the morphology of the resultant nanoparticles. Low amount of \u0000organoclay added resulted in larger and agglomerated copper nanoparticles \u0000whereas increased amount of organoclay gave smaller sized nanoparticles. The \u0000hybrid materials were characterized using the SEM and TEM for morphology, XRD \u0000and FT-IR spectroscopy for structural elucidation, thermal analysis using TGA \u0000and also studying their antibacterial effect on the two well-known gram negative \u0000bacteria of E. coli and P. Aeruginosa. The synthesized \u0000nanoparticles were found to be crystalline Cu nanoparticles with a mix of CuO. \u0000Larger sized copper nanoparticles and agglomerates showed the higher thermal \u0000behaviour as compared with smaller nanoparticles with higher organoclay \u0000loading. The hybrid showed an improved antibacterial activity as compared with \u0000organoclay alone. The hybrid showed the higher antibacterial effect against the P. aeruginosa microorganism as \u0000compared with the E. coli microorganism.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46511910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

RETRACTED: Bioinspired Synthesis of Zinc Oxide Nanoparticle and its Combined Efficacy with Different Antibiotics against Multidrug Resistant Bacteria 生物启发合成氧化锌纳米颗粒及其与不同抗生素联合治疗多重耐药细菌的疗效

生物材料与纳米技术(英文) Pub Date : 2017-04-26 DOI: 10.4236/JBNB.2017.82011

S. Ehsan, M. Sajjad

引用次数: 18

Homogenized Porcine Extracellular Matrix Derived Injectable Tissue Construct with Gold Nanoparticles for Musculoskeletal Tissue Engineering Applications 均质猪细胞外基质衍生的可注射组织结构与金纳米颗粒在肌肉骨骼组织工程中的应用

生物材料与纳米技术(英文) Pub Date : 2017-04-26 DOI: 10.4236/JBNB.2017.82009

Sarah E. Smith, Colten Snider, D. Gilley, Daniel N. Grant, S. Sherman, B. Ulery, D. Grant, S. Grant

{"title":"Homogenized Porcine Extracellular Matrix Derived Injectable Tissue Construct with Gold Nanoparticles for Musculoskeletal Tissue Engineering Applications","authors":"Sarah E. Smith, Colten Snider, D. Gilley, Daniel N. Grant, S. Sherman, B. Ulery, D. Grant, S. Grant","doi":"10.4236/JBNB.2017.82009","DOIUrl":"https://doi.org/10.4236/JBNB.2017.82009","url":null,"abstract":"A unique porcine extracellular matrix (ECM) derived \u0000injectable tissue construct with 100 nm or 20 nm gold nanoparticles (AuNP) was \u0000developed for musculoskeletal tissue engineering applications. ECM has been \u0000shown to encourage cellularity and tissue remodeling due to its release of \u0000growth factors while AuNP have been shown to reduce reactive oxygen species \u0000(ROS) levels. Injectable tissue constructs were created by homogenizing \u0000decellularized porcine diaphragm tendon conjugated with 100 nm or 20 nm AuNP at \u00001x, 4x, and 8x concentrations. Extrusion force testing demonstrated that \u0000homogenized tissue constructs were injectable at an appropriate cannula size \u0000and force. L-929 murine fibroblasts were used to measure cell viability, cell \u0000proliferation, intracellular ROS levels, and cell migration in response to \u0000constructs. Enhanced cell viability and proliferation are observed on 1 × 20 nm \u0000AuNP constructs. ROS assays demonstrate reduced cellular ROS concentrations \u0000from all 20 nm AuNP constructs and from 8 × 100 nm AuNP constructs compared with \u0000constructs without nanoparticles. Cellular migration is higher towards 4 × 20 nm \u0000AuNP constructs compared with constructs \u0000without nanoparticles. Results support the potential use of a porcine ECM \u0000derived injectable tissue construct with AuNP as an injectable tissue construct \u0000to reduce inflammation and to promote tissue remodeling in musculoskeletal \u0000tissue engineering applications.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42005413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Surface Perfluoroalkyl Chains Segregation: A Tool for Reducing Calcium Deposits in Medical Grade Poly(Methyl Methacrylate) 表面全氟烷基链分离:减少医用级聚甲基丙烯酸甲酯中钙沉积的工具

生物材料与纳米技术(英文) Pub Date : 2017-04-26 DOI: 10.4236/JBNB.2017.82012

Baljit Ghatora, P. Foot, S. Barton, R. Thatti, A. Papagni, L. Vaghi

{"title":"Surface Perfluoroalkyl Chains Segregation: A Tool for Reducing Calcium Deposits in Medical Grade Poly(Methyl Methacrylate)","authors":"Baljit Ghatora, P. Foot, S. Barton, R. Thatti, A. Papagni, L. Vaghi","doi":"10.4236/JBNB.2017.82012","DOIUrl":"https://doi.org/10.4236/JBNB.2017.82012","url":null,"abstract":"Intraocular lenses can be manufactured from a wide variety of polymers, but due to the lost cost associated with the use of Poly(methyl methacrylate) (PMMA), it is still the preferred material used in the developing countries. However, a major drawback to its use is the build-up of calcium containing deposits that are formed on the intraocular lens over a period of time. In an attempt to hinder this deposition, surface modification of medical grade PMMA has been carried out using perfluoroalkyl chain (1,2,4-trifluoro-3-(C10F21CH2O)-7-(N,N)-dimethylaminoacridine) segregation. The segregation was explored using a 1% 1,2,4-trifluoro-3-(C10F21CH2O)-7-(N,N)-dimethyla-minoacridine in two methods: film casting and spin-coating, a thin film onto preformed PMMA discs. Both methods were compared against control PMMA to determine which method provided the best hindrance against calcium containing deposits when immersed in a simulated aqueous humour solution. Characterisation of the surface using scanning electron microscopy coupled with energy; dispersive x-ray analysis indicated that the surface segregation of perfluoroalkyl chains had hindered calcification in both methods. This pleminary research shows promising results of employing perfluoroalkyl chains in the surface segregation of biomaterials that can be employed in intraocular lenses.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43267402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Hydrothermal Liquefaction of Lignin 木质素的水热液化

生物材料与纳米技术(英文) Pub Date : 2017-01-20 DOI: 10.4236/JBNB.2017.81007

Julia Schuler, U. Hornung, A. Kruse, N. Dahmen, J. Sauer

引用次数: 36