生物材料与纳米技术(英文)最新文献_第10页

Feasibility of a Chronic Foreign Body Infection Model Studying the Influence of TiO2 Nanotube Layers on Bacterial Contamination 研究TiO2纳米管层对细菌污染影响的慢性异物感染模型的可行性

生物材料与纳米技术(英文) Pub Date : 2016-01-04 DOI: 10.4236/JBNB.2016.71006

C. Vorilhon, C. Massard, V. Raspal, Y. Sibaud, C. Forestier, N. Charbonnel, S. Descamps, K. Awitor

{"title":"Feasibility of a Chronic Foreign Body Infection Model Studying the Influence of TiO2 Nanotube Layers on Bacterial Contamination","authors":"C. Vorilhon, C. Massard, V. Raspal, Y. Sibaud, C. Forestier, N. Charbonnel, S. Descamps, K. Awitor","doi":"10.4236/JBNB.2016.71006","DOIUrl":"https://doi.org/10.4236/JBNB.2016.71006","url":null,"abstract":"Bacterial infections on the surface of medical devices are a significant problem in therapeutic approach, especially when implants are used in the living. In cardiology, pacemaker generator pocket surfaces, made in titanium alloy can be colonized by pathogen microorganism. This contamination represents a major risk of sepsis, endocarditis and localized infections for patients. A way to limit this bacterial contamination is to modify the surface topography using nano-structuration process of the titanium alloy surface of the implanted devices. The aim of this study is to evaluate the influence of TiO2 nanotube layers on bacterial infection in the living, considering the feasibility of an animal model of chronic foreign body infection. TiO2 nanotube layers prepared by electrochemical anodization of Ti foil in 0.4 wt% hydrofluoric acid solution were implanted subcutaneously in Wistar rats. Three weeks after implantation, TiO2 implants were contaminated by a Staphylococcus epidermilis strain using two different concentrations at 106 and 108 colony forming unit (CFU) in order to induce a sufficient infection level and to avoid unwanted over infection consequences on rats health during the experiments. After 28 days in the living, 75% of nanotube layers initially submitted to the 108 CFU inoculum were contaminated while only 25% nanotube layers initially submitted to the 106 CFU inoculum remained infected. This significant result underlines the influence of TiO2 nanotube layers in decreasing the infection level. Our in vitro experiments showed that the synthesized TiO2 nanotubes indeed decreased the Staphylococcus epidermilis adhesion compared to unanodized Ti foil.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70895583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Adjunction of Avidin to a Cysteamine Self-Assembled Monolayer for Impedimetric Immunosensor 亲和素与半胱胺自组装单分子膜的连接及其阻抗免疫传感器研究

生物材料与纳米技术(英文) Pub Date : 2016-01-04 DOI: 10.4236/JBNB.2016.71001

O. Ouerghi, M. Diouani, A. Belkacem, A. Elsanousi, N. Jaffrezic‐Renault

{"title":"Adjunction of Avidin to a Cysteamine Self-Assembled Monolayer for Impedimetric Immunosensor","authors":"O. Ouerghi, M. Diouani, A. Belkacem, A. Elsanousi, N. Jaffrezic‐Renault","doi":"10.4236/JBNB.2016.71001","DOIUrl":"https://doi.org/10.4236/JBNB.2016.71001","url":null,"abstract":"In this work an impedimetric immunosensor based on affinity immobilization method of a biotin labelled anti-human IgG antibody, used as a model system, was reported. The experimental procedure involves the growth of a self-assembled monolayer of a thiol (cysteamine) carrying terminal amine groups on gold electrodes. Glutarardehyde, a homobifunctional cross-linker, was used as a coupling reagent for the covalent linking of avidin to the amine groups of cysteamine. The attachment of the biotin labeled antibodies (anti-Human IgG) to the subsequent modified gold electrode was achieved by affinity interactions tacking advantage of the strong avidin-biotin bridge. The stepwise assembly process of the electrode was interrogated by means of cyclic voltammetry, electrochemical impedance spectroscopy and contact angle measurements. The response of the antibody modified electrode to their target antigens was investigated in the presence of BSA (bovin serum albumin) in order to alleviate non-specific adsorption problems. A proposed electrical model was used to analyse the experimental data. The resulting immunosensor has a linear dynamic range of 100 - 900 ng?ml–1 of antigen and a detection limit of 100 ng?ml–1.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70895446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Mechanosynthesis as a Simple Method to Obtain a Magnetic Composite (Activated Carbon/Fe3O4) for Hyperthermia Treatment 机械合成作为一种获得用于热疗的磁性复合材料(活性炭/Fe3O4)的简单方法

生物材料与纳米技术(英文) Pub Date : 2016-01-04 DOI: 10.4236/JBNB.2016.71003

J. C. Rios-Hurtado, E. Muzquiz-Ramos, A. Zugasti-Cruz, D. Cortés-Hernández

{"title":"Mechanosynthesis as a Simple Method to Obtain a Magnetic Composite (Activated Carbon/Fe3O4) for Hyperthermia Treatment","authors":"J. C. Rios-Hurtado, E. Muzquiz-Ramos, A. Zugasti-Cruz, D. Cortés-Hernández","doi":"10.4236/JBNB.2016.71003","DOIUrl":"https://doi.org/10.4236/JBNB.2016.71003","url":null,"abstract":"A large number of magnetic nanomaterials have been studied for their hyperthermic potential, such as iron oxide based materials. These are embedded in different matrices to improve their properties. In this paper magnetite was synthesized by the coprecipitation method and an activated carbon/magnetite composite was obtained by mechanosynthesis (400 rpm, 3 h). The samples were characterized by X-ray diffraction (XRD), vibrating sample magnetometer (VSM), IR-FT spectroscopy and Scanning Electron Microscopy (SEM). Furthermore, composite heating curves as well as hemolysis tests were performed. The composite showed a superparamagnetic behavior due to its low coercivity index (8.92 Oe) and a high saturation magnetization (40.12 emu/g). SEM images showed that the magnetite was observed on the surface of activated carbon and also the IR-FT spectra indicated that oxygenated groups on the activated carbon surface were responsible for the anchoring of magnetite in the surface, with particle sizes between 9 and 14 nm. Heating results indicated that a composite mass of 18 mg reach a temperature of 45.6&degC in a low frequency magnetic field (10.2 kA and 200 kHz). Hemolysis tests indicated that the composite is a non-hemolytic material (4.7% hemolysis). These results demonstrate that the material can be used in magnetic hyperthermia techniques for cancer treatment.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70895517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Optical Sum Frequency Generation Spectroscopy of Cracked Non-Glutinous Rice ( Oryza sativa L.) Kernels 干裂非糯米(Oryza sativa L.)的光学和频产生光谱内核

生物材料与纳米技术(英文) Pub Date : 2016-01-04 DOI: 10.4236/JBNB.2016.71002

Wataru Kouyama, T. Nishida, K. T. T. Hien, G. Mizutani, H. Hasegawa, Hiroaki Miyamura

引用次数: 1

Airborne Nanoparticles Filtration by Means of Cellulose Nanofibril Based Materials 纤维素纳米纤维基材料对空气中纳米颗粒的过滤

生物材料与纳米技术(英文) Pub Date : 2016-01-04 DOI: 10.4236/JBNB.2016.71004

L. Alexandrescu, K. Syverud, A. Nicosia, G. Santachiara, A. Fabrizi, F. Belosi

{"title":"Airborne Nanoparticles Filtration by Means of Cellulose Nanofibril Based Materials","authors":"L. Alexandrescu, K. Syverud, A. Nicosia, G. Santachiara, A. Fabrizi, F. Belosi","doi":"10.4236/JBNB.2016.71004","DOIUrl":"https://doi.org/10.4236/JBNB.2016.71004","url":null,"abstract":"Nanoparticles \u0000in air are of particular concern for public health and employee exposure in \u0000work-places. Therefore, it is very important to prepare effective filters for \u0000their removal. In this work filters were prepared from nanocellulose, \u0000i.e. cellulose nanofibrils (CNF). CNF was \u0000produced using two methods giving two different qualities of CNF. One quality \u0000had negative charges on the fibril surfaces while the other was neutral, and \u0000had in addition thinner fibrils compared to the other qualities. Filter samples \u0000were produced from water dispersions of CNF, by removal of the water by freeze \u0000drying. The performance of the CNF based filters was assessed and compared with \u0000filters based on synthetic polymer fibres. The ability to collect NaCl \u0000particles with a broad size distribution, ranging from nanometer to micrometer \u0000scale, was determined. CNF filters showed quality values comparable with the \u0000synthetic polymer based filters. Filters based on both the two CNF qualities \u0000had very good filtration efficiency for a given pressure drop across the \u0000filter.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70895532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Synthesis and Characterization of Fe3O4 Coated on APTES as Carriers for Morin-Anticancer Drug APTES包被Fe3O4作为桑酮抗癌药物载体的合成与表征

生物材料与纳米技术(英文) Pub Date : 2015-09-16 DOI: 10.4236/JBNB.2015.64025

B. Saif, Congli Wang, D. Chuan, S. Shuang

引用次数: 39

Physical properties of chitosan films obtained after neutralization of polycation by slow drip method 慢滴法中和多阳离子后壳聚糖膜的物理性质

生物材料与纳米技术(英文) Pub Date : 2015-09-16 DOI: 10.4236/JBNB.2015.64026

Jaiber H. R. Llanos, L. Vercik, A. Vercik

{"title":"Physical properties of chitosan films obtained after neutralization of polycation by slow drip method","authors":"Jaiber H. R. Llanos, L. Vercik, A. Vercik","doi":"10.4236/JBNB.2015.64026","DOIUrl":"https://doi.org/10.4236/JBNB.2015.64026","url":null,"abstract":"The development of new materials from biodegradable constituents is enticing for applications in bioelectronics and biomedicine, due to their considerable durability, versatility and biodegrada-bility. The present work aims to determine the chemical and physical properties of chitosan membranes after neutralization of the polycation solution. This work was divided into two stages: solubilization and neutralization. In the first stage, the chitosan was solubilized with three different concentrations of acetic acid (1%, 0.8% and 0.5%) in order to evaluate whether the acid affected the physical-chemical properties of the films. In the second stage, the chitosan polycation was neutralized with two strong bases (NaOH and KOH) using three different molar concentrations, by the slow drip method for obtaining films with pH values close to neutrality. The characterization of the membranes obtained from casting method showed that the different concentrations of acetic acid did not affect the physical-chemical properties of the films. In contrast, the neutralization process did affect their properties, and in particular, different behaviors were observed depending on the type of base used for neutralization. More ductile and plasticity were obtained in the films when the polycation was neutralized with KOH, whereas the films neutralized with NaOH exhibited a more fragile behavior.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70895387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Micro, Meso and Macro Porous Materials on Medicine 微、中、宏观多孔材料在医学上的应用

生物材料与纳米技术(英文) Pub Date : 2015-09-16 DOI: 10.4236/JBNB.2015.64023

Victor Solano-Umaña, J. Vega-baudrit

引用次数: 32

Natural ECM-Bacterial Cellulose Wound Healing—Dubai Study 天然ecm -细菌纤维素伤口愈合-迪拜研究

生物材料与纳米技术(英文) Pub Date : 2015-09-16 DOI: 10.4236/JBNB.2015.64022

Safwat Mohd. El-Hoseny, P. Basmaji, G. Olyveira, L. Costa, Abdulwahid Alwahedi, José C Oliveira, Gino Bruno Francozo

{"title":"Natural ECM-Bacterial Cellulose Wound Healing—Dubai Study","authors":"Safwat Mohd. El-Hoseny, P. Basmaji, G. Olyveira, L. Costa, Abdulwahid Alwahedi, José C Oliveira, Gino Bruno Francozo","doi":"10.4236/JBNB.2015.64022","DOIUrl":"https://doi.org/10.4236/JBNB.2015.64022","url":null,"abstract":"Bacterial cellulose (BC) can be used in wide area of applied scientific, especially for tissue regeneration and regenerative medicine, lately, bacterial cellulose mats are used in the treatment of skin conditions such as burns and ulcers, because of the morphology of fibrous biopolymers serving as a support for cell proliferation, its pores allow gas exchange between the organism and the environment. Moreover, the nanostructure and morphological similarities with collagen make BC attractive for cell immobilization, cell support and Natural Extracellular Matrix (ECM) Scaffolds. In this scope, Natural ECM is the ideal biological scaffold since it contains all the components of the tissue. The development of mimicking biomaterials and hybrid biomaterial can further advance directed cellular differentiation without specific induction. The extracellular matrix (ECM) contains several signals that are received by cell surface receptors and contribute to cell adhesion and cell fate which control cellular activities such as proliferation, migration and differentiation. As such, regenerative medicine studies often rely on mimicking the natural ECM to promote the formation of new tissue by host cells, and characterization of natural ECM components is vital for the development of new biomimetic approaches. In this work, the bacterial cellulose fermentation process is modified by the addition of vegetal stem cell to the culture medium and natural materials before the bacteria are inoculated. In vivo behavior using natural ECM for regenerative medicine is presented.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70895803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Cytotoxicity, in Vivo Skin Irritation and Acute Systemic Toxicity of the Mesoporous Magnesium Carbonate Upsalite® 介孔碳酸镁的细胞毒性、体内皮肤刺激和急性全身毒性

生物材料与纳米技术(英文) Pub Date : 2015-09-16 DOI: 10.4236/JBNB.2015.64024

S. Frykstrand, J. Forsgren, Peng Zhang, M. Strømme, N. Ferraz

{"title":"Cytotoxicity, in Vivo Skin Irritation and Acute Systemic Toxicity of the Mesoporous Magnesium Carbonate Upsalite®","authors":"S. Frykstrand, J. Forsgren, Peng Zhang, M. Strømme, N. Ferraz","doi":"10.4236/JBNB.2015.64024","DOIUrl":"https://doi.org/10.4236/JBNB.2015.64024","url":null,"abstract":"Upsalite® is a mesoporous magnesium carbonate synthesized without using surfactants and therefore highly attractive from environmental and production economy points of view. The material has recently been suggested as drug delivery vehicle and as topical bacteriostatic agent. In order to continue exploring these and other bio-related applications of the material, primary biocompatibility studies are needed. Herein we present the first in vivo acute systemic toxicity and skin irritation analyses as well as in vitro cytotoxicity evaluations of Upsalite®. The material was found to be non-toxic for human dermal fibroblasts cells up to a concentration of 1000 μg/ml and 48 h exposure in contrast to the mesoporous silica material SBA-15, used as reference, which significantly affected cell viability at particle concentration of 500 and 1000 μg/ml after the same exposure time. Topical application of Upsalite® resulted in negligible cutaneous reactions in a rabbit skin irritation model and no evidence of significant systemic toxicity was found when saline extracts of Upsalite® were injected in mice. Injection of sesame oil extract, however, resulted in transient weight loss, most likely due to injection of particles, and not toxic leachables. The presented results form the basis for future development of Upsalite® and similar mesoporous materials in biomedical applications and further toxicity as well as biocompatibility studies should be directed towards specific areas of use.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70895680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20