中华手外科杂志Pub Date : 2020-02-10DOI: 10.3760/CMA.J.ISSN.1005-054X.2020.01.008
Hao Su, G. Jin, Y. Le, J. Ju, Guangliang Zhang, Ping Zhang, Linfeng Tang, R. Hou
{"title":"A preliminary study on the method of single micro extrusion-based 3D printing of skin tissue","authors":"Hao Su, G. Jin, Y. Le, J. Ju, Guangliang Zhang, Ping Zhang, Linfeng Tang, R. Hou","doi":"10.3760/CMA.J.ISSN.1005-054X.2020.01.008","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-054X.2020.01.008","url":null,"abstract":"Objective \u0000To explore the feasibility of single micro extrusion 3D printing method for skin tissue. \u0000 \u0000 \u0000Methods \u0000Fibroblasts were isolated and cultured from human dermis and identified by immunofluorescence staining. Fibroblasts and HaCaT cells were used as seed cells. Gelatin, hyaluronic acid and fibrinogen were used to form scaffolds. HE staining and immunofluorescence staining were used to observe the cell distribution of 3D printed skin. The viability of cells in 3D printed skin tissue was detected by living/dead cell staining kit. Toluidine blue penetration assay was used to detect the barrier function of printed skin. \u0000 \u0000 \u0000Results \u0000More than 98% of the cultured fibroblasts isolated from skin dermis were vimentin positive. When the concentration of fibrinogen in the dermis and epidermis was 5 mg/ml and 1 mg/ml respectively, fibroblasts (4×106 cells/ml) printed 8 layers, and HaCaT cells (5×106 cells/ml) printed 1 layer, the prepared 3D printed skin tissue was cultured in air-liquid interface for 7 days and HE staining and immunofluorescence detection showed that the epidermis and dermis were well stratified with the thickness of epidermis being (63.5±3.5) μm. The viability of cells in 3D printed skin tissue was (90.2±0.9)% and (95.3±0.8)%, respectively, after submerge culture for 2 days and air-liquid interface culture for 7 days. After air-liquid interface culture for 7 days, the 3D printed skin tissue had similar barrier function to normal skin. \u0000 \u0000 \u0000Conclusion \u0000We have established a single micro extrusion 3D printing method for skin tissue, and successfully prepared tissue-engineered skin with structure, epidermis thickness and barrier function similar to normal human skin tissue, which may become an effective method for mass production of tissue-engineered skin in the future. \u0000 \u0000 \u0000Key words: \u0000Skin; Fibroblasts; HaCaT cells; Micro extrusion printing; Tissue structure","PeriodicalId":67383,"journal":{"name":"中华手外科杂志","volume":"36 1","pages":"28-32"},"PeriodicalIF":0.0,"publicationDate":"2020-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46729265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
中华手外科杂志Pub Date : 2020-02-10DOI: 10.3760/CMA.J.ISSN.1005-054X.2020.01.005
Fei Yin, Jun Wang, Xiao-fang Shen, C. Zou, Shuai Liu, Weifeng Lin
{"title":"Closed percutaneous reduction by leverage and Kirschner wire fixation for treatment of irreducible phalangeal neck fractures in children","authors":"Fei Yin, Jun Wang, Xiao-fang Shen, C. Zou, Shuai Liu, Weifeng Lin","doi":"10.3760/CMA.J.ISSN.1005-054X.2020.01.005","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-054X.2020.01.005","url":null,"abstract":"Objective \u0000To investigate the clinical efficacy of closed percutaneous reduction by leverage and Kirschner wire fixation in the treatment of irreducible phalangeal neck fractures in children. \u0000 \u0000 \u0000Methods \u0000From January 2016 to March 2019, 12 children with irreducible phalangeal neck fractures ware treated. The time from injury to operation was 1 to 46 days, including 5 cases of old fracture. Closed Kirschner wire puncture was used to clear callus and closed percutaneous reduction by leverage and Kirschner wire fixation were performed. The fracture healing time of the children was followed up after operation. Total active motion (TAM) system was used to evaluate the recovery of finger function. \u0000 \u0000 \u0000Results \u0000All the operations were completed successfully, and no infection, skin necrosis and fracture malunion occurred. All the children were follow-up for 6 to 36 months, with an average of 15.3 months. The fracture healing time was (4.2±0.2) weeks. The Kirschner wire was removed and functional exercises were performed 4 weeks after operation. The finger joint function was evaluated as excellent according to TAM system. \u0000 \u0000 \u0000Conclusion \u0000Closed percutaneous reduction by leverage and Kirschner wire fixation in the treatment of irreducible phalangeal neck fractures in children has the advantages of simple operation, less trauma and definite curative effect. It is necessary to strictly grasp the operative indications for old fracture. \u0000 \u0000 \u0000Key words: \u0000Finger phalanges; Fractures,bone; Fracture fixation; Kirschner wire","PeriodicalId":67383,"journal":{"name":"中华手外科杂志","volume":"36 1","pages":"20-22"},"PeriodicalIF":0.0,"publicationDate":"2020-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45896387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
中华手外科杂志Pub Date : 2020-02-10DOI: 10.3760/CMA.J.ISSN.1005-054X.2020.01.019
Rongyuan Yang, Jinhong Chen, Honghan Li
{"title":"Experimental study on the effect of green tea polyphenols combined with Mecobalamin on the repair of peripheral nerve injury","authors":"Rongyuan Yang, Jinhong Chen, Honghan Li","doi":"10.3760/CMA.J.ISSN.1005-054X.2020.01.019","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-054X.2020.01.019","url":null,"abstract":"Objective \u0000To investigate the effect of green tea polyphenols combined with Mecobalamin on nerve regeneration after sciatic nerve injury and repair. \u0000 \u0000 \u0000Methods \u0000Sixty adult Wistar rats were randomly divided into two groups, the experimental group and the control group, 30 rats in each group. Another 6 Wistar rats were taken as the blank control group. The experimental group was given green tea polyphenols 50 mg/(kg·d) and Mecobalamin 50 μ g/(kg·d) by gavage, while the control group was given Mecobalamin 50 μ g/(kg·d) by gavage for 2 weeks. Then the general condition, electrophysiological recovery, wet weight recovery rate of triceps surae muscle, the number of myelinated nerve fibers and the difference of histopathology were observed. \u0000 \u0000 \u0000Results \u0000The average nerve conduction velocity and myelinated nerve fiber count in the experimental group were significantly higher than those in the control group at the 2nd, 4th and 6th week after operation (P 0.05), and there was no significant difference between the experimental group, the control group and the blank control group at the 12th week after operation (P>0.05). The recovery rate of wet weight of triceps surae muscle in experimental group was significantly higher than that in control group (P<0.05). In the experimental group, the regenerated myelinated nerve fibers were evenly distributed, arranged relatively orderly, with high density and less hyperplasia of connective tissue. \u0000 \u0000 \u0000Conclusion \u0000The green tea polyphenols may play an important role in promoting peripheral nerve regeneration. \u0000 \u0000 \u0000Key words: \u0000Peripheral nerve; Nerve regeneration; Green tea polyphenols; Mecobalamin","PeriodicalId":67383,"journal":{"name":"中华手外科杂志","volume":"36 1","pages":"61-65"},"PeriodicalIF":0.0,"publicationDate":"2020-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47101502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Treatment of old mallet finger by folding palmaris longus tendon and pulling out wire technique","authors":"Yujun Zhang, J. Ju, Q. Zhao, Xiang-jun Li, Benyuan Wang, Heyun Cheng","doi":"10.3760/CMA.J.ISSN.1005-054X.2020.01.016","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-054X.2020.01.016","url":null,"abstract":"Objective \u0000To investigate the clinical efficacy of folding palmaris longus tendon and pulling out wire technique in the treatment of old mallet finger. \u0000 \u0000 \u0000Methods \u0000From June 2010 to June 2017, 19 cases (19 fingers) of old mallet fingers with extensor tendon and insertion defects were treated. The palmaris longus tendon was dissociated and folded, and the extensor tendon was repaired and the extensor tendon insertion was reconstructed by pulling out wire technique. \u0000 \u0000 \u0000Results \u0000All the wounds achieved primary healing after operation. The follow-up time ranged from 3 to 18 months with an average of 6 months. According to the Patel evaluation system for mallet finger, the results were rated as excellent in 7 cases, good in 9 cases, fair in 2 cases and poor in 1 case. \u0000 \u0000 \u0000Conclusion \u0000The method of folding palmaris longus tendon and pulling out wire technique for the treatment of old mallet finger is simple in material, simple in operation, good recovery of finger function and satisfactory in clinical efficacy. \u0000 \u0000 \u0000Key words: \u0000Tendon injuries; Treatment outcome; Transplantation; Mallet finger","PeriodicalId":67383,"journal":{"name":"中华手外科杂志","volume":"36 1","pages":"52-54"},"PeriodicalIF":0.0,"publicationDate":"2020-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42453915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
中华手外科杂志Pub Date : 2020-02-10DOI: 10.3760/CMA.J.ISSN.1005-054X.2020.01.009
Xiang-jun Li, J. Ju, Q. Zhao, Benyuan Wang, Heyun Cheng, Mingwei Li, Kun Zou
{"title":"Clinical application of biological membrane for reconstruction of A2 pulleys in tenolysis","authors":"Xiang-jun Li, J. Ju, Q. Zhao, Benyuan Wang, Heyun Cheng, Mingwei Li, Kun Zou","doi":"10.3760/CMA.J.ISSN.1005-054X.2020.01.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-054X.2020.01.009","url":null,"abstract":"Objective \u0000To explore the surgical technique and clinical efficacy of biological membrane for reconstruction of A2 pulleys in flexor tenolysis. \u0000 \u0000 \u0000Methods \u0000From July 2015 to December 2016, 10 cases of digital flexor tendon adhesion were treated. When the pulley system could not be repaired in situ after flexor tenolysis, pulley base (firmly combined with the tissue around the tendon) and body were made from biological membrane to be used for reconstruction of A2 pulleys. After the operation, functional exercises were performed under the protection of braces. \u0000 \u0000 \u0000Results \u0000All the wounds achieved primary healing after the operation. No foreign body reaction, abnormal exudation, redness, swelling, heat, pain, non-union or delayed union occurred. According to the evaluation criteria of Strickland and Glogovac, the results were graded as excellent in 4 fingers and good in 6 fingers within 48 hours after operation. The patients were follow-up form 6 to 18 months with the average of 11 months. According to the evaluation criteria of Strickland and Glogovac, the results were graded as excellent in 4 fingers and good in 6 fingers. No tendon rupture occurred again. The total flexion activity of fingers was improved, ranging from 135° to 260° with an average of 200°. No bowstring deformity occurred in the affected fingers after operation. \u0000 \u0000 \u0000Conclusion \u0000Biological membrane could be used for reconstruction of A2 pulley, which provides a new material for pulley reconstruction in tenolysis. It is a simple and effective method. \u0000 \u0000 \u0000Key words: \u0000Tendon injuries; Treatment outcome; Tenolysis; Pulley reconstruction; Biological membrane","PeriodicalId":67383,"journal":{"name":"中华手外科杂志","volume":"36 1","pages":"33-36"},"PeriodicalIF":0.0,"publicationDate":"2020-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44710925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
中华手外科杂志Pub Date : 2020-02-10DOI: 10.3760/CMA.J.ISSN.1005-054X.2020.01.013
Xiaochun Huang, Xiaobing Deng, Ying Wan
{"title":"Clinical efficacy of enhanced recovery after surgery nursing in patients with multiple severed fingers replantation","authors":"Xiaochun Huang, Xiaobing Deng, Ying Wan","doi":"10.3760/CMA.J.ISSN.1005-054X.2020.01.013","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-054X.2020.01.013","url":null,"abstract":"Objective \u0000To study the clinical efficacy of enhanced recovery after surgery nursing in patients with multiple severed fingers replantation. \u0000 \u0000 \u0000Methods \u0000From December 2016 to June 2018, 90 patients (232 fingers) with replantation of severed fingers were divided into experimental group and control group, 45 cases in each group. The experimental group was given enhanced recovery after surgery nursing and the control group was given routine nursing. The complications such as embolism and necrosis of replanted fingers in the two groups were analyzed, and nursing satisfaction was compared. \u0000 \u0000 \u0000Results \u0000The incidence of complications in the experimental group was 4.44%, which was lower than that in the control group of 17.78% with the difference being significant (P<0.05). The nursing satisfaction of the experimental group was 97.78%, which was higher than that of the control group of 84.44% with the difference being significant (P<0.05). \u0000 \u0000 \u0000Conclusion \u0000After the replantation of severed fingers, the nursing plan of enhanced recovery after surgery was used to intervene the patients, which can significantly reduce the risk of complications, promote the recovery of the condition, improve the prognosis effect, and improve the clinical nursing quality. \u0000 \u0000 \u0000Key words: \u0000Replantation; Postoperative complications; Enhanced recovery after surgery; Nursing care","PeriodicalId":67383,"journal":{"name":"中华手外科杂志","volume":"36 1","pages":"44-46"},"PeriodicalIF":0.0,"publicationDate":"2020-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49106966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The influence of three kinds of internal fixator on blood supply of scaphoid bone: a computer-aided study","authors":"Xulin Zhang, Yongqing Xu, Xiao‐qing He, Haotian Luo, Yujian Xu, Libo Yuan, Huan Wu, Wan-qiu Zhao","doi":"10.3760/CMA.J.ISSN.1005-054X.2020.01.014","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-054X.2020.01.014","url":null,"abstract":"Objective \u0000To study the effects of nickel-titanium memory alloy nail-feet-fixator, Herbert screw and Kirschner wire on blood supply in scaphoid bone using visual digital model of scaphoid with intraosseous vessels. \u0000 \u0000 \u0000Methods \u0000Twelve human forearm specimens were perfused with arterial blood vessels, scanned by micro-CT and reconstructed by Mimics 20.0 software. The reconstructed data were analyzed and compared with the digital models of three internal fixation devices. \u0000 \u0000 \u0000Results \u0000In this study, the visualized digital model of scaphoid with blood supply was combined with three kinds of internal fixation devices to reconstruct the composite model and construct the safe zone of scaphoid nutrient artery. Among them, nickel-titanium memory alloy nail-feet-fixator has the characteristics of less vascular interference. \u0000 \u0000 \u0000Conclusion \u0000The digital model can be used intuitively in the study of internal fixators. All three kinds of internal fixators are located in the safe zone of scaphoid nutrient vessels. Among them, nickel-titanium memory alloy nail-feet-fixator has less interference to internal vessels than Herbert screw and Kirschner wire. \u0000 \u0000 \u0000Key words: \u0000Scaphoid bone; Internal fixators; Vascular perfusion; Micro CT","PeriodicalId":67383,"journal":{"name":"中华手外科杂志","volume":"36 1","pages":"47-50"},"PeriodicalIF":0.0,"publicationDate":"2020-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41882125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Clinical application of TriGen interlocking intramedullary nail combined with suture anchor in the treatment of Neer type III and IV proximal humeral fractures","authors":"Yin Cui, Xiu-hui Wang, Minghui Wang, Beigang Fu, F. Gao, Wan-gen Guo","doi":"10.3760/CMA.J.ISSN.1005-054X.2020.01.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-054X.2020.01.004","url":null,"abstract":"Objective \u0000To analyze the clinical efficacy of TriGen interlocking intramedullary nail combined with suture anchor in the treatment of Neer typeⅢ andⅣ proximal humeral varus fractures. \u0000 \u0000 \u0000Methods \u0000From June 2015 to June 2017, 23 patients with proximal humeral varus fractures of Neer type Ⅲ and Ⅳ were treated with TriGen interlocking intramedullary nail combined with suture anchor technique, including 15 cases of Neer type Ⅲ and 8 cases of Neer type Ⅳ. The fracture healing time was followed up. The range of motion of shoulder joint was measured one year after operation. American Shoulder and Elbow Surgeons (ASES) score, Constant-Murley score and visual analogue scale (VAS) were performed. \u0000 \u0000 \u0000Results \u0000All the patients were follow-up for 12 to 16 months, with an average of 14 months. All fractures healed 2 to 4 months after operation, with an average of 3 months. No complications such as wound infection, rotator cuff injury and axillary nerve injury occurred. The ASES score, Constant-Murley score and VAS were (91.00±3.04), (90.00±3.21) and (0.42±0.38) respectively. \u0000 \u0000 \u0000Conclusion \u0000For Neer type Ⅲ and Ⅳ complex proximal humerus varus fractures, TriGen interlocking intramedullary nail combined with suture anchor technique is used to treat it, which increases the strength of fixing the humeral nodules, conforms to the concept of overall fixation, is conducive to early functional rehabilitation, and achieves good results after operation. It is a reliable minimally invasive treatment method. \u0000 \u0000 \u0000Key words: \u0000Humeral fractures; Surgical procedures, operative; Proximal; Intramedullary nail; Anchor","PeriodicalId":67383,"journal":{"name":"中华手外科杂志","volume":"36 1","pages":"16-19"},"PeriodicalIF":0.0,"publicationDate":"2020-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47022493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
中华手外科杂志Pub Date : 2020-02-10DOI: 10.3760/CMA.J.ISSN.1005-054X.2020.01.012
Wei Zhu, Xiaofeng Xu, F. Zhou, J. Ju, R. Hou
{"title":"Application of the first dorsal metacarpal reverse fasciocutaneous flap with the superficial branch of radial nerve for repair of thumb defect","authors":"Wei Zhu, Xiaofeng Xu, F. Zhou, J. Ju, R. Hou","doi":"10.3760/CMA.J.ISSN.1005-054X.2020.01.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-054X.2020.01.012","url":null,"abstract":"Objective \u0000To investigate the clinical efficacy of the first dorsal metacarpal reverse fasciocutaneous flap with the superficial branch of radial nerve for repair of thumb defect. \u0000 \u0000 \u0000Methods \u0000From January 2013 to June 2017, 14 patients with thumb defect were treated using the first dorsal metacarpal reverse fasciocutaneous flap with the superficial branch of radial nerve. The flap area was 2.0 cm×1.5 cm to 4.5 cm×3.5 cm. The donor area was sutured directly in 11 cases and covered with full thickness skin graft in 3 cases. \u0000 \u0000 \u0000Results \u0000All the 14 flaps were survived uneventfully without vascular crisis, necrosis and infection. The patients were follow-up for 6 to 15 months. The appearance of the fingers was aesthetic, the texture was soft, the skin color was close to the surrounding tissue, and the skin flap had good wear resistance. After the operation, the sensation of the flap recovered to S3+ with the two-point discrimination being 6 to 9 mm. The sensation of pain and temperature were recovered. Patients can use their thumbs for daily work and life. \u0000 \u0000 \u0000Conclusion \u0000The first dorsal metacarpal reverse fasciocutaneous flap with the superficial branch of radial nerve has the advantages of reasonable flap design, reliable blood supply, convenient cutting and simple operation. It is an ideal operation for repairing thumb defect. \u0000 \u0000 \u0000Key words: \u0000Surgical flaps; Treatment outcome; Thumb defect; Superficial branch of radial nerve","PeriodicalId":67383,"journal":{"name":"中华手外科杂志","volume":"36 1","pages":"41-43"},"PeriodicalIF":0.0,"publicationDate":"2020-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47151625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}