植物生理与分子生物学学报最新文献_第2页

Identification of drought-responsive genes from maize inbred lines. 玉米自交系干旱响应基因的鉴定。

植物生理与分子生物学学报 Pub Date : 2007-12-01

Fu-Hua Li, Feng-Ling Fu, Li-Na Sha, Wan-Chen Li

{"title":"Identification of drought-responsive genes from maize inbred lines.","authors":"Fu-Hua Li, Feng-Ling Fu, Li-Na Sha, Wan-Chen Li","doi":"","DOIUrl":"","url":null,"abstract":"To provide a useful piece of information for the choice of molecular markers to be used in selection of drought tolerance, mRNA differential display was used to isolate genes from a drought-tolerant maize inbred line '81565'. After drought stress, two down-regulated expression gene fragments (MD1 and MD2) and one up-regulated expression fragment (MD3) were obtained. Results of sequence and homology analysis show that MD1 has 97% similarity with matK in maize chloroplast genome, a gene encoding RNA maturase involved in group II intron splicing of RNA transcript; MD2 has 99% similarity with the gene serine/threonine phosphorylase 2C in Sporobolus stapfianus; and MD3 has 99% similarity with rice the gene encoding metacaspase, an arginine/lysine-specific cysteine protease. Based on the sequence of fragment MD2, a new member of maize PP2C gene family, ZmPP2Ca, was cloned by in silicon cloning and reverse transcription polymerase chain reaction (RT-PCR). Real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) showed that expression of the gene was down-regulated in the three drought-tolerant lines ('81565', 'N87-1' and 'R09') and up-regulated in the two drought-sensitive lines ('200B' and 'ES40') under drought stress.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27329145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning and expression of resistance gene analogs (RGAs) from wild banana resistant to banana Fusarium wilt. 野生香蕉抗香蕉枯萎病抗性基因类似物的克隆与表达。

植物生理与分子生物学学报 Pub Date : 2007-12-01

Ya-Ping Chen, Yun-Feng Chen, Jie-Tang Zhao, Xia Huang, Xue-Lin Huang

引用次数: 0

A rice blast-resistance genetic resource from wild rice in Yunnan, China. 云南野生水稻抗稻瘟病遗传资源研究。

植物生理与分子生物学学报 Pub Date : 2007-12-01

Ming-Zhi Yang, Zai-Quan Cheng, Shan-Na Chen, Jun Qian, Ling-Ling Xu, Xing-Qi Huang

{"title":"A rice blast-resistance genetic resource from wild rice in Yunnan, China.","authors":"Ming-Zhi Yang, Zai-Quan Cheng, Shan-Na Chen, Jun Qian, Ling-Ling Xu, Xing-Qi Huang","doi":"","DOIUrl":"","url":null,"abstract":"Compared to Pi-ta(-) alleles, Pi-ta(+) alleles can cause blast resistance response. In this work, Pi-ta gene in multiple rice materials, including local rice cultivars, different types of O. rufipogon and O. longistaminata was detected by molecular cloning and sequence analysis. Results indicated that Pi-ta(+) alleles were rare alleles, because in all the tested materials, only the 'Erect' type of O. rufipogon (ETOR) from Jinghong county in Yunnan province contains a Pi-ta(+) allele. Another rice blast resistance gene, Pib, confers resistance to the Japanese strain of M. grisea, was also confirmed to be functional in this type of O. rufipogon. The results of pathogen inoculation test show that ETOR is more strongly resistant to the tested blast pathogen races than other types of O. rufipogon. The resistance of ETOR may at least partially depend upon the functioning of Pi-ta and Pib gene. As O. rufipogon has the same type of genome with the cultivated rice (O. sativa), Pi-ta(+) and Pib gene in Erect type of O. rufipogon can be used to improve the tolerance of cultivated rice to blast, either by traditional hybridization or by genetic engineering.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27329143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repression of AtCLH1 expression results in a decrease in the ratio of chlorophyll a/b but doesnot affect the rate of chlorophyll degradation during leaf senescence. 抑制AtCLH1的表达导致叶绿素a/b比值降低，但不影响叶片衰老过程中叶绿素降解速率。

植物生理与分子生物学学报 Pub Date : 2007-12-01

Xiao Zhou, Yang Liao, Guo-Dong Ren, Yan-Yan Zhang, Wen-Jun Chen, Ben-Ke Kuai

{"title":"Repression of AtCLH1 expression results in a decrease in the ratio of chlorophyll a/b but doesnot affect the rate of chlorophyll degradation during leaf senescence.","authors":"Xiao Zhou, Yang Liao, Guo-Dong Ren, Yan-Yan Zhang, Wen-Jun Chen, Ben-Ke Kuai","doi":"","DOIUrl":"","url":null,"abstract":"To explore the possible regulatory role of chlorophyllase (Chlase) in chlorophyll (Chl) degradation during leaf senescence, RNAi Arabidopsis (Arabidopsis thaliana) plants were constructed to repress the expressions of AtCLH1 and/or AtCLH2. Transcript levels of AtCLH1 and/or AtCLH2 were dramatically lowered and Chlase activity was correspondingly inhibited, but the Chl degradation kinetics was not affected in the RNAi plants. Results of further analysis indicated that the Chl a/b ratio decreased in AtCLH1 RNAi lines, in comparison with the increasing Chl a/b ratio in the wide type during leaf senescence. In addition, an induced Chlase activity was consistently detected at the initial stage of senescence in all the plants examined. In contrast, transcript levels of both AtCLH1 and AtCLH2 decreased dramatically upon the initiation of senescence in both the wide-type and the RNAi plants. Interestingly, compared with the wide type, lower but still significant transcript levels of the RNAi targeted Chlase gene(s) were sustained during the whole period of dark incubation in all the three RNAi lines examined, indicating the functioning of some compensatively regulating mechanism. Based on these results, along with related reports, we conclude that Chlase might be required at the initial stage of leaf senescence, quite likely playing a role in converting Chl b to a.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27329144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Metabolic engineering of edible plant oils]. [食用植物油代谢工程]。

植物生理与分子生物学学报 Pub Date : 2007-12-01

Ai-Qin Yue, Xi-Ping Sun, Run-Zhi Li

{"title":"[Metabolic engineering of edible plant oils].","authors":"Ai-Qin Yue, Xi-Ping Sun, Run-Zhi Li","doi":"","DOIUrl":"","url":null,"abstract":"Plant seed oil is the major source of many fatty acids for human nutrition, and also one of industrial feedstocks. Recent advances in understanding of the basic biochemistry of seed oil biosynthesis, coupled with cloning of the genes encoding the enzymes involved in fatty acid modification and oil accumulation, have set the stage for the metabolic engineering of oilseed crops that produce \"designer\" plant seed oils with the improved nutritional values for human being. In this review we provide an overview of seed oil biosynthesis/regulation and highlight the key enzymatic steps that are targets for gene manipulation. The strategies of metabolic engineering of fatty acids in oilseeds, including overexpression or suppression of genes encoding single or multi-step biosynthetic pathways and assembling the complete pathway for the synthesis of long-chain polyunsaturated fatty acids (e.g. arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid) are described in detail. The current \"bottlenecks\" in using common oilseeds as \"bioreactors\" for commercial production of high-value fatty acids are analyzed. It is also discussed that the future research focuses of oilseed metabolic engineering and the prospects in creating renewable sources and promoting the sustainable development of human society and economy.","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27328188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Maize transformation using xylose isomerase gene as a selection marker]. [以木糖异构酶基因为选择标记的玉米转化]。

植物生理与分子生物学学报 Pub Date : 2007-12-01

Xin-Mei Guo, Xiao-Dong Zhang, Rong-Qi Liang, Li-Quan Zhang, Yao-Feng Chen

引用次数: 0

[The mechanisms of plant photosynthetic acclimation to elevated CO2 concentration]. [植物光合适应二氧化碳浓度升高的机制]。

植物生理与分子生物学学报 Pub Date : 2007-12-01

Dao-Yun Zhang, Da-Quan Xu

引用次数: 0

[Aluminum lightens the adverse effects of excessive Mn on growth of soybean (Glycine max)]. [铝能减轻过量锰对大豆（Glycine max）生长的不利影响]。

植物生理与分子生物学学报 Pub Date : 2007-12-01

Zhong-Bao Yang, Jiang-Feng You, Zhen-Ming Yang