{"title":"T Cell Apoptosis Was Inhibited by Fas of Antisense Oligonucleotide","authors":"Shi-ying Zheng, D. Jiang, J. Ge, Zhengjie Shen","doi":"10.1109/ICBBE.2010.5516974","DOIUrl":"https://doi.org/10.1109/ICBBE.2010.5516974","url":null,"abstract":"We investigate the effect of specific antisense oligodeoxynucleotide (ASO DN) inhibition of Fas expression on T cell apoptosis induced by gastric carcinoma cell . Fas receptor (Fas) and Fas ligand (FasL) expressed by the gastric carcinoma cell line SGC-7901 and Jurkat T cells were detected by flow cytometry (FCM) and the ability of FasL-inducing T cell apoptosis was tested by co-culture assay in vitro with SGC-7901 cells and Jurkat T cells. The Jurkat cells were transfected with Fas-ASODN using lipofectin, and the effects of Fas-ASODN on Fas mRNA level, Fas expression on T cells surface, and apoptosis were investigated by RT-PCR, FCM and co-culture assay, respectively. SGC-7901 cells expressing functional FasL could induce the apoptosis of Jurkat cells as demonstrated by co-culture assays. After the Jurkat cells were transfected with Fas ASODN, the level of Fas mRNA, the expression rate of Fas and the apoptotic rate induced by gastric carcinoma cells were all decreased. Gastric carcinoma cells expressing FasL can induce apoptosis in Fas-expressing T cells, indicating that transfection of Fas ASODN can partially convert the immune inhibitory condition induced by gastric carcinoma cells. Fas ASODN may be a useful tool in the armamentarium of tumor immune therapy.","PeriodicalId":6396,"journal":{"name":"2010 4th International Conference on Bioinformatics and Biomedical Engineering","volume":"135 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2010-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74290051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Microwave-Assisted COD Removal from Landfill Leachate by Hydrogen Peroxide, Peroxymonosulfate and Persulfate","authors":"Wenyi Zhang, Shiying Yang, Rui Niu, Xueting Shao, Liang Shan, Xin Yang, Ping Wang","doi":"10.1109/ICBBE.2010.5517536","DOIUrl":"https://doi.org/10.1109/ICBBE.2010.5517536","url":null,"abstract":"H2O2-based advanced oxidation processes (AOPs) and SO4•−-based AOPs were proved novel and promising techniques in treatment of wasterwater. Microwave (MW)-assisted hydrogen peroxide (H2O2), peroxymonosulfate (PMS) and persulfate (PS) were used to dispose of COD in landfill leachate. Oxidant dosage and chloride concentration were investigated as the major operating conditions. The initial COD is 4062.8 mg/L. It is demonstrated that MW-assisted H2O2, PMS and PS can effectively degrade leachate organics, and the COD removal can reach 43.5%, 80.2%, 97.3% when the oxidant concentration is 0.3 mol/L, respectively. Chloride ion will slightly improve the reaction rate for H2O2 and PS, while a little slow down for PMS, but all the eventual COD removal efficiencies keep stable. Generally speaking, MW-assisted peroxide is a powerful means in degradation of COD in landfill leachate.","PeriodicalId":6396,"journal":{"name":"2010 4th International Conference on Bioinformatics and Biomedical Engineering","volume":"2 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2010-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74402682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phosphates Recovery through Hydroxyapatite Crystallization from Wastewater Using Converter Slag as a Seed Crystal","authors":"J. Duan, Yin-lan Cao, Bi-yan He","doi":"10.1109/ICBBE.2010.5518291","DOIUrl":"https://doi.org/10.1109/ICBBE.2010.5518291","url":null,"abstract":"With powdered converter slag used as a seed crystal, the effects of slag dosage, pH, temperature, and Ca2+ concentration on phosphate removal from synthetic solution through crystallization were investigated in batch experiments. The results indicated that slag concentration of 1.7 g/L, pH value of 9-10, reaction time of 3h , reaction temperature of 20-25℃, Ca/P molar ratio of 2, seemed to be the favorable conditions. Under the optimal reaction condition, the removal efficiencies of the swine farm wastewater and the supernate of concentrated sludge , were 95.36% and 96.54%, respectively. After converter slag as a seed material was used for ten times, the P removal efficiency was still above 80%. The Scanning electron micrograph (SEM) images and X-ray diffraction (XRD) pattern of converter slag before and after phosphorus crystallization confirmed the formation of crystalline hydroxyapatite. Energy dispersive X-ray spectroscopy (EDS) mapping analysis revealed that the molar composition ratio of Ca/P of the crystalline material was 1.72. The Ca/P molar ratio > 1.67 for crystalline substance might result from the presence of CaCO3 on the crystalline surfaces. The results indicated that total P contents in the generated crystallization products of up to 14.5 % P-tot (33.2 % P2O5) were achieved, and was comparable to natural phosphate rock.","PeriodicalId":6396,"journal":{"name":"2010 4th International Conference on Bioinformatics and Biomedical Engineering","volume":"26 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2010-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74768474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Status and Prospects of Music Electrotherapy","authors":"H. Zhang, H. Hua","doi":"10.1109/ICBBE.2010.5514922","DOIUrl":"https://doi.org/10.1109/ICBBE.2010.5514922","url":null,"abstract":"Abstract-Music electrotherapy is on the basis of electro-acupuncture, which combined music,electrode with electro-acupuncture. This method draws on the characteristics of both electrotherapy and music therapy music. Music electrotherapy through music and music signals acting on the hearing and body, increase or improve the person's psychological, physiological conditions, so as to achieve health, disease prevention and rehabilitation purposes. In this article, some new ideas and methods based on programmable and distributed automatic system are proposed, in order to improve and perfect the existing music electrotherapy instruments.","PeriodicalId":6396,"journal":{"name":"2010 4th International Conference on Bioinformatics and Biomedical Engineering","volume":"306 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2010-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78914368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bei Guo, Y. Song, Xuefeng Guo, Wenping Wang, Ning Ding
{"title":"Inhibition of Gene Expression of Trehalase Enhances Drought Resistance in Transgenic Tobacco","authors":"Bei Guo, Y. Song, Xuefeng Guo, Wenping Wang, Ning Ding","doi":"10.1109/ICBBE.2010.5516954","DOIUrl":"https://doi.org/10.1109/ICBBE.2010.5516954","url":null,"abstract":"The representative individuals, CK (non-transgenic), 121 (with pBI121 vector), 1285 (with pGSA1285 vector), T4 (with gene of trehalase, TRE), S1 (with gene of trehalose -6 - phosphate synthase, TPS), P3 (with gene of trehalose -6 - phosphate phosphatase, TPP), a3 (with anti-TRE gene), i2 (with RNAi-TRE gene), were selected for identification of drought resistance, and the Malondialdehyde (MDA), relative conductivity have been measured after water-stress during this experiment. The results showed that the transgenic tobaccos with anti-TRE and RNAi-TRE grew better than other individuals under water-stress conditions; conductivity of i2 was lower than other plants. Meantime, i2 has performance of some special features. These indicate that through application the antisense RNA and RNA interference technology to trehalase gene, it is possible to make the receptor trehalose metabolism changes. The inhibition of gene expression of trehalase can led to the accumulation of trehalose content and the enhancement of drought resistance in the transgenic tobaccos.","PeriodicalId":6396,"journal":{"name":"2010 4th International Conference on Bioinformatics and Biomedical Engineering","volume":"12 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2010-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78249928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sand-Dust Aerosol's Impact on Urban Environment in Xinjiang","authors":"W. Gao, Yu-jiang Yuan, Zhihui Liu, Wei Wei","doi":"10.1109/ICBBE.2010.5515151","DOIUrl":"https://doi.org/10.1109/ICBBE.2010.5515151","url":null,"abstract":"Xinjiang is the emission region of sand-dust aerosol as well as the region suffering the severest damage from sand-dust aerosol, whose urban environment especially suffers greater impact from the particles of sand-dust aerosol. The impact on urban environment in Xinjiang is mainly attributed to the sand-dust weather in the multiple sand-dust seasons. The variation trend is in conformity with that of the sand-dust weather. Upon the occurrence of the sand-dust weather, PM10 of the city increases sharply. The sand-dust aerosol turns into the most important pollutant. In the multiple sand-dust seasons, the sand-dust aerosol is the primary pollutant. In the non-heating season, the content percentage of the sand-dust aerosol in the pollutants of Crumbs reaches up to 37% and PM10 during the sand-dust weather period changes obviously, indicating the sand-dust weather has great impact on the atmosphere quality of Xinjiang.","PeriodicalId":6396,"journal":{"name":"2010 4th International Conference on Bioinformatics and Biomedical Engineering","volume":"7 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2010-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78433780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biological Treatment of Ammonium-Rich Wastewater with the Partial Nitrification and Anaerobic Ammonium Oxidation","authors":"Q. Xie, Xiaoxia Li, S. Bai, Yan-hong Li","doi":"10.1109/ICBBE.2010.5517526","DOIUrl":"https://doi.org/10.1109/ICBBE.2010.5517526","url":null,"abstract":"The aim of the investigation was to evaluate the nitrogen removal efficiency by using the combined partial nitrification and anaerobic ammonium oxidation (anammox) process for high-strength ammonium synthetic wastewater. The partial nitrification and anaerobic ammonium oxidation reactors were inoculate with domesticated sludge and operated respectively during the startup period. Subsequently, they were combined for stable operation when the partial nitrification effluent was suitable for being the influent of the anaerobic ammonium oxidation. The startup period was about 40 days. The experimental results of the nitrogen removal performance during the startup and the stable operation periods indicate that over 90% of the inlet nitrogen load to the combined reactors was removed. With the influent concentrations of 360 - 400mg NH4+-N /L, the mean NH4+-N, NO2--N and TN concentrations of the combined process effluent were 2.7mg/L, 0.51mg/L and 55.9mg/L, respectively. And their mean removal efficiencies were 93.7%, 96.2% and 84.6%, correspondingly.","PeriodicalId":6396,"journal":{"name":"2010 4th International Conference on Bioinformatics and Biomedical Engineering","volume":"17 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2010-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78441862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haiying Chen, A. Ding, Junfeng Dou, Lirong Cheng, Fuqiang Fan, Yongchao Du, Xiaona Liu
{"title":"Optimal Conditions for Biodegradation of Indeno (1,2,3-cd) Pyrene in Soil Slurry Reactors","authors":"Haiying Chen, A. Ding, Junfeng Dou, Lirong Cheng, Fuqiang Fan, Yongchao Du, Xiaona Liu","doi":"10.1109/ICBBE.2010.5517395","DOIUrl":"https://doi.org/10.1109/ICBBE.2010.5517395","url":null,"abstract":"Soil slurry reactor technology is one of the effective methods for treating PAHs contaminated soil. In this paper, removal of high molecular weight PAHs, Indeno (1,2,3-cd) pyrene (Inp), was studied in soil slurry reactors with isolated bacteria from PAHs contaminated soil. The factors affecting Inp biodegradation and the optimal conditions were investigated using orthogonal experimental design (L9(34)). The highest degradation efficiency of Inp was 50.71% after the incubation of 12 days. The most significant influencing factors were aeration rate, water to soil ratio and salinity, while pH was less significant. The optimal conditions for Inp degradation in soil slurry reactor was: aeration rate 120L/h, water to soil ratio 2:1, salinity 10%, pH=6.","PeriodicalId":6396,"journal":{"name":"2010 4th International Conference on Bioinformatics and Biomedical Engineering","volume":"67 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2010-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75938561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ping Zhang, T. Ouyang, Shanshan Chen, Ruoshang Li, Limin Lai
{"title":"Fluoride Adsorption onto a Ce-La Binary Hydroxide Adsorbent as Recovery of Ceria Glass Polishing Wastes","authors":"Ping Zhang, T. Ouyang, Shanshan Chen, Ruoshang Li, Limin Lai","doi":"10.1109/ICBBE.2010.5516166","DOIUrl":"https://doi.org/10.1109/ICBBE.2010.5516166","url":null,"abstract":"The adsorption of fluoride onto a Ce−La binary hydroxide adsorbent, herein referred to as CLH adsorbent, was studied under various pH values, adsorbent dose, contact time, and the presence of major anions. In the pH range of 4 to 8, the fluoride adsorption onto CLH reaches a maximum, then decreases with further increasing of pH. A considerably higher adsorption capacity (77.4−89.5 mg g-1) was maintained until the solution pH was up to 8 compared with that of the commercially available activated alumina; indicative of the CLH material should be effective to fluoride removal for major fresh water supplies. The effect of contact time was dependent on the initial fluoride concentration but adsorption equilibriums were reached after 120 min under the initial concentrations of 10 and 25 mg L-1, whereas a residual F- concentration of < 1.5 mg F L-1 was attained that satisfied the guideline value suggested by WHO for fluoride. The adsorptive rate of fluoride on the adsorbent obeys pseudo-second order kinetic models (R2 > 0.99). Adsorption isotherm at pH 7.0 fits best the Langmuir than the Freundlich model of adsorption over the F- concentration range 2−250 mg L-1. A combination of coulombic interaction and the formation of inner-sphere complexes between the activated surface sites and fluoride anions seems to be the adsorption mechanism. Major co-existing anions reduced fluoride adsorption according to their affinity on the CLH surface in the following order: HPO42- > HCO3- ≥ SiO32- > SO42- > Cl-, NO3-.","PeriodicalId":6396,"journal":{"name":"2010 4th International Conference on Bioinformatics and Biomedical Engineering","volume":"79 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2010-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76136990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pesticide Knowledge, Attitudes and Practices in China","authors":"Daqi Xu","doi":"10.1109/ICBBE.2010.5516589","DOIUrl":"https://doi.org/10.1109/ICBBE.2010.5516589","url":null,"abstract":"Pesticides are widely used in China for crop protection. Econometric analysis showed the magnitude of the chronic health effects and health costs to be directly related to pesticide exposure, and accumulative pesticides has led to serious pollution to the environment. This study was conducted on a random sample of 150 rice farming households to collect information on their knowledge, attitude, and behavior related to pesticides in the Anhui province, south of China. Most of farmers we fund did not took incomplete preventive measures. Pesticide information, instruction, and training among farmers should be promoted, and governmental intervention is needed to ensure proper management regarding public health risks and environmental hazards.","PeriodicalId":6396,"journal":{"name":"2010 4th International Conference on Bioinformatics and Biomedical Engineering","volume":"38 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2010-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75036319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}