TMR生命研究 Pub Date : 2021-01-01 DOI: 10.53388/life2021-0111-301

Aliza Ayaz

引用次数: 0

Development of a new field-deployable RT-qPCR workflow for COVID-19 detection 开发新的现场可部署的COVID-19检测RT-qPCR工作流程

TMR生命研究 Pub Date : 2021-01-01 DOI: 10.53388/life2021-0525-110

Raphael Nyaruaba, Bo Zhang, Caroline Muema, E. Muturi, Greater K., Jin Xiong, Bei Li, Z. Shí, Caroline Mwaliko, Junping Yu, Xiaohong Li, Yanan Zhang, H. Wei

{"title":"Development of a new field-deployable RT-qPCR workflow for COVID-19 detection","authors":"Raphael Nyaruaba, Bo Zhang, Caroline Muema, E. Muturi, Greater K., Jin Xiong, Bei Li, Z. Shí, Caroline Mwaliko, Junping Yu, Xiaohong Li, Yanan Zhang, H. Wei","doi":"10.53388/life2021-0525-110","DOIUrl":"https://doi.org/10.53388/life2021-0525-110","url":null,"abstract":"Background: Outbreaks of coronavirus disease 2019 (COVID-19) have been recorded in different countries across the globe. The virus is highly contagious, hence early detection, isolation, and quarantine of infected patients will play an important role in containing the viral spread. Diagnosis in a mobile lab can aid to find infected patients in time. Methods: Here, we develop a field-deployable diagnostic workflow that can reliably detect COVID-19. Instruments used in this workflow can easily fit in a mobile cabin hospital and also be installed in the community. Different steps from sample inactivation to detection were optimized to find the fastest steps and portable instruments in the detection of COVID-19. Each step was compared to that of the normal laboratory diagnosis setup. Results: From the results, our proposed workflow (80 min) was two times faster compared to that of the normal laboratory workflow (183 min) and a maximum of 32 samples could be detected at each run. Additionally, we showed that using 1% Rewocid WK-30 could inactivate the novel coronavirus directly without affecting the overall detection results. Comparison of our workflow using an in-house assay to that of a commercially acquired assay produced highly reliable results. From the 250 hospital samples tested, there was a high concordance 247/250 (98.8%) between the two assays. The in-house assay sensitivity and specificity were 116/116 (100%) and 131/134 (97.8%) compared to that of the commercial assay. Conclusion: Based on these results, we believe that our workflow is fast, reliable, adaptable and most importantly, field-deployable.","PeriodicalId":61869,"journal":{"name":"TMR生命研究","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70813171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Coronary heart disease-related fatigue: risk factors, assessment and treatments 冠心病相关疲劳:危险因素、评估和治疗

TMR生命研究 Pub Date : 2021-01-01 DOI: 10.53388/life2021-0929-334

Yaoyao Hu, Lucun Yu, Jing Zhang, F. Mao, Qing Wu, Lin Liu

引用次数: 0

Pathogenesis of the Chikungunya virus and the host immunity response 基孔肯雅病毒的发病机制及宿主免疫反应

TMR生命研究 Pub Date : 2021-01-01 DOI: 10.53388/life2021-1229-301.

T. Ahmed, S. Abdullah, Rehman Tanvir, Zobayda Nahar, N. Tasnim, Chandrika Saha, R. Noor

引用次数: 2

Amyloid beta peptides, tau protein acetylation and therapeutic strategies for treating Alzheimer’s disease: a review 淀粉样β肽、tau蛋白乙酰化及治疗阿尔茨海默病的策略综述

TMR生命研究 Pub Date : 2021-01-01 DOI: 10.53388/life2021-0121-305

Sanjib Guha, Rajalakshmi Subramaniyam