细胞与分子免疫学杂志最新文献

{"title":"[Advances in tumor-associated macrophage research driven by single-cell sequencing and spatial omics technologies].","authors":"Qianqian An, Xiaojian Wang, Hongyan Qin, Yiyang Hu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Malignant tumors pose a substantial threat to human health and express a pressing public health concern. Tumor-associated macrophage (TAM) exhibit remarkable heterogeneity and play pivotal roles within the tumor micro-environment (TME). However, the conventional M1/M2 polarization framework fails to capture the full spectrum of their functional complexity. Recent advances in single-cell RNA sequencing (scRNA-seq) and spatial omics technologies have enabled the identification of multiple transcriptionally distinct and functionally specialized TAM subsets across various solid tumors, including glioma, liver cancer, and breast cancer. The discovery and characterization of these novel TAM populations have provided critical insights into the mechanisms underlying tumor progression and therapeutic resistance, thereby uncovering potential therapeutic targets and informing the development of precision immunotherapeutic strategies tailored to specific TAM subpopulations.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 4","pages":"373-382"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147790363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Development of a cell-based imaging assay for evaluating neutralizing antibodies against western equine encephalitis virus].","authors":"Yun Cui, Xinyu Cai, Youdi Zhang, Yuying Ning, Luomiaoyuan Zhang, Ruodong Yuan, Yangchao Dong, Songna Yin, Yingfeng Lei","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Objective To establish a rapid, high-throughput, and visualisation method for evaluating neutralizing antibodies against western equine encephalitis virus (WEEV) based on cell imaging technology. Methods Recombinant virus rVSV-WEEV-GFP, previously constructed by our research group, was propagated in baby hamster kidney cells (BHK-21) and subsequently titrated. The infection efficiency of rVSV-WEEV-GFP was assessed in three cell lines-BHK-21, African green monkey kidney cells (Vero), and mouse neuroblastoma cells (Neuro2a) via fluorescence intensity analysis based on a high-throughput microplate imaging system (Cytation5). A series of experiments were performed to optimize the key conditions for a neutralizing antibody assay including the mean fluorescence intensity in rVSV-WEEV-GFP-infected cells across different time points, MOIs, and cell seeding densities. Accordingly, a robust method based on cellular imaging was established. The consistency between the two methods was further evaluated by comparing the half-maximal inhibitory concentration (IC₅₀) of a broadly neutralizing antibody measured using both this novel imaging-based assay and the conventional plaque reduction neutralization test (PRNT). Finally, the neutralizing activity of previously prepared encephalitic alphavirus antibodies was analysed using the novel assay. Results Vero cells were regarded as the most efficient cell line for rVSV-WEEV-GFP infection. The optimal detection time and multiplicity of infection (MOI) were identified as 36 hours and 0.0625, respectively. And, the seeding optimal cell density was 10 000 cells per well. The cell imaging-based neutralization assay demonstrated high consistency with the conventional plaque reduction neutralization test (PRNT). Among six antibodies tested, three exhibited potent neutralizing activity against WEEV. Conclusion The cell imaging-based neutralization assay established in this study provides a reliable foundation for the screening of antibodies with high neutralizing activity against WEEV.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 4","pages":"332-338"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147790376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Latest advances in neutrophil extracellular trap-mediated tissue damage, organ crosstalk, and therapeutic targeting following acute kidney injury].","authors":"Xinran Xie, Manling Zhang, Yong Jin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Acute kidney injury (AKI) is a complex clinical syndrome characterized by a rapid decline in renal function, triggered by diverse factors. The precise pathogenesis of AKI remains incompletely understood. In recent years, neutrophil extracellular traps (NETs), a unique form of innate immune defense, have garnered significant attention within the AKI research field. NETs are extracellular fibers released by neutrophils upon external stimulation and their components include DNA, histones, and various proteases. During the progression of AKI, NETs formation not only exacerbates renal inflammation and tissue damage, but also mediates interactions between the kidneys and distant organs. The in-depth investigation into the mechanisms of NETs release and action in AKI holds promise for revealing novel therapeutic targets for its prevention and treatment. This review aims to summarize the mechanisms of NETs formation and release, discuss their role in AKI pathogenesis, explore the therapeutic potential of targeting NETs for AKI, and elaborate on the NETs-mediated cross-talk between organs in AKI, thereby offering new perspectives for related research of AKI.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 3","pages":"259-266"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147610638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research advances on polyunsaturated fatty acids in pediatric asthma].","authors":"Han Zhou, Min Wang, Yue Qin, Qianying Liu, Manqi Xiong, Huajie Wu, Wei Zhang, Xin Sun","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pediatric asthma is a chronic inflammatory disorder characterized by reversible airway obstruction. Nevertheless, severe cases are frequently complicated by acute exacerbations and carry an elevated risk of progression to irreversible airflow limitation. Given that immune dysregulation underpins this pathogenic process, the majority of affected children exhibit a Type 2 (T2) inflammatory phenotype. This phenotype is defined by eosinophil, mast cell, and basophil infiltration-processes predominantly driven by T-helper 2 (Th2) cells and group 2 innate lymphoid cells (ILC2s). As essential dietary nutrients, polyunsaturated fatty acids (PUFAs) exert profound regulatory effects on these pathophysiological pathways, modulating immune-inflammatory responses and supporting gut microbial homeostasis. Accordingly, this review provides a systematic summary of the biological properties of omega-3 and omega-6 PUFAs, with particular emphasis on their functional roles and underlying molecular mechanisms in pediatric asthma, aiming to provide evidence-based strategies for early prevention and precision nutritional intervention.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 3","pages":"267-273"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147610690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Foxo1 mediates immune damage in autoimmune thyroiditis by regulating the differentiation and function of Th17 cells].","authors":"Ling Gan, Chengyan He, Jue Wang, Haibo Xue","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Objective To explore the roles and mechanisms of forkhead box protein O1(Foxo1) in the occurrence and immune injury of experimental autoimmune thyroiditis (EAT). Methods In vivo experiments, thirty female C57BL/6 mice were randomized into three groups: NC (normal control) group, EAT (pTg-induced model via multi-point subcutaneous injection) group, and EAT+AS (EAT mice administered AS1842856 intraperitoneally) group. At the end of the 8th week, the thyroid, serum and spleen tissues were collected. In vitro experiments, 0 nmol/L, 30 nmol/L and 60 nmol/L AS1842856 were used to interfere with the cytokine polarized spleen mononuclear cells(SMCs) for 24 hours, and the cells were collected for follow-up experiments. HE staining was used to evaluate the degree of inflammation in the thyroid tissue of mice; ELISA was used to detect the thyroglobulin antibody(TgAb) and interleukin 17A(IL-17A) concentrations in the serum; flow cytometry was used to detect the proportion of T helper 17(Th17) cells in SMCs; Western blot was used to detect the protein expression levels of Foxo1, phosphorylation Foxo1(p-Foxo1), retinoic acid-related orphan receptor γt(RORγt) and IL-17A; qPCR was used to detect the mRNA levels of RORγt and IL-17A. Results The thyroid showed varying degrees of follicular destruction and lymphocyte infiltration in the EAT group; the levels of serum TgAb and IL-17A were significantly increased; the total protein expression of Foxo1 had no significant change, while the p-Foxo1 protein was obviously increased; The proportion of Th17 cells in SMCs, spleen index, the protein and mRNA levels of RORγt and IL-17A were all increased remarkably, and all were positively correlated with the phosphorylation level of Foxo1. The degree of thyroid destruction and lymphocyte infiltration in the EAT+AS group was aggravated; the serum TgAb and IL-17A concentrations, the proportion of Th17 cells in SMCs, spleen index, and the protein and mRNA levels of RORγt and IL-17A were all significantly increased. Additionally, the proportion of Th17 cells and the expressions of RORγt and IL-17A increased in a concentration-dependent manner with the increase of the concentration of AS1842856 in vitro. Conclusion The enhanced phosphorylation-mediated inactivation of Foxo1 weakens its inhibitory effect on Th17 cells in EAT mice, and then promotes the differentiation of Th17 cells and the secretion of IL-17A, ultimately participating in the onset of EAT and the thyroid autoimmune damage.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 3","pages":"202-209"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147610615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Recombinant expression of rift valley fever virus nucleocapsid protein and generation of rabbit polyclonal antibodies].","authors":"Xuemin Pei, Dongshen Jiang, Xiaojing Yang, Qiqi Yang, Hui Zhang, Jiawei Pei, Jiahong Zhu, Yingfeng Lei, Wei Ye, Fanglin Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Objective Prokaryotic expression and purification of the Rift Valley fever virus (RVFV) nucleocapsid protein (NP) were performed to establish a basis for serum NP antibody detection and to produce specific polyclonal antibodies against NP. Methods The constructed pET28a-ZH501-NP prokaryotic expression plasmid was transformed into BL21(DE3) competent cells, and the expression of the recombinant protein NP was induced by isopropyl-β-D-thiogalactoside (IPTG). The recombinant protein NP was purified using HisTrap^TM HP and mixed with Freund's complete and incomplete adjuvants to immunize New Zealand white rabbits. NP polyclonal antibodies were prepared, and their specificity was identified by indirect immunofluorescence (IFA) and Western Blotting. Results The prokaryotic expression plasmid was successfully constructed, and expression was successfully induced in BL21(DE3) competent cells. The recombinant protein NP was expressed in the form of inclusion bodies. Western Blotting and IFA assays indicated that the polyclonal antibody could specifically recognize RVFV NP. Conclusion The pure and effective expression of RVFV NP protein was achieved. The experimental conditions for the indirect ELISA using it as the coating antigen were explored. Simultaneously, specific polyclonal antibodies against RVFV NP were prepared, providing technical support for the establishment of a reliable rapid diagnostic technology for RVF and the research and evaluation of new RVFV vaccines.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 3","pages":"237-244"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147610628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The molecular mechanism of metformin-adipose mesenchymal stem cell derived exosome complex in regulating macrophage polarization to ameliorate liver ischemia-reperfusion injury].","authors":"Aiwen Jiang, Jiekun Pu, Xueping Zhao, Yi Zhang, Cong Niu, Yong Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Objective This study aimed to investigate the protective effect of a metformin (Met)-adipose-derived mesenchymal stem cell exosome (ADSC-Exo) complex (Met-Exo) against hepatic ischemia-reperfusion injury (IRI) and to determine whether this protection is mediated through the silent information regulator 1(SIRT1)-tissue inhibitor of metalloproteinase 3(TIMP3) axis. Methods Met-Exo was constructed and characterized by nanoparticle tracking analysis, Western blotting, and transmission electron microscopy (TEM). An in vitro model of hepatic IRI was established in mouse hepatocyte AML12 cells subjected to oxygen-glucose deprivation/re-oxygenation (OGD/R). Cell viability, apoptosis, and hepatocyte function markers (AST and ALT) were measured to compare the protective effects of Met-Exo, ADSC-Exo, and Met alone. A Transwell^TM co-culture system was used to evaluate how macrophage polarization influences OGD/R-induced AML12 injury. AML12 cells were assigned to the following groups: Ctrl, OGD/R, Met, ADSC-Exo, Met-Exo, and macrophage-polarization groups (M0, M1, M1+Met-Exo, M1+pcDNA3.1, M1+pcDNA3.1-SIRT1, M1+pcDNA3.1-TIMP3). SIRT1 and TIMP3 protein levels in AML12 cells were determined by Western blot. Macrophage-polarization markers-inducible nitric-oxide synthase (iNOS) and arginase 1 (Arg1) were also quantified. In parallel, AML12 cells' viability, apoptosis, and hepatocellular function indices (AST& ALT) were assessed. A murine hepatic IRI model was constructed, and Met-Exo was administered to assess therapeutic efficacy in vivo. Results Met-Exo was successfully prepared. In vitro, Met-Exo attenuated OGD/R-induced hepatocyte injury, increased viability, reduced apoptosis, and lowered AST and ALT release. Met-Exo pre-treatment suppressed expression of the M1 marker iNOS and enhanced expression the M2 marker Arg1, thereby alleviating OGD/R damage in AML12 cells. OGD/R markedly decreased SIRT1 and TIMP3 expression in AML12 cells, whereas Met-Exo pre-treatment significantly up-regulated SIRT1 and consequently TIMP3 expression. In macrophage polarization experiments, M1 macrophages exacerbated AML12 injury, shown as decreased protein expression of SIRT1 and TIMP3, increased iNOS levels, reduced Arg1 levels, along with diminished AML12 cell viability, increased apoptosis, and elevated AST/ALT. Over-expression of either SIRT1 or TIMP3 reversed these detrimental effects, skewing macrophages toward the M2 phenotype and mitigating AML12 injury. In vivo, Met-Exo markedly ameliorated hepatic IRI in mice. Conclusion Met-Exo protects against hepatic IRI by activating the SIRT1-TIMP3 axis, thereby driving macrophage polarization toward the anti-inflammatory M2 phenotype, attenuating inflammation and apoptosis in hepatocytes subjected to OGD/R.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 3","pages":"210-220"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147610619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Establishment of an integrin β3-overexpressing cell line and its influence on Hantaan virus infection and replication].","authors":"Xing Shen, Shiyuan Hou, Ziyu Liu, Mengting Zhu, Shuai Zhao, Rongrong Liu, Xing'an Wu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Objective To establish a stable cell line overexpressing integrin β3(ITGB3), aiming to overcome the limited replication efficiency and low viral yields of Hantaan virus (HTNV) in conventional cell culture systems. Methods A lentiviral vector encoding ITGB3 was constructed and packaged into viral particles. Vero E6 and A549 cells were transduced and selected with puromycin to generate stable lines. ITGB3 expression was verified by quantitative RT-qPCR, Western blot, and immunofluorescence staining, and cell viability was evaluated using the CCK-8 assay. Following HTNV infection, viral replication was assessed by measuring viral RNA copies, nucleoprotein (NP) expression, and virus titers. Results ITGB3 was stably and highly expressed in Vero E6 and A549 cells, with no significant impact on cell proliferation. After HTNV infection, the levels of viral RNA replication and the expression of NP protein were both significantly upregulated in the ITGB3-overexpressing group, and the virus titers were markedly higher than those in the control group. Conclusion The established ITGB3 overexpressing cell line promotes HTNV replication and propagation, providing a reliable and efficient model for investigating HTNV pathogenesis and facilitating the development of antiviral drugs.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 3","pages":"193-201"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147610544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The evolution and impacts of post-tramatic cell death modalities].","authors":"Xingsheng Wang, Qishun Qin, Shihong Xu, Jiang Wu, Kai Li, Chao Lu, Pei Peng, Yuchen Qin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Trauma-induced cell death modalities, which serve as the initiating events of systemic inflammation and multi-organ dysfunction, have emerged as a pivotal focus in trauma pathology research. After trauma, the activation of distinct programmed cell death pathways exhibits a phased progression and intricate crosstalk, involving multi-tiered regulatory mechanisms such as mitochondrial pathway activation, inflammasome assembly, lipid peroxidation cascades, and dysregulated metal ion homeostasis. Notably, apoptosis, pyroptosis, necroptosis, and ferroptosis successively dominate inflammatory and metabolic dysregulation over time, while PANoptosis, as an integrated cell death program, demonstrates cross-pathway signal convergence. Emerging cell death paradigms further elucidate the complex interplay among mitochondrial stress, metal homeostasis, metabolic reprogramming, and trauma pathology. This review synthesizes the dual roles and therapeutic potential of these cell death modalities in tissue repair, aiming to provide a theoretical framework and intervention targets for the precise modulation of post-traumatic pathological responses.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 3","pages":"274-281"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147610624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Exploring the intervention mechanism of modified Danggui buxue decoction on neuroinflammation in CIPN model mice based on transcriptomics and network pharmacology].","authors":"Jiaqiong Zhang, Yifei Han, Chao Song, Yanwen Chen, Yanjun Wang, Chenxia Lu, Haixia Ming","doi":"","DOIUrl":"","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Objective To investigate the intervention targets of modified Danggui buxue decoction on neuroinflammation in a chemotherapy-induced peripheral neuropathy(CIPN) model mice. Methods Sixty SPF-grade C57BL/6J mice were used. Except for the blank control group, the remaining 48 mice underwent ectopic transplantation of gastric cancer cells into the axilla and were randomly divided into four groups (n=12 per group): the gastric cancer group, the CIPN model group, the modified Danggui buxue decoction group, and Duloxetine group. Except for the gastric cancer group, mice in the other groups received intraperitoneal injections of oxaliplatin to establish a model of CIPN following platinum-based chemotherapy for gastric cancer. The drug intervention groups were administered their respective doses of medicinal solution by gavage, while the remaining groups received an equal volume of normal saline by gavage, once daily. After 28 days, performing behavioral experiments (mechanical pain threshold test and cold stimulation test), the dorsal root ganglia of the mice were collected for analysis of relevant indicators using transcriptomics, TEM, WB, and RT-PCR. Results Behavioral test results showed: After 28 days of administration, compared with the blank control group, the CIPN model group exhibited a downward trend in mechanical pain threshold and an upward trend in the frequency of paw withdrawal in response to cold stimulation. Compared with the CIPN model group, the gastric cancer group, modified Danggui buxue decoction group, and the Duloxetine group showed an upward trend in mechanical pain threshold and a downward trend in the frequency of paw withdrawal in response to cold stimulation. Transcriptomics results indicated that modified Danggui buxue decoctioned its effects by inhibiting NF-κB, ATM, and CCL21. Network pharmacology analysis suggested that modified Danggui buxue decoction might intervene in the occurrence of CIPN by suppressing IL-1β and IL-6 through TNF-α inhibition. TEM results revealed: Except for the blank control group, abnormal myelinated nerve fibers were observed in the dorsal root ganglia of the other four groups. Compared with the blank control group, the CIPN model group exhibited demyelination, dissolution of myelin sheaths, loosely arranged myelin lamellae, disrupted and dissolved mitochondrial cristae, and vacuolation. Compared with the CIPN model group, the modified Danggui buxue decoction group and the Duloxetine group showed alleviation of the aforementioned damage. WB and RT-PCR results showed: Compared with the blank control group, the protein and gene expression levels of ATM, NF-κB, CCL21, TNF-α, IL-1β, and IL-6 in the dorsal root ganglia of the CIPN model group were significantly increased. Compared with the CIPN model group, the protein and gene expression levels of ATM, NF-κB, CCL21, TNF-α, IL-1β, and IL-6 were significantly decreased in the gastric cancer group, modified Danggui buxue decoction group, and the D","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 3","pages":"221-229"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147610565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}