生物物理学报:英文版最新文献_第7页

Reduced graphene oxide membrane as supporting film for high-resolution cryo-EM. 还原氧化石墨烯膜作为高分辨率低温电镜的支撑膜。

生物物理学报:英文版 Pub Date : 2021-06-30 DOI: 10.52601/bpr.2021.210007

Nan Liu, Liming Zheng, Jie Xu, Jia Wang, Cuixia Hu, Jun Lan, Xing Zhang, Jincan Zhang, Kui Xu, Hang Cheng, Zi Yang, Xin Gao, Xinquan Wang, Hailin Peng, Yanan Chen, Hong-Wei Wang

{"title":"Reduced graphene oxide membrane as supporting film for high-resolution cryo-EM.","authors":"Nan Liu, Liming Zheng, Jie Xu, Jia Wang, Cuixia Hu, Jun Lan, Xing Zhang, Jincan Zhang, Kui Xu, Hang Cheng, Zi Yang, Xin Gao, Xinquan Wang, Hailin Peng, Yanan Chen, Hong-Wei Wang","doi":"10.52601/bpr.2021.210007","DOIUrl":"https://doi.org/10.52601/bpr.2021.210007","url":null,"abstract":"Although single-particle cryogenic electron microscopy (cryo-EM) has been applied extensively for elucidating many crucial biological mechanisms at the molecular level, this technique still faces critical challenges, the major one of which is to prepare the high-quality cryo-EM specimen. Aiming to achieve a more reproducible and efficient cryo-EM specimen preparation, novel supporting films including graphene-based two-dimensional materials have been explored in recent years. Here we report a robust and simple method to fabricate EM grids coated with single- or few-layer reduced graphene oxide (RGO) membrane in large batch for high-resolution cryo-EM structural determination. The RGO membrane has decreased interlayer space and enhanced electrical conductivity in comparison to regular graphene oxide (GO) membrane. Moreover, we found that the RGO supporting film exhibited nice particle-absorption ability, thus avoiding the air-water interface problem. More importantly, we found that the RGO supporting film is particularly useful in cryo-EM reconstruction of sub-100-kDa biomolecules at near-atomic resolution, as exemplified by the study of RBD-ACE2 complex and other small protein molecules. We envision that the RGO membranes can be used as a robust graphene-based supporting film in cryo-EM specimen preparation.","PeriodicalId":59621,"journal":{"name":"生物物理学报:英文版","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10244798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9596783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Potential of adipose derived stem cell preparations in neurological repair and regeneration. 脂肪源性干细胞在神经修复和再生中的潜力。

生物物理学报:英文版 Pub Date : 2021-04-30 DOI: 10.52601/bpr.2021.200025

Laura Combes, Xenia Sawkulycz, Wen-Hui Fang, Baoqiang Guo, Mark Slevin

引用次数: 0

Live-cell fluorescence imaging of ciliary dynamics. 纤毛动态的活细胞荧光成像。

生物物理学报:英文版 Pub Date : 2021-04-30 DOI: 10.52601/bpr.2021.210005

Quanlong Lü, Chuanmao Zhang, Christopher J Westlake

引用次数: 2

Genetic variation and function: revealing potential factors associated with microbial phenotypes. 遗传变异和功能:揭示与微生物表型相关的潜在因素。

生物物理学报:英文版 Pub Date : 2021-04-30 DOI: 10.52601/bpr.2021.200040

Xiaolin Liu, Yue Ma, Jun Wang

{"title":"Genetic variation and function: revealing potential factors associated with microbial phenotypes.","authors":"Xiaolin Liu, Yue Ma, Jun Wang","doi":"10.52601/bpr.2021.200040","DOIUrl":"https://doi.org/10.52601/bpr.2021.200040","url":null,"abstract":"Innovations in sequencing technology have generated voluminous microbial and host genomic data, making it possible to detect these genetic variations and analyze the function influenced by them. Recently, many studies have linked such genetic variations to phenotypes through association or comparative analysis, which have further advanced our understanding of multiple microbial functions. In this review, we summarized the application of association analysis in microbes like Mycobacterium tuberculosis, focusing on screening of microbial genetic variants potentially associated with phenotypes such as drug resistance, pathogenesis and novel drug targets etc.; reviewed the application of additional comparative genomic or transcriptomic methods to identify genetic factors associated with functions in microbes; expanded the scope of our study to focus on host genetic factors associated with certain microbes or microbiome and summarized the recent host genetic variations associated with microbial phenotypes, including susceptibility and load after infection of HIV, presence/absence of different taxa, and quantitative traits of microbiome, and lastly, discussed the challenges that may be encountered and the apparent or potential viable solutions. Gene-function analysis of microbe and microbiome is still in its infancy, and in order to unleash its full potential, it is necessary to understand its history, current status, and the challenges hindering its development.","PeriodicalId":59621,"journal":{"name":"生物物理学报:英文版","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9653026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Optimizing weighting functions for cryo-electron microscopy. 优化冷冻电子显微镜的权重函数。

生物物理学报:英文版 Pub Date : 2021-04-30 DOI: 10.52601/bpr.2021.210001

Jing Cheng, Xinzheng Zhang

引用次数: 2

Mammalian mitochondrial iron-sulfur cluster biogenesis and transfer and related human diseases. 哺乳动物线粒体铁硫簇的生物发生和转移及相关的人类疾病。

生物物理学报:英文版 Pub Date : 2021-04-30 DOI: 10.52601/bpr.2021.200038

Wenxin Zhang, Li Xu, Hongting Zhao, Kuanyu Li

{"title":"Mammalian mitochondrial iron-sulfur cluster biogenesis and transfer and related human diseases.","authors":"Wenxin Zhang, Li Xu, Hongting Zhao, Kuanyu Li","doi":"10.52601/bpr.2021.200038","DOIUrl":"https://doi.org/10.52601/bpr.2021.200038","url":null,"abstract":"As a cofactor, iron-sulfur (Fe-S) cluster binds to proteins or enzymes that play important roles in various important biological processes, including DNA synthesis and repair, mitochondrial function, gene transcription and translation. In mammals, the core components involved in Fe-S cluster biosynthesis are considered to include the scaffold protein ISCU, cysteine desulfurase NFS1 and its accessory proteins ISD11 and ACP, and frataxin (FXN). Proteins involved in Fe-S cluster transfer have been found to include HSC20/HSPA9, as chaperone system, and Fe-S cluster carriers. The biosynthesis and transfer of Fe-S clusters to Fe-S recipients require fine-tune regulation. Recently, significant progress has been made in the structure and mechanism of mitochondrial Fe-S biosynthesis and transfer. Based on, especially, the development of DNA sequencing technology, bioinformatics, and gene editing technology, diseases caused by mutations of Fe-S cluster-related genes have been revealed in recent years, promoting the rapid development in the field of Fe-S and human health. This review focuses on the function of genes involved in Fe-S cluster biosynthesis and transfer and on the diseases caused by the mutations of the related genes. Finally, some questions we are facing are raised, new hypotheses presented, and the perspectives discussed.","PeriodicalId":59621,"journal":{"name":"生物物理学报:英文版","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9653022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Anti-oxidative effect of zinc in human umbilical cord mesenchymal stem cells. 锌对人脐带间充质干细胞的抗氧化作用。

生物物理学报:英文版 Pub Date : 2021-04-30 DOI: 10.52601/bpr.2021.200046

Xiaodan Lu, Yifan Lin, Xiuying Lin, Qiang Zhang, Zihang Wang, Xuguang Mi, Ruobing Wang, Xiaofang Zhang, Xu Luan, Yan Liu, Bing Li, Yan Tan, Yanqiu Fang

{"title":"Anti-oxidative effect of zinc in human umbilical cord mesenchymal stem cells.","authors":"Xiaodan Lu, Yifan Lin, Xiuying Lin, Qiang Zhang, Zihang Wang, Xuguang Mi, Ruobing Wang, Xiaofang Zhang, Xu Luan, Yan Liu, Bing Li, Yan Tan, Yanqiu Fang","doi":"10.52601/bpr.2021.200046","DOIUrl":"https://doi.org/10.52601/bpr.2021.200046","url":null,"abstract":"Human umbilical cord mesenchymal stem cells (HUC-MSCs) are pluripotent and functional in many biological processes, by which releasing secretary factors to promote the self-repairing of damaged tissue or developing into functional cell at local organ. However, there is a high risk that oxidative stress would reduce the pluripotency and factor-secretion during the preparation and transplantation. Therefore, reducing oxidative stress is expected to improve the efficacy of HUC-MSCs therapy. Zinc (Zn) is an essential trace element which involves in the resistance of oxidative stress. To investigate Zn-regulated signaling pathways, we have profiled the gene expression at transcriptome level in primary HUC-MSCs treated with zinc sulfate, followed with GO and KEGG gene enrichment analysis. Zn treatment improved signal pathways for mineral absorption, cell growth, and cell death. Zn deficiency was mimicked by TPEN administration, which suppressed cell proliferation and reduced the expression of HUC-MSCs surface stem cell markers CD73, CD90 and CD105 by flow cytometry. Nuclear factor erythrocyte 2 related factor 2 (Nrf2) plays an important role in antioxidant biological processes. In vitro treatment of Zn significantly increased Nrf2 and Sirt3 expression at gene level and protein level respectively. Zn supplementation inhibited TPEN-induced failure of cell survival and reversed the reduction of Nrf2 and Sirt3 expression, which further reduced the production of ROS. Zn successfully presented its anti-oxidation effect by activating Nrf2/Sirt3 signaling pathway in HUC-MSCs. Zn supplementation may improve the efficacy of HUC-MSCs therapy with reduced oxidative stress.","PeriodicalId":59621,"journal":{"name":"生物物理学报:英文版","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235909/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9600573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

AP-3 adaptor complex-mediated vesicle trafficking. AP-3接头复合物介导的囊泡运输。

生物物理学报:英文版 Pub Date : 2021-04-30 DOI: 10.52601/bpr.2021.200051

Zhuo Ma, Md Nur Islam, Tao Xu, Eli Song

{"title":"AP-3 adaptor complex-mediated vesicle trafficking.","authors":"Zhuo Ma, Md Nur Islam, Tao Xu, Eli Song","doi":"10.52601/bpr.2021.200051","DOIUrl":"https://doi.org/10.52601/bpr.2021.200051","url":null,"abstract":"The transport of cargo proteins to specific subcellular destinations is crucial for the different secretory and endocytic traffic pathways. One of the most important steps in maintaining the accuracy of this process is the recruitment of adaptor protein (AP) complexes to the membrane for recognizing and packaging cargo proteins into nascent vesicles. Adaptor protein complex 3 (AP-3) is a heterotetrametric complex implicated in the trafficking of cargo proteins from the trans-Golgi network (TGN) and/or endosomes to lysosomes or lysosome-related organelles (LROs). This complex is also involved in the biogenesis of synaptic vesicles (SVs) in neurons and of dense core vesicles (DCVs) in endocrine cells as well as in the recycling of receptors in immune cells and the regulation of planar cell polarity (PCP) proteins. Functional defects in AP-3 cause multiple abnormalities in cellular vesicle trafficking and related organelle function, leading to various disorders, such as Hermansky-Pudlak syndrome (HPS). However, the molecular mechanism underlying AP-3 has not been fully elucidated, and further investigations are needed to understand AP-3-mediated trafficking, its associated molecules and its related roles in inherited diseases. Here, we review the current understanding of AP-3 in cellular vesicle trafficking, especially focusing on mammalian systems.","PeriodicalId":59621,"journal":{"name":"生物物理学报:英文版","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9603184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduced graphene oxide membrane as supporting film for high-resolution cryo-EM 还原氧化石墨烯膜作为高分辨率低温电镜的支撑膜

生物物理学报:英文版 Pub Date : 2021-04-15 DOI: 10.1101/2021.04.15.439953

Nan Liu, Liming Zheng, Jie Xu, Jia Wang, Cuixia Hu, Jun Lan, Xing Zhang, Jincan Zhang, Kui Xu, Hang Cheng, Zi Yang, Xingyong Gao, Xinquan Wang, Hailin Peng, Yanan Chen, Hong-Wei Wang

{"title":"Reduced graphene oxide membrane as supporting film for high-resolution cryo-EM","authors":"Nan Liu, Liming Zheng, Jie Xu, Jia Wang, Cuixia Hu, Jun Lan, Xing Zhang, Jincan Zhang, Kui Xu, Hang Cheng, Zi Yang, Xingyong Gao, Xinquan Wang, Hailin Peng, Yanan Chen, Hong-Wei Wang","doi":"10.1101/2021.04.15.439953","DOIUrl":"https://doi.org/10.1101/2021.04.15.439953","url":null,"abstract":"Although single-particle cryogenic electron microscopy (cryo-EM) has been applied extensively for elucidating many crucial biological mechanisms at the molecular level, this technique still faces critical challenges, the major one of which is to prepare the high-quality cryo-EM specimen. Aiming to achieve a more reproducible and efficient cryo-EM specimen preparation, novel supporting films including graphene-based two-dimensional materials have been explored in recent years. Here we report a robust and simple method to fabricate EM grids coated with single- or few-layer reduced graphene oxide (RGO) membrane in large batch for high-resolution cryo-EM structural determination. The RGO membrane has decreased interlayer space and enhanced electrical conductivity in comparison to regular graphene oxide (GO) membrane. Moreover, we found that the RGO supporting film exhibited nice particle-absorption ability, thus avoiding the air-water interface problem. More importantly, we found that the RGO supporting film is particularly useful in cryo-EM reconstruction of sub-100 kDa biomolecules at near-atomic resolution, as exemplified by the study of RBD-ACE2 complex and other small protein molecules. We envision that the RGO membranes can be used as a robust graphene-based supporting film in cryo-EM specimen preparation.","PeriodicalId":59621,"journal":{"name":"生物物理学报:英文版","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"62326634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Nanozymes with bioorthogonal reaction for intelligence nanorobots. 智能纳米机器人的生物正交反应纳米酶。

生物物理学报:英文版 Pub Date : 2021-02-28 DOI: 10.52601/bpr.2021.200044

Si Sun, Xinzhu Chen, Jing Chen, Junying Wang, Xiao-Dong Zhang

{"title":"Nanozymes with bioorthogonal reaction for intelligence nanorobots.","authors":"Si Sun, Xinzhu Chen, Jing Chen, Junying Wang, Xiao-Dong Zhang","doi":"10.52601/bpr.2021.200044","DOIUrl":"https://doi.org/10.52601/bpr.2021.200044","url":null,"abstract":"Bioorthogonal reactions have attained great interest and achievements in various fields since its first appearance in 2003. Compared to traditional chemical reactions, bioorthogonal chemical reactions mediated by transition metals catalysts can occur under physiological conditions in the living system without interfering with or damaging other biochemical events happening simultaneously. The idea of using nanomachines to perform precise and specific tasks in living systems is regarded as the frontier in nanomedicine. Bioorthogonal chemical reactions and nanozymes have provided new potential and strategies for nanomachines used in biomedical fields such as drug release, imaging, and bioengineering. Nanomachines, also called as intelligence nanorobots, based on nanozymes with bioorthogonal reactions show better biocompatibility and water solubility in living systems and perform controlled and adjustable stimuli-triggered response regarding to different physiological environments. In this review, we review the definition and development of bioorthogonal chemical reactions and describe the basic principle of bioorthogonal nanozymes fabrication. We also review several controlled and adjustable stimuli-triggered intelligence nanorobots and their potential in therapeutic and engineered applications. Furthermore, we summarize the challenges in the use of intelligence nanorobots based on nanozymes with bioorthogonal chemical reactions and propose promising vision in smart nanodevices along this appealing avenue of research.","PeriodicalId":59621,"journal":{"name":"生物物理学报:英文版","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10240535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9603178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0