Pharmaceutical Sciences and Research最新文献_第3页

Discovery of SARS-CoV-2 RNA-dependent-RNA-polymerase (RdRp) Inhibitor from Sambiloto (Andrographis paniculata) Based on Molecular Docking and ADMET Prediction Approach 基于分子对接和ADMET预测方法从穿心莲中发现严重急性呼吸系统综合征冠状病毒2型RNA依赖性RNA聚合酶（RdRp）抑制剂

Pharmaceutical Sciences and Research Pub Date : 2022-08-01 DOI: 10.7454/psr.v9i2.1236

R. R. Pratama, Alfisyahriatunnida Meily, Andika

{"title":"Discovery of SARS-CoV-2 RNA-dependent-RNA-polymerase (RdRp) Inhibitor from Sambiloto (Andrographis paniculata) Based on Molecular Docking and ADMET Prediction Approach","authors":"R. R. Pratama, Alfisyahriatunnida Meily, Andika","doi":"10.7454/psr.v9i2.1236","DOIUrl":"https://doi.org/10.7454/psr.v9i2.1236","url":null,"abstract":"The rapid spread of the coronavirus disease 2019 (COVID-19) has led to the development of therapeutic inhibitor drug of SARS-CoV-2, which can inhibit the viral enzyme RNA-dependent-RNA-polymerase (RdRp), thereby preventing the replication, transcription, and synthesis of RNA virus in the host cells. Previous in-vitro studies revealed that Andrographis paniculata has the potential to inhibit the virus. Therefore, this study aims to isolate the specific compounds of Andrographis paniculata , which play a role in inhibiting SARS-CoV-2 RdRp using molecular docking. A total of 19 compounds were identified in previous literature studies, while remdesivir and favipiravir were used as the positive control. All compounds and proteins were applied to minimize and optimize energy. Furthermore, the docking method was carried out using Autodock 4.2.6 software with a specific grid box containing the active site of RdRp (ID: 6M71), and the Lamarckian Genetic Algorithm was used to determine the conformation. The best docking was screened on ADMET prediction and the binding energy was evaluated. There are 18 compounds of Andrographis paniculata including the top three, namely andrographolactone (∆G = -8.86 kcal/mol), andrographolide (∆G = -7.74 kcal/mol), and andrographidine-A (∆G = -7.68 kcal/ mol), which showed the strongest binding affinity to the SARS-CoV-2 RdRp protein compared to other compounds and the positive control remdesivir (∆G = -5.73 kcal/mol) and favipiravir (∆G = -5.20 kcal/ mol). Furthermore, active amino acids play a role in this interaction by forming strong hydrogen bonds, such as TYR 619, LYS 621, ASP 760, and ASP 623. Andrographolactone has the highest potential as SARS-CoV-2 RdRp inhibitor, hence, it can be used as a novel therapeutic candidate.","PeriodicalId":55754,"journal":{"name":"Pharmaceutical Sciences and Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45616363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Challenge and Development Strategy for Colon-Targeted Drug Delivery System 结肠靶向给药系统的挑战与发展策略

Pharmaceutical Sciences and Research Pub Date : 2022-05-18 DOI: 10.7454/psr.v9i1.1251

R. Iswandana, K. Putri, Fathia Amalia Putri, Maxius Gunawan, Sekar, Arum Larasati

引用次数: 3

Mitragyna speciosa as a Potential Substitute Therapy in Opioid Dependence: A Case Report 米特拉特纳作为阿片类药物依赖的潜在替代疗法:1例报告

Pharmaceutical Sciences and Research Pub Date : 2022-05-18 DOI: 10.7454/psr.v9i1.1215

K. Khalid, J. Wong, R. Jamaluddin

引用次数: 1

CRISPR/Cas9 Genome Editing: Future Treatment of Duane Retraction Syndrome CRISPR/Cas9基因组编辑:Duane回缩综合征的未来治疗

Pharmaceutical Sciences and Research Pub Date : 2022-05-18 DOI: 10.7454/psr.v9i1.1231

B. W. Wardhani

引用次数: 0

In vitro Antibacterial and Antifungal Activities of Sorindeia madagascariensis and Albizia harveyi Stem Barks 马达加斯加苍兰和哈氏合欢茎皮的体外抗菌和抗真菌活性

Pharmaceutical Sciences and Research Pub Date : 2022-05-18 DOI: 10.7454/psr.v9i1.1254

Barks, Paul Malaba Makoye, Sunday Mrutu, I. Daniel, M. Mbunde, J. Sempombe, V. Mugoyela

引用次数: 0

Antihypertensive and Antioxidant Activity of Herbal Medicine (Jamu) B on Rats Induced by 10% Fructose 中药Jamu B对10%果糖诱导大鼠的降压和抗氧化作用

Pharmaceutical Sciences and Research Pub Date : 2022-05-18 DOI: 10.7454/psr.v9i1.1252

Reza Pertiwi, Hermanus Ehe Hurit, Sri Rahayu, Y. Eden

{"title":"Antihypertensive and Antioxidant Activity of Herbal Medicine (Jamu) B on Rats Induced by 10% Fructose","authors":"Reza Pertiwi, Hermanus Ehe Hurit, Sri Rahayu, Y. Eden","doi":"10.7454/psr.v9i1.1252","DOIUrl":"https://doi.org/10.7454/psr.v9i1.1252","url":null,"abstract":"Prolonged fructose intake can activate the renin-angiotensin-aldosterone system and enhance oxidative stress conditions, causing hypertension and hyperuricemia. This study aimed to determine the antihypertensive and antioxidant activity of Jamu B on the activity of superoxide dismutase (SOD), level of glutathione (GSH), and malondialdehyde (MDA) in the kidneys of Sprague Dawley rats induced by 10% fructose. Thirty rats were divided into six groups. Groups B1-B3 were Jamu groups at a dose of 0.12 g/kg BW (B1), 0.24 g/kg BW (B2), and 0.36 g/kg BW (B3); group C (positive control) was given captopril 0.1 g/kg BW, group D (negative control) was given only 10% fructose, and group E (normal control) was healthy rats. The 10% fructose was induced for eight weeks and the treatment was given on the week 9 th and 10 th . Jamu B reduced systolic and diastolic blood pressure at all doses, but was not significantly different from the normal control group (p>0.05). The SOD activity in groups B1, B2, B3, and C was significantly different from the negative control group (p<0.05). However, GSH levels only showed a significant difference between B2, B3, C, and the negative control group (p<0.05). The MDA levels only showed a significant difference between B3 and the negative control group (p<0.05). Jamu B at the dose of 0.12 g/kg BW, 0.24 g/kg BW, and 0.36 g/kg BW have antihypertensive and antioxidant activity in the kidneys of rats induced by 10% fructose. Nevertheless, Jamu B at a dose of 0.36 g/kg BW was the most effective as an antihypertensive and antioxidant agent.","PeriodicalId":55754,"journal":{"name":"Pharmaceutical Sciences and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41448918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Environmental Pollutants Exposure with Pulmonary Fibrosis: A Mini Review of Molecular Mechanism Mediated 环境污染物暴露与肺纤维化的关系——介导的分子机制综述

Pharmaceutical Sciences and Research Pub Date : 2022-05-18 DOI: 10.7454/psr.v9i1.1243

Soni Siswanto, B. W. Wardhani

引用次数: 1

Identification of Community Knowledge on Lead Contamination and Analysis of Lead Level in Fish 社区铅污染知识的鉴定及鱼类铅含量分析

Pharmaceutical Sciences and Research Pub Date : 2022-05-18 DOI: 10.7454/psr.v9i1.1224

Sita Nurwulan, S. Damayanti, L. Amalia

{"title":"Identification of Community Knowledge on Lead Contamination and Analysis of Lead Level in Fish","authors":"Sita Nurwulan, S. Damayanti, L. Amalia","doi":"10.7454/psr.v9i1.1224","DOIUrl":"https://doi.org/10.7454/psr.v9i1.1224","url":null,"abstract":"Poisoning is an event of human exposure to poisonous substances that are harmful to the body at certain doses. Lead is a heavy metal that can cause toxicity, including neurotoxic effects, hypertension, digestive system disorders, bone and tooth growth disorders, immune system disorders, infertility, and fetal disorders. Fish is a source of nutrients that are often consumed by people, however can also accumulate lead. This study aimed to analyze the lead level in fish and identify the community knowledge level regarding lead contamination in fish. Identification of community knowledge levels regarding lead. Identification of community knowledge levels regarding lead contamination in fish was conducted on three hundred respondents consisting of School of Pharmacy (SF) Institut Teknologi Bandung (ITB) one hundred students, one hundred community, and one hundred housewives. This study also analyzed lead levels in six fish samples using Graphite Furnace Atomic Absorption Spectroscopy (GFAAS). As the result, SF ITB students had the highest level of knowledge in the definition of lead (97%), lead poisoning (98%), fish lead accumulation (75%), the poisoning effects of lead, and sources of lead. However, all of the respondents had a low level of knowledge regarding regulations about lead levels in fish. The lead levels analysis on fish showed that three out of six samples had lead levels that exceeded the limit of lead contamination (>0.2 g/kg). The study indicated that respondents’ knowledge regarding lead contamination in fish is still low and lead-contaminated fish is still being sold on the market.","PeriodicalId":55754,"journal":{"name":"Pharmaceutical Sciences and Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48272217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Heparinoid as Systemic Prophylactic Anticoagulants on COVID-19 Patient Mortality and Its Safety Profiles: A Systematic Review and Meta-Analysis 类肝素作为系统性预防性抗凝剂对COVID-19患者死亡率的影响及其安全性:一项系统综述和荟萃分析

Pharmaceutical Sciences and Research Pub Date : 2021-12-01 DOI: 10.7454/psr.v8i3.1230

Ariska Deffy Anggarany, M. Alkaff, Famila Takhwifa, H. Nufus, Diana Paramita

{"title":"The Effect of Heparinoid as Systemic Prophylactic Anticoagulants on COVID-19 Patient Mortality and Its Safety Profiles: A Systematic Review and Meta-Analysis","authors":"Ariska Deffy Anggarany, M. Alkaff, Famila Takhwifa, H. Nufus, Diana Paramita","doi":"10.7454/psr.v8i3.1230","DOIUrl":"https://doi.org/10.7454/psr.v8i3.1230","url":null,"abstract":"Coagulopathy is one of the complications of COVID-19 and is associated with a higher risk of mortality. However, evidence regarding the effectiveness and safety of anticoagulant therapy in various doses among COVID-19 patients is limited. This systematic review and meta-analysis aims to review and explore the effect of using heparinoids as a systemic anticoagulant at prophylactic doses on mortality in COVID-19 patients. Systematic searches were conducted of various databases (Pubmed, ScienceDirect, SpringerLink, Scopus, and ProQuest) covering the period 2019-2021. We assessed the quality of the articles using the STROBE checklist. Studies with a high risk of bias were excluded before pooled effect size was synthesized with 95% confidence intervals (CI) using random-effects models. From the 12 identified studies (N=8,968), six observational studies (N=7,176) were involved in the meta-analysis. The studies reviewed in the paper used a retrospective cohort design in various settings. The pooled effect size of mortality comparing prophylactic anticoagulant and no anticoagulant in three studies showed that there was an association between using prophylactic anticoagulant and a lower risk of in-hospital mortality (pooled OR= 0.47; 95% CI 0.19-0.76). A prophylactic dose of heparinoid anticoagulant was also associated with lower mortality (pooled OR= 0.51; 95% CI 0.21-0.82) and with lower bleeding events compared to intermediate-to-therapeutic dose anticoagulants. Administration of heparinoid anticoagulants at prophylactic doses was associated with reduced mortality risk in hospitalized COVID-19 patients. Due to the increased risk of bleeding with therapeutic doses, the use of prophylaxis anticoagulant is suggested in COVID-19 patients who are not critically ill.","PeriodicalId":55754,"journal":{"name":"Pharmaceutical Sciences and Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48962698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Propolis on Bone Quality and Cortical Bone Thickness of Ovariectomized Female Wistar White Rats as A Model for Osteoporosis 蜂胶对去卵巢雌性Wistar大鼠骨质疏松模型骨质量和骨皮质厚度的影响

Pharmaceutical Sciences and Research Pub Date : 2021-12-01 DOI: 10.7454/psr.v8i3.1214

Dian, Ayu, Juwita, Almahdy, Ahmadin, Fitri, Rachmaini, Rahmad, Abdillah, Rosalia Medisa Fatma

{"title":"Effect of Propolis on Bone Quality and Cortical Bone Thickness of Ovariectomized Female Wistar White Rats as A Model for Osteoporosis","authors":"Dian, Ayu, Juwita, Almahdy, Ahmadin, Fitri, Rachmaini, Rahmad, Abdillah, Rosalia Medisa Fatma","doi":"10.7454/psr.v8i3.1214","DOIUrl":"https://doi.org/10.7454/psr.v8i3.1214","url":null,"abstract":"Estrogen deficiency increases the rate of osteoporosis, especially in menopausal women, by altering the bone tissue microarchitecture. Propolis has compounds that could be used as an alternative therapy to treat estrogen deficiency and to protect against bone damage. This study aims to determine the effect of propolis on bone quality and cortical bone thickness of femoral metaphysis in ovariectomized female Wistar white rats as a model for menopausal osteoporosis. The rats were divided into five groups: negative control group (not subjected to ovariectomy), sham group (subjected to ovariectomy), and treatment groups that were subjected to ovariectomy and given propolis orally at a dose of 180 mg/ kg BW, 360 mg/kg BW, and 720 mg/kg BW for 30 days. Bone quality and cortical bone thickness testing were undertaken on the 31st day. The osteoblast and osteoclast cell examination was evaluated using an Olympus BX 51 light microscope at 400x magnification for bone quality and the Betaview program, Beta 3.1MP Sony Exmor CMOS Sensor camera at 40x magnification for cortical bone thickness. Data were analyzed using the one-way ANOVA and continued with Duncan’s multiple range tests. It was found that propolis had a significant effect on the ratio of osteoblast and femur bone osteoclasts (p0.05). The administration of propolis at a dose of 180 mg/kg BW, 360 mg/kg BW, and 720 mg/kg BW had an effect in decreasing the ratio of osteoblasts and metaphysical osteoclast cells of femoral metaphysics. However, propolis administration did not affect the thickness of the femoral metaphysical cortical bone.","PeriodicalId":55754,"journal":{"name":"Pharmaceutical Sciences and Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48243105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0