Atla-Alternatives To Laboratory Animals最新文献_第2页

Introducing the COST Action 'Improving the Quality of Biomedical Science with 3Rs Concepts' (IMPROVE). 介绍 "用 3R 概念提高生物医学科学质量"（IMPROVE）的 COST 行动。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-11-01 Epub Date: 2024-09-27 DOI: 10.1177/02611929241286024

Maria Kitsara, Merima Smajlhodžić-Deljo, Lejla Gurbeta Pokvic, Bettina Bert, Nataliia Bubalo, Sevilay Erden, Nuno Henrique Franco, Giuseppe Chirico, Jonathan Gómez Raja, Fernando Gonzalez-Uarquin, Annemarie Lang, Nicole Linklater, Sandra Mojsova, I Anna S Olsson, Ioanna Sandvig, Alexandra Schaffert, Marthe Schmit, Sophie Schober, Bogdan Sevastre, Doris Wilflingseder, Arti Ahluwalia, Winfried Neuhaus

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Conference Diary. 会议日记。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-11-01 Epub Date: 2024-10-08 DOI: 10.1177/02611929241288674

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Chemicals 2.0 and Why We Need to Bypass the Gold Standard in Regulatory Toxicology. 化学品 2.0 和为什么我们需要绕过毒物学监管的黄金标准》（Chemicals 2.0 and Why We Need to Bypass the Gold Standard in Regulatory Toxicology）。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-10-30 DOI: 10.1177/02611929241296328

Andrew P Worth, Elisabet Berggren, Pilar Prieto

{"title":"Chemicals 2.0 and Why We Need to Bypass the Gold Standard in Regulatory Toxicology.","authors":"Andrew P Worth, Elisabet Berggren, Pilar Prieto","doi":"10.1177/02611929241296328","DOIUrl":"https://doi.org/10.1177/02611929241296328","url":null,"abstract":"The use of adverse effect data from animals as the gold standard in regulatory toxicology has a long tradition dating back to the 1960s. It has also been increasingly criticised, based on both scientific and animal welfare concerns, and yet, animal studies remain the gold standard in most areas of toxicology to this very day. In the 1980s, when the first generation of non-animal methods were evaluated as alternatives to animal testing, it was logical to compare the 'new' data obtained with historical animal data. This worked reasonably well for simple endpoints, such as skin and eye irritation, but became problematic for the more complex systemic endpoints, since in these cases, the in vivo effects are not directly comparable to those observed in in vitro systems. While the need to redefine the gold standard is not new, there is still no consensus on how to do so. We propose a consistent principle that avoids the need for animal reference data, while also ensuring an equivalent or better level of protection. We argue that the gold standard can be redefined, or rather bypassed, by focusing on risk management outcomes rather than the outputs of animal methods. This allows us to more efficiently protect human health and the environment, ensuring the safe use of chemicals while also identifying less hazardous chemicals for use as substitutes. We describe how this might work out for two main contexts of use: classification and labelling, and risk assessment. This has implications for the implementation of the EU Commission Roadmap toward the phasing out of animal testing in chemical safety assessments.","PeriodicalId":55577,"journal":{"name":"Atla-Alternatives To Laboratory Animals","volume":" ","pages":"2611929241296328"},"PeriodicalIF":2.4,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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An Evaluation of the Replacement of Animal-derived Biomaterials in Human Primary Cell Culture. 评估人类原代细胞培养中动物源生物材料的替代性。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-09-01 Epub Date: 2024-08-09 DOI: 10.1177/02611929241269004

Laura R Bramwell, Samantha J Gould, Merlin Davies, Conor McMullan, Emily C Trusler, Lorna W Harries

{"title":"An Evaluation of the Replacement of Animal-derived Biomaterials in Human Primary Cell Culture.","authors":"Laura R Bramwell, Samantha J Gould, Merlin Davies, Conor McMullan, Emily C Trusler, Lorna W Harries","doi":"10.1177/02611929241269004","DOIUrl":"10.1177/02611929241269004","url":null,"abstract":"The likelihood that potential new drugs will successfully navigate the current translational pipeline is poor, with fewer than 10% of drug candidates making this transition successfully, even after their entry into clinical trials. Prior to this stage, candidate drugs are typically evaluated by using models of increasing complexity, beginning with basic in vitro cell culture studies and progressing through to animal studies, where many of these candidates are lost due to lack of efficacy or toxicology concerns. There are many reasons for this poor translation, but interspecies differences in functional and physiological parameters undoubtedly contribute to the problem. Improving the human-relevance of early preclinical in vitro models may help translatability, especially when targeting more nuanced species-specific cell processes. The aim of the current study was to define a set of guidelines for the effective transition of human primary cells of multiple lineages to more physiologically relevant, translatable, animal-free in vitro culture conditions. Animal-derived biomaterials (ADBs) were systematically replaced with non-animal-derived alternatives in the in vitro cell culture systems, and the impact of the substitutions subsequently assessed by comparing the kinetics and phenotypes of the cultured cells. ADBs were successfully eliminated from primary human dermal fibroblast, uterine fibroblast, pulmonary fibroblast, retinal endothelial cell and peripheral blood mononuclear cell culture systems, and the individual requirements of each cell subtype were defined to ensure the successful transition toward growth under animal-free culture conditions. We demonstrate that it is possible to transition ('humanise') a diverse set of human primary cell types by following a set of simple overarching principles that inform the selection, and guide the evaluation of new, improved, human-relevant in vitro culture conditions.","PeriodicalId":55577,"journal":{"name":"Atla-Alternatives To Laboratory Animals","volume":" ","pages":"247-260"},"PeriodicalIF":2.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141910166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conference Diary. 会议日记。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-09-01 DOI: 10.1177/02611929241274428

引用次数: 0

Development of Sensitive In Vitro Protocols for the Biocompatibility Testing of Medical Devices and Pharmaceuticals Intended for Contact with the Eyes: Acute Irritation and Phototoxicity Assessment. 开发用于与眼睛接触的医疗设备和药品生物兼容性测试的敏感体外协议：急性刺激和光毒性评估。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-09-01 Epub Date: 2024-08-21 DOI: 10.1177/02611929241270095

Peter Pôbiš, Júlia Kubalcová, Tatiana Milasová, Helena Kandárová

{"title":"Development of Sensitive In Vitro Protocols for the Biocompatibility Testing of Medical Devices and Pharmaceuticals Intended for Contact with the Eyes: Acute Irritation and Phototoxicity Assessment.","authors":"Peter Pôbiš, Júlia Kubalcová, Tatiana Milasová, Helena Kandárová","doi":"10.1177/02611929241270095","DOIUrl":"10.1177/02611929241270095","url":null,"abstract":"This study introduces a novel in vitro methodology that employs the 3-D reconstructed tissue model, EpiOcular, to assess the irritation and phototoxicity potential of medical devices and drugs in contact with the eye. Our study evaluated diverse test materials, including medical devices, ophthalmological solutions and an experimental drug (cemtirestat), for their potential to cause eye irritation and phototoxicity. The protocols used in this study with the EpiOcular tissue model were akin to those used in the ultra-mildness testing of cosmetic formulations, which is challenging to predict with standard in vivo rabbit tests. To design these protocols, we leveraged experience gained from the validation project on the EpiDerm skin irritation test for medical devices (ISO 10993-23:2021) and the OECD TG 498 method for photo-irritation testing. The predictions were based on the tissue viability and inflammatory response, as determined by IL-1α release. By developing and evaluating these protocols for medical devices, we aimed to expand the applicability domain of the tests referred to in ISO 10993-23. This will contribute to the standardisation and cost-effective safety evaluation of ophthalmic products, while reducing reliance on animal testing in this field. The findings obtained from the EpiOcular model in the photo-irritation test could support its implementation in the testing strategies outlined in OECD TG 498.","PeriodicalId":55577,"journal":{"name":"Atla-Alternatives To Laboratory Animals","volume":" ","pages":"261-275"},"PeriodicalIF":2.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial. 社论

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-09-01 Epub Date: 2024-08-07 DOI: 10.1177/02611929241274885

Judith C Madden

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Progress and Remaining Opportunities to Increase the Use of Animal-free Antibodies in the USA. 美国在增加使用无动物抗体方面取得的进展和仍然存在的机遇。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-09-01 Epub Date: 2024-07-24 DOI: 10.1177/02611929241266472

Katherine Groff, David Allen, Warren Casey, Amy J Clippinger

{"title":"Progress and Remaining Opportunities to Increase the Use of Animal-free Antibodies in the USA.","authors":"Katherine Groff, David Allen, Warren Casey, Amy J Clippinger","doi":"10.1177/02611929241266472","DOIUrl":"10.1177/02611929241266472","url":null,"abstract":"The scientific and ethical issues associated with the use of animal-derived antibodies in research can be overcome by the use of animal-free, sequence-defined recombinant antibodies, whose benefits are well documented. Here, we describe progress made following a 2019 expert meeting focused on improving the quality and reproducibility of biomedical research by accelerating the production and use of animal-free recombinant antibodies in the USA. In the five intervening years since the meeting, participants have established multifaceted initiatives to tackle the next steps outlined during the meeting. These initiatives include: prioritising the replacement of ascites-derived and polyclonal antibodies; distributing educational materials describing recombinant antibodies; fostering public-private partnerships to increase access to recombinant antibodies; and increasing the availability of funding for recombinant antibody development. Given the widescale use of antibodies across scientific disciplines, a transition to modern antibody production methods relies on a commitment from government agencies, universities, industry and funding organisations, to initiatives such as those outlined here.","PeriodicalId":55577,"journal":{"name":"Atla-Alternatives To Laboratory Animals","volume":" ","pages":"285-289"},"PeriodicalIF":2.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141753467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Conference Diary. 会议日记。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-07-01 Epub Date: 2024-06-12 DOI: 10.1177/02611929241260577

引用次数: 0

2023 Michael Balls Award. 2023 年迈克尔-鲍尔斯奖

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-07-01 DOI: 10.1177/02611929241262162

引用次数: 0