Aquatic Sciences最新文献

{"title":"Linking ancient refugia to modern diversity: evidence of multi-origin postglacial expansion of sockeye salmon on the Asian range","authors":"Anastasia M. Khrustaleva,&nbsp;Ekaterina V. Ponomareva,&nbsp;Grigorii N. Markevich","doi":"10.1007/s00027-026-01381-5","DOIUrl":"10.1007/s00027-026-01381-5","url":null,"abstract":"<div><p>Sockeye salmon (<i>Oncorhynchus nerka</i>) is a traditional object of fishery in the North Pacific, but its island populations are currently facing emerging threats from territorial development and escalating risks of unregulated fishing. The present study aims to assess the current status of Kuril Island populations determined by past biogeographical processes. We analyzed diversity distribution of the D-loop sequence (control region) and three mitochondrial single nucleotide polymorphisms (mtSNPs) localized in the <i>Cytb</i> and <i>COI</i> genes in sockeye salmon populations across the North Pacific. D-loop mtDNA variants were grouped into two distinct lineages: haplogroups 10T and 13T. Their distribution suggests an Asian origin for the 10T lineage and a North American origin for the 13T haplogroup. Testing the biogeographical scenarios supports the hypothesis of recurrent postglacial expansions of North American strains into the southernmost territories of the Asian range during the last two glacial cycles. Concurrently, during the Holocene transgression, there were two centers of sockeye salmon radiation in Asia associated with the refugium in the Kamchatka River basin and a minor cryptic refugium in the Hokkaido region. We also propose an “island bridge” hypothesis to explain dispersal of 10T-lineage from the Kamchatka River basin into Cook Inlet and Alaska Peninsula watersheds via the Aleutian Islands. These findings reveal complex trans-Pacific connectivity and historical demographic shifts, providing a crucial evolutionary framework for the management and preservation of peripheral sockeye salmon stocks.</p></div>","PeriodicalId":55489,"journal":{"name":"Aquatic Sciences","volume":"89 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149234832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potentially toxic cyanobacterial mats in tropical Mexican rivers: phylogeny of foundation cyanobacteria and associated microbiome composition","authors":"Aída Sánchez Salas,&nbsp;Angela Piedad Caro Borrero,&nbsp;Javier Carmona Jiménez,&nbsp;Kenia Márquez-Santamaria,&nbsp;Itzel Becerra Absalón","doi":"10.1007/s00027-026-01380-6","DOIUrl":"10.1007/s00027-026-01380-6","url":null,"abstract":"<div><p>Benthic cyanobacterial mats are complex microbial ecosystems in which cyanobacteria coexist with diverse associated microorganisms that contribute biogeochemical cycles and the production of secondary metabolites. This study aimed to identify the foundation cyanobacteria and associated bacterial communities of benthic mats and to evaluate the effects of environmental and biotic factors on potential toxin production in tropical mountain rivers of siliceous and calcareous basins. We integrated morphological characterization, 16S rRNA gene amplicon sequencing, cyanotoxin-related molecular analyses, high-performance liquid chromatography (HPLC)-based toxin detection, and environmental profiling of the study sites. We identified the foundation cyanobacteria as <i>Tychonema</i> sp., <i>Compactonostoc</i> sp., <i>Albertania</i> sp., and <i>Chamaesiphon mexicanus</i> in siliceous basins, and <i>Oxynema</i> sp. and <i>Ancylothrix</i> sp. in calcareous basins. Associated bacterial communities were dominated by Pseudomonadota and Bacillota, although their composition varied substantially among mats. Nitrogen availability was associated with differences in bacterial community composition, whereas temperature, pH, and conductivity were associated with patterns of alpha diversity. Cyanotoxin-related analyses revealed both potentially toxigenic and HPLC-confirmed toxigenic mats, including the detection of microcystins in mats associated with <i>Microcoleus</i> sp. and <i>Planktothrix agardhii</i>. Bacterial community composition did not differ significantly between potentially toxigenic and HPLC-confirmed toxigenic mats, although community dispersion differed between groups. Overall, the results demonstrate substantial environmental and microbial heterogeneity among benthic cyanobacterial mats and document the occurrence of cyanotoxins in tropical mountain rivers, providing a basis for a more detailed assessment of ecological and environmental factors, as well as mutualistic relationships, associated with the support and metabolic stratification of toxin-producing cyanospheres.</p></div>","PeriodicalId":55489,"journal":{"name":"Aquatic Sciences","volume":"89 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149234362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deforestation and monoculture expansion restructure zooplankton communities in neotropical reservoirs","authors":"Felipe Antonio dos Santos,&nbsp;Íttalo Luã Silva Medeiros,&nbsp;Cihelio Alves Amorim,&nbsp;Alef Jonathan da Silva,&nbsp;Camila Ângela Oliveira da Silva,&nbsp;Viviane Lúcia dos Santos Almeida de Melo,&nbsp;Mauro de Melo Júnior","doi":"10.1007/s00027-026-01377-1","DOIUrl":"10.1007/s00027-026-01377-1","url":null,"abstract":"<div><p>Deforestation and monoculture expansion in tropical watersheds represent important pressures on aquatic ecosystems, yet their associations with zooplankton communities in Neotropical reservoirs remain poorly understood. We evaluated how land use and land cover are associated with the distribution, diversity, composition, and structure of zooplankton communities in six tropical reservoirs with contrasting landscape configurations and trophic conditions. Samples were collected at 12 points per reservoir during dry and rainy periods by filtering 100 L of water. A total of 112 zooplankton species were recorded, comprising 60 Rotifera, 41 Cladocera, and 11 Copepoda, with the highest species richness occurring in the oligotrophic Siriji reservoir. Zooplankton diversity varied among reservoirs, while abundance-weighted diversity showed no consistent pattern between oligotrophic and hypereutrophic conditions. Rotifers, particularly representatives of Brachionidae, showed greater numerical dominance in hypereutrophic reservoirs. A total of 28 indicator species were identified (IndVal &gt; 70%; <i>p</i> &lt; 0.05), showing associations with land use and land cover, seasonality, trophic state, and limnological conditions. Zooplankton community composition differed significantly among reservoirs (permutational multivariate analysis of variance [PERMANOVA]: <i>F</i> = 57.611; <i>p</i> = 0.001), between seasonal periods (<i>F</i> = 172.245; <i>p</i> = 0.001), and for their interaction (<i>F</i> = 65.999; <i>p</i> = 0.001). Partial RDA explained 54.44% of the variation in zooplankton density, with limnological variables accounting for 24.1%, land use and land cover for 17.1%, and their shared fraction for 13.1%. Overall, zooplankton community structure was strongly associated with landscape configuration and related limnological conditions, highlighting the importance of riparian vegetation conservation for maintaining aquatic biodiversity.</p></div>","PeriodicalId":55489,"journal":{"name":"Aquatic Sciences","volume":"89 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00027-026-01377-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149235042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methane flux patterns following zooplankton addition in lake mesocosms with contrasting primary producers","authors":"Dingxin Zhang,&nbsp;Ailifeire Anwaier,&nbsp;Wenjuan Qiu,&nbsp;Fei Diao,&nbsp;Huihui Chen,&nbsp;Zhigang Mao,&nbsp;Xiaohong Gu,&nbsp;Yaling Su","doi":"10.1007/s00027-026-01383-3","DOIUrl":"10.1007/s00027-026-01383-3","url":null,"abstract":"<div><p>In lake ecosystems, different dominant primary producers may exert distinct effects on methane (CH₄) emissions. As key secondary producers and the principal grazers of phytoplankton and bacteria, zooplankton could modulate CH₄ cycling. However, comparative studies are currently lacking that evaluate the impacts of zooplankton on ecosystem CH₄ flux, and whether these impacts vary among systems containing different dominant primary-producer species. We posit that the influence of zooplankton on CH₄ emissions is modulated by the functional and structural attributes of the dominant primary producers. In this study, we conducted a mesocosm experiment to examine the individual and combined effects of three dominant primary-producer species (macrophyte-dominated: <i>Hydrilla verticillata</i>, <i>Vallisneria natans</i>; phytoplankton-dominated: <i>Chlorella pyrenoidosa</i>) and zooplankton addition on ecosystem CH₄ emissions. In treatments without zooplankton addition, <i>H. verticillata</i> mesocosms exhibited significantly higher CH₄ fluxes across the water–air interface (104.7 ± 50.5 μmol m⁻² day⁻¹) than those of <i>V. natans</i> mesocosms (78.2 ± 43.4 μmol m⁻² day⁻¹). <i>C. pyrenoidosa</i> mesocosms exhibited intermediate CH₄ fluxes, with no significant differences compared with either macrophyte treatment. Zooplankton addition was associated with higher CH₄ flux in the <i>H. verticillata</i> treatment (160.8 ± 126.5 μmol m⁻² day⁻¹), whereas no significant effect was detected in the <i>V. natans</i> or <i>C. pyrenoidosa</i> treatments. Correlation analyses revealed weak but significant positive relationships between CH₄ flux and both zooplankton biomass and <i>H. verticillata</i> biomass. Our findings suggest that lake CH₄ flux responses to zooplankton addition are influenced by the identity and associated traits of the dominant primary producer.</p></div>","PeriodicalId":55489,"journal":{"name":"Aquatic Sciences","volume":"89 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149234601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hidden competitor: overlooked Carassius species shows strong niche overlap with Nile tilapia","authors":"Million Tesfaye,&nbsp;Alebachew Mulat,&nbsp;Gashaw Tesfaye,&nbsp;Mojmír Vašek,&nbsp;Jan Kubečka,&nbsp;Travis B. Meador,&nbsp;Marek Šmejkal","doi":"10.1007/s00027-026-01378-0","DOIUrl":"10.1007/s00027-026-01378-0","url":null,"abstract":"<div><p>The ecological impacts of non-native species are a primary cause of global biodiversity loss, yet in complex, multi-invader systems, the role of individual species can be masked. In tropical Lake Ziway, Ethiopia, the fishery historically dominated by the native Nile tilapia (<i>Oreochromis niloticus</i>) has shifted markedly over the past 3 decades toward dominance by non-native species. Here, we investigated the trophic role of the Japanese gengorobuna (<i>Carassius cuvieri</i>), a non-native fish species whose presence for decades was undocumented because of its persistent misidentification as crucian carp (<i>Carassius carassius</i>). Using stable isotope analysis (<i>δ</i>¹³C and <i>δ</i>¹⁵N), we tested the hypothesis that this long-overlooked herbivorous fish would primarily compete with other functionally similar native and non-native species. Our results revealed an interesting and more concerning interaction. While <i>C. cuvieri</i> exhibited moderate niche overlap with common carp (<i>Cyprinus carpio</i>) and African catfish (<i>Clarias gariepinus</i>), its isotopic niche showed high and significant overlap with the native Nile tilapia (71.8% probabilistic overlap). We argue that this convergence is driven by the trophic plasticity and morphological adaptations of <i>C. cuvieri</i>, which allow it to efficiently exploit the same detrital and primary production resources on which the native tilapia depend. This study provides the first quantitative evidence that a long-established but previously misidentified non-native species is a significant and direct competitor to the most economically and ecologically important native fish species in the lake.</p></div>","PeriodicalId":55489,"journal":{"name":"Aquatic Sciences","volume":"89 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149234647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and distribution of cryptobenthic fishes and their association with epibenthic fauna in a subtropical estuary","authors":"B. M. Carvalho,&nbsp;R. Álvarez,&nbsp;G. P. da Silva,&nbsp;J. M. Serenato,&nbsp;R. Alitto,&nbsp;M. Borges,&nbsp;M. V. Gernet,&nbsp;M. Di Domenico","doi":"10.1007/s00027-026-01371-7","DOIUrl":"10.1007/s00027-026-01371-7","url":null,"abstract":"<div><p>Cryptobenthic fishes (CF) are cryptic species that exhibit mimicry with their benthic environment and can be either detritivorous or zoobenthivorous. Few studies have examined the influence of epibenthic macrofauna on CF diversity. This study analyzed the colonization and spatiotemporal succession of CF and macrofauna in fish-specific autonomous reef monitoring structures (FARMS) at Paranaguá Estuarine Complex (Brazil). Five FARMS were installed in localities in São Miguel, Amparo and Nova Brasília, which represented an estuarine gradient. Sampling was conducted every 2 months from October 2023 to August 2024; 303 individuals belonging to seven species associated with the FARMS were recorded, five of which were classified as CF. <i>Bathygobius soporator</i> was the dominant species in all of the sampled areas. To identify the epibenthic macrofauna, scrapings were performed every 2 months over the 10-month experiment from the FARMS installed in Nova Brasília and São Miguel. A dominance pattern of crustaceans, annelids and gastropods species was observed in Nova Brasília. In São Miguel, the macrofauna was dominated by annelids, bivalves, crustaceans, and ascidians. Individual FARMS were used as sampling replicates to evaluate spatial and temporal variation in fish assemblages. Fish community structure differed among areas, with a significant area × sampling event interaction indicating area-specific temporal trajectories, whereas no common temporal effect was detected. Macrofaunal assemblages differed between Nova Brasília and São Miguel, but no significant overall effect of fouling time was detected. Fish and macrofaunal community configurations were significantly concordant (procrustean randomization test, <i>r</i> = 0.636, <i>P</i> = 0.0348). These results indicate that cryptobenthic fish assemblages are spatially structured and that their temporal variation is associated with local changes in fouling communities, although the observational design did not demonstrate direct causal effects of macrofauna on fishes.</p></div>","PeriodicalId":55489,"journal":{"name":"Aquatic Sciences","volume":"89 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00027-026-01371-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149176543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trace metal accumulation and modulation of antioxidant biomarkers in native and invasive crabs from the Gulf of Gabes, Southern Mediterranean","authors":"Safa Bejaoui,&nbsp;Feriel Ghribi,&nbsp;Wafa Trabelsi,&nbsp;Dalya Belhassen,&nbsp;Imene Chetoui,&nbsp;Rym Baati,&nbsp;Nejla Soudani","doi":"10.1007/s00027-026-01374-4","DOIUrl":"10.1007/s00027-026-01374-4","url":null,"abstract":"<div><p>The proliferation of invasive species poses significant ecological and economic challenges, particularly in polluted coastal ecosystems where native species may be disproportionately affected. The aim of this study was to assess and compare the tolerance of the native crab <i>Carcinus aestuarii</i> and the invasive crab <i>Portunus segnis</i> to environmental stressors, by focusing on trace metal pollution in the Gulf of Gabes (Tunisia). Species-specific metal tolerance was evaluated using a multi-biomarker approach, including measurements of trace metal accumulation, alterations in proximate body composition and fatty acid profiles, macromolecular damage, and antioxidant defense responses in gill and muscle tissues. The results demonstrated that <i>C. aestuarii</i> exhibited greater sensitivity than <i>P. segnis</i>, with significantly higher trace metal accumulation, more pronounced macromolecular damage, and greater disruption of antioxidant defenses. In contrast, <i>P. segnis</i> showed enhanced physiological resilience, reflecting species-specific adaptive strategies. These findings highlight the importance of integrated biomarker assessments for monitoring pollution impacts and provide insights into the mechanisms underlying invasive species success. They also inform future research directions and management strategies aimed at mitigating the ecological consequences of invasive species in polluted coastal areas.</p></div>","PeriodicalId":55489,"journal":{"name":"Aquatic Sciences","volume":"88 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149189369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influencing pathways of meteorological factors on particulate organic carbon concentrations in Lake Hongze and its inflowing rivers","authors":"Qi Leng,&nbsp;ZiYue Li,&nbsp;FengTing Wu,&nbsp;Xiaozi Chang,&nbsp;ShengHao Zhang,&nbsp;JianLing Fan,&nbsp;XiaoLi Shi,&nbsp;YingXun Du","doi":"10.1007/s00027-026-01375-3","DOIUrl":"10.1007/s00027-026-01375-3","url":null,"abstract":"<div><p>Lakes play an important role in the regional and global carbon cycle, acting as both sources of carbon emissions and carbon sinks. The sedimentation of particulate organic carbon (POC) represents an important pathway for carbon sink in lakes, and variations in POC concentration are strongly regulated by environmental factors. In this study, we investigated the spatiotemporal variations of POC concentrations in Lake Hongze and its 13 inflowing rivers. Based on the relationships among meteorological factors, nutrients, chlorophyll <i>a</i>, and POC, a structural equation model was established to quantify the effect sizes of different influencing pathways. The results showed that POC concentrations exhibited distinct seasonal fluctuations, with significantly higher values in summer and autumn. POC concentrations in Lake Hongze were higher than those in Inner Mongolia–Xinjiang lakes and Tibetan Plateau lakes, and represented a medium-to-high level among Eastern Plain lakes. In inflowing rivers, precipitation affected POC not only by directly transporting allochthonous POC, but also by indirectly promoting autochthonous POC production through nutrient inputs; and temperature mainly increased POC by enhancing endogenous POC synthesis. Within Lake Hongze, precipitation delivered allochthonous organic matter and river-derived chlorophyll <i>a</i>, but had no significant effect on nutrient levels. In contrast, temperature increased lake POC mainly by accelerating nutrient release from sediments. These findings reveal distinct response mechanisms of POC to meteorological factors between inflowing rivers and the lake interior, providing a scientific basis for lake carbon sink management under climate change.</p></div>","PeriodicalId":55489,"journal":{"name":"Aquatic Sciences","volume":"88 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149188993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two pathways of essential polyunsaturated fatty acid supply from terrestrial invertebrates to fish in small shaded rivers","authors":"Olesia N. Makhutova,&nbsp;Elena V. Borisova,&nbsp;Yulia O. Mashonskaya","doi":"10.1007/s00027-026-01372-6","DOIUrl":"10.1007/s00027-026-01372-6","url":null,"abstract":"<div><p>In small shaded forest rivers, autochthonous primary production is limited, creating a deficiency of essential polyunsaturated fatty acids (PUFAs) for consumers. Previously, we demonstrated that allochthonous subsidy provides a significant flux of physiologically essential PUFAs into the shaded Krutaya Kacha River. Here, we aimed to reveal the specific terrestrial taxa driving this nutritional subsidy and compare them to aquatic taxa. Discriminant analysis separated aquatic from terrestrial taxa. Aquatic taxa, particularly Ephemeroptera, remained the primary source of physiologically valuable eicosapentaenoic acid (EPA; 20:5n‑3) for fish. Terrestrial phytophagous insects (Hemiptera, Homoptera, Lepidoptera, Hymenoptera, and Coleoptera) were rich in linoleic acid (LIN; 18:2n‑6) and/or alpha‑linolenic acid (ALA; 18:3n‑3) but contained very low levels of EPA and arachidonic acid (ARA; 20:4n‑6). By contrast, terrestrial predators and scavengers, especially Tetragnathidae (spiders) and Carabidae (carabid beetles), showed high EPA and ARA contents, comparable to those of aquatic insects. Spiders contained up to 10.6% EPA and 3.2% ARA, while carabid beetles contained up to 9.4% EPA and 4.0% ARA. Terrestrial invertebrates subsidized essential PUFAs via two discrete pathways: a direct pathway of LIN and/or ALA from terrestrial plants via phytophages and a “boomerang” pathway of ARA and EPA—from aquatic prey to terrestrial predators and scavengers and back into the river. These two pathways may be critical for essential lipid nutrition in shaded rivers occupying vast areas of boreal forests.</p></div>","PeriodicalId":55489,"journal":{"name":"Aquatic Sciences","volume":"88 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149189172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatiotemporal patterns of fish community composition and structure in a subtropical reservoir: comparison of eDNA metabarcoding and gillnetting","authors":"Dong-Hai Wu,&nbsp;Sai Wang,&nbsp;Peng-Fei Xie,&nbsp;Chu-Jun Zou,&nbsp;Hui-Long Ou,&nbsp;Tuan-Tuan Wang,&nbsp;Jia Xie,&nbsp;Yang Zhang,&nbsp;Cong-Cong Jin,&nbsp;Jie Feng,&nbsp;Shen-Hao Wu,&nbsp;Hina Amin,&nbsp;Sharon Indasi Lubembe,&nbsp;Naimat Ullah,&nbsp;Lei Liu","doi":"10.1007/s00027-026-01373-5","DOIUrl":"10.1007/s00027-026-01373-5","url":null,"abstract":"<div><p>Effective monitoring of fish communities is essential for the ecological management of aquatic ecosystems. However, in complex artificial freshwater habitats, the comparative efficacy of emerging noninvasive tools versus traditional methods remains underexplored. To assess the applicability of noninvasive monitoring tools, we compared environmental DNA (eDNA) metabarcoding with gillnetting for evaluating fish communities in the subtropical Xikeng Reservoir, southern China. A total of 38 fish species from seven orders and ten families were identified, with eDNA metabarcoding detecting more species (34 vs. 22), genera (31 vs. 21), and families (10 vs. 9) than gillnetting. The seasonal difference in fish community structure based on operational taxonomic unit (OTU) abundance (principal component analysis, <i>R</i> = 0.526) was stronger than that based on individual number (<i>R</i> = 0.276) and fresh weight (<i>R</i> = 0.149). Non-metric multidimensional scaling (NMDS) ordinations complemented by permutational multivariate analysis of variance (PERMANOVA) confirmed significant spatial segregation in fish community structures between littoral and limnetic zones across all datasets (<i>P</i> &lt; 0.001; OTU abundance: <i>F</i> = 24.620; individual number: <i>F</i> = 12.216; fresh weight: <i>F</i> = 10.256), with eDNA metabarcoding providing clearer habitat discrimination. eDNA metabarcoding is capable of identifying not only the key environmental factors (e.g., water temperature, dissolved oxygen, chlorophyll-<i>a</i>, total nitrogen, ammonia nitrogen, and biochemical oxygen demand) that affect fish species composition but also of explaining a greater degree of variance based on OTU abundance (46.60%) than those based on individual number (35.96%) and fresh weight (34.67%) obtained by gillnetting. Linear discriminant analysis selected 15, five, and five indicator species by OTU abundance, individual number, and fresh weight, respectively. The additional ten OTU-based indicator species (e.g., <i>Misgurnus anguillicaudatus</i>, <i>Paramisgurnus dabryanus</i>, and <i>Sarotherodon galilaeus</i>) could effectively distinguish the fish community in the littoral zone. Our results demonstrated that eDNA metabarcoding enhanced species detectability, resolved temporal and spatial community distribution characteristics with higher environmental sensitivity, and effectively characterized complex littoral microhabitats by identifying specific indicator species. In the future, integrating eDNA metabarcoding with gillnetting will play a crucial role in the field monitoring of aquatic biodiversity.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":55489,"journal":{"name":"Aquatic Sciences","volume":"88 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149189301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}