Briefings in Functional Genomics最新文献_第5页

Prediction of strand-specific and cell-type-specific G-quadruplexes based on high-resolution CUT&Tag data. 基于高分辨率 CUT&Tag 数据预测链特异性和细胞类型特异性 G-四重链。

IF 4 3区生物学

Briefings in Functional Genomics Pub Date : 2024-05-15 DOI: 10.1093/bfgp/elad024

Yizhi Cui, Hongzhi Liu, Yutong Ming, Zheng Zhang, Li Liu, Ruijun Liu

{"title":"Prediction of strand-specific and cell-type-specific G-quadruplexes based on high-resolution CUT&Tag data.","authors":"Yizhi Cui, Hongzhi Liu, Yutong Ming, Zheng Zhang, Li Liu, Ruijun Liu","doi":"10.1093/bfgp/elad024","DOIUrl":"10.1093/bfgp/elad024","url":null,"abstract":"G-quadruplex (G4), a non-classical deoxyribonucleic acid structure, is widely distributed in the genome and involved in various biological processes. In vivo, high-throughput sequencing has indicated that G4s are significantly enriched at functional regions in a cell-type-specific manner. Therefore, the prediction of G4s based on computational methods is necessary instead of the time-consuming and laborious experimental methods. Recently, G4 CUT&Tag has been developed to generate higher-resolution sequencing data than ChIP-seq, which provides more accurate training samples for model construction. In this paper, we present a new dataset construction method based on G4 CUT&Tag sequencing data and an XGBoost prediction model based on the machine learning boost method. The results show that our model performs well within and across cell types. Furthermore, sequence analysis indicates that the formation of G4 structure is greatly affected by the flanking sequences, and the GC content of the G4 flanking sequences is higher than non-G4. Moreover, we also identified G4 motifs in the high-resolution dataset, among which we found several motifs for known transcription factors (TFs), such as SP2 and BPC. These TFs may directly or indirectly affect the formation of the G4 structure.","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9683854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular insights on the origin and development of waxy genotypes in major crop plants. 主要作物蜡质基因型起源与发展的分子研究

IF 4 3区生物学

Briefings in Functional Genomics Pub Date : 2024-05-15 DOI: 10.1093/bfgp/elad035

Vikram S Gaur, Salej Sood, Carlos Guzmán, Kenneth M Olsen

{"title":"Molecular insights on the origin and development of waxy genotypes in major crop plants.","authors":"Vikram S Gaur, Salej Sood, Carlos Guzmán, Kenneth M Olsen","doi":"10.1093/bfgp/elad035","DOIUrl":"10.1093/bfgp/elad035","url":null,"abstract":"Starch is a significant ingredient of the seed endosperm with commercial importance in food and industry. Crop varieties with glutinous (waxy) grain characteristics, i.e. starch with high amylopectin and low amylose, hold longstanding cultural importance in some world regions and unique properties for industrial manufacture. The waxy character in many crop species is regulated by a single gene known as GBSSI (or waxy), which encodes the enzyme Granule Bound Starch Synthase1 with null or reduced activity. Several allelic variants of the waxy gene that contribute to varying levels of amylose content have been reported in different crop plants. Phylogenetic analysis of protein sequences and the genomic DNA encoding GBSSI of major cereals and recently sequenced millets and pseudo-cereals have shown that GBSSI orthologs form distinct clusters, each representing a separate crop lineage. With the rapidly increasing demand for waxy starch in food and non-food applications, conventional crop breeding techniques and modern crop improvement technologies such as gene silencing and genome editing have been deployed to develop new waxy crop cultivars. The advances in research on waxy alleles across different crops have unveiled new possibilities for modifying the synthesis of amylose and amylopectin starch, leading to the potential creation of customized crops in the future. This article presents molecular lines of evidence on the emergence of waxy genes in various crops, including their genesis and evolution, molecular structure, comparative analysis and breeding innovations.","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47913967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prediction of drug-protein interaction based on dual channel neural networks with attention mechanism. 基于注意机制的双通道神经网络预测药物与蛋白质的相互作用

IF 4 3区生物学

Briefings in Functional Genomics Pub Date : 2024-05-15 DOI: 10.1093/bfgp/elad037

Dayu Tan, Haijun Jiang, Haitao Li, Ying Xie, Yansen Su

{"title":"Prediction of drug-protein interaction based on dual channel neural networks with attention mechanism.","authors":"Dayu Tan, Haijun Jiang, Haitao Li, Ying Xie, Yansen Su","doi":"10.1093/bfgp/elad037","DOIUrl":"10.1093/bfgp/elad037","url":null,"abstract":"The precise identification of drug-protein inter action (DPI) can significantly speed up the drug discovery process. Bioassay methods are time-consuming and expensive to screen for each pair of drug proteins. Machine-learning-based methods cannot accurately predict a large number of DPIs. Compared with traditional computing methods, deep learning methods need less domain knowledge and have strong data learning ability. In this study, we construct a DPI prediction model based on dual channel neural networks with an efficient path attention mechanism, called DCA-DPI. The drug molecular graph and protein sequence are used as the data input of the model, and the residual graph neural network and the residual convolution network are used to learn the feature representation of the drug and protein, respectively, to obtain the feature vector of the drug and the hidden vector of protein. To get a more accurate protein feature vector, the weighted sum of the hidden vector of protein is applied using the neural attention mechanism. In the end, drug and protein vectors are concatenated and input into the full connection layer for classification. In order to evaluate the performance of DCA-DPI, three widely used public data, Human, C.elegans and DUD-E, are used in the experiment. The evaluation metrics values in the experiment are superior to other relevant methods. Experiments show that our model is efficient for DPI prediction.","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10112268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic signature analysis and survival prediction of esophageal cancer based on N6-methyladenosine associated lncRNAs. 基于N6-甲基腺苷相关lncRNA的食管癌预后特征分析和生存预测

IF 4 3区生物学

Briefings in Functional Genomics Pub Date : 2024-05-15 DOI: 10.1093/bfgp/elad028

Ting He, Zhipeng Gao, Ling Lin, Xu Zhang, Quan Zou

{"title":"Prognostic signature analysis and survival prediction of esophageal cancer based on N6-methyladenosine associated lncRNAs.","authors":"Ting He, Zhipeng Gao, Ling Lin, Xu Zhang, Quan Zou","doi":"10.1093/bfgp/elad028","DOIUrl":"10.1093/bfgp/elad028","url":null,"abstract":"Esophageal cancer (ESCA) has a bad prognosis. Long non-coding RNA (lncRNA) impacts on cell proliferation. However, the prognosis function of N6-methyladenosine (m6A)-associated lncRNAs (m6A-lncRNAs) in ESCA remains unknown. Univariate Cox analysis was applied to investigate prognosis related m6A-lncRNAs, based on which the samples were clustered. Wilcoxon rank and Chi-square tests were adopted to compare the clinical traits, survival, pathway activity and immune infiltration in different clusters where overall survival, clinical traits (N stage), tumor-invasive immune cells and pathway activity were found significantly different. Through least absolute shrinkage and selection operator and proportional hazard (Lasso-Cox) model, five m6A-lncRNAs were selected to construct the prognostic signature (m6A-lncSig) and risk score. To investigate the link between risk score and clinical traits or immunological microenvironments, Chi-square test and Spearman correlation analysis were utilized. Risk score was found connected with N stage, tumor stage, different clusters, macrophages M2, B cells naive and T cells CD4 memory resting. Risk score and tumor stage were found as independent prognostic variables. And the constructed nomogram model had high accuracy in predicting prognosis. The obtained m6A-lncSig could be taken as potential prognostic biomarker for ESCA patients. This study offers a theoretical foundation for clinical diagnosis and prognosis of ESCA.","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9886829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microscale marvels: unveiling the macroscopic significance of micropeptides in human health. 微观奇迹：揭示微肽对人类健康的宏观意义。

IF 4 3区生物学

Briefings in Functional Genomics Pub Date : 2024-05-05 DOI: 10.1093/bfgp/elae018

Deepyaman Das, Soumita Podder

引用次数: 0

Breast cancer prognosis through the use of multi-modal classifiers: current state of the art and the way forward 通过使用多模态分类器进行乳腺癌预后分析：技术现状与未来方向

IF 4 3区生物学

Briefings in Functional Genomics Pub Date : 2024-05-01 DOI: 10.1093/bfgp/elae015

Archana Mathur, Nikhilanand Arya, Kitsuchart Pasupa, Sriparna Saha, Sudeepa Roy Dey, Snehanshu Saha

引用次数: 0

Unveiling aging dynamics in the hematopoietic system insights from single-cell technologies. 单细胞技术揭示造血系统的衰老动态

IF 4 3区生物学

Briefings in Functional Genomics Pub Date : 2024-04-30 DOI: 10.1093/bfgp/elae019

Xinrong Jin, Ruohan Zhang, Yunqi Fu, Qiunan Zhu, Liquan Hong, Aiwei Wu, Hu Wang

{"title":"Unveiling aging dynamics in the hematopoietic system insights from single-cell technologies.","authors":"Xinrong Jin, Ruohan Zhang, Yunqi Fu, Qiunan Zhu, Liquan Hong, Aiwei Wu, Hu Wang","doi":"10.1093/bfgp/elae019","DOIUrl":"https://doi.org/10.1093/bfgp/elae019","url":null,"abstract":"As the demographic structure shifts towards an aging society, strategies aimed at slowing down or reversing the aging process become increasingly essential. Aging is a major predisposing factor for many chronic diseases in humans. The hematopoietic system, comprising blood cells and their associated bone marrow microenvironment, intricately participates in hematopoiesis, coagulation, immune regulation and other physiological phenomena. The aging process triggers various alterations within the hematopoietic system, serving as a spectrum of risk factors for hematopoietic disorders, including clonal hematopoiesis, immune senescence, myeloproliferative neoplasms and leukemia. The emerging single-cell technologies provide novel insights into age-related changes in the hematopoietic system. In this review, we summarize recent studies dissecting hematopoietic system aging using single-cell technologies. We discuss cellular changes occurring during aging in the hematopoietic system at the levels of the genomics, transcriptomics, epigenomics, proteomics, metabolomics and spatial multi-omics. Finally, we contemplate the future prospects of single-cell technologies, emphasizing the impact they may bring to the field of hematopoietic system aging research.","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140861721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in genetic techniques and functional genomics for enhancing crop traits and agricultural sustainability. 基因技术和功能基因组学在提高作物性状和农业可持续性方面的进步。

IF 4 3区生物学

Briefings in Functional Genomics Pub Date : 2024-04-28 DOI: 10.1093/bfgp/elae017

Surender Kumar, Anupama Singh, Chander Mohan Singh Bist, Munish Sharma

{"title":"Advancements in genetic techniques and functional genomics for enhancing crop traits and agricultural sustainability.","authors":"Surender Kumar, Anupama Singh, Chander Mohan Singh Bist, Munish Sharma","doi":"10.1093/bfgp/elae017","DOIUrl":"https://doi.org/10.1093/bfgp/elae017","url":null,"abstract":"Genetic variability is essential for the development of new crop varieties with economically beneficial traits. The traits can be inherited from wild relatives or induced through mutagenesis. Novel genetic elements can then be identified and new gene functions can be predicted. In this study, forward and reverse genetics approaches were described, in addition to their applications in modern crop improvement programs and functional genomics. By using heritable phenotypes and linked genetic markers, forward genetics searches for genes by using traditional genetic mapping and allele frequency estimation. Despite recent advances in sequencing technology, omics and computation, genetic redundancy remains a major challenge in forward genetics. By analyzing close-related genes, we will be able to dissect their functional redundancy and predict possible traits and gene activity patterns. In addition to these predictions, sophisticated reverse gene editing tools can be used to verify them, including TILLING, targeted insertional mutagenesis, gene silencing, gene targeting and genome editing. By using gene knock-down, knock-up and knock-out strategies, these tools are able to detect genetic changes in cells. In addition, epigenome analysis and editing enable the development of novel traits in existing crop cultivars without affecting their genetic makeup by increasing epiallelic variants. Our understanding of gene functions and molecular dynamics of various biological phenomena has been revised by all of these findings. The study also identifies novel genetic targets in crop species to improve yields and stress tolerances through conventional and non-conventional methods. In this article, genetic techniques and functional genomics are specifically discussed and assessed for their potential in crop improvement.","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140873722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Short-homology-mediated PCR-based method for gene introduction in the fission yeast Schizosaccharomyces pombe 基于短同源物介导的 PCR 方法在裂殖酵母中引入基因

IF 4 3区生物学

Briefings in Functional Genomics Pub Date : 2024-04-26 DOI: 10.1093/bfgp/elae016

Cai-Xia Zhang, Ying-Chun Hou

{"title":"Short-homology-mediated PCR-based method for gene introduction in the fission yeast Schizosaccharomyces pombe","authors":"Cai-Xia Zhang, Ying-Chun Hou","doi":"10.1093/bfgp/elae016","DOIUrl":"https://doi.org/10.1093/bfgp/elae016","url":null,"abstract":"Schizosaccharomyces pombe is a commonly utilized model organism for studying various aspects of eukaryotic cell physiology. One reason for its widespread use as an experimental system is the ease of genetic manipulations, leveraging the natural homology-targeted repair mechanism to accurately modify the genome. We conducted a study to assess the feasibility and efficiency of directly introducing exogenous genes into the fission yeast S. pombe using Polymerase Chain Reaction (PCR) with short-homology flanking sequences. Specifically, we amplified the NatMX6 gene (which provides resistance to nourseothricin) using PCR with oligonucleotides that had short flanking regions of 20 bp, 40 bp, 60 bp and 80 bp to the target gene. By using this purified PCR product, we successfully introduced the NatMX6 gene at position 171 385 on chromosome III in S. pombe. We have made a simple modification to the transformation procedure, resulting in a significant increase in transformation efficiency by at least 5-fold. The success rate of gene integration at the target position varied between 20% and 50% depending on the length of the flanking regions. Additionally, we discovered that the addition of dimethyl sulfoxide and boiled carrier DNA increased the number of transformants by ~60- and 3-fold, respectively. Furthermore, we found that the removal of the pku70+ gene improved the transformation efficiency to ~5% and reduced the formation of small background colonies. Overall, our results demonstrate that with this modified method, even very short stretches of homologous regions (as short as 20 bp) can be used to effectively target genes at a high frequency in S. pombe. This finding greatly facilitates the introduction of exogenous genes in this organism.","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140798721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome structural dynamics: insights from Gaussian network analysis of Hi-C data. 基因组结构动力学：从对 Hi-C 数据的高斯网络分析中获得的启示。

IF 4 3区生物学

Briefings in Functional Genomics Pub Date : 2024-04-22 DOI: 10.1093/bfgp/elae014

Anupam Banerjee, She Zhang, Ivet Bahar

{"title":"Genome structural dynamics: insights from Gaussian network analysis of Hi-C data.","authors":"Anupam Banerjee, She Zhang, Ivet Bahar","doi":"10.1093/bfgp/elae014","DOIUrl":"https://doi.org/10.1093/bfgp/elae014","url":null,"abstract":"Characterization of the spatiotemporal properties of the chromatin is essential to gaining insights into the physical bases of gene co-expression, transcriptional regulation and epigenetic modifications. The Gaussian network model (GNM) has proven in recent work to serve as a useful tool for modeling chromatin structural dynamics, using as input high-throughput chromosome conformation capture data. We focus here on the exploration of the collective dynamics of chromosomal structures at hierarchical levels of resolution, from single gene loci to topologically associating domains or entire chromosomes. The GNM permits us to identify long-range interactions between gene loci, shedding light on the role of cross-correlations between distal regions of the chromosomes in regulating gene expression. Notably, GNM analysis performed across diverse cell lines highlights the conservation of the global/cooperative movements of the chromatin across different types of cells. Variations driven by localized couplings between genomic loci, on the other hand, underlie cell differentiation, underscoring the significance of the four-dimensional properties of the genome in defining cellular identity. Finally, we demonstrate the close relation between the cell type-dependent mobility profiles of gene loci and their gene expression patterns, providing a clear demonstration of the role of chromosomal 4D features in defining cell-specific differential expression of genes.","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140676888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0