Journal of Horticultural Science & Biotechnology最新文献_第2页

Effect of microclimate and photosynthesis on strawberry reproductive growth in a greenhouse: using cumulative leaf photosynthesis as an index to predict the time of harvest 小气候和光合作用对温室草莓生殖生长的影响——以叶片累积光合作用为指标预测收获期

4区农林科学

Journal of Horticultural Science & Biotechnology Pub Date : 2023-09-10 DOI: 10.1080/14620316.2023.2254759

Shintaro Ono, Daisuke Yasutake, Kensuke Kimura, I Kengo, Yoshiya Teruya, Kota Hidaka, Gaku Yokoyama, Tomoyoshi Hirota, Masaharu Kitano, Takashi Okayasu, Yukio Ozaki

{"title":"Effect of microclimate and photosynthesis on strawberry reproductive growth in a greenhouse: using cumulative leaf photosynthesis as an index to predict the time of harvest","authors":"Shintaro Ono, Daisuke Yasutake, Kensuke Kimura, I Kengo, Yoshiya Teruya, Kota Hidaka, Gaku Yokoyama, Tomoyoshi Hirota, Masaharu Kitano, Takashi Okayasu, Yukio Ozaki","doi":"10.1080/14620316.2023.2254759","DOIUrl":"https://doi.org/10.1080/14620316.2023.2254759","url":null,"abstract":"We evaluate whether cumulative photosynthesis (An,C) could predict the time of harvest in strawberry better than light (IC) or temperature (Ta,C) as forecast indices. We monitored the daily dynamics of flowering, fruit set, and harvest in over 2,700 fruits across the growing season in a greenhouse in Japan. Photosynthesis was calculated using a biochemical model, and An,C calculated by totalling the cumulative rate from flowering to harvest in each fruit. IC and Ta,C were calculated by totalling daily cumulative light intensity and average temperature. A new index (D) was defined as the normalised variation of An,C, IC, and Ta,C, and a statistical analysis was conducted on the means for each index. The results showed that An,C had the lowest D (0.114), suggesting that it was a better index for predicting the time of harvest. This study suggests the importance of considering the accumulation of photosynthates in individual fruit, which cannot be achieved by IC or Ta,C, these results can be used to improve the forecasting and optimisation of yield. However, further research is required to substantiate these claims.","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136071456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective Agrobacterium-mediated genetic transformation of okra (Abelmoschus esculentus L.) and generation of RNAi plants resistant to Begomovirus infecting okra 农杆菌介导的秋葵有效遗传转化及抗秋葵begomschus esculentus L. RNAi植株的生成

4区农林科学

Journal of Horticultural Science & Biotechnology Pub Date : 2023-09-10 DOI: 10.1080/14620316.2023.2251978

Nity Sharma, Navraj Kaur Sarao, Prashant Mohanpuria, Abhishek Sharma

{"title":"Effective Agrobacterium-mediated genetic transformation of okra (Abelmoschus esculentus L.) and generation of RNAi plants resistant to Begomovirus infecting okra","authors":"Nity Sharma, Navraj Kaur Sarao, Prashant Mohanpuria, Abhishek Sharma","doi":"10.1080/14620316.2023.2251978","DOIUrl":"https://doi.org/10.1080/14620316.2023.2251978","url":null,"abstract":"Okra is an important vegetable crop of the Malvaceae family and is infected by varying numbers of viruses of the genus Begomovirus. Regardless of the importance of the crop, very little consideration has been given to its genetic improvement. RNA interference (RNAi), a potent biotechnological tool, is known to control Begomovirus in many crops. For the implementation of successful RNAi, there is a need for an efficient genetic transformation system in okra. In the present study, we developed a procedure for Agrobacterium-mediated tissue culture–dependent regeneration of okra plants for the application of RNAi. Eleven transgenic okra RNAi plants were regenerated by utilising hypocotyls as explants. Transformed plants were screened with hygromycin at the regeneration stage and the presence of transgenes (AC1, AC2 & AC4 codes for replication-associated protein, transcriptional activator protein and suppressor of PTGS) in putative transformed plants was confirmed by polymerase chain reaction (PCR). Wild and transgenic lines were challenged with a dimeric Begomovirus clone or viruliferous whiteflies and the level of resistance was estimated with quantitative real-time reverse transcriptase PCR (qRT-PCR) by utilising viral gene–specific primers. The resistant transgenic lines accumulated very low titres of viral gene products according to the qRT-PCR assays compared to the control plants. This is the first report of tissue culture–mediated RNAi-derived resistance in okra against Begomovirus infection.","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136071443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The characterisation of phytoene synthase-1 and 2, and 1-D-deoxy-xylulose 5-phosphate synthase genes from red chilli pepper (Capsicum annuum) 红辣椒植物烯合成酶-1、2和1- d -脱氧木糖5-磷酸合成酶基因的鉴定

4区农林科学

Journal of Horticultural Science & Biotechnology Pub Date : 2023-05-17 DOI: 10.1080/14620316.2023.2211593

Harriet M. Berry, Nan Zhou, Daniel V. Rickett, Charles J. Baxter, Eugenia M. A. Enfissi, Paul D. Fraser

{"title":"The characterisation of phytoene synthase-1 and 2, and 1-D-deoxy-xylulose 5-phosphate synthase genes from red chilli pepper (Capsicum annuum)","authors":"Harriet M. Berry, Nan Zhou, Daniel V. Rickett, Charles J. Baxter, Eugenia M. A. Enfissi, Paul D. Fraser","doi":"10.1080/14620316.2023.2211593","DOIUrl":"https://doi.org/10.1080/14620316.2023.2211593","url":null,"abstract":"The red colouration of ripe chilli pepper fruit is due to the presence of the carotenoid capsanthin. 1-D-deoxy-xylulose 5-phosphate synthase (DXS) and phytoene synthase(s) (PSY) are influential biosynthetic steps in the carotenoid pathway. A panel of chilli accessions for varying fruit colour intensity revealed the correlation of carotenoid content with PSY and DXS transcript levels. The PSY and DXS genes were sequenced from high and low carotenoid genotypes to deduce potential allelic variation. PSY-1 and -2 showed tissue-specific expression, with PSY-1 and -2 expressed in chromoplast and chloroplast containing tissues, respectively. Protein modelling, phylogenetic analysis and transcription factor analysis were conducted to understand how the allelic variation identified could affect carotenoid biosynthesis and regulation, and subsequently colour intensity phenotype. A candidate mutation located in the transit peptide of the PSY-1 protein sequence was identified in the low colour intensity genotype. Sequence variation within the promoter region of the DXS gene present in the high colour intensity genotype was determined in the glyceraldehyde 3-phosphate binding motif (GAPF). These data can be exploited to develop tools and resources for the breeding of high colour intensity chilli pepper fruit.","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135862202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome analysis of Lilium Oriental × Trumpet hybrid roots reveals auxin-related genes and stress-related genes involved in picloram-induced somatic embryogenesis induction 对东方百合×小喇叭杂交根的转录组分析揭示了生长素相关基因和胁迫相关基因参与皮氯兰诱导体细胞胚胎发生的过程

IF 1.9 4区农林科学

Journal of Horticultural Science & Biotechnology Pub Date : 2019-05-04 DOI: 10.1080/14620316.2018.1500086

Chen Minmin, Zhang Jianjun, Zhou Yin, Liu Shuigen, Fang Xiaofen, Y. Liuyan, Guanshen Yuan, Zhang Yongchun

{"title":"Transcriptome analysis of Lilium Oriental × Trumpet hybrid roots reveals auxin-related genes and stress-related genes involved in picloram-induced somatic embryogenesis induction","authors":"Chen Minmin, Zhang Jianjun, Zhou Yin, Liu Shuigen, Fang Xiaofen, Y. Liuyan, Guanshen Yuan, Zhang Yongchun","doi":"10.1080/14620316.2018.1500086","DOIUrl":"https://doi.org/10.1080/14620316.2018.1500086","url":null,"abstract":"ABSTRACTHighly efficient somatic embryogenesis (SE) initiation systems are crucial for the clonal propagation and genetic transformation of Lilium Oriental × Trumpet hybrid. To elucidate the underlying mechanism of SE induction, we conducted de novo transcriptome sequencing of the roots at four different times in relation to SE initiation (0 days, control; 20 days, embryogenic callus induction (ECI) 1; 40 days, ECI2; 55 days, ECI3). A total of 4621, 7582, and 7427 differentially expressed genes (DEGs) were identified from the ECI1 vs. control, ECI2 vs. control, and ECI3 vs. control comparisons, respectively. The gene-enrichment analysis revealed 54 significant DEGs. Further analysis showed that the transcript levels of the auxin-related genes ARF9/15/16, the abscisic acid-related (ABA-related) genes PYL4/9, and stress-related transcription factors, including WRKY26, MYB36, NAC100, bHLH2/5/18/112, and HSP90, were increased at the ECI1 stage. ABI5 can influence the expression of stress-responsive genes as a...","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2019-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82335597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Molecular cloning of potassium transporter gene, MaHAK5 of mulberry (Morus alba L.) and gene expression and biochemistry analysis under potassium stress 桑钾转运蛋白基因MaHAK5的分子克隆及其在钾胁迫下的表达和生化分析

IF 1.9 4区农林科学

Journal of Horticultural Science & Biotechnology Pub Date : 2019-01-02 DOI: 10.1080/14620316.2018.1470909

Dandan Chen, Ackon Justice Afriyie, Ruixue Li, Kotoka Dominic, Long Li, Rongfang Li, Weiguo Zhao

引用次数: 5

Silicon promotes seedling growth and alters endogenous IAA, GA3 and ABA concentrations in Glycyrrhiza uralensis under 100 mM NaCl stress 在100mM NaCl胁迫下，硅促进甘草幼苗生长并改变其内源IAA、GA3和ABA浓度

IF 1.9 4区农林科学

Journal of Horticultural Science & Biotechnology Pub Date : 2019-01-02 DOI: 10.1080/14620316.2018.1450097

D. Lang, P. Fei, G. Cao, X. Jia, Y. T. Li, X. Zhang

{"title":"Silicon promotes seedling growth and alters endogenous IAA, GA3 and ABA concentrations in Glycyrrhiza uralensis under 100 mM NaCl stress","authors":"D. Lang, P. Fei, G. Cao, X. Jia, Y. T. Li, X. Zhang","doi":"10.1080/14620316.2018.1450097","DOIUrl":"https://doi.org/10.1080/14620316.2018.1450097","url":null,"abstract":"ABSTRACTA sand culture experiment was conducted to determine the effects of Si on the growth and endogenous hormone concentrations (indole-3-acetic acid [IAA], gibberellic acid [GA3], and abscisic acid [ABA]) in Glycyrrhiza uralensis seedlings under 100 mM NaCl stress. Si time-dependently improved the growth and increased the biomass of seedlings, and the effect was greater at 20 days than that at 10 days after Si treatment. The effects of Si on the growth parameters and biomass were dependent on Si concentration, that is, these parameters increased with increasing Si concentration. Si also increased the levels of endogenous hormones in G. uralensis seedlings in a concentration-dependent manner. The effects on IAA and GA3 increased with increasing Si concentrations, and the effect on ABA was higher at 2 mM Si than that at the other Si concentrations. Furthermore, the effects of Si on IAA and GA3 were greater at 10 days than those at 20 days after the treatment, whereas the effect on ABA was greater at 20 ...","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2019-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/14620316.2018.1450097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41875677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Genetics and molecular mapping of gynoecious (F) locus in cucumber (Cucumis sativus L.) 黄瓜雌核（F）基因座的遗传与分子定位

IF 1.9 4区农林科学

Journal of Horticultural Science & Biotechnology Pub Date : 2019-01-02 DOI: 10.1080/14620316.2018.1449671

G. Jat, A. D. Munshi, T. Behera, H. Choudhary, P. Dash, Amarnath Ravindran, S. Kumari

{"title":"Genetics and molecular mapping of gynoecious (F) locus in cucumber (Cucumis sativus L.)","authors":"G. Jat, A. D. Munshi, T. Behera, H. Choudhary, P. Dash, Amarnath Ravindran, S. Kumari","doi":"10.1080/14620316.2018.1449671","DOIUrl":"https://doi.org/10.1080/14620316.2018.1449671","url":null,"abstract":"Gynoecious is an important economic trait of cucumber for determinant of earliness and yield, yet genetic mechanism is not well understood for this trait. The experiment was conducted using F₂ mapping population by crossing of PPC-2, a gynoecious and parthenocarpic line with Pusa Uday (monoecious and non-parthenocarpic cultivar). Out of 179 SSR markers screened, 39 markers differentiated the gynoecious and monoecious parents. However, only 17 markers were segregating with F₂ mapping population, those were used for genotyping and linkage map analysis and these markers were placed along with F locus on chromosome 6 covering a total distance of 100.4cM. The SSR markers, SSR13251 and UW020605 were found to be closely linked to gynoecious (F) locus at 1.0 and 4.5 cM, respectively. The segregation of F₂ population of PPC-2 × Pusa Uday and GPC-1 × Punjab Naveen and test crosses for sex type herein suggested that single dominant gene controlled the gynoecious sex expression in cucumber particularly in gynoecious genotypes PPC-2 and GPC-1. Therefore, the monogenic dominant nature of gynoecious sex identified in the present experiment and SSR markers closely linked to the F locus will be useful in marker-assisted backcross breeding for transfering gynoecious trait into horticulturally desirable varieties.","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2019-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/14620316.2018.1449671","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49358475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Growth and development of Easter lily in response to container substrate with biochar 生物炭容器基质对复活节百合生长发育的影响

IF 1.9 4区农林科学

Journal of Horticultural Science & Biotechnology Pub Date : 2019-01-02 DOI: 10.1080/14620316.2018.1444514

Yan-Lan Guo, G. Niu, T. Starman, M. Gu

引用次数: 15

Brassinolides have a small effect on vegetative growth, and increase reproductive growth of pistachio trees Cv. Owhadi 油菜素内酯对开心果树的营养生长影响较小，但对其生殖生长有促进作用。Owhadi

IF 1.9 4区农林科学

Journal of Horticultural Science & Biotechnology Pub Date : 2019-01-02 DOI: 10.1080/14620316.2018.1450647

Z. Pakkish, M. R. H. Poor, M. A. Nasab, H. Asghari

引用次数: 5

Acquisition of triploid germplasms by controlled hybridisation between diploid and tetraploid in Chinese jujube 枣树二倍体与四倍体控制杂交获得三倍体种质

IF 1.9 4区农林科学

Journal of Horticultural Science & Biotechnology Pub Date : 2019-01-02 DOI: 10.1080/14620316.2018.1454266

F. Yan, Lihu Wang, Xingjuan Zheng, Zhi Luo, Jiurui Wang, Mengjun Liu

{"title":"Acquisition of triploid germplasms by controlled hybridisation between diploid and tetraploid in Chinese jujube","authors":"F. Yan, Lihu Wang, Xingjuan Zheng, Zhi Luo, Jiurui Wang, Mengjun Liu","doi":"10.1080/14620316.2018.1454266","DOIUrl":"https://doi.org/10.1080/14620316.2018.1454266","url":null,"abstract":"ABSTRACT‘Chenguang’ is the first released tetraploid cultivar (2n = 4x = 48) of Chinese jujube (Ziziphus jujuba Mill.) induced by colchicine. The purpose of this study was to create triploid germplasms by using ‘Chenguang’ as a parent. The controlled crosses between autotetraploid ‘Chenguang’ and diploid ‘Dongzao’ were carried out from 2013 to 2015 by maintaining the 2 parents in a nylon net and employing bees as pollinator. A total of 606 fruits were produced, yet only 17 seeds were obtained from ‘Dongzao’ and none from ‘Chenguang’. Direct sowing of the seeds in greenhouse showed a higher seedling rate than embryo culture. Four progeny plants were eventually obtained in the greenhouse and all of them were confirmed as authentic hybrids by SSR marker technique. At last, 3 triploids and 1 diploid were identified by flow cytometric analysis. Consequently, tetraploid ‘Chenguang’ with high pollen vitality and pollen number per anther could be used as a reliable 2n pollen provider, and the 2x × 4x cross combin...","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2019-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/14620316.2018.1454266","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46704858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6