Harriet M. Berry, Nan Zhou, Daniel V. Rickett, Charles J. Baxter, Eugenia M. A. Enfissi, Paul D. Fraser
{"title":"The characterisation of phytoene synthase-1 and 2, and 1-D-deoxy-xylulose 5-phosphate synthase genes from red chilli pepper (<i>Capsicum annuum</i>)","authors":"Harriet M. Berry, Nan Zhou, Daniel V. Rickett, Charles J. Baxter, Eugenia M. A. Enfissi, Paul D. Fraser","doi":"10.1080/14620316.2023.2211593","DOIUrl":null,"url":null,"abstract":"The red colouration of ripe chilli pepper fruit is due to the presence of the carotenoid capsanthin. 1-D-deoxy-xylulose 5-phosphate synthase (DXS) and phytoene synthase(s) (PSY) are influential biosynthetic steps in the carotenoid pathway. A panel of chilli accessions for varying fruit colour intensity revealed the correlation of carotenoid content with PSY and DXS transcript levels. The PSY and DXS genes were sequenced from high and low carotenoid genotypes to deduce potential allelic variation. PSY-1 and -2 showed tissue-specific expression, with PSY-1 and -2 expressed in chromoplast and chloroplast containing tissues, respectively. Protein modelling, phylogenetic analysis and transcription factor analysis were conducted to understand how the allelic variation identified could affect carotenoid biosynthesis and regulation, and subsequently colour intensity phenotype. A candidate mutation located in the transit peptide of the PSY-1 protein sequence was identified in the low colour intensity genotype. Sequence variation within the promoter region of the DXS gene present in the high colour intensity genotype was determined in the glyceraldehyde 3-phosphate binding motif (GAPF). These data can be exploited to develop tools and resources for the breeding of high colour intensity chilli pepper fruit.","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Horticultural Science & Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/14620316.2023.2211593","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"HORTICULTURE","Score":null,"Total":0}
引用次数: 0
Abstract
The red colouration of ripe chilli pepper fruit is due to the presence of the carotenoid capsanthin. 1-D-deoxy-xylulose 5-phosphate synthase (DXS) and phytoene synthase(s) (PSY) are influential biosynthetic steps in the carotenoid pathway. A panel of chilli accessions for varying fruit colour intensity revealed the correlation of carotenoid content with PSY and DXS transcript levels. The PSY and DXS genes were sequenced from high and low carotenoid genotypes to deduce potential allelic variation. PSY-1 and -2 showed tissue-specific expression, with PSY-1 and -2 expressed in chromoplast and chloroplast containing tissues, respectively. Protein modelling, phylogenetic analysis and transcription factor analysis were conducted to understand how the allelic variation identified could affect carotenoid biosynthesis and regulation, and subsequently colour intensity phenotype. A candidate mutation located in the transit peptide of the PSY-1 protein sequence was identified in the low colour intensity genotype. Sequence variation within the promoter region of the DXS gene present in the high colour intensity genotype was determined in the glyceraldehyde 3-phosphate binding motif (GAPF). These data can be exploited to develop tools and resources for the breeding of high colour intensity chilli pepper fruit.
成熟辣椒果实的红色是由于类胡萝卜素辣椒素的存在。1- d -脱氧木糖5-磷酸合成酶(DXS)和植物烯合成酶(PSY)是类胡萝卜素途径中重要的生物合成步骤。一组不同果实颜色强度的辣椒材料揭示了类胡萝卜素含量与PSY和DXS转录水平的相关性。对高、低类胡萝卜素基因型的PSY和DXS基因进行测序,推断潜在的等位基因变异。PSY-1和psy -2表现出组织特异性表达,PSY-1和psy -2分别在含染色质和含叶绿体的组织中表达。通过蛋白质建模、系统发育分析和转录因子分析来了解所鉴定的等位基因变异如何影响类胡萝卜素的生物合成和调控,以及随后的颜色强度表型。在低颜色强度基因型中发现了一个位于PSY-1蛋白序列传递肽的候选突变。高颜色强度基因型DXS基因启动子区域的序列变异在甘油醛3-磷酸结合基序(GAPF)中确定。这些数据可用于开发高色强辣椒果实育种的工具和资源。
期刊介绍:
The Journal of Horticultural Science and Biotechnology is an international, peer-reviewed journal, which publishes original research contributions into the production, improvement and utilisation of horticultural crops. It aims to provide scientific knowledge of interest to those engaged in scientific research and the practice of horticulture. The scope of the journal includes studies on fruit and other perennial crops, vegetables and ornamentals grown in temperate or tropical regions and their use in commercial, amenity or urban horticulture. Papers, including reviews, that give new insights into plant and crop growth, yield, quality and response to the environment, are welcome, including those arising from technological innovation and developments in crop genome sequencing and other biotechnological advances.