Revista Cientifica-Facultad De Ciencias Veterinarias最新文献

{"title":"Survival of a mixed culture of microencapsulated probiotic strains against the gastrointestinal barrier in vitro","authors":"Luz Alba Caballero–Pérez, Rene Tejedor–Arias, Elaysa Josefina Salas–Osorio","doi":"10.52973/rcfcv-e33296","DOIUrl":"https://doi.org/10.52973/rcfcv-e33296","url":null,"abstract":"Encapsulating materials preserve the viability of probiotics under gastrointestinal conditions. The aim of the research was to evaluate the protective effect of an encapsulating matrix, composed for the first time with three prebiotic materials to maintain the viability of a mixed culture of spray–dried microencapsulated probiotics under simulated gastrointestinal and prebiotic conditions. Microcapsules of four formulations with better viability were then evaluated by inoculating microencapsulated and free strains in MRS broth, adjusting three pH values, bile salts, broth with and without carbohydrate (prebiotic test), incubated at 36 ± 1°C / 24 h; then the percentage of post–treatment cell survival was calculated. Showing that, formulation 1 presented higher barrier protection with average counts: 7.31 log CFU·g-1 lactobacilli and 7.75 log CFU·g-1 (Saccharomyces boulardii) / 4 h (SGF), reaching 6.78 log CFU·g-1 in the four formulations (SIF) with a higher average survival rate 79.79% and 85.06% SGF and SIF, in vitro. On the other hand, the prebiotic test maintained average counts of 9.40 log CFU·g-1 (Lactobacillus spp.) and 6.99 log CFU·g-1 (S. boulardii) / 24 h. The protection exerted by the microspheres under simulated gastrointestinal and prebiotic conditions at therapeutic levels (≥ 106 CFU·mL-1) was demonstrated.","PeriodicalId":54478,"journal":{"name":"Revista Cientifica-Facultad De Ciencias Veterinarias","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135854510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of myo–inositol supplementation on feed physicochemical structure and viral load of dry cat food contaminated with SARS–CoV–2 by simulating sneezing","authors":"Serol Korkmaz, Ayşe Parmaksız, Burcu Irem Omurtag–Korkmaz, Ahmet Sait","doi":"10.52973/rcfcv-e33282","DOIUrl":"https://doi.org/10.52973/rcfcv-e33282","url":null,"abstract":"The study was carried to investigate the effect of myo–inositol supplementation on feed physicochemical structure and viral load of dry cat food contaminated with inactive SARS–CoV–2 by simulating sneezing. The most natural infection of severe acute respiratory syndrome coronavirus 2 (SARS–CoV–2) in animals is related to close contact with their owners with COVID–19 which is handling, taking care and feeding them. SARS–CoV–2 can survive on food, fomites and surfaces for extended periods related to environmental conditions. Many natural feed additives and supplements have been a candidate in recent antiviral treatment strategies against COVID–19. In this study, myo–inositol which is permitted in animal nutrition was used at different concentrations (0, 12.5, 25 and 50 mg·100 g-1 cat food) and conditions (22°C at room temperature and 4°C in the refrigerator) to investigate its effects on feed physicochemical structure and viral load of dry cat food contaminated with inactive SARS–CoV–2 by simulating sneezing. For the interactions between myo–inositol, feed structure and viral load, dry matter, moisture, water absorption index (WAI), water solubility index (WSI), pH and virus gene copy (GC) by RT–qPCR were measured. As only storage temperature affected both WAI and WSI as expected, myo–inostol supplementation dose–dependently decreased gene copy in dry cat food (IC50:366.4–581.5 mg·100 g-1 cat food) at 22°C storage temperature. Virus GC did not correlate with the dry matter, moisture content, pH and WAI after the 30 min contact time (except WSI). In conclusion, myo–inositol as a feed additive might have the potential to control serious viral infections such as COVID–19 for human–animal interactions in a One–Health context.","PeriodicalId":54478,"journal":{"name":"Revista Cientifica-Facultad De Ciencias Veterinarias","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135855531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Análisis de la gestión técnico–económica de ganaderías ovinas de razas autóctonas de las Islas Baleares (España)","authors":"Yolanda Mena, Manuel Delgado–Pertiñez","doi":"10.52973/rcfcv-e33299","DOIUrl":"https://doi.org/10.52973/rcfcv-e33299","url":null,"abstract":"Las Islas Baleares, España, poseen una importante tradición ganadera basada en el uso de razas autóctonas y en el pastoreo. Sin embargo, la intensificación de la producción primaria, la competencia por el territorio generado por el turismo y su baja rentabilidad, hacen que estos sistemas estén a punto de desaparecer, con negativas consecuencias ambientales y sociales. Para poder conservar estos sistemas, es necesario profundizar en el conocimiento del manejo, lo que permitirá mejorar su gestión técnica y económica y establecer estrategias que optimicen su rentabilidad, pero conservando sus beneficios socio–ambientales. Se seleccionaron 10 unidades productivas ovinas de dos razas autóctonas de Mallorca (Mallorquina y Roja Mallorquina) que fueron monitorizadas mensualmente durante 2015, con el objetivo de evaluar la gestión mediante la elaboración de indicadores técnico–económicos. Posteriormente, se realizó una evaluación de las debilidades del manejo, para detectar aspectos clave que ayuden a mejorar la rentabilidad de la actividad. Se observó que estas unidades productivas están poco especializadas y presentan diversos modelos productivos (profesional, recreativo, turístico, agrícola). Todas ellas están orientadas a la producción de corderos, menos una que es mixta, produciendo tres tipos diferentes de corderos según su peso (lechal, recental medio y grande). La autonomía alimentaria de las unidades productivas es alta, sin embargo, poseen poca organización reproductiva y los costes de producción no se cubren por el precio del cordero. Así mismo, solo el 30 % de las unidades productivas genera beneficio empresarial. Como estrategias de mejora se considera importante la mejora de la producción de pastos; la mejora de la planificación reproductiva y de la genética de las razas; el producir corderos de buena conformación de la canal o el crear una marca de calidad que informe al consumidor de los beneficios ambientales de estos sistemas y de la calidad nutritiva–funcional que tienen estos corderos criados en pastoreo.","PeriodicalId":54478,"journal":{"name":"Revista Cientifica-Facultad De Ciencias Veterinarias","volume":"298 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135197575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in hematological status depending on reproductive stage of ewes naturally infected with internal parasites in North–Eastern Algeria","authors":"Ahmed Hadef, Souad Righi, Abdelbasset Ghouar","doi":"10.52973/rcfcv-e33308","DOIUrl":"https://doi.org/10.52973/rcfcv-e33308","url":null,"abstract":"In Algeria, sheep farming is still largely traditional, and ewes are subject to various pathologies, particularly parasitic diseases leading to reproductive failure and production losses. To assess the influence of internal parasites (helminths and protozoa) and their interaction with the different phases of reproduction and season on hematological parameters, fecal and blood samples (n = 89) were taken from ewes during the period from the end of gestation to 3 months after parturition, two weeks apart. Animals were reared under a traditional pasture–based farming system in a Region of Northeastern Algeria characterized by a Mediterranean climate. Parasitic coproscopy results showed the consistent presence of digestive strongyles, Strongyloides, Trichuris ovis, Moniezia spp., Fasciola hepatica, and Coccidia. Hematological parameters, mainly hematocrit (HCT), hemoglobin (HGB), and erythrocytes (RBC), showed lower levels in anemic ewes throughout the study period. These hematological effects became significantly prevalent in 91% of the animals (P<0.05) during the third month postpartum (p.p.). The use of a mixed–effects model confirmed the significant hematological effect (P<0.05) of internal parasitism, which was amplified significantly by its interaction with the reproduction stage for HCT and RBC (P<0.01). Monitoring of blood biological constants in ewes during the critical reproductive stages seems to provide valuable data to improve their welfare and resistance to potential internal parasites.","PeriodicalId":54478,"journal":{"name":"Revista Cientifica-Facultad De Ciencias Veterinarias","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135194060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological characterization of hard palate in the Tabby cats","authors":"Ayşe Gizem Ermiş, Mehmet Can","doi":"10.52973/rcfcv-e33285","DOIUrl":"https://doi.org/10.52973/rcfcv-e33285","url":null,"abstract":"The aim of this study was to carry out the morphological characterization of the Tabby cat’s hard palate by light and scanning electron microscopy (SEM), in addition to gross and morphometric analysis. A total of 20 Tabby cat heads used. The materials was provided from the Balıkesir Metropolitan Municipality Street Animals Temporary Nursing Home and Rehabilitation Center, Balıkesir, Turkey. Adult, regardless of gender difference, died from variety of reasons and 20 Tabby cat cadavers which weigh approximately 3–4 kg were brought to the laboratory in accordance with the procedure. Hard palates were subjected to gross examination, morphometric analysis, light and SEM.The Tabby cats hard palate was split up rostral and caudal parts and incisive papilla (papilla incisive), rugae palatine and with a gutter between them, the presence of palatine raphe and secondary rugae palatine were observed. The rostral and caudal parts of the hard palate were determined to have a smooth appearance with SEM 540X magnification and the presence of epithelial desquamations was found out. It was determined that the rostral and caudal part of the Tabby cat’s hard palate had a honeycomb appearance of the microplicae of the 3000X magnification hard palate epithelium with SEM. Round islets were detected with the SEM at the magnification of 600X of the incisive papilla, the 270X of the rostral–caudal parts and the 44X of the gutter between the palatine rugae. These adaptations of the Tabby cat’s hard palate might increase its efficiency during ingestion and mastication of hard food and in directing the food backward. The study shows the detailed anatomical description of the hard palate of the Tabby cat with light and SEM.","PeriodicalId":54478,"journal":{"name":"Revista Cientifica-Facultad De Ciencias Veterinarias","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135536206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma profile of Pregnancy Associated Glycoproteins during Postpartum period in Konya Merino ewes lambing single and twin","authors":"Eyyup Hakan Ucar, Mehmet Kose, Mesut Kirbas, Faruk Bozkaya, Mehmet Osman Atli","doi":"10.52973/rcfcv-e33298","DOIUrl":"https://doi.org/10.52973/rcfcv-e33298","url":null,"abstract":"This study investigated the plasma profile of Pregnancy Associated Glycoproteins (PAGs) after lambing in Konya Merino ewes using specific cattle pregnancy test kit. A total of 16 Konya Merino ewes were used as a material. Four groups were set up, ewes birthing a male lamb (SM group, n=4), a female lamb (SF group, n=4), twin male lambs (TM group, n=4) or twin female lambs (TF group, n=4). Blood plasma samples were taken on days 0 (lambing day), 3, 7, 10, 14 and then weekly until day 35. All samples were analysed using a bovine pregnancy test kit to detect of PAGs level. It was determined that there was a strong negative correlation between PAGs levels in peripheral blood and days after lambing (r2=0.969; P<0.01). However, no relationship was found between PAGs level and lamb gender or birth type. In conclusion, plasma PAGs level decreases rapidly in Konya Merino ewes regardless of lamb gender and birth type. Moreover, the results showed that the plasma profile of PAGs after lambing in Konya Merino ewes can be monitored with a bovine commercial ELISA–based pregnancy test kit, and the test results can be used in decisions and assessments based on the levels of PAG molecules.","PeriodicalId":54478,"journal":{"name":"Revista Cientifica-Facultad De Ciencias Veterinarias","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134957678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of changes in Hematological parameters and levels of Oxidative stress factors in castrated Cats","authors":"Eren Polat, Tutku Can Acisu, Mustafa Sönmez, Emine Ünsaldi, Önder Otlu, Murat Tanrisever, Aslıhan Çakır, Yasin Baykalir","doi":"10.52973/rcfcv-e33290","DOIUrl":"https://doi.org/10.52973/rcfcv-e33290","url":null,"abstract":"This study was carried out to determine the effect of castration procedure on hemogram and leukogram values and the levels of oxidative stress factors in serum tissue in cats. Preoperative and postoperative 10th day hemogram/leukogram values and total oxidant/antioxidant capacities of 19 castrated cats were evaluated in the study. When the hemogram and leukogram values were examined, it was determined that there was a statistically significant increase in the amount of eosinophils (P<0.05). Although the increase in white blood cells (WBC), neutrophil and lymphocyte levels and the decrease in red blood cells (RBC) and hematocrit (HCT) levels were remarkable, it was not found to be statistically significant (P>0.05). When the levels of oxidative stress factors in the serum tissues of the cats were examined, it was determined that there was a decrease in the total antioxidant status (TAS) and an increase in the total oxidant status (TOS), but the difference in the results was not statistically significant (P>0.05). In conclusion, in this study, it was determined that the castration procedure in cats did not cause a significant difference in hemogram and leukogram parameters and the level of oxidative stress factors in serum tissue, except for the amount of eosinophils.","PeriodicalId":54478,"journal":{"name":"Revista Cientifica-Facultad De Ciencias Veterinarias","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135864620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Brucella canis infection in Pit Bull breed dogs in Turkey","authors":"Volkan Özavci, Hafize Tuğba Yüksel Dolgun, Yiğit Seferoğlu, Şükrü Kirkan","doi":"10.52973/rcfcv-e33304","DOIUrl":"https://doi.org/10.52973/rcfcv-e33304","url":null,"abstract":"Brucella canis infection is an often neglected but important zoonotic disease. This study aims to determine its seroprevalence in Pit Bull dogs from the Western Region of the Turkish Anatolian Peninsula. In the Province of Manisa, 2 mL blood samples were taken from the antebrachial region of 35 Pit Bull dogs using sterile K2EDTA (3.6 mg) blood tubes, and the samples were analyzed using both the mercaptoethanol (ME) microagglutination test and B. canis–specific PCR techniques. Of the 35 dogs tested by 2–ME RSAT, 13 (37.14%) tested positive and 22 (63%) tested negative. Of the 13 dogs that tested positive for 2–ME RSAT, 8 (22.85%) were female, and 5 (14.28%) were male. Subsequent PCR analysis of all samples revealed that 7 (20%; 7/35) of the samples that tested positive for 2–ME RSAT were actually B. canis–specific PCR positive. These findings suggest that B. canis is present in Pit Bull dogs, although they provide a general idea of the disease's prevalence of the disease in the region. Multicentre studies with larger numbers of cases in different groups of Pit Bulls, such as healthy, patient and risk groups, are needed to provide comprehensive evidence.","PeriodicalId":54478,"journal":{"name":"Revista Cientifica-Facultad De Ciencias Veterinarias","volume":"129 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135925959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of tumor necrosis Factor Alpha, Interferon Gamma, Procalcitonin and Neopterin levels in Brucella seropositive cattle","authors":"Nevin Tuzcu, Mehmet Tuzcu, Gokhan Akcakavak","doi":"10.52973/rcfcv-e33271","DOIUrl":"https://doi.org/10.52973/rcfcv-e33271","url":null,"abstract":"Brucellosis is a zoonotic disease that affects a large number of people and animals, causing physical disability, workforce loss and significant economic losses in the livestock industry. In the current study, it was aimed to determine and compare the levels of tumor necrosis factor alpha (TNF–α), interferon gamma (IFN–γ), Procalcitonin (PCT) and Neopterin in the blood serums of cattle with brucellosis and vaccinated against brucellosis. The materials of this study consisted of a total 48 blood serums belonging to three basic groups, each consisting of 16 animals. Disease group (1st group) were divided into two subgrups each consisting of 8 animals that 21st day after abortion and seropositive 7 months pregnant, the vaccinated (2nd group) and the control (3rd group) groups were divided into two subgroups, each consisting of 8 animals that gave birth 21 days ago and 7 months pregnant. IFN–γ and PCT levels were determined by sandwich enzyme immunoassay, TNF–α and Neopterin levels were determined using competitive inhibition enzyme immunoassay method by using ELISA device. In this study, TNF–α, PCT and Neopterin levels measured in the blood serums of the Brucella seropositive (1st), conjunctival Brucella abortus S19 vaccine administered (2nd) and unvaccinated Brucella seronegative control groups were compared and no significant difference could be determined between the subgroups of the groups (P>0.05). There were a significant differences between 1st, 2nd, and 3rd groups (P<0.05). IFN–γ levels determined in the blood serums of 1st, 2nd and 3rd groups were compared and nosignificant differences were found between the subgroups of 2nd and 3rd groups (P>0.05), but there were a significant differences between the subgroups of the 1st group (P<0.05). Similarly, a significant differences were determined between 1st, 2nd and 3rd groups in terms of IFN–γ levels (P<0.05). As a result, it was thought that detecting very high serum TNF–α, IFN–γ, neopterin levels in cattle with brucellosis would be helpful in the diagnosis and follow–up of brucellosis. However, it was concluded that there is a need for controlled studies comparing more herds with brucellosis to determine whether the relevant cytokines can be used in the diagnosis of brucellosis.","PeriodicalId":54478,"journal":{"name":"Revista Cientifica-Facultad De Ciencias Veterinarias","volume":"113 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136383998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryptococcus neoformans en la cavidad nasal de un gato en Costa Rica. Reporte de caso","authors":"Andrea Urbina-Villalobos, Isabel Hagnauer, N. Méndez, Natalia Guzmán-Gómez, Alejandra Calderón-Hernández, J. A. Morales","doi":"10.15359/rcv.36-2.3","DOIUrl":"https://doi.org/10.15359/rcv.36-2.3","url":null,"abstract":"La criptococosis felina es causada por las levaduras encapsuladas de los complejos Cryptococcus neoformans y Cryptococcus gattii. Esta es la micosis sistémica más frecuente en felinos, los cuales pueden ser asintomáticos y presentar signos respiratorios o neurológicos. No han sido reportados casos en Costa Rica. Se recibió un paciente felino, macho, sin raza definida, pelo corto, de 4 años de edad, con historia de 6 meses de presentar estornudos y secreción nasal incolora, unilateral, refractaria a los tratamientos administrados. Se le realizó un lavado y una biopsia de mucosa nasal para análisis citológico y micológico. En la citología, se observó un proceso inflamatorio piogranulomatoso y gran cantidad de levaduras encapsuladas. Estas se observaron también en el examen microscópico directo con KOH, tinta china y frotis teñido con Giemsa. En cultivo, se aisló Cryptococcus sp. y se confirmó C. neoformans, mediante API 20 C AUX (Biomérieux®). Se administró tratamiento durante 9 meses y el gato evolucionó favorablemente. Se debe considerar la criptococosis en el diagnóstico diferencial de pacientes felinos con descargas nasales unilaterales incoloras y crónicas. La confirmación se debe realizar mediante pruebas de laboratorio para instaurar el tratamiento pertinente.","PeriodicalId":54478,"journal":{"name":"Revista Cientifica-Facultad De Ciencias Veterinarias","volume":"16 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2018-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81753041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}