Labmedicine最新文献_第7页

Propofol Attenuates Human Proximal Renal Tubular Epithelial Cell Injury Induced by Anoxia-Reoxygenation 异丙酚减轻缺氧-复氧诱导的人近端肾小管上皮细胞损伤

4区医学

Labmedicine Pub Date : 2015-09-23 DOI: 10.1309/YVAD9JBUFGVGAAVP

Ya-ni Feng, T. Bai, Hong Ma, Jun-ke Wang

{"title":"Propofol Attenuates Human Proximal Renal Tubular Epithelial Cell Injury Induced by Anoxia-Reoxygenation","authors":"Ya-ni Feng, T. Bai, Hong Ma, Jun-ke Wang","doi":"10.1309/YVAD9JBUFGVGAAVP","DOIUrl":"https://doi.org/10.1309/YVAD9JBUFGVGAAVP","url":null,"abstract":"Background We undertook a study to determine whether propofol pretreatment may protect human proximal renal tubular epithelial (HK-2) cells against anoxia-reoxygenation injury and the role of p38 mitogen-activited protein kinase (p38MAPK).\u0000\u0000Methods HK-2 cells were randomly divided into 5 groups: a control group (Con); a group treated for 1 hour, cultured with with 300 μM CoCl2 normal medium for 24 hours, then stimulated with no serum media (group CoCl2); a group where the HK-2 cells were pretreated with 5μg/mL−1 propofol for 1 hour, then were treated as group (group Pro); a group where we pretreated CoCl2 the HK-2 cells with 10% intralipid 90 μL for 1 (group Int); hour, then treated as group CoCl2 and a group where we pretreated the HK-2 cells with propofol for 1 hour, 10 μmol/L−1SB203580 were (inhibitor of p38MAPK), and 300 μM CoCl2 added at the same time, then cultured with normal medium for 24 hours (group SB). Using MTT method and flow cytometry to detect the proliferation and apoptosis of HK-2 cells, the reverse transcriptase-polymerase chain reaction method was used to investigate the regulation of Bcl-2 and caspase-3 mRNA; the expression of p38MAPK was measured by Western blot.\u0000\u0000Results After pretreated with 5 μg/mL−1 propofol, HK-2 cell proliferation increased and apoptosis decreased ( P <0.05 or 0.01). Intralipid did not effect HK-2 cells, the expression of Bcl-2 gene was upregulated, and caspase-3 gene was down-regulated. Meanwhile, the expression of p38MAPK was upregulated after propofol pretreament. These regulations can be reversed by SB203580 ( P <0.05 or 0.01) with no difference between group Int and group CoCl2.\u0000\u0000Conclusion Pretreatment with 5μg/mL−1 propofol protected HK-2 cells against anoxia-reoxygenation injury at clinically relevant concentrations by regulating the expression of apoptosis related genes. P38MAPK plays an important role in propofol protective effect.","PeriodicalId":54328,"journal":{"name":"Labmedicine","volume":"104 1","pages":"356-360"},"PeriodicalIF":0.0,"publicationDate":"2015-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74286253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Head and Neck Pain, Syncopal Episode, Abnormal Cerebrospinal Fluid, and Hyponatremia in a Middle-Aged Woman 头颈疼痛、晕厥发作、脑脊液异常和低钠血症一例中年妇女

4区医学

Labmedicine Pub Date : 2015-09-23 DOI: 10.1309/1L5W3YXVTT5L98W9

M. Pérez, L. Bush, S. Rodriguez

{"title":"Head and Neck Pain, Syncopal Episode, Abnormal Cerebrospinal Fluid, and Hyponatremia in a Middle-Aged Woman","authors":"M. Pérez, L. Bush, S. Rodriguez","doi":"10.1309/1L5W3YXVTT5L98W9","DOIUrl":"https://doi.org/10.1309/1L5W3YXVTT5L98W9","url":null,"abstract":"1. Important clinical information includes neck pain, the absence of fever or focal neurologic signs, and syncopal episode. The most striking laboratory findings include the abnormal chemical and cytologic analysis of the cerebrospinal fluid (CSF) sample (hypoglycorrhachia, proteinorrhachia, and pleocytosis) and mild hyperglycemia. 2. Hypoglycorrhachia: The CSF is normally a clear, colorless, and virtually acellular fluid that is actively produced by the choroid plexus and ependymal cells lining the central nervous system (CNS) ventricles and subarachnoidal space through a process of ultrafiltration of plasma. Glucose levels in the CSF are 60% to 70% those of the serum; therefore, comparison between both values is paramount in interpreting abnormal results. Increased CSF glucose (hyperglycorrhachia) may be seen in the context of hyperglycemia or be due to a traumatic tap. Conversely, decreased CSF glucose (hypoglycorrhachia) is commonly found in untreated bacterial, fungal, tuberculous, or amoebic meningitis, and rarely in some viral CNS infections (particulary those caused by mumps and lymphocytic choriomeningitis viruses). Other causes of hypoglycorrhachia include CNS sarcoidosis and neoplasms (primary or metastatic). Proteinorrhachia: The normal CSF protein level is about 1% of the serum protein level. Increased levels are seen with most infections involving the meninges and the CNS, but are Clinical History","PeriodicalId":54328,"journal":{"name":"Labmedicine","volume":"15 1","pages":"337-340"},"PeriodicalIF":0.0,"publicationDate":"2015-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87632522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of the Glycoprotein IIb/IIIa Inhibitor Tirofiban on Concentrations of Whole Blood Choline in Acute Coronary Syndromes 糖蛋白IIb/IIIa抑制剂替罗非班对急性冠脉综合征患者全血胆碱浓度的影响

4区医学

Labmedicine Pub Date : 2015-09-23 DOI: 10.1309/0TQB1HAUX9JFU1B0

C. Storm, O. Danne, C. Lueders, U. Frei, M. Möckel

引用次数: 0

Insidious Onset of Visual Disturbances in a Healthy 56-Year-Old Man 一名56岁健康男性潜伏性视力障碍

4区医学

Labmedicine Pub Date : 2015-09-23 DOI: 10.1309/QDNQCF9JP22WK96E

M. Sealfon, Seth Franklin

引用次数: 2

Managing Workforce Health and Wellness 管理劳动力健康和福利