Neobiota最新文献

{"title":"Multi-locus environmental DNA markers and taxon-specific primers enhanced detection rate of invasive tilapia","authors":"M. Fang, Yili Qi, Hui Wang, Chenhong Li","doi":"10.3897/neobiota.101.143614","DOIUrl":"https://doi.org/10.3897/neobiota.101.143614","url":null,"abstract":"Tilapia are native to Africa. Due to introductions for aquaculture and the ornamental fish trade, several species of tilapia have now spread across five continents, making them some of the most ecologically harmful invasive species. During the early stages of their escape, tilapia are often difficult to detect using traditional fish survey methods. Using environmental DNA (eDNA) methods to monitor tilapia invasion is a promising supplementary approach, but currently no eDNA markers or suitable primers have been developed specifically for tilapia. In this study, we designed four pairs of tilapia-specific primers for use in multiplex PCR coupled with high-throughput sequencing, enabling the simultaneous detection of multiple tilapia species in a single reaction. We found that the taxon-specific primers (Tila-1–4) exhibited higher detection sensitivity compared to the universal primer (tele02), reaching 5.33 × 10 -7 ng/µL. Furthermore, multi-locus amplification reached a 95% limit of detection (LOD) at 2.66 × 10 −6 ng/µL, which was significantly better than 2.3 × 10 -3 ng/µL for single-locus amplification. Finally, we collected water samples from nine rivers across four drainages in China to test the method that we developed. The results indicated that tilapia have invaded river systems such as the Yangtze River and the Pearl River. Specifically, Oreochromis niloticus and Coptodon zillii were the dominant invasive species detected, with some locations also showing the presence of O. aureus and Sarotherodon galilaeus . This method provides an effective tool for early monitoring of tilapia invasion, which may inspire the development of similar strategies for monitoring other invasive species.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":"101 1","pages":"185-202"},"PeriodicalIF":0.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://neobiota.pensoft.net/article/143614/download/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147917758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of microbiomes in animal invasions: a scoping review","authors":"Claudia Romeo, Elsa Brenner, Lucas A. Wauters, Antton Alberdi","doi":"10.3897/neobiota.98.145939","DOIUrl":"https://doi.org/10.3897/neobiota.98.145939","url":null,"abstract":"Despite increasing evidence for the contribution of microbiomes to host fitness and evolution, their role in the adaptation and successful establishment of invasive animal species remains underexplored. Animal microbiomes can mediate key host phenotypic traits such as energy metabolism, detoxification and disease resistance. Therefore, harbouring a highly functional microbiome may be advantageous in the context of invasion, where small host populations must rapidly adapt to new environmental conditions. We conducted a scoping review of studies focusing on microbiomes and animal invasions to explore the extent and nature of research efforts on this topic and to identify general patterns that may help elucidate the relationship between host microbial communities and invasiveness. The analysis of 147 articles published between 2006 and 2024 showed a steady increase in the research output on the topic, in parallel with growing interest in biological invasions and technical and theoretical advances in microbiome research. However, the application of new analytical approaches that go beyond taxonomic characterisation remains limited and the research output is still heavily biased towards invasive invertebrates. Although most of the reviewed research was descriptive, a more detailed assessment of a subset of 43 studies using a comparative design revealed some recurring patterns. Host microbiomes in the introduction range tend to diverge from those in the native range, but invasive populations generally retain a core of microorganisms involved in key phenotypic traits such as disease resistance. Studies that have examined the microbiomes of invasive species along their invasion pathway highlight how stochastic events, propagule pressure and population mixing are relevant drivers of microbial community assembly during introductions. Comparisons of the microbiomes of invasive species and co-occurring, outcompeted native species often suggest that some of the observed phenotypic differences driving their interactions are microbiome-mediated. However, to date, only a handful of studies have been able to establish the mechanistic link between microbiomes and host invasiveness using an experimental design. While observational studies remain valuable at this early stage, we advocate for a wider use of novel technologies and experimental approaches to generate robust functional and mechanistic information that will strengthen their inferential value. As more system-specific studies become available, meta-analytical approaches may allow us to uncover broader eco-evolutionary patterns and ultimately elucidate the role of microbiomes in animal invasions.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":"98 1","pages":"335-360"},"PeriodicalIF":0.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://neobiota.pensoft.net/article/145939/download/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147890046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution and genetic diversity of the invasive pest Halyomorpha halys (Hemiptera, Pentatomidae) in Belgium","authors":"Olivier Hendrik Berteloot, Alexandre Kuhn, Gertie Peusens, Tim Beliën, Louis Hautier, Thomas Van Leeuwen, Patrick De Clercq","doi":"10.3897/neobiota.90.113421","DOIUrl":"https://doi.org/10.3897/neobiota.90.113421","url":null,"abstract":"\u0000The brown marmorated stink bug, Halyomorpha halys, native to East Asia, is an invasive pest of economic importance. It has invaded North America and many European countries and is further expanding its range. In Belgium, it was first observed in 2011. Halyomorpha halys is known to cause severe damage in commercial fruit orchards and vegetable crops. A dramatic and unmitigated expansion of H. halys in its adventive range could lead to significant economic implications for agricultural production. In this study, occurrence data of H. halys since its first observation in Belgium was analysed together with molecular information to map the populations and evaluate the genetic diversity of this pest. The genetic diversity of H. halys in Belgium was compared to data from other invaded and native countries reported in previous studies to identify possible invasion routes. The analysis of 1176-bp of mitochondrial DNA cytochrome c oxidase I and II genes (COI and COII) led to the discovery of two novel COI-COII haplotype combinations currently unique to Belgium. The invasion of H. halys in Belgium is likely the result of multiple and ongoing introductions from its native region and from already invaded countries in Europe, particularly Italy. The expansion of the brown marmorated stink bug populations in Belgium is recent and ongoing. Presently, it appears to thrive best in northern Belgium.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":"25 1","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139500120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root hemiparasites suppress invasive alien clonal plants: evidence from a cultivation experiment","authors":"Tamara Těšitelová, Kateřina Knotková, Adam Knotek, Hana Cempírková, Jakub Těšitel","doi":"10.3897/neobiota.90.113069","DOIUrl":"https://doi.org/10.3897/neobiota.90.113069","url":null,"abstract":"\u0000Alien invasive plants threaten biodiversity by rapid spread and competitive exclusion of native plant species. Especially, tall clonal invasives can rapidly attain strong dominance in vegetation. Root-hemiparasitic plants are known to suppress the growth of clonal plants by the uptake of resources from their below-ground organs and reduce their abundance. However, root-hemiparasites’ ability to interact with alien clonal plants has not yet been tested.\u0000We explored the interactions between native root-hemiparasitic species, Melampyrum arvense and Rhinanthus alectorolophus and invasive aliens, Solidago gigantea and Symphyotrichum lanceolatum. We investigated the haustorial connections and conducted a pot experiment. We used seeds from wild hemiparasite populations and those cultivated in monostands of the invasive plants to identify a possible selection of lineages with increased compatibility with these alien hosts. The hemiparasitic species significantly suppressed the growth of the invasive plants. Melampyrum inflicted the most substantial growth reduction on Solidago (78%), followed by Rhinanthus (49%). Both hemiparasitic species reduced Symphyotrichum biomass by one-third. Additionally, Melampyrum reduced the shoot density of both host species. We also observed some transgenerational effects possibly facilitating the growth of hemiparasites sourced from subpopulations experienced with the host.\u0000Native root hemiparasites can effectively decrease alien clonal plants’ biomass production and shoot density. The outcomes of these interactions are species-specific and may be associated with the level of clonal integration of the hosts. The putative selection of lineages with higher performance when attached to the invasive novel hosts may increase hemiparasites’ efficiency in future biocontrol applications.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":"147 1","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139470200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the invasion potential of five common exotic vine species in temperate Australian rainforests","authors":"Adam Bernich, Kris French, Michael Bedward","doi":"10.3897/neobiota.90.110659","DOIUrl":"https://doi.org/10.3897/neobiota.90.110659","url":null,"abstract":"\u0000To compare the capacity of native and exotic vine species established under a rainforest canopy, a comparison of growth rates and resource allocation was made amongst five exotic vine species that are serious and common invaders and two common native vine species under two light conditions reflective of edge and interior canopy conditions. All species experienced heavy reductions in growth parameters in the low-light treatment, but three exotic species showed stronger growth under the low light. All exotic species had higher plasticity in leaf morphology showing a significant increase in SLA under low light. Native vines may have a lower capacity to change leaf morphology in shade, as a result of local adaptation to edge habitats. Higher SLA under both low and high light conditions suggests that exotic vines species are able to exploit a range of forest conditions better than the native species. Three species, Anredera cordifolia, Araujia sericifera and Cardiospermum grandiflorum, appear particularly capable of invading rainforest interiors. Individuals produced few leaves, focusing resources on roots and stems suggesting a response to reach the canopy quickly. With their long-distance seed dispersal, plasticity in leaf SLA and high RGR, these species appear most likely to invade undisturbed rainforest.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":"151 1","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139410727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Worldwide distribution and phylogeography of the agave weevil Scyphophorus acupunctatus (Coleoptera, Dryophthoridae): the rise of an overlooked invasion","authors":"Andrea Viviano, Arturo Cocco, Paolo Colangelo, Giuseppe Marco Delitala, Roberto Antonio Pantaleoni, Laura Loru","doi":"10.3897/neobiota.90.101797","DOIUrl":"https://doi.org/10.3897/neobiota.90.101797","url":null,"abstract":"\u0000Global plant trade represents one of the main pathways of introduction for invertebrates, including insects, throughout the world. Non-native insects include some of the most important pests affecting cultivated and ornamental plants worldwide. Defining the origins and updating the distribution of non-native invasive species is pivotal to develop effective strategies to limit their spread. The agave weevil, Scyphophorus acupunctatus (Coleoptera, Dryophthoridae), is a curculionid beetle native to Central and North America, although it also occurs in Eurasia, Africa, Oceania and South America as a non-native species. Despite being widespread, the extent of occurrence and origins of European populations of the agave weevil have been overlooked. In the present study, the current and potential worldwide distribution of S. acupunctatus was assessed and an analysis of its genetic diversity in the native and non-native ranges was performed. By analysing occurrences from local phytosanitary bulletins and citizen-science platforms, the agave weevil was confirmed to be widely distributed and to occur on all continents, except Antarctica. Additionally, there is potential for expansion throughout the world, as estimated by species distribution models. Nucleotide and haplotype diversity of the COXI mitochondrial gene (about 650 bp) was lower in the non-native (n = 39 samples) than native populations (n = 26 samples). The majority of introduced individuals belonged to the same haplotype, suggesting that most introductions in Europe might have occurred from a small geographical area in Central America. Constant transboundary monitoring and national laws must be considered to reduce the spread of the agave weevil, given that a bridgehead effect may occur from non-native populations to new suitable areas.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":"23 1","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139410380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asymmetrical insect invasions between three world regions","authors":"Rylee Isitt, Andrew M. Liebhold, Rebecca M. Turner, Andrea Battisti, Cleo Bertelsmeier, Rachael Blake, Eckehard G. Brockerhoff, Stephen B. Heard, Paal Krokene, Bjørn Økland, Helen F. Nahrung, Davide Rassati, Alain Roques, Takehiko Yamanaka, Deepa S. Pureswaran","doi":"10.3897/neobiota.90.110942","DOIUrl":"https://doi.org/10.3897/neobiota.90.110942","url":null,"abstract":"\u0000The geographical exchange of non-native species can be highly asymmetrical, with some world regions donating or receiving more species than others. Several hypotheses have been proposed to explain such asymmetries, including differences in propagule pressure, source species (invader) pools, environmental features in recipient regions, or biological traits of invaders. We quantified spatiotemporal patterns in the exchange of non-native insects between Europe, North America, and Australasia, and then tested possible explanations for these patterns based on regional trade (import values) and model estimates of invader pool sizes. Europe was the dominant donor of non-native insect species between the three regions, with most of this asymmetry arising prior to 1950. This could not be explained by differences in import values (1827–2014), nor were there substantial differences in the sizes of modelled invader pools. Based on additional evidence from literature, we propose that patterns of historical plant introductions may explain these asymmetries, but this possibility requires further study.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":"24 1","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139397043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Limitations of invasive snake control tools in the context of a new invasion on an island with abundant prey","authors":"Shane R. Siers, Melia G. Nafus, Jeried E. Calaor, Rachel M. Volsteadt, Matthew S. Grassi, Megan Volsteadt, Aaron F. Collins, Patrick D. Barnhart, Logan T. Huse, Amy A. Yackel Adams, Diane L. Vice","doi":"10.3897/neobiota.90.103041","DOIUrl":"https://doi.org/10.3897/neobiota.90.103041","url":null,"abstract":"\u0000In October 2020, a new population of invasive brown treesnakes (Boiga irregularis) was discovered on the 33-ha Cocos Island, 2.5 km off the south coast of Guam. Cocos Island is a unique conservation resource, providing refuge for many lizards and birds, including endangered species, which were extirpated from mainland Guam by invasive predators including brown treesnakes. We sought to evaluate the usefulness of toxic baiting with acetaminophen-treated carrion baits and cage trapping, common tools for the control of brown treesnakes on mainland Guam, as potential eradication tools on Cocos Island. We evaluated multiple bait types and bait presentations: on the ground, suspended in the canopy emulating aerial bait applications and in four plastic-tube bait station configurations intended to exclude non-target species. We monitored all baits with time-lapse cameras. Despite improved exclusion of non-targets by bait station design, most baits were quickly removed by non-target species, particularly coconut crabs (Birgus latro) and Mariana monitors (Varanus tsukamotoi). Monitoring of 1,250 baits available for 2,427 bait nights resulted in no observations of brown treesnakes taking any bait. Subsequently, we tested two trap types commonly used on Guam and compared trapping success with live versus dead mouse lures. In 10,553 trap nights using live and dead mouse lures, we only captured one brown treesnake, in a trap with a live mouse lure. These baiting and trapping rates are so low as to be ineffectual for all practical purposes. Concurrent visual searching and hand capture of brown treesnakes during initial rapid response efforts demonstrates that these low baiting and trapping success rates are not a result of low snake density. We make a case for our assumption that the ineffectiveness of these tools on Cocos Island is due to the context of extremely high abundance of preferred live prey, primarily large geckos and birds. Our results have profound conservation ramifications, because any future island invasions by brown treesnakes are likely to occur within similarly prey-rich environments where these baiting and trapping methods might be similarly ineffective.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":"12 1","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139104222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochar application can mitigate the negative impacts of drought in invaded experimental grasslands as shown by a functional traits approach","authors":"Hamada E. Ali, S. F. Bucher, M. Bernhardt‐Römermann, C. Römermann","doi":"10.3897/neobiota.89.109244","DOIUrl":"https://doi.org/10.3897/neobiota.89.109244","url":null,"abstract":"Climate, land-use, and invasive plants are among the important drivers of ecosystem functions through the changes in functional composition. In this study, we studied the effects of climate (drought), land-use (Biochar application), and the presence of invasive species on the productivity and performance of invaded experimental grasslands. We ran a greenhouse experiment under controlled conditions, in which we grew a combination of the three native species Silene gallica, Brassica nigra and Phalaris minor and the invasive species Avena fatua, being subjected to four different treatments: Biochar+drought, Biochar, drought, and control. We measured the productivity of native and invasive species as total biomass and root to shoot ratio (RSR) and the performance by measuring several plant functional traits (plant height, specific leaf area (SLA), leaf dry matter content (LDMC), leaf nitrogen content (Nmass), leaf carbon content (Cmass) and total chlorophyll (Chltotal) of all individuals occurring in each plot. The study showed that invasive species were more productive (higher total biomass and lower RSR) and performed better (taller plants, higher SLA, Nmass, Cmass and Chltotal and lower LDMC) than the native species under drought conditions as well as with Biochar application. Accordingly, in contrast to our expectations, the lower productivity and performance of native compared to invasive species under drought were not mitigated by Biochar application. These results provided a deeper understanding of the interplay between climate, land-use, and biological invasion, which is crucial for predicting the consequences of changes in functional composition on ecosystem functions and consequently restoration of grasslands.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":"1 3","pages":""},"PeriodicalIF":5.1,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138944400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cortaderia selloana, an example of aggressive invaders that affect human health, yet to be included in binding international invasive catalogues","authors":"Diego Liendo, Juan Antonio Campos, Alberto Gandarillas","doi":"10.3897/neobiota.89.110500","DOIUrl":"https://doi.org/10.3897/neobiota.89.110500","url":null,"abstract":"\u0000Invasive plant species can suppress local biodiversity, affect soil properties and modify the landscape. However, an additional concern of plant invasions that has been more disregarded is their impact on environmental human health. Here, we discuss the case of Cortaderia selloana (Schult. & Schult.f.) Asch. & Graebn, as an example of a worldwide invasive species with a strong environmental impact. We summarise the main facts regarding the C. selloana invasion, the recent clinical evidence of its impact on human health and the great potential expansion of the species in the context of climate change. C. selloana constitutes a clear example to boost demands from policy makers for urgent and efficient measures to control or eradicate invasive species, also in ruderal areas. This aggressive invader is still out of relevant binding international invasive species catalogues, including the European List of Invasive Alien Species of Union concern (Union list), and is still subjected to extensive trading in some European countries. Therefore, including C. selloana in the Union list becomes mandatory to impose full restrictions on keeping, importing, selling, breeding and cultivating the species.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":"29 1","pages":""},"PeriodicalIF":5.1,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138821026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}