Journal of Stem Cells最新文献_第2页

Influence of Violation of Regeneration in People over 35-40 Years Old on Decrease in Production of Sexual Hormones. 35-40岁人群违反再生对性激素分泌减少的影响。

Journal of Stem Cells Pub Date : 2016-01-01

A V Pechersky

{"title":"Influence of Violation of Regeneration in People over 35-40 Years Old on Decrease in Production of Sexual Hormones.","authors":"A V Pechersky","doi":"","DOIUrl":"","url":null,"abstract":"With age the quantity of pluripotent stem cells gradually decreases. It leads to violation of renewal of tissues, including tissues of endocrine organs, at people over 35-40 years old. Artificial formation of the chimeral individual may be used for recovery of the pool of pluripotent stem cells in patients older than 40-50 years old. Transfusions of mononuclear fraction of peripheral blood procured from young donors 18-23 years old with the same blood groups and sex as the recipient (patent of the Russian Federation № 2350340), allows people over 40 years old to reestablish the pool of pluripotent stem cells, the process of tissue renewal and the production of sex hormones, and can also be seen as a promising way to reduce biological age, while providing a significant prolongation of life and while maintaining a high quality of life. Transfusions of mononuclear fraction of peripheral blood are required to be carried out multiple times before the restoration of the numerical strength of the recipients' pool of pluripotent stem cells is complete. The effectiveness of regeneration reconstitution in individuals older than 40 years old upon receiving transfusions of allogeneic pluripotent stem cells depends on the difference in age between the recipient and the young donor. When there is a major difference in age between young donors and recipients over 40 years old, the proliferative potential of pluripotent stem cells of donors (their ability to maintain the number of own pool) is higher than the proliferative potential of the recipient. In response to the formation of colony-stimulating growth factors, this leads to the dominance of transfused and formed colonies in the bone marrow of the donor's pluripotent stem cells over the analogous cells of recipients when renewing all of their tissues. The latter occurs mainly due to the donors' stem cells.","PeriodicalId":53626,"journal":{"name":"Journal of Stem Cells","volume":"11 2","pages":"99-109"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34814735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Bone Marrow Processing Protocol for Therapeutic Applications via Culture and Characterization of Mesenchymal Stem Cells. 通过间充质干细胞的培养和表征评估骨髓处理方案的治疗应用。

Journal of Stem Cells Pub Date : 2016-01-01

Poonam Verma, Himanshu Bansal, Anupama Agrawal, Jerry Leon, I Birgitta Sundell, Prasad S Koka

{"title":"Evaluation of Bone Marrow Processing Protocol for Therapeutic Applications via Culture and Characterization of Mesenchymal Stem Cells.","authors":"Poonam Verma, Himanshu Bansal, Anupama Agrawal, Jerry Leon, I Birgitta Sundell, Prasad S Koka","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: Human mesenchymal stem cells from bone marrow (hMSCs) have broad therapeutic potential. These cells can be are readily isolated from bone marrow by their property to adhere to tissue culture treated culture wares. However, the proliferation rates and other properties of the cells gradually change during expansion. This study aims to validate the protocol of isolation and differentiation of hMSCs from bone marrow for therapeutic applications.Methods: Sixty ml of bone marrow was extracted from 5 patients and MSCs were isolated. These were characterized by Flow Cytometry, CFU assay and were differentiated into bone, fat cells and neurocytes.Results: The cells were having healthy morphology. These were positive for the markers CD105, CD90 and CD73 and negative for CD45, CD34 and HLA-DR. The cells could differentiate into fat, bone and neural cells.Conclusion: MSCs from the bone marrow were isolated and differentiated. These cells were morphologically healthy and passed CFU assay. The cells exhibited differentiation potential into bone, fat and neural tissue. These cells can be used in therapeutic applications.","PeriodicalId":53626,"journal":{"name":"Journal of Stem Cells","volume":"11 1","pages":"3-13"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34814816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mandibular Reconstruction in Ameloblastoma Using Allogeneic Cord Stem Cells and Alloplastic Graft Material - Case Report. 用同种异体脐带干细胞和同种异体移植材料重建下颌成釉细胞瘤1例报告。

Journal of Stem Cells Pub Date : 2016-01-01

K Manimaran, M Chandramohan, R Kannan, S Sankaranarayanan, V R Ravi, Rohini Sharma

引用次数: 0

Need for In Vivo Triggering of Homeostasis to Repair Irreversible Tissue Degeneration by Stem Cells through Innate and Invasive Regeneration Processes. 需要在体内触发稳态修复不可逆组织退化的干细胞通过先天和侵入性再生过程。

Journal of Stem Cells Pub Date : 2016-01-01

Jyothi S Navada, Prasad S Koka

{"title":"Need for In Vivo Triggering of Homeostasis to Repair Irreversible Tissue Degeneration by Stem Cells through Innate and Invasive Regeneration Processes.","authors":"Jyothi S Navada, Prasad S Koka","doi":"","DOIUrl":"","url":null,"abstract":"The regeneration patterns are innate, inherited and evolutionarily conserved mechanisms. In every individual there are certain cells and signaling networks which work together to proliferate the desired tissue lineages to replace the dead, lost and injured counter parts. This homeostasis mechanism keeps functioning of the organ system intact. There are some tissues such as skin, gut, blood, respiratory tract, uterine endometrium, testis must perpetually renew the majority of cells. As the aging advances the turnover potentials decreases under normal circumstances, some of these respond inefficiently to regenerative pressures (eg: brain and heart) while other respond quite well. Therefore creating an optimized micro environment using external means through non-invasive or invasive procedures preferably minimally by utilizing appropriately subjected stem cells/stem cell secretome to induce regeneration at the target sites where it does not take place spontaneously.Stem cell biology is one of the most attractive areas of biomedical research, as emerges for the execution of biotechnology towards the regenerative medicine continues to expand. The presumed potential of the stem cell populations with clonogenic capabilities are harnessed for the therapeutic applications. Advancements in the research technology and the idea of inducing innate regeneration by stem cell based approaches can generate the potential cure for many degenerative disorders, age related disabilities and accidental tissue damages.","PeriodicalId":53626,"journal":{"name":"Journal of Stem Cells","volume":"11 3","pages":"171-179"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34814609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regeneration and Cicatrization. 再生和愈合。

Journal of Stem Cells Pub Date : 2016-01-01

A V Pechersky, V I Pechersky, E S Shpilenya, A H Gaziev, V F Semiglazov

引用次数: 0

Therapy with Bone Marrow-Derived Autologous Adult Stem Cells in Quadriparesis due to Motor Neuron Disease. 骨髓来源的自体成体干细胞治疗运动神经元疾病所致四肢瘫。

Journal of Stem Cells Pub Date : 2016-01-01

Himanshu Bansal, Lipi Singh, Anupama Agrawal, Jerry Leon, I Birgitta Sundell, Prasad S Koka

{"title":"Therapy with Bone Marrow-Derived Autologous Adult Stem Cells in Quadriparesis due to Motor Neuron Disease.","authors":"Himanshu Bansal, Lipi Singh, Anupama Agrawal, Jerry Leon, I Birgitta Sundell, Prasad S Koka","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To report the safety and therapeutic effectiveness of application of concentrated bone marrow aspirate in three bedridden patients with weakness in both legs, and monitor potential improvement in neurological outcomes.Design: Case report. Intervention: Five infusions of 3x108 mononuclear cells were administrated with 12 week intervals. Bone marrow (240ML) were obtained from the posterior superior iliac spine and Bone marrow mononuclear cells were enriched by standard manual close method under aseptic condition.Results: During the follow-up study of one year after stem cell implantation, the conditions of all three patients were improved and were confirmed by physical assessment, muscle charting and Electromyography (EMG). One year after stem cell implantation patients who were bedridden before treatment could sit without support and walk with support up to 200 feet at a stretch.Conclusion: The local application of a cocktail of regenerative cell population found in an MNC fraction of bone marrow was safe and effective in improving quality of life and muscle strength in ALS patients. This case opens the need for further investigations on Autogenic stem cell transplant therapies for MND disease.","PeriodicalId":53626,"journal":{"name":"Journal of Stem Cells","volume":"11 1","pages":"15-23"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34814817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stage-Specific Regulation of Erythropoiesis and Its Implications in Ex-Vivo RBCs Generation. 红细胞生成的阶段特异性调控及其在体外红细胞生成中的意义。

Journal of Stem Cells Pub Date : 2016-01-01

Vimal Kishor Singh, Abhishek Saini, Manisha Kalsan, Neeraj Kumar, Ramesh Chandra

{"title":"Stage-Specific Regulation of Erythropoiesis and Its Implications in Ex-Vivo RBCs Generation.","authors":"Vimal Kishor Singh, Abhishek Saini, Manisha Kalsan, Neeraj Kumar, Ramesh Chandra","doi":"","DOIUrl":"","url":null,"abstract":"Ex vivo erythropoiesis methods are being developed for more than a decade now, and all the distinct types of stem cells (such as CD34+ HSCs, ESCs, IPSCs, and extensively proliferating erythropoietic progenitor cells) are defined to bear the potential for large scale RBC production shortly. The various regulating factors at different levels of RBCs production are being explored. Since most of the ex-vivo erythropoiesis protocols mimic the dogma followed by hematopoietic stem cells in vivo to give rise to mature RBCs which essentially deals with the intermediate stages of erythropoiesis such as burst forming unit-erythroid (BFU-E) and committed erythroid colony forming unit-erythroid (CFU-E). In vivo generation of erythroid progenitors (BFU-E/CFU-E) is essentially controlled by several factors including glucocorticoids, inflammation, and stress. Furthermore, regular production of functionally mature /transfusable units of RBCs is possible only through the coordinated regulation of terminal proliferation and differentiation of erythroid progenitors by external signals, such as erythropoietin, SCF, IL-3 and interaction to extracellular matrix protein(s) in a 3D culture system. We discuss these complex intracellular networks of coordinated factors and try to understand their molecular mechanism through gene regulation by transcription factors, and miRNAs that might be helpful in developing the optimal RBCs production protocols for commercial production.","PeriodicalId":53626,"journal":{"name":"Journal of Stem Cells","volume":"11 3","pages":"149-169"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34815204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial - Mesenchymal Stem Cells in Androgenic Alopecia: Hair Loss Regeneration. 编辑-雄激素性脱发的间充质干细胞:脱发再生。

Journal of Stem Cells Pub Date : 2016-01-01

Prasad S Koka

引用次数: 0

Immune System and Regeneration. 免疫系统和再生。

Journal of Stem Cells Pub Date : 2016-01-01

A V Pechersky, V I Pechersky, M V Aseev, A V Droblenkov, V F Semiglazov

{"title":"Immune System and Regeneration.","authors":"A V Pechersky, V I Pechersky, M V Aseev, A V Droblenkov, V F Semiglazov","doi":"","DOIUrl":"","url":null,"abstract":"The review article is devoted to a role of pluripotent stem cells and immune system in renewal of tissues (regeneration). Cell-precursors (progenitor cells) and differentiated cells can be divided a limited number of times and aren't capable of ensuring regeneration of tissues during the whole process of ontogenesis. The renewal of tissues during the whole long period is impossible without the participation of a specialized system which is responsible for regeneration. The given system is made up of pluripotent stem cells which are capable of differentiating themselves into all types of somatic cells, and into a line of genital cells. These stem cells are also capable of reproducing themselves over the whole lifespan of the organism. The participation of pluripotent stem cells and the possible mediation of antigen-presenting cells and T-helpers/T-suppressors in the complex with molecules of the MHC I class/II class make it possible to consider that exactly this immune system is responsible for regeneration of tissues in the organism. The participation in the regeneration process is the most important (and perhaps the leading) function of the immune system. With age the quantity of pluripotent stem cells gradually decreases. It leads to violation of renewal of tissues at people over 35-40 years old. Transfusion of mononuclear fraction of peripheral blood procured from young donors 18-23 years old with the same blood groups and sex as the recipient (RF patent number 2350340), allows people over 40-50 years old to reestablish the pool of pluripotent stem cells and the process of tissue renewal.","PeriodicalId":53626,"journal":{"name":"Journal of Stem Cells","volume":"11 2","pages":"69-87"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34814730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platelet-Rich Plasma Growth Factor Concentrated Spray (Keratogrow®) as a Potential Treatment for Androgenic Alopecia. 富血小板血浆生长因子浓缩喷雾(keratgrow®)作为雄激素性脱发的潜在治疗方法。

Journal of Stem Cells Pub Date : 2016-01-01

Rani James, Rosy Chetry, Vignesh Subramanian, Abhishek Ashtekar, Nandagiri Srikruthi, Sankar Ramachandran, Prasad S Koka, Kaushik Deb

引用次数: 0