Journal of Stem Cells最新文献

In Vivo Evaluation and Safety of the Wound-Healing Capability of Electrospun PCL/Gelatin Nanofibers Seeded with Wharton Jelly Mesenchymal Stem Cells 华顿果冻间充质干细胞电纺PCL/明胶纳米纤维伤口愈合能力的体内评价和安全性

Journal of Stem Cells Pub Date : 2022-01-01 DOI: 10.52305/qzay5079

S. Narayanan, P. Suresh

引用次数: 1

Migration of Neural Stem Cells in Hippocampal Slices in Hypoxia Modeling 神经干细胞在缺氧模型海马切片中的迁移

Journal of Stem Cells Pub Date : 2017-12-17 DOI: 10.28967/jscrt.2016.01.16002

Yulia Stukach, Khalil x Gainutdinov, M. Dosina, S. Pashkevich, V. Andrianov, A. Denisov, T. Bogodvid, G. Yafarova, Sergei Bushuk, T. Kuznetsova, V. Kulchitsky

引用次数: 4

Cheap and Simple: Human Tube Mesenchymal Stem Cells as Feeder Layer for Human Embryonic Stem Cells 廉价和简单:人管间充质干细胞作为人胚胎干细胞的饲养层

Journal of Stem Cells Pub Date : 2016-12-29 DOI: 10.28967/JSCRT.2016.01.16003

M. Pelatti, M. Secco, G. Coatti, J. Gomes, M. Valadares, A. Assoni, M. Maluf, C. Czeresnia, P. Perin, M. Martins, M. Zatz, T. Jazedje

引用次数: 0

Multipotent Stem Cell and Reproduction. 多能干细胞与生殖。

Journal of Stem Cells Pub Date : 2016-01-01

Neda Khanlarkhani, Maryam Baazm, Farzaneh Mohammadzadeh, Atefeh Najafi, Shayesteh Mehdinejadiani, Aligholi Sobhani

引用次数: 0

Biodegradable Gelatin Methacrylate Gel as a Potential Scaffold for Bone Tissue Engineering of Canine Adipose-Derived Stem Cells. 可生物降解的甲基丙烯酸明胶凝胶作为犬脂肪干细胞骨组织工程的潜在支架。

Journal of Stem Cells Pub Date : 2016-01-01

Mansi K Aparnathi, Jagdish S Patel

{"title":"Biodegradable Gelatin Methacrylate Gel as a Potential Scaffold for Bone Tissue Engineering of Canine Adipose-Derived Stem Cells.","authors":"Mansi K Aparnathi, Jagdish S Patel","doi":"","DOIUrl":"","url":null,"abstract":"Therapeutic potential of adipose derived stem cells (ADSCs) has widely been explored for treatment of orthopedic ailments. Transplantation of cells encapsulated in a scaffold facilitates 3 dimensional modelling of the tissue for the cases where well-defined spatial distribution of cells is desired for implantation. Present study aims to encapsulate canine ADSCs (cADSCs) in biodegradable methacrylated gelatin gel (GelMA) scaffold followed by their osteogenic differentiation for fabrication of a three dimensional bone tissue construct. Different percentages (5, 10 and 20%) and different methacrylation levels of gel (GelMAhigh and GelMAlow) were tested for degradation. Porosity of 10% GelMA was compared by SEM imaging. Gels with the fastest degradation rate (5% GelMAhigh and GelMAlow) were chosen for cell encapsulation since degradation of scaffold is of prime importance when the gel is intended to be used for implantation. Finally, cADSCs encapsulated in 5% GelMAlow demonstrated best morphology and were differentiated osteogenically. We developed a modified protocol for isolation of RNA from cells encapsulated in GelMA. Osteogenic differentiation was affirmed by the presence of osteo-specific gene expression by reverse transcriptase PCR in addition to von Kossa staining of the construct. GelMA is an excellent biodegradable scaffold for encapsulation of cADSCs without altering their osteogenic potential. This osteo-induced cellular scaffold implant opens a new therapeutic horizon in the area of tissue engineering in orthopedics.","PeriodicalId":53626,"journal":{"name":"Journal of Stem Cells","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34815199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of STPK in Maintaining the Quiescent Nature of Human CD34⁺ Stem Cells. STPK在维持人CD34+干细胞静止特性中的作用

Journal of Stem Cells Pub Date : 2016-01-01

Manne Mudhu Sunitha, Lokanathan Srikanth, Pasupuleti Santhosh Kumar, Chodimella Chandrasekhar, Potukuchi Venkata Gurunadha Krishna Sarma

{"title":"Role of STPK in Maintaining the Quiescent Nature of Human CD34+ Stem Cells.","authors":"Manne Mudhu Sunitha, Lokanathan Srikanth, Pasupuleti Santhosh Kumar, Chodimella Chandrasekhar, Potukuchi Venkata Gurunadha Krishna Sarma","doi":"","DOIUrl":"","url":null,"abstract":"Haematopoietic stem cell normally exists in the hypoxic niche of bone marrow and in this high anaerobic condition phosphorylation is vital in understanding the stemness of these stem cells in bone marrow. Analysis of human aldehyde dehydrogenase (ALDH) and isocitrate dehydrogenase (IDH) we have observed the presence of serine threonine protein kinase (STPK) sites in the protein sequence of these enzymes conferring that functioning of ALDH and IDH is regulated largely by STPK through phosphorylation. Human CD34+ stem cells and mononuclear cells as a control isolated from peripheral blood and were propagated in DMEM media at 5% CO2, 95% humidity and at 37°C. Thus obtained cells showed high enzyme activity for STPK, ALDH and low enzyme activity for IDH in CD34+ cells compare to control cells. These results were concurred with qRT-PCR studies with high gene expression levels of hypoxia inducing factor-1-alpha (HIF1α), STPK, ALDH and low IDH expression in CD34+ cells while normalized with β-actin. In addition the phosphorylating sites on ALDH and IDH proteins were identified and their importance in maintaining the anaerobic conditions in HSCs was demonstrated. In view of the importance of STPK signalling in the present study mechanism in cell division was addressed with phosphorylation of key regulating enzymes in the metabolic pathway of cell cycle was explored.","PeriodicalId":53626,"journal":{"name":"Journal of Stem Cells","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34815201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Short Study Report on Bone Marrow Aspirate Concentrate Cell Therapy in Ten South Asian Indian Patients with Autism. 10例南亚印度自闭症患者骨髓抽吸浓缩细胞治疗的简短研究报告。

Journal of Stem Cells Pub Date : 2016-01-01

Himanshu Bansal, Poonam Verma, Anupama Agrawal, Jerry Leon, I Birgitta Sundell, Prasad S Koka

引用次数: 0

Efficacy of Activated 3X Platelet-Rich Plasma in the Treatment of Androgenic Alopecia. 活化3X富血小板血浆治疗雄激素性脱发的疗效观察。

Journal of Stem Cells Pub Date : 2016-01-01

Rani James, Rosy Chetry, Vignesh Subramanian, Abhishek Ashtekar, Nandagiri Srikruthi, Sankar Ramachandran, Prasad S Koka, Kaushik Deb

引用次数: 0

Administration of Autologous Bone Marrow-Derived Stem Cells for Treatment of Cerebral Palsy Patients: A Proof of Concept. 自体骨髓源性干细胞治疗脑瘫患者:概念验证。

Journal of Stem Cells Pub Date : 2016-01-01

Himanshu Bansal, Lipi Singh, Poonam Verma, Anupama Agrawal, Jerry Leon, I Birgitta Sundell, Prasad S Koka

{"title":"Administration of Autologous Bone Marrow-Derived Stem Cells for Treatment of Cerebral Palsy Patients: A Proof of Concept.","authors":"Himanshu Bansal, Lipi Singh, Poonam Verma, Anupama Agrawal, Jerry Leon, I Birgitta Sundell, Prasad S Koka","doi":"","DOIUrl":"","url":null,"abstract":"Background: Stem cell therapy is a promising treatment for cerebral palsy, which refers to a category of brain diseases that are associated with chronic motor disability in children. Autologous bone marrow stem cells may be a better cell source and have been studied for the treatment of cerebral palsy because of their functions in tissue repair and the regulation of immunological processes.Methods: To assess autologous marrow stem cells as a novel treatment for patients with moderate-to-severe cerebral palsy, a total of 10 cerebral palsy patients were enrolled in this clinical study with 24 months follow-up. A total of 10 cerebral palsy patients received autologous bone marrow cells transplantation (4.5 × 108 mononuclear cells; 90% viability) into the subarachnoid cavity and rehabilitation.Results: We recorded the gross motor function measurement scores, manual ability function measurement score, and adverse events up to 24 months post-treatment. The gross motor function measurement scores were significantly higher at month 6 post-treatment compared with the baseline scores and were stable up to 24 months follow-up. The increase in manual ability and communication function measurement scores at 6 months were not significant when compared to the baseline score. All the 10 patients survived and none of the patients experienced any serious adverse events or complications.Conclusion: Our results indicated that bone marrow derived MNCs are safe and effective for the treatment of motor deficits related to cerebral palsy. Further randomized clinical trials are necessary to establish the efficacy of this procedure.","PeriodicalId":53626,"journal":{"name":"Journal of Stem Cells","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34814818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial - Autologous Bone Marrow Transplantations in Neurological Disorders. 编辑-自体骨髓移植治疗神经系统疾病。

Journal of Stem Cells Pub Date : 2016-01-01

Himanshu Bansal

引用次数: 0