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Monoclonal Antibodies in Immunodiagnosis and Immunotherapy最新文献

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Epitope Mapping of an Anti-ferret Podoplanin Monoclonal Antibody Using the PA Tag-Substituted Analysis. 利用 PA 标签替代分析法绘制抗铁锈色斑鬼臼毒素单克隆抗体的表位图。
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2023-12-01 DOI: 10.1089/mab.2023.0026
Yu Isoda, Mika K Kaneko, Tomohiro Tanaka, Hiroyuki Suzuki, Yukinari Kato
{"title":"Epitope Mapping of an Anti-ferret Podoplanin Monoclonal Antibody Using the PA Tag-Substituted Analysis.","authors":"Yu Isoda, Mika K Kaneko, Tomohiro Tanaka, Hiroyuki Suzuki, Yukinari Kato","doi":"10.1089/mab.2023.0026","DOIUrl":"10.1089/mab.2023.0026","url":null,"abstract":"<p><p>In small animal models of severe acute respiratory syndrome coronaviruses (SARS-CoV and SARS-CoV-2) infection, ferrets (<i>Mustela putorius furo</i>) have been used to investigate the pathogenesis. Podoplanin (PDPN) is an essential marker in lung type I alveolar epithelial cells, kidney podocytes, and lymphatic endothelial cells. Monoclonal antibodies (mAbs) against ferret PDPN (ferPDPN) are useful for the pathological analyses of those tissues. We previously established an anti-ferPDPN mAb, PMab-292 using the Cell-Based Immunization and Screening (CBIS) method. In this study, we determined the critical epitope of PMab-292 using flow cytometry. The ferPDPN deletion mutants analysis revealed that the Val34 is located at the N-terminus of the PMab-292 epitope. Furthermore, the PA tag-substituted analysis (PA scanning) showed that Asp39 is located at the C-terminus of PMab-292 epitope. The epitope sequence (VRPEDD) also exists between Val26 and Asp31 of ferPDPN, indicating that PMab-292 recognizes the tandem repeat of the VRPEDD sequence of ferPDPN.</p>","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":"42 6","pages":"189-193"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139075804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PMab-301: An Anti-Giraffe Podoplanin Monoclonal Antibody for Immunohistochemistry. PMab-301：一种用于免疫组化的抗吉拉夫波多波宁单克隆抗体。
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2023-12-01 Epub Date: 2023-12-26 DOI: 10.1089/mab.2023.0020
Tsunenori Ouchida, Tomohiro Tanaka, Hiroyuki Suzuki, Kazuyuki Uchida, Takayuki Nakagawa, Guanjie Li, Takuro Nakamura, Miyuki Yanaka, Saori Handa, Mika K Kaneko, Yukinari Kato
{"title":"PMab-301: An Anti-Giraffe Podoplanin Monoclonal Antibody for Immunohistochemistry.","authors":"Tsunenori Ouchida, Tomohiro Tanaka, Hiroyuki Suzuki, Kazuyuki Uchida, Takayuki Nakagawa, Guanjie Li, Takuro Nakamura, Miyuki Yanaka, Saori Handa, Mika K Kaneko, Yukinari Kato","doi":"10.1089/mab.2023.0020","DOIUrl":"10.1089/mab.2023.0020","url":null,"abstract":"<p><p>Immunohistochemistry staining is an essential method in pathological diagnoses. Podoplanin (PDPN) is a specific maker of alveolar epithelium, lymphatic vessels, and glomeruli. In this study, we established a novel anti-giraffe PDPN (girPDPN) mAb, PMab-301, using the Cell-Based Immunization and Screening (CBIS) method. PMab-301 (mouse IgG<sub>1</sub>, kappa) detected girPDPN in various applications, such as flow cytometry, western blot, and immunohistochemistry. PMab-301 specifically stained type-I alveolar cells using formalin-fixed paraffin-embedded giraffe lung tissues. Our findings suggest the potential usefulness of PMab-301 for the pathophysiological analyses of giraffe tissues.</p>","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":" ","pages":"209-215"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139040900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Is It Time to Re-Evaluate? 是时候重新评估了吗？
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2023-12-01 Epub Date: 2023-12-22 DOI: 10.1089/mab.2023.29016.editorial
Thomas Kieber-Emmons
{"title":"Is It Time to Re-Evaluate?","authors":"Thomas Kieber-Emmons","doi":"10.1089/mab.2023.29016.editorial","DOIUrl":"10.1089/mab.2023.29016.editorial","url":null,"abstract":"","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":" ","pages":"187-188"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138832974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Monoclonal Antibodies 4B11 and 6D7 Against SARS-CoV-2 Nucleocapsid Protein. 针对 SARS-CoV-2 核壳蛋白的 4B11 和 6D7 单克隆抗体。
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2023-12-01 DOI: 10.1089/mab.2023.0028
{"title":"Monoclonal Antibodies 4B11 and 6D7 Against SARS-CoV-2 Nucleocapsid Protein.","authors":"","doi":"10.1089/mab.2023.0028","DOIUrl":"10.1089/mab.2023.0028","url":null,"abstract":"","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":"42 6","pages":"217"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139075805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Rat Anti-Mouse CD39 Monoclonal Antibody for Flow Cytometry. 用于流式细胞仪的大鼠抗小鼠 CD39 单克隆抗体。
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2023-12-01 Epub Date: 2023-12-21 DOI: 10.1089/mab.2023.0018
Hiroyuki Suzuki, Tomohiro Tanaka, Yuma Kudo, Mayuki Tawara, Aoi Hirayama, Mika K Kaneko, Yukinari Kato
{"title":"A Rat Anti-Mouse CD39 Monoclonal Antibody for Flow Cytometry.","authors":"Hiroyuki Suzuki, Tomohiro Tanaka, Yuma Kudo, Mayuki Tawara, Aoi Hirayama, Mika K Kaneko, Yukinari Kato","doi":"10.1089/mab.2023.0018","DOIUrl":"10.1089/mab.2023.0018","url":null,"abstract":"<p><p>By converting extracellular adenosine triphosphate to adenosine, CD39 is involved in adenosine metabolism. The extracellular adenosine plays a critical role in the immune suppression of the tumor microenvironment. Therefore, the inhibition of CD39 activity by monoclonal antibodies (mAbs) is thought to be one of the important strategies for tumor therapy. In this study, we developed novel mAbs for mouse CD39 (mCD39) using the Cell-Based Immunization and Screening (CBIS) method. One of the established anti-mCD39 mAbs, C<sub>39</sub>Mab-2 (rat IgG<sub>2a</sub>, lambda), reacted with mCD39-overexpressed Chinese hamster ovary-K1 (CHO/mCD39) and an endogenously mCD39-expressed cell line (SN36) by flow cytometry. The kinetic analysis using flow cytometry indicated that the dissociation constant (<i>K</i><sub>D</sub>) values of C<sub>39</sub>Mab-2 for CHO/mCD39 and SN36 were 5.5 × 10<sup>-9</sup> M and 4.9 × 10<sup>-9</sup> M, respectively. These results indicated that C<sub>39</sub>Mab-2 is useful for the detection of mCD39 in flow cytometry.</p>","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":" ","pages":"203-208"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138832972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Monoclonal Antibody for Recombinant Histidine Rich Protein 3 (HRP 3) of Plasmodium falciparam. 恶性疟原虫重组富组氨酸蛋白 3 (HRP 3) 的单克隆抗体。
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2023-12-01 Epub Date: 2021-06-24 DOI: 10.1089/mab.2018.0013
{"title":"Monoclonal Antibody for Recombinant Histidine Rich Protein 3 (HRP 3) of <i>Plasmodium falciparam</i>.","authors":"","doi":"10.1089/mab.2018.0013","DOIUrl":"10.1089/mab.2018.0013","url":null,"abstract":"","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":" ","pages":"216"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39106258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Case Report of Graves' Disease in a 45-Year-Old Woman Secondary to Herceptin Treatment for Breast Cancer. 乳腺癌赫赛汀治疗后继发 45 岁女性巴塞杜氏病的病例报告。
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2023-12-01 DOI: 10.1089/mab.2023.0011
Chinmeri U Achonu, Oluwarotimi B Olopade, Bashir O Yusuf, Abimbola A Fadeyi, Olufemi A Fasanmade
{"title":"Case Report of Graves' Disease in a 45-Year-Old Woman Secondary to Herceptin Treatment for Breast Cancer.","authors":"Chinmeri U Achonu, Oluwarotimi B Olopade, Bashir O Yusuf, Abimbola A Fadeyi, Olufemi A Fasanmade","doi":"10.1089/mab.2023.0011","DOIUrl":"10.1089/mab.2023.0011","url":null,"abstract":"<p><p>Graves' disease is the most common cause of thyrotoxicosis and is characterized by ophthalmopathy with proptosis, chemosis, or conjunctival injection; pretibial myxedema; and thyroid acropachy. It is an autoimmune disease that can be genetic or influenced by coexisting environmental factors such as exposure to anticancer drugs, including immune checkpoint inhibitors. The incidence rate of breast cancer is increasing due to rising awareness of risk factors and screening for breast cancer, and the mortality rate is decreasing due to recent advances in cancer treatment. However, there are side effects that are attributed to these treatment modalities, manifesting in various forms in breast cancer survivors, which are reflected in the patient in this case study.</p>","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":"42 6","pages":"194-202"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139075803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and Characterization of a Novel Neuropilin-2 Antibody for Immunohistochemical Staining of Cancer and Sarcoidosis Tissue Samples. 用于癌症和结节病组织样品免疫组织化学染色的新型Neuropilin-2抗体的开发和表征。
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2023-10-01 DOI: 10.1089/mab.2023.0007
Sarah Förster, Yeeting E Chong, David Siefker, Yvonne Becker, Ruizhi Bao, Erik Escobedo, Yang Qing, Kaitlyn Rauch, Luke Burman, Christoph Burkart, Philipp Kainz, Andrea Cubitt, Michael Muders, Leslie A Nangle
{"title":"Development and Characterization of a Novel Neuropilin-2 Antibody for Immunohistochemical Staining of Cancer and Sarcoidosis Tissue Samples.","authors":"Sarah Förster,&nbsp;Yeeting E Chong,&nbsp;David Siefker,&nbsp;Yvonne Becker,&nbsp;Ruizhi Bao,&nbsp;Erik Escobedo,&nbsp;Yang Qing,&nbsp;Kaitlyn Rauch,&nbsp;Luke Burman,&nbsp;Christoph Burkart,&nbsp;Philipp Kainz,&nbsp;Andrea Cubitt,&nbsp;Michael Muders,&nbsp;Leslie A Nangle","doi":"10.1089/mab.2023.0007","DOIUrl":"10.1089/mab.2023.0007","url":null,"abstract":"<p><p>Neuropilin-2 (NRP2) is a cell surface receptor that plays key roles in lymphangiogenesis, but also in pathophysiological conditions such as cancer and inflammation. NRP2 targeting by efzofitimod, a novel immunomodulatory molecule, is currently being tested for the treatment of pulmonary sarcoidosis. To date, no anti-NRP2 antibodies are available for companion diagnostics. Here we describe the development and characterization of a novel NRP2 antibody. Using a variety of research techniques, that is, enzyme-linked immunoassay, Western blot, biolayer interferometry, and immunohistochemistry, we demonstrate that our antibody detects all major NRP2 isoforms and does not cross-react with NRP1. Using this antibody, we show high NRP2 expression in granulomas from sarcoidosis patient skin and lung biopsies. Our novel anti-NRP2 antibody could prove to be a useful clinical tool for sarcoidosis and other indications where NRP2 has been implicated. <b><i>Clinical Trial Registration:</i></b> clinicaltrials.gov NCT05415137.</p>","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":"42 5","pages":"157-165"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71415200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MAb Targeting a Link Between ExoN and MTase of TGEV NSP14. 靶向TGEV NSP14的外显子N和MTase之间连接的MAb。
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2023-10-01 Epub Date: 2023-10-19 DOI: 10.1089/mab.2023.0010
Lilan Xie, Fang Lin, Peiling Dong, Yaoming Li
{"title":"MAb Targeting a Link Between ExoN and MTase of TGEV NSP14.","authors":"Lilan Xie, Fang Lin, Peiling Dong, Yaoming Li","doi":"10.1089/mab.2023.0010","DOIUrl":"10.1089/mab.2023.0010","url":null,"abstract":"<p><p>Porcine transmissible gastroenteritis virus (TGEV) infection results in severe gastrointestinal disease manifesting vomiting, diarrhea in neonatal porcine, with extremely high mortality. Monoclonal antibody (MAb) specific to TGEV nonstructural protein (NSP)14 that contains two functional domains, exonuclease (ExoN) and methyltransferase (MTase) domains, may help elucidate the role of NSP14 in the viral life-cycle. In this study, we developed a murine MAb, designated 12F1, against TGEV NSP14 using traditional cell-fusion technique. It was shown the MAb can exclusively bind to viral NSP14, as evidenced by the results of indirect fluorescent assay and western blotting. Intriguingly, epitope screening assay shown that 12F1 targets a hinge region connecting ExoN and N7-MTase of NSP14.</p>","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":" ","pages":"178-181"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10621669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49684936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of Novel Monoclonal Antibodies Against Nattokinase. 纳豆激酶单克隆抗体的研制。
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2023-10-01 Epub Date: 2023-10-19 DOI: 10.1089/mab.2023.0012
Takashi Tanikawa, James Yu, Kate Hsu, Shinder Chen, Ayako Ishii, Takami Yokogawa, Yutaka Inoue, Masashi Kitamura
{"title":"Development of Novel Monoclonal Antibodies Against Nattokinase.","authors":"Takashi Tanikawa,&nbsp;James Yu,&nbsp;Kate Hsu,&nbsp;Shinder Chen,&nbsp;Ayako Ishii,&nbsp;Takami Yokogawa,&nbsp;Yutaka Inoue,&nbsp;Masashi Kitamura","doi":"10.1089/mab.2023.0012","DOIUrl":"10.1089/mab.2023.0012","url":null,"abstract":"<p><p>Nattokinase is a protease produced by <i>Bacillus subtilis</i> var. <i>natto</i> that exhibits various beneficial biological effects. Thus, a reliable assay to determine nattokinase levels is needed. In this study, we developed novel mouse monoclonal antibodies (mAbs) that recognize nattokinase, and created a specific and sensitive enzyme-linked immunosorbent assay (ELISA) to measure nattokinase levels. The ELISA was developed using a combination of new mouse antinattokinase mAbs used as capture antibodies coated onto 96-well plates, with a peroxidase-conjugated antibody used for detection. This ELISA enabled detection of nattokinase at 1 ng/mL. We believe that the novel mAbs developed in this study will be useful in future for elucidating nattokinase function.</p>","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":" ","pages":"153-156"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49684935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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