Monoclonal Antibodies in Immunodiagnosis and Immunotherapy最新文献

How Alternative Models May be of Assistance in Antibody Development. 如何替代模型可能有助于抗体开发。

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2026-05-01 Epub Date: 2026-02-19 DOI: 10.1177/21679436261425534

Kelvin Sousa Dos Santos, Cory L Brooks, Andrei Moroz

引用次数: 0

A Novel Anti-CDH5/VE-Cadherin Monoclonal Antibody (Ca₅Mab-8) for Flow Cytometry, Western Blotting, and Immunohistochemistry. 一种新型抗cdh5 /VE-Cadherin单克隆抗体（Ca5Mab-8），用于流式细胞术、Western Blotting和免疫组织化学。

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2026-05-01 Epub Date: 2026-04-09 DOI: 10.1177/21679436261441142

Haruto Yamamoto, Hiroyuki Suzuki, Mika K Kaneko, Yukinari Kato

{"title":"A Novel Anti-CDH5/VE-Cadherin Monoclonal Antibody (Ca5Mab-8) for Flow Cytometry, Western Blotting, and Immunohistochemistry.","authors":"Haruto Yamamoto, Hiroyuki Suzuki, Mika K Kaneko, Yukinari Kato","doi":"10.1177/21679436261441142","DOIUrl":"10.1177/21679436261441142","url":null,"abstract":"Cadherin-5 (CDH5), also known as vascular endothelial cadherin (VE-cadherin), plays crucial roles in endothelial cell adhesion, vascular barrier function, and signaling. CDH5 manages endothelial cell-cell junctions during vascular remodeling, which is vital for both vascular homeostasis and adaptive responses to pathological stimuli. Although anti-CDH5 monoclonal antibodies (mAbs) are used for specific applications such as flow cytometry, Western blotting, and immunohistochemistry (IHC), highly sensitive and versatile anti-CDH5 mAbs suitable for all these applications remain limited. Here, new anti-human CDH5 mAbs, called Ca5Mabs, were developed through a flow cytometry-based high-throughput screening. Among them, clone Ca5Mab-8 (mouse IgG2a, kappa) recognized CDH5-overexpressed Chinese hamster ovary-K1 (CHO/CDH5) cells in flow cytometry. Moreover, Ca5Mab-8 also recognized endogenous CDH5-expressing human endothelial cell lines (HUVEC/TERT2 and HDMVEC/TERT164-B) and a cervical cancer cell line (HeLa). These reactivities exceeded those of a commercial anti-CDH5 mAb (clone BV9). The apparent dissociation constant of Ca5Mab-8 for CHO/CDH5 was measured as 6.1 × 10-9 M. Ca5Mab-8 can detect endogenous CDH5 in Western blotting. In addition, Ca5Mab-8, but not BV9, can be used for IHC to detect endothelial cells in formalin-fixed paraffin-embedded tissues. These findings suggest that Ca5Mab-8 is a versatile basic research tool in vascular biology and has potential for clinical applications including tumor diagnosis and therapy.","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":" ","pages":"34-44"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147640400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Anti-Human Cadherin-26 Monoclonal Antibody, Ca₂₆Mab-6, for Flow Cytometry. 用于流式细胞术的抗人钙粘蛋白26单克隆抗体Ca26Mab-6的研制

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2026-05-01 Epub Date: 2026-03-09 DOI: 10.1177/21679436261428408

Hiroyuki Satofuka, Hiroyuki Suzuki, Mika K Kaneko, Yukinari Kato

{"title":"Development of Anti-Human Cadherin-26 Monoclonal Antibody, Ca26Mab-6, for Flow Cytometry.","authors":"Hiroyuki Satofuka, Hiroyuki Suzuki, Mika K Kaneko, Yukinari Kato","doi":"10.1177/21679436261428408","DOIUrl":"10.1177/21679436261428408","url":null,"abstract":"Cadherin 26 (CDH26) is a recently identified member of the cadherin superfamily. Although CDH26 gene expression has been reported in association with allergic inflammatory responses, the protein expression levels and the signaling pathways mediated through its interactions with other proteins remain poorly understood. This is primarily due to the lack of monoclonal antibodies (mAbs) that can recognize the intact, cell surface-expressed form of CDH26. In this study, we developed an antihuman CDH26 mAb, Ca26Mab-6 (IgM, kappa), using the Cell-Based Immunization and Screening (CBIS) method. Ca26Mab-6 demonstrated high sensitivity and specificity for CDH26 in flow cytometry and did not bind to Chinese hamster ovary (CHO)-K1 cells, which overexpress any of the other type I or type II cadherins. Ca26Mab-6 successfully detected endogenous CDH26 protein expression in HepG2, U-87 MG, MCF7, and 293FT cells. The apparent dissociation constant of Ca26Mab-6 was determined to be 9.8 ± 4.8 × 10-9 M for CDH26-overexpressed CHO-K1 (CHO/CDH26) cells and 3.6 ± 1.0 × 10-7 M for HepG2 cells. The detection of CDH26 expression in cancer cells may offer new insights into the potential relationship between inflammatory responses and malignant transformation. Therefore, Ca26Mab-6, developed using the CBIS method, is expected to facilitate functional studies of CDH26 and contribute to the development of CDH26-targeted antibody-based therapies.","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":" ","pages":"25-33"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147391661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acknowledgment of Reviewers 2025. 审稿人致谢2025。

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2026-04-15 DOI: 10.1177/21679436261431081

引用次数: 0

Cx₂Mab-5: A Novel Anti-Mouse CXCR2 Monoclonal Antibody Developed by N-Terminus Peptide Immunization for Multiple Applications. Cx2Mab-5：一种n端肽免疫多用途抗小鼠CXCR2单克隆抗体

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2026-02-01 Epub Date: 2026-02-19 DOI: 10.1177/21679436251415261

Honoka Taruta, Tomohiro Tanaka, Mika K Kaneko, Hiroyuki Suzuki, Yukinari Kato

{"title":"Cx2Mab-5: A Novel Anti-Mouse CXCR2 Monoclonal Antibody Developed by N-Terminus Peptide Immunization for Multiple Applications.","authors":"Honoka Taruta, Tomohiro Tanaka, Mika K Kaneko, Hiroyuki Suzuki, Yukinari Kato","doi":"10.1177/21679436251415261","DOIUrl":"10.1177/21679436251415261","url":null,"abstract":"The CXC chemokine receptor 2 (CXCR2) is a member of the G-protein-coupled receptor superfamily and regulates a diverse range of immune responses and tumor progression. CXCR2 is expressed on immune cells, especially neutrophils, and is involved in various immune responses by interacting with its chemokine ligands. Therefore, the development of sensitive monoclonal antibodies (mAbs) for CXCR2 has been desired for treatment and diagnosis. This study established a novel sensitive anti-mouse CXCR2 (mCXCR2) mAb; Cx2Mab-5 (rat IgG2a, κ), using the mCXCR2 synthetic N-terminus peptide immunization method. In flow cytometry, Cx2Mab-5 recognized mCXCR2-overexpressed Chinese hamster ovary-K1 cells (CHO/mCXCR2) and WEHI-3B (murine myelomonocytic leukemia cell) cells, which express endogenous mCXCR2. Cx2Mab-5 did not cross-react with other mouse CC, CXC, CX3C, and XC chemokine receptors. Cx2Mab-5 showed a moderate binding affinity for both CHO/mCXCR2 and WEHI-3B. Furthermore, Cx2Mab-5 detected mCXCR2 in Western blot and immunohistochemistry in CHO/mCXCR2 cells, but a commercially available anti-mCXCR2 mAb (clone SA045E1) did not. Hence, Cx2Mab-5 can be a valuable tool for analyzing mCXCR2-positive cells in mouse tissues.","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":" ","pages":"11-20"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146222087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of Novel Anti-EphB3 Monoclonal Antibodies for Multiple Applications. 新型多用途抗ephb3单克隆抗体的建立

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2026-02-01 Epub Date: 2026-01-28 DOI: 10.1177/21679436251408319

Guanjie Li, Hiroyuki Suzuki, Mika K Kaneko, Yukinari Kato

{"title":"Establishment of Novel Anti-EphB3 Monoclonal Antibodies for Multiple Applications.","authors":"Guanjie Li, Hiroyuki Suzuki, Mika K Kaneko, Yukinari Kato","doi":"10.1177/21679436251408319","DOIUrl":"10.1177/21679436251408319","url":null,"abstract":"Ephrin type-B receptor 3 (EphB3) binds to transmembrane ephrin-B ligands to regulate cell migration, adhesion, and proliferation. EphB3 exhibits a gradient expression pattern in the normal intestine, with the highest levels at the crypt base, and plays a crucial role in the maintenance of normal intestinal epithelium. Therefore, anti-EphB3 monoclonal antibodies (mAbs) are required for basic research and diagnosis. In this study, we developed novel antihuman EphB3, Eb3Mab-5 (IgG1, κ) and Eb3Mab-11 (IgG1, κ), using the Cell-Based Immunization and Screening (CBIS) method. Eb3Mab-5 and Eb3Mab-11 reacted with EphB3-overexpressed Chinese hamster ovary-K1 (CHO/EphB3) and endogenous EphB3-positive colorectal cancer LS174T in flow cytometry. The apparent binding affinity of Eb3Mab-5 for CHO/EphB3 and LS174T was 7.6 × 10-9 M and 1.7 × 10-8 M, respectively. Eb3Mab-11 could detect EphB3 in western blot analysis and immunohistochemistry. Eb3Mab-5 and Eb3Mab-11, established by the CBIS method, may contribute to the diagnosis and therapy of EphB3-positive tumors.","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145858974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of Rat Monoclonal Antibodies for Human and Mouse Androgen Receptor. 人与小鼠雄激素受体大鼠单克隆抗体的制备。

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2025-12-01 Epub Date: 2025-11-19 DOI: 10.1177/21679436251398987

Momo Koike, Tomoka Kawabata, Norikazu Kiguchi, Yu Hatano, Kentaro Suzuki, Taro Tachibana, Chikako Yokoyama

引用次数: 0

Equitable Access to Antibody Libraries to Accelerate Discovery. 公平获取抗体库加速发现。

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2025-12-01 DOI: 10.1177/21679436251404827

Andrei Moroz, Cory L Brooks

引用次数: 0

The Development of Monoclonal Antibodies Specific to the Soluble Form of CD155 in the Human Peripheral Blood. 人外周血可溶型CD155特异性单克隆抗体的研制

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2025-10-01 DOI: 10.1177/21679436251390201

Shota Kinoshita, Tomohei Matsuo, Soontae Gwon, Naoto Takeuchi, Akiko Iguchi-Manaka, Chigusa Nakahashi-Oda, Akira Shibuya, Kazuko Shibuya

{"title":"The Development of Monoclonal Antibodies Specific to the Soluble Form of CD155 in the Human Peripheral Blood.","authors":"Shota Kinoshita, Tomohei Matsuo, Soontae Gwon, Naoto Takeuchi, Akiko Iguchi-Manaka, Chigusa Nakahashi-Oda, Akira Shibuya, Kazuko Shibuya","doi":"10.1177/21679436251390201","DOIUrl":"https://doi.org/10.1177/21679436251390201","url":null,"abstract":"CD155, also known as poliovirus receptor (PVR) or Necl-5, is an immunoreceptor with three immunoglobulin-like domains in the extracellular portion and is ubiquitously expressed on the cell surface of hematopoietic and nonhematopoietic cells. Human CD155 contains membrane-bound CD155 (mCD155) and soluble CD155 (sCD155) lacking the transmembrane region encoded by splicing variants of genes. The serum levels of sCD155 increased in patients with a variety of cancers. Furthermore, sCD155 suppresses tumor immunity through the blockade of DNAM-1 signaling. Therefore, sCD155 is potentially helpful for the diagnosis of cancer development and a novel therapeutic target in cancer treatment. However, monoclonal antibodies (mAbs) specific to sCD155, but not mCD155, have not yet been developed. Here, we generated 14 mAbs (named TX119 to TX122 and TX126 to TX135) that recognize the cytoplasmic region of CD155. These mAbs bind to sCD155, but not mCD155, when applied from the outside of the cell. Moreover, we established the assay system to quantify the sCD155 concentration in human serum. Thus, these mAbs can be utilized for the targeting and quantification of sCD155 in the human peripheral blood.","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":"44 5","pages":"79-83"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145294236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stunning Progress in De Novo Computationally Designed Antibodies. 计算设计抗体的惊人进展。

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2025-10-01 DOI: 10.1177/21679436251385401

Andrei Moroz, Cory L Brooks

引用次数: 0