Marine Life Science & Technology最新文献

{"title":"Contribution of the SOS response and the DNA repair systems to norfloxacin induced mutations in E. coli","authors":"Tongtong Lin, Jiao Pan, Colin Gregory, Yaohai Wang, Clayton Tincher, Caitlyn Rivera, Michael Lynch, Hongan Long, Yu Zhang","doi":"10.1007/s42995-023-00185-y","DOIUrl":"https://doi.org/10.1007/s42995-023-00185-y","url":null,"abstract":"Abstract Antibiotic-resistant bacteria severely threaten human health. Besides spontaneous mutations generated by endogenous factors, the resistance might also originate from mutations induced by certain antibiotics, such as the fluoroquinolones. Such antibiotics increase the genome-wide mutation rate by introducing replication errors from the SOS response pathway or decreasing the efficiency of the DNA repair systems. However, the relative contributions of these molecular mechanisms remain unclear, hindering understanding of the generation of resistant pathogens. Here, using newly-accumulated mutations of wild-type and SOS-uninducible Escherichia coli strains, as well as those of the strains deficient for the mismatch repair (MMR) and the oxidative damage repair pathways, we find that the SOS response is the major mutagenesis contributor in mutation elevation, responsible for ~ 30–50% of the total base-pair substitution (BPS) mutation-rate elevation upon treatment with sublethal levels of norfloxacin (0 ~ 50 ng/mL). We further estimate the significance of the effects on other mutational features of these mechanisms (i.e., transversions, structural variations, and mutation spectrum) in E. coli using linear models. The SOS response plays a positive role in all three mutational features (mutation rates of BPSs, transversions, structural variations) and affects the mutational spectrum. The repair systems significantly reduce the BPS mutation rate and the transversion rate, regardless of whether antibiotics are present, while significantly increasing the structural variation rate in E. coli . Our results quantitatively disentangle the contributions of the SOS response and DNA repair systems in antibiotic-induced mutagenesis.","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On four epibiotic peritrichous ciliates (Protozoa, Ciliophora) found in Lake Weishan Wetland: morphological and molecular data support the establishment of a new genus, <i>Parapiosoma</i> gen. nov., and two new species.","authors":"Tong Wu,&nbsp;Ting Cheng,&nbsp;Xiao Cao,&nbsp;Yaohan Jiang,&nbsp;Khaled A S Al-Rasheid,&nbsp;Alan Warren,&nbsp;Zhe Wang,&nbsp;Borong Lu","doi":"10.1007/s42995-023-00184-z","DOIUrl":"10.1007/s42995-023-00184-z","url":null,"abstract":"<p><p>During a study on the diversity of ciliated protists in Lake Weishan Wetland, the largest wetland in northern China, four epibiotic sessilid peritrichs were isolated from aquatic host animals. Two of them, i.e., <i>Epistylis cambari</i> Kellicott, 1885 and <i>Epistylis lwoffi</i> Fauré-Fremiet, 1943, were known species whereas the other two, i.e., <i>Parapiosoma typicum</i> gen. nov., sp. nov. and <i>Orborhabdostyla gracilis</i> sp. nov., are new to science. The new genus <i>Parapiosoma</i> gen. nov. is characterized by its branched non-contractile stalk, everted peristomial lip, obconical macronucleus and transverse silverlines. Two species are assigned to the new genus, namely <i>Parapiosoma typicum</i> sp. nov. and <i>Parapiosoma gasterostei</i> (Fauré-Fremiet, 1905) comb. nov. Morphologically, <i>P. typicum</i> sp. nov. is recognized by its goblet-shaped zooids, single-layered peristomial lip, dichotomously branched stalk, and infundibular polykinety 3 (P3) containing three equal-length rows. <i>Orborhabdostyla gracilis</i> sp. nov. is characterized by its slender zooid, curved macronucleus, and three equal-length rows in infundibular P3. Improved diagnoses and redescriptions of <i>E. cambari</i> and <i>E. lwoffi</i> are provided including, for the first time, data on the ciliature of <i>E. cambari</i>. Phylogenetic analyses based on SSU rDNA, ITS1-5.8S rDNA -ITS2, and LSU rDNA sequence data strongly support the assertion that the family Epistylididae comprises morphospecies with different evolutionary lineages and indicate that <i>Parapiosoma</i> gen. nov. may represent a new taxon at family level.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 3","pages":"337-358"},"PeriodicalIF":5.7,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449748/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10164512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Large yellow croaker (<i>Larimichthys crocea</i>) mitofusin 2 inhibits type I IFN responses by degrading MAVS via enhanced K48-linked ubiquitination.","authors":"Wen-Xing Li, Xiao-Hong Wang, Yi-Jun Lin, Yuan-Yuan Zhou, Jun Li, Xiang-Yang Zhang, Xin-Hua Chen","doi":"10.1007/s42995-023-00189-8","DOIUrl":"10.1007/s42995-023-00189-8","url":null,"abstract":"<p><p>In mammals, mitofusin 2 (MFN2) is involved in mitochondrial fusion, and suppresses the virus-induced RIG-I-like receptor (RLR) signaling pathway. However, little is known about the function of MFN2 in non-mammalian species. In the present study, we cloned an MFN2 ortholog (<i>Lc</i>MFN2) in large yellow croaker (<i>Larimichthys crocea</i>). Phylogenetic analysis showed that MFN2 emerged after the divergence of amphioxus and vertebrates. The protein sequences of MFN2 were well conserved from fish to mammals. <i>Lc</i>MFN2 was expressed in all the tissues/organs examined at different levels, and its expression was upregulated in response to poly(I:C) stimulation. Overexpression of <i>Lc</i>MFN2 inhibited MAVS-induced type I interferon (IFN) promoter activation and antiviral gene expression. In contrast, knockdown of endogenous <i>Lc</i>MFN2 enhanced poly(I:C) induced production of type I IFNs. Additionally, <i>Lc</i>MFN2 enhanced K48-linked polyubiquitination of MAVS, promoting its degradation. Also, overexpression of <i>Lc</i>MFN2 impaired the cellular antiviral response, as evidenced by the increased expression of viral genes and more severe cytopathic effects (CPE) in cells infected with spring viremia of carp virus (SVCV). These results indicated that <i>Lc</i>MFN2 inhibited type I IFN response by degrading MAVS, suggesting its negative regulatory role in cellular antiviral response. Therefore, our study sheds a new light on the regulatory mechanisms of the cellular antiviral response in teleosts.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00189-8.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 3","pages":"359-372"},"PeriodicalIF":5.8,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10110066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An improved blood hemorrhaging treatment using diatoms frustules, by alternating Ca and light levels in cultures.","authors":"Qinfeng Li,&nbsp;Zheng He,&nbsp;Hussein E Rozan,&nbsp;Chao Feng,&nbsp;Xiaojie Cheng,&nbsp;Xiguang Chen","doi":"10.1007/s42995-023-00180-3","DOIUrl":"10.1007/s42995-023-00180-3","url":null,"abstract":"<p><p>Hemorrhage control requires hemostatic materials that are both effective and biocompatible. Among these, diatom biosilica (DBs) could significantly improve hemorrhage control, but it induces hemolysis (the hemolysis rate > 5%). Thus, the purpose of this study was to explore the influence of Ca²⁺ biomineralization on DBs for developing fast hemostatic materials with a low hemolysis rate. Here, CaCl₂ was added to the diatom medium under high light (cool white, fluorescent lamps, 67.5 µmol m^-2 s^-1), producing Ca-DBs-3 with a particle size of 40-50 μm and a Ca²⁺ content of Ca-DBs-3 obtained from the higher concentration CaCl₂ group (6.7 mmol L^-1) of 0.16%. The liquid absorption capacity of Ca-DBs-3 was 30.43 ± 0.57 times its dry weight; the in vitro clotting time was comparable to QuikClot^® zeolite; the hemostatic time and blood loss using the rat tail amputation model were 36.40 ± 2.52 s and 0.39 ± 0.12 g, which were 40.72% and 19.50% of QuikClot^® zeolite, respectively. Ca-DBs-3 showed no apparent toxicity to L929 cells (cell viability > 80%) and was non-hemolysis (the hemolysis rate < 2%). This study prepared Ca-DBs-3 with a rapid hemostatic effect and good biocompatibility, providing a path to develop diatom biosilica hemostatic materials.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00180-3.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 3","pages":"316-325"},"PeriodicalIF":5.7,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10110067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression analyses of stress-responsive genes in the hermatypic coral <i>Acropora tenuis</i> and its symbiotic dinoflagellates after exposure to the herbicide Diuron.","authors":"Hiroshi Ishibashi, Seigo Minamide, Ichiro Takeuchi","doi":"10.1007/s42995-023-00183-0","DOIUrl":"10.1007/s42995-023-00183-0","url":null,"abstract":"<p><p>Diuron is one of the most frequently applied herbicides in sugarcane farming in southern Japan, and Australia. In addition, it is used as a booster substance in copper-based antifouling paints. Due to these various uses, Diuron is released into the marine environment; however, little information is available on gene expression in corals and their symbiotic algae exposed to Diuron. We investigated the effects of Diuron on stress-responsive gene expression in the hermatypic coral <i>Acropora tenuis</i> and its symbiotic dinoflagellates. After seven days of exposure to 1 µg/L and 10 µg/L Diuron, no significant changes in the body colour of corals were observed. However, quantitative reverse transcription-polymerase chain reaction analyses revealed that the expression levels of stress-responsive genes, such as heat shock protein 90 (<i>HSP90</i>), <i>HSP70</i>, and calreticulin (<i>CALR</i>), were significantly downregulated in corals exposed to 10 µg/L of Diuron for seven days. Moreover, aquaglyceroporin was significantly downregulated in corals exposed to environmentally relevant concentrations of 1 µg/L Diuron. In contrast, no such effects were observed on the expression levels of other stress-responsive genes, such as oxidative stress-responsive proteins, methionine adenosyltransferase, and green/red fluorescent proteins. Diuron exposure had no significant effect on the expression levels of <i>HSP90</i>, <i>HSP70</i>, or <i>HSP40</i> in the symbiotic dinoflagellates. These results suggest that stress-responsive genes, such as <i>HSP</i>s, respond differently to Diuron in corals and their symbiotic dinoflagellates and that <i>A. tenuis HSP</i>s and <i>CALR</i>s may be useful molecular biomarkers for predicting stress responses induced by the herbicide Diuron.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00183-0.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 3","pages":"289-299"},"PeriodicalIF":5.8,"publicationDate":"2023-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10164511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-chain fatty acids (SCFAs) as potential resuscitation factors that promote the isolation and culture of uncultured bacteria in marine sediments.","authors":"Chun-Shui Sun,&nbsp;Liu-Yan Zhou,&nbsp;Qi-Yun Liang,&nbsp;Xiao-Man Wang,&nbsp;Yi-Xuan Lei,&nbsp;Zhen-Xing Xu,&nbsp;Feng-Qing Wang,&nbsp;Guan-Jun Chen,&nbsp;Zong-Jun Du,&nbsp;Da-Shuai Mu","doi":"10.1007/s42995-023-00187-w","DOIUrl":"10.1007/s42995-023-00187-w","url":null,"abstract":"<p><p>Many marine bacteria are difficult to culture because they are dormant, rare or found in low-abundances. Enrichment culturing has been widely tested as an important strategy to isolate rare or dormant microbes. However, many more mechanisms remain uncertain. Here, based on 16S rRNA gene high-throughput sequencing and metabolomics technology, it was found that the short-chain fatty acids (SCFAs) in metabolites were significantly correlated with uncultured bacterial groups during enrichment cultures. A pure culture analysis showed that the addition of SCFAs to media also resulted in high efficiency for the isolation of uncultured strains from marine sediments. As a result, 238 strains belonging to 10 phyla, 26 families and 82 species were successfully isolated. Some uncultured rare taxa within <i>Chlorobi</i> and <i>Kiritimatiellaeota</i> were successfully cultured<i>.</i> Amongst the newly isolated uncultured microbes, most genomes, e.g. bacteria, possess SCFA oxidative degradation genes, and these features might aid these microbes in better adapting to the culture media. A further resuscitation analysis of a viable but non-culturable (VBNC) <i>Marinilabiliales</i> strain verified that the addition of SCFAs could break the dormancy of <i>Marinilabiliales</i> in 5 days, and the growth curve test showed that the SCFAs could shorten the lag phase and increase the growth rate. Overall, this study provides new insights into SCFAs, which were first studied as resuscitation factors in uncultured marine bacteria. Thus, this study can help improve the utilisation and excavation of marine microbial resources, especially for the most-wanted or key players.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00187-w.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 3","pages":"400-414"},"PeriodicalIF":5.7,"publicationDate":"2023-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10112155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity, community structure, and quantity of eukaryotic phytoplankton revealed using 18S rRNA and plastid 16S rRNA genes and pigment markers: a case study of the Pearl River Estuary.","authors":"Shumin Xu, Guihao Li, Cui He, Yi Huang, Dan Yu, Huiwen Deng, Zhuyin Tong, Yichong Wang, Christine Dupuy, Bangqin Huang, Zhuo Shen, Jie Xu, Jun Gong","doi":"10.1007/s42995-023-00186-x","DOIUrl":"10.1007/s42995-023-00186-x","url":null,"abstract":"<p><p>Understanding consistencies and discrepancies in characterizing diversity and quantity of phytoplankton is essential for better modeling ecosystem change. In this study, eukaryotic phytoplankton in the Pearl River Estuary, South China Sea were investigated using nuclear 18S rRNA and plastid 16S or 23S rRNA genes and pigment analysis. It was found that 18S abundance poorly explained the variations in total chlorophyll <i>a</i> (Chl-<i>a</i>). However, the ratios of log-transformed 18S abundance to Chl-<i>a</i> in the major phytoplankton groups were generally environment dependent, suggesting that the ratio has potential as an indicator of the physiological state of phytoplankton. The richness of 18S-based operational taxonomic units was positively correlated with the richness of 16S-based amplicon sequence variants of the whole phytoplankton community, but insignificant or weak for individual phytoplankton groups. Overall, the 18S based, rather than the 16S based, community structure had a greater similarity to pigment-based estimations. Relative to the pigment data, the proportion of haptophytes in the 18S dataset, and diatoms and cryptophytes in the 16S dataset, were underestimated. This study highlights that 18S metabarcoding tends to reflect biomass-based community organization of eukaryotic phytoplankton. Because there were lower copy numbers of plastid 16S than 18S per genome, metabarcoding of 16S probably approximates cell abundance-based community organization. Changes in biomass organization of the pigment-based community were sensitive to environmental changes. Taken together, multiple methodologies are recommended to be applied to more accurately profile the diversity and community composition of phytoplankton in natural ecosystems.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00186-x.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 3","pages":"415-430"},"PeriodicalIF":5.8,"publicationDate":"2023-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10164513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanding the application range of the <i>κ</i>‑carrageenase OUC-FaKC16A when preparing oligosaccharides from <i>κ</i>-carrageenan and furcellaran.","authors":"Chengcheng Jiang,&nbsp;Francesco Secundo,&nbsp;Xiangzhao Mao","doi":"10.1007/s42995-023-00181-2","DOIUrl":"10.1007/s42995-023-00181-2","url":null,"abstract":"<p><p>Carrageenan oligosaccharides are important products that have demonstrated numerous bioactivities useful in the food, medicine, and cosmetics industries. However, the specific structure-function relationships of carrageenan oligosaccharides are not clearly described due to the deficiency of high specific carrageenases. Here, a truncated mutant OUC-FaKC16Q based on the reported <i>κ</i>-neocarratetrose (N<i>κ</i>4)-producing <i>κ</i>-carrageenase OUC-FaKC16A from <i>Flavobacterium algicola</i> was constructed and further studied. After truncating the C-terminal Por_Secre_tail (PorS) domain (responsible for substrate binding), the catalytic efficiency and temperature stability decreased to a certain extent. Surprisingly, this truncation also enabled OUC-FaKC16Q to hydrolyze N<i>κ</i>4 into <i>κ</i>-neocarrabiose (N<i>κ</i>2). The offset of Arg₂₆₅ residue in OUC-FaKC16Q may explain this change. Moreover, the high catalytic abilities, the main products, and the degradation modes of OUC-FaKC16A and OUC-FaKC16Q toward furcellaran were also demonstrated. Data suggested OUC-FaKC16A and OUC-FaKC16Q could hydrolyze furcellaran to produce mainly the desulfated oligosaccharides DA-G-(DA-G4S)₂ and DA-G-DA-G4S, respectively. As a result, the spectrum of products of <i>κ</i>-carrageenase OUC-FaKC16A has been fully expanded in this study, indicating its promising potential for application in the biomanufacturing of carrageenan oligosaccharides with specific structures.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00181-2.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 3","pages":"387-399"},"PeriodicalIF":5.7,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10110064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ocellatuspyrones A‒G, new antibacterial polypropionates from the Chinese mollusk <i>Placobranchus ocellatus</i>.","authors":"Song-Wei Li,&nbsp;Dan-Dan Yu,&nbsp;Ming-Zhi Su,&nbsp;Li-Gong Yao,&nbsp;Hong Wang,&nbsp;Xueting Liu,&nbsp;Yue-Wei Guo","doi":"10.1007/s42995-023-00179-w","DOIUrl":"10.1007/s42995-023-00179-w","url":null,"abstract":"<p><p>Marine invertebrates serve as rich sources of secondary metabolites with intriguing chemical diversities and a wide spectrum of biological activities. Particularly, marine shell-less sacoglossan mollusks have attracted much attentions due to the fact that mollusks apply complex metabolites as chemical defense agents against to their predators. With the purpose of discovering bioactive secondary metabolites to develop marine-derived medicines from the South China Sea, we have conducted a chemical study on the photosynthetic mollusk <i>Placobranchus ocellatus.</i> As a result, seven new <i>γ</i>-pyrone polypropionates, namely ( ±)-ocellatuspyrone A (<b>1</b>), ( ±)-ocellatuspyrone B (<b>2</b>), and ocellatuspyrones C-G (<b>5</b>, <b>9</b>-<b>12</b>), along with five known polypropionates, have been isolated and characterized from the South China Sea photosynthetic mollusk <i>Placobranchus ocellatus</i>. Extensive spectroscopic analysis, single crystal X-ray diffraction analysis, modified Mosher's method, ECD comparison, CD exciton chirality method, TDDFT-ECD calculation, and chemical conversion were used to determine the structures and absolute configurations of the new compounds and the stereochemistry of undefined known compounds <b>4</b>, <b>6</b> and <b>7</b>. All these isolated polypropionates were evaluated in bioassays for their biological activities, including antibacterial, neuroprotective effect, anti-inflammatory, PTP1B inhibitory, and antiviral activities. Compounds <b>7</b>, <b>8</b> and <b>11</b> were found for the first time to show antibacterial activity against fish pathogenic bacteria <i>Streptococcus parauberis</i> (the main pathogen causing fish streptococcal infections and acute death) with MIC values of 35.8, 34.2, and 37.4 μg/mL, respectively, which might be potential novel antibacterial agents for the treatment of fish infectious diseases.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00179-w.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 3","pages":"373-386"},"PeriodicalIF":5.7,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10112156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenomic resolution of Imparidentia (Mollusca: Bivalvia) diversification through mitochondrial genomes.","authors":"Yu Wang,&nbsp;Yi Yang,&nbsp;Lingfeng Kong,&nbsp;Takenori Sasaki,&nbsp;Qi Li","doi":"10.1007/s42995-023-00178-x","DOIUrl":"10.1007/s42995-023-00178-x","url":null,"abstract":"<p><p>Despite significant advances in the phylogenomics of bivalves over the past decade, the higher-level phylogeny of Imparidentia (a superorder of Heterodonta) remains elusive. Here, a total of five new mitochondrial sequences (<i>Chama asperella</i>, <i>Chama limbula</i>, <i>Chama dunkeri</i>, <i>Barnea manilensis</i> and <i>Ctena divergens</i>) was added to provide resolution in nodes that required additional study<i>.</i> Although the monophyly of Lucinida remains less clear, the results revealed the overall backbone of the Imparidentia tree and the monophyly of Imparidentia. Likewise, most relationships among the five major Imparidentia lineages-Lucinida, Cardiida, Adapedonta, Myida and Venerida-were addressed with a well-supported topology. Basal relationships of Imparidentia recovered Lucinidae as the sister group to all remaining imparidentian taxa. Thyasiridae is a sister group to other imparidentian bivalves (except Lucinidae species) which is split into Cardiida, Adapedonta and the divergent clade of Neoheterodontei. Neoheterodontei was comprised of Venerida and Myida, the former of which now also contains Chamidae as the sister group to all the remaining venerid taxa. Moreover, molecular divergence times were inferred by calibrating nine nodes in the Imparidentia tree of life by extinct taxa. The origin of these major clades ranged from Ordovician to Permian with the diversification through the Palaeozoic to Mesozoic. Overall, the results obtained in this study demonstrate a better-resolved Imparidentia phylogeny based on mitochondrial genomes.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00178-x.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 3","pages":"326-336"},"PeriodicalIF":5.7,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10110065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}