Egyptian Academic Journal of Biological Sciences E Medical Entomology and Parasitology最新文献_第7页

Extraction and Detection of Domestic Pig DNA from Sarcophaga carnaria Linnaeus and Chrysomya albiceps Wiedemann Larval Crops Fed on Deceased Pig Remains 以死猪为食的石棺和白色金蝇幼虫作物中家猪DNA的提取与检测

Egyptian Academic Journal of Biological Sciences E Medical Entomology and Parasitology Pub Date : 2019-06-01 DOI: 10.21608/EAJBSE.2019.44737

M. Hassan, A. Bream, Ahmed M. Galal El-Di, Mohammad R. K. Abdel-Samad, E. Metwaly

{"title":"Extraction and Detection of Domestic Pig DNA from Sarcophaga carnaria Linnaeus and Chrysomya albiceps Wiedemann Larval Crops Fed on Deceased Pig Remains","authors":"M. Hassan, A. Bream, Ahmed M. Galal El-Di, Mohammad R. K. Abdel-Samad, E. Metwaly","doi":"10.21608/EAJBSE.2019.44737","DOIUrl":"https://doi.org/10.21608/EAJBSE.2019.44737","url":null,"abstract":"In order to investigate the possibility of detecting host DNA isolated from larval crops and the effect of time elapse during digestion, the present study was carried out. Domestic pig cadaver was used as a case study model instead of real human corpse tissue by collecting of 3rd instar larvae post-feeding and starving during24, 48, 72, and 96 hours. The identification of DNA extracted from pig tissues and from Sarcophaga carnaria Linnaeus and Chrysomya albiceps Wiedemann larval crops fed on deceased pig remains were carried out using PCR. The detection of DNA bands in the larvae of the two dipteran species was carried out by using Gel electrophoresis. Results showed that the extracted DNA from larval crops of the two fly species was identical to that extracted directly from domestic pig decaying tissues. In addition, the PCR specifically amplified the domestic pig mtDNA cyt b gene with 203 bp amplicons. Results also showed that DNA could be clearly detected within approximately 72h after the removal of larvae from pig corpse, suggesting reliable consecration of insects as valuable evidence in crime scenes. These results are useful for further consideration of the estimation of postmortem intervals (PMI) by the aid of forensic entomology.","PeriodicalId":52578,"journal":{"name":"Egyptian Academic Journal of Biological Sciences E Medical Entomology and Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74923282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of Medical Flies along Wadi El-Rayan Protected Area Wadi El-Rayan保护区医疗蝇的分布

Egyptian Academic Journal of Biological Sciences E Medical Entomology and Parasitology Pub Date : 2019-06-01 DOI: 10.21608/eajbse.2019.54557

K. Hammad, T. Selim, Mhmoud S. Boraey

引用次数: 2

Field Application of Honeybees’ Endogenous Lactic Acid Bacteria for the Control of American Foulbrood Disease 蜜蜂内源乳酸菌在防治美洲臭蝇病中的应用

Egyptian Academic Journal of Biological Sciences E Medical Entomology and Parasitology Pub Date : 2019-06-01 DOI: 10.21608/eajbse.2019.36658

F. Mahmoud, Shireen A. M. Ma’moun, Rasha M. A. Farag, A. Shafai, A. Zeid

引用次数: 2

The mechanical transmission of human parasites by cockroaches in Ismailia Governorate, Egypt. 埃及伊斯梅利亚省蟑螂对人类寄生虫的机械传播。

Egyptian Academic Journal of Biological Sciences E Medical Entomology and Parasitology Pub Date : 2019-06-01 DOI: 10.21608/eajbse.2019.46516

Ghada M. Abdel-Gahny, Ahmed H. Aboghalia, S. Elbanna, M. F. Soliman

{"title":"The mechanical transmission of human parasites by cockroaches in Ismailia Governorate, Egypt.","authors":"Ghada M. Abdel-Gahny, Ahmed H. Aboghalia, S. Elbanna, M. F. Soliman","doi":"10.21608/eajbse.2019.46516","DOIUrl":"https://doi.org/10.21608/eajbse.2019.46516","url":null,"abstract":"The role of cockroaches in the mechanical transmission of human parasites in Ismailia Governorate was studied. A total 1135 cockroaches, belonging to two families and four species, were collected from seven different sites using sticky traps. The cockroaches were examined for the presence of parasites. The total number of parasites was six, The Helminths were: Ascaris sp., Trichuris sp., Hymenolepis sp., Entrobius sp, and Taenia sp. The protozoa was: Entamoeba histolitica. The total prevalence of parasites in body surface was 12.1%, in the gut was 13.2% and in whole insect was 13.5%. The highest prevalence was recorded at the summer in whole insect with 18.8% while the lowest prevalence was at the spring on the body surface with 3.5%. On the other hand, parasites were not recorded in winter. The highest prevalence was recorded with Periplaneta americana occurring in the whole insect with 15.6% while the lowest prevalence was recorded with Blattella germanica occurring on the body surface with 12.6%. On the other hand, parasites were not recorded with Blatta orientalis and Supella longipalpa. The prevalence of parasites recorded in cockroach’s sexes and stages. The highest prevalence was recorded with females occurring in the whole insect with 20%, followed with males occurring in the whole insect too with 10.3% and the lowest prevalence was recorded in nymphs with 3.2% in the gut. The present study further confirms that cockroaches play an important role as mechanical vectors of protozoan and helminth parasites. Hence, it is recommended that appropriate control measures must be taken particularly to make human dwellings as well as food preparation areas, including hospitals, hostels, schools and farms free of cockroaches.","PeriodicalId":52578,"journal":{"name":"Egyptian Academic Journal of Biological Sciences E Medical Entomology and Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89216255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new Report of Parasitic Nematodes (Thelastomatidae) in Egyptian Cockroaches, Polyphaga aegyptiaca (Dictyoptera: Polyphagidae) in Sharkia Governorate, Egypt. 埃及Sharkia省埃及蜚蠊中寄生线虫新报告(双翅目:多翅目:多翅目)。

Egyptian Academic Journal of Biological Sciences E Medical Entomology and Parasitology Pub Date : 2019-06-01 DOI: 10.21608/EAJBSE.2019.43251

E. Nasr, A. A. A. Hoot, H. Sharaf, Salam S. Teleb

{"title":"A new Report of Parasitic Nematodes (Thelastomatidae) in Egyptian Cockroaches, Polyphaga aegyptiaca (Dictyoptera: Polyphagidae) in Sharkia Governorate, Egypt.","authors":"E. Nasr, A. A. A. Hoot, H. Sharaf, Salam S. Teleb","doi":"10.21608/EAJBSE.2019.43251","DOIUrl":"https://doi.org/10.21608/EAJBSE.2019.43251","url":null,"abstract":"There are many reports about thelastomatid parasitic nematode isolated from different species of cockroaches in many countries, without any clear information about those nematodes that are associated with Polyphaga aegyptiaca until now, so we have examined the nematodes were recovered from P. aegyptiaca and collected from Sharkia Gov., Egypt. Hammerschmidtiella diesingi and Leidynema appendiculata were found with the high infection rates in P. aegyptiaca. The results indicated that the prevalence of cockroaches infected with H. diesingi alone or integrated with another species was (51%), similarly, L. appendiculata was (65%) from total cockroaches. The mean intensity of L. appendiculata was higher than H. diesingi the current of cockroaches. The gravid females of both H. diesingi and L. appendiculata were also higher than of any other stage of nematodes. Finally, using light and electron microscope, in order to identify and determine the morphological characteristics of both species of nematode. Results also indicated that SEM is a powerful tool to identify the morphological characteristics such as head, female cephalic region, vulva, tail, anus, male cloaca area and papillae.","PeriodicalId":52578,"journal":{"name":"Egyptian Academic Journal of Biological Sciences E Medical Entomology and Parasitology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86522648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Abundance of Forensic Insects on Dog and Rabbit Carcasses in Different Habitats and Developmental Stages of Chrysomya albiceps as a Forensic Indicator 不同生境和不同发育阶段白绒金蝇犬、兔尸体上法医昆虫丰度的鉴定指标

Egyptian Academic Journal of Biological Sciences E Medical Entomology and Parasitology Pub Date : 2019-06-01 DOI: 10.21608/EAJBSE.2019.38885

M. Zeariya, M. Kabadaia

引用次数: 4

Parasitological and Biochemicals studies on The Impact of Chitosan Nanoparticles on the course of Murine, Schistosomasis mansoni 壳聚糖纳米颗粒对小鼠曼氏血吸虫病病程影响的寄生虫学和生化研究

Egyptian Academic Journal of Biological Sciences E Medical Entomology and Parasitology Pub Date : 2019-06-01 DOI: 10.21608/eajbse.2019.70455

A. A, G. F., M. S., I. A, Hassan A.

引用次数: 0

Medico-Legal Forensic Entomology “Use of Insects in Death Investigations”- A Review 法医学法医昆虫学“昆虫在死亡调查中的应用”综述

Egyptian Academic Journal of Biological Sciences E Medical Entomology and Parasitology Pub Date : 2019-06-01 DOI: 10.21608/eajbse.2019.61865

M. Kenawy, Y. M. Abdel-Hamid

引用次数: 1

Insecticidal Properties of Three Plant Extracts Against Culex quinquefasciatus Say and Aedes aegypti Linnaeus 三种植物提取物对致倦库蚊和埃及伊蚊的杀虫性能研究

Egyptian Academic Journal of Biological Sciences E Medical Entomology and Parasitology Pub Date : 2019-06-01 DOI: 10.21608/eajbse.2019.60911

A. Khandagle

引用次数: 0

Molecular Differentiation of Haemonchus longistipes Railliet & Henry, 1909 (Nematoda: Trichostrongyloidea) of Female Morphotypes and Male Worms Infecting the Dromedary Camelus dromedarius Using RAPD Technique 利用RAPD技术对单峰骆驼雌雄虫的分子分化研究(线虫:毛线虫总目

Egyptian Academic Journal of Biological Sciences E Medical Entomology and Parasitology Pub Date : 2019-06-01 DOI: 10.21608/eajbse.2019.48766

Ayman Elsayed, A. Badawy, A. Abdel-Aziz, M. Sarhan

引用次数: 0