Klinichescheskaya Laboratornaya Diagnostika最新文献

{"title":"Molecular genetic tests GeneXpert MTB/RIF and Xpert MTB/RIF (Ultra) in the diagnosis of tuberculosis (review of literature).","authors":"E A Borodulina,&nbsp;V V Piskun,&nbsp;M V Uraksina,&nbsp;A T Shubina","doi":"10.51620/0869-2084-2022-67-9-544-549","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-9-544-549","url":null,"abstract":"<p><p>In recent tuberculosis years is the main cause of morbidity and death among patients with HIV infection. Modern diagnostics of tuberculosis includes mass screening of the population: digital fluorography from the age of 15 and immunodiagnostics in children and adolescents. Detection of mycobacterium tuberculosis by microscopy occurs in forms of tuberculosis with the decay of lung tissue. Such patients represent a high epidemic risk. To improve the verification of diagnosis in the practice of a phthisiologist, molecular genetic methods for the search for mycobacteria are increasingly used, based on the identification of specific fragments of the DNA chain in the diagnostic material. The most widely used method is the polymerase chain reaction (PCR), which is based on directed DNA amplification. The latest innovation is fully automated systems using cartridge technology GeneXpert. The advantages of GeneXpert are high sensitivity, speed (result in 2 hours), real-time PCR detection, exclusion of sample contamination. The technique of cartridge technology is constantly being improved, various cartridges are used on its platform, which not only detect M. tuberculosis, but also determine the sensitivity to anti-tuberculosis drugs - rifampicin (MTB / RIF cartridge) or several anti-TB drugs (MTB / XDR). Cartridges have been developed that are able to detect Mycobacterium tuberculosis (MBT) at an even lower concentration in the test material - MTB / RIF (Ultra). GeneXpert technology can be used to diagnose extrapulmonary tuberculosis by examining various biological materials, which are more effective in detecting tuberculosis in children and adolescents, in HIV-positive individuals.</p>","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"67 9","pages":"544-549"},"PeriodicalIF":0.0,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33464825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardness of antimicrobial discs by various manufacturers in the light of import substitution.","authors":"Lubov Viktorovna Domotenko,&nbsp;A Yu Mironov,&nbsp;I S Kosilova,&nbsp;A P Shepelin","doi":"10.51620/0869-2084-2022-67-9-550-556","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-9-550-556","url":null,"abstract":"<p><p>In connection with the introduction of economic sanctions against the Russian Federation by the United States, the European Union, Japan, and a number of other countries, import substitution is becoming one of the strategic objectives of the Russian economy The comparative assessment of standardness and effectiveness of six antimicrobial commercial producer-varying disks for clinical and test strains of microorganisms has been conducted. It is based on analyzing of the variation coefficient (Cv) of inhibition zone diameters, mean values, and diameter values ranges, which are indicators of reproducibility, accuracy and efficiency, respectively. Discs from BD BBL result in insignificant variations of inhibition zone diameters, providing accurate and reproducible data. Imported discs available on the national market from a friendly country produced by HiMedia Company are showed more variation and inferior in quality to BD BBL ones. There are satisfactory results for some domestic discs, but discs with imipenem, meropenem, ciprofloxacin and gentamicin are not standard enough to assure reproducible results. To have reliable data on antibiotic susceptibility of infectious agents, incoming and routine quality control of antimicrobial disks is needed as a compulsory procedure for every bacteriological laboratory.</p>","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"67 9","pages":"550-556"},"PeriodicalIF":0.0,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33464826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the influence of the analytical characteristics of the microbiological blood study in bacteremia in a multidisciplinary hospital.","authors":"A V Haliulin,&nbsp;A V Lyamin,&nbsp;O A Gusjakova,&nbsp;A V Kozlov,&nbsp;S S Lebedeva","doi":"10.51620/0869-2084-2022-67-9-511-518","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-9-511-518","url":null,"abstract":"<p><p>The culture method continues to be the \"gold\" standard for microbiological diagnosis of bloodstream infections. This is primarily due to the fact that the definition of the etiology of a generalized infectious process determines the etiotropic antibiotic therapy. To do this, it is necessary to conduct periodic microbiological monitoring of the prevailing microflora. To do this, in the present study, a retrospective analysis of the results of a microbiological blood test for sterility was performed in case of suspected bloodstream infections in a multidisciplinary hospital to assess the influence of analytical stage factors on the laboratory data obtained. Automatic hematological cultivators were used, identification was carried out based on the biochemical characteristics of microorganisms, as well as using time-of-flight mass spectrometry with matrix-activated laser desorption / ionization (MALDI-TOF MS). More than 10,000 research results were analyzed, the average microflora seeding rate was 15.1%. The analysis of the isolated microflora was carried out in 2 groups of positive results: at the beginning, the data obtained in the presence of growth in two vials at once were evaluated, then the positive results of blood cultures obtained in any one vial from a pair were studied. The predominance of gram-positive flora in the structure of microorganisms isolated from whole blood was revealed, the influence of cultivation conditions and the composition of thenutrient medium on the isolated flora was not found, however, a number of microorganisms, due to the specific characteristics of metabolism, were characterized by growth under strictly defined cultivation conditions. The presented study actualizes the need for constant microbiological monitoring in order to determine the prevailing hospital microflora, which can contribute to a timely response in order to limit the spread of highly virulent, aggressive, resistant strains of microorganisms leading to the development of generalized bloodstream infections.</p>","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"67 9","pages":"511-518"},"PeriodicalIF":0.0,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33465863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of the distribution of the antigens of Kell, Duffy, Kidd, MNS, Lewis, Lutheran blood group systems in blood donors in the Kirov region.","authors":"E V Butina,&nbsp;A V Yovdiy,&nbsp;E A Poponina,&nbsp;M V Smolnikova,&nbsp;F S Sherstnev,&nbsp;I V Paramonov","doi":"10.51620/0869-2084-2022-67-9-504-506","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-9-504-506","url":null,"abstract":"<p><p>The frequency of occurrence of antigens of the Kell (Kpa, Kpb), Kidd, Duffy, MNS and Lutheran systems in donors of the Kirov region corresponds to the distribution of antigens characteristic of white Europeans. Antigens K (Kell system) and Lea (Lewis system) are detected in the population of the region much less frequently, antigen Leb (Lewis system) - more often than in the population of Europe. The presence of a registry of donors typed according to a wide range of red blood antigens is a prerequisite for the immunohematological safety of blood transfusions.</p>","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"67 9","pages":"504-506"},"PeriodicalIF":0.0,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33465861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hondrosarcoma of the chest with intraabdominal distribution (case report).","authors":"Svetlana Valerievna Smetanina,&nbsp;E N Slavnova,&nbsp;E Yu Uskova,&nbsp;A A Khusiyanova,&nbsp;V A Derzhavin","doi":"10.51620/0869-2084-2022-67-9-507-510","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-9-507-510","url":null,"abstract":"<p><p>A rare clinical observation of chondrosarcoma metastasis into the abdominal cavity with a specific metastatic ascitic fluid is presented. Chondrosarcomas that occur as a result of malignant transformation of benign chondroma are quite rare. Even less often in the literature, cases of chondrosarcoma metastases are described, especially in a peritoneum with the presence of metastatic ascites. The article describes a case of metastatic ascites in a 38-year old patient with chondrosarcoma, which developed against the background of previously resected rib chords. The article describes the cytological signs of chondrosarcoma in ascitic fluid.</p>","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"67 9","pages":"507-510"},"PeriodicalIF":0.0,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33465862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative method for the determination of antibiotic-resistant gut bacterial strains for the early diagnosis of chronic arthritis.","authors":"O E Chelpachenko,&nbsp;E I Danilova,&nbsp;N B Perunova,&nbsp;E V Ivanova,&nbsp;I A Nikiforov,&nbsp;I N Chainikova,&nbsp;Anastasia Vladimirovna Bekpergenova,&nbsp;T A Bondarenko","doi":"10.51620/0869-2084-2022-67-9-525-529","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-9-525-529","url":null,"abstract":"<p><p>Based on the clinical and microbiological monitoring of two groups of children aged 3 to 17 years with acute (n=78) and chronic (n=46) course of reactive arthritis (ReA), a method for early diagnosis of chronic arthritis was developed by determining the number of antibiotic-resistant coprostrains in patients with ReA, characterized by the absence of the need to isolate a pure culture of the pathogen and its identification; inoculation of faeces at a dilution of 10-5 on solid 1.5% GRM-agar with an antibacterial agents used in the treatment of a particular patient, at a minimum inhibitory concentration in the resistance range, followed by incubation and counting of the colonies of microorganisms grown on the plate. A significant relationship between the number of antibiotic-resistant gut bacterial strains and the course of arthritis (acute, chronic) was revealed, and the borderline value of the number of antibiotic-resistant gut bacterial strains was determined - 5×103 CFU/g, which allows differentiating the acute course from the chronic one: in the acute course< 5×103 CFU/g, with chronic - ≥ 5×103 CFU/g. The method allows, at the stage of completion of anti-inflammatory therapy in the active phase of the disease, to identify a risk group for the development of a chronic course of arthritis among patients with ReA, which can contribute to timely therapeutic measures aimed at preventing recurrence of the disease and making the patient disabled.</p>","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"67 9","pages":"525-529"},"PeriodicalIF":0.0,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33465865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospects for the use of blood elemental status to assess the molecular genetic profile of gliomas.","authors":"Larisa Mikhailovna Obukhova,&nbsp;I I Evdokimov,&nbsp;I A Medyanik,&nbsp;N Yu Orlinskaya,&nbsp;A S Grishin,&nbsp;V E Babintsev,&nbsp;Yu A Barinov,&nbsp;M M Kontorshchikov,&nbsp;T N Gorshkova,&nbsp;V F Lazukin","doi":"10.51620/0869-2084-2022-67-9-497-503","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-9-497-503","url":null,"abstract":"<p><p>It has been established that blood element homeostasis is related to gliomagenesis which increases the attractiveness of the analysis of its components as a promising preoperative mediated characteristics of the molecular genetic profile of gliomas. The aim of this work is to analyze the relationship between mineral metabolism parameters and immunohistochemical characteristics of glial tumors and evaluate the clinical significance of blood element homeostasis analysis for preoperative assessment of the molecular profile of gliomas. The levels of cancer specific markers MGMT, Ki-67, p-53, IDH1 were determined immunohistochemically using the corresponding antibody clones. Micronutrient levels were analyzed by inductively coupled plasma atomic emission spectrometry recalculating the results per 1 g of protein which was determined by the Lowry method. The data on cancer-specific marker levels obtained in primary brain tumors (20) and in blood plasma of gliomas patients (20) and practically healthy subjects (5) were compared using a number of statistical programs. We found significant differences in the levels of sodium, potassium, zinc and copper depending on the value of the mitotic index Ki-67 and IDH1 isocitrate dehydrogenase gene mutation. For the first time, a significant correlation showing the consistency between the level of glial tumor cancer-specific markers and blood mineral metabolism was observed. The revealed correlations provide new insights into understanding of gliomagenesis mechanisms and can be used as a predictive preoperative assessment of molecular genetic markers of gliomas.</p>","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"67 9","pages":"497-503"},"PeriodicalIF":0.0,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33465860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Educational video for training specialists in clinical laboratory diagnostics.","authors":"N B Zakharova,&nbsp;M A Godkov,&nbsp;V V Dolgov,&nbsp;V L Emanuel,&nbsp;A Zh Gilmanov,&nbsp;G P Gladilin","doi":"10.51620/0869-2084-2022-67-8-489-492","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-8-489-492","url":null,"abstract":"<p><p>The adaptation of educational programs under restrictions during the SARS-CoV-2 coronavirus pandemic and after it is based not only on the widespread use of video lectures, but also on the introduction of a number of new educational technologies. Hybrid learning will become the cornerstone of future educational technologies in clinical laboratory diagnostics and will contribute to the creation of virtual practical classes with examples of analysis of laboratory testing results based on case histories (Case Technologies). The key aspect in the implementation of video cases into the educational process is the development of video materials. The use of educational video cases developed by Vector-Best in the process of training specialists in clinical laboratory diagnostics during the cycles «Retraining» and «General Improvement» at the Department of Clinical Laboratory Diagnostics of the Saratov State Medical University named after V. I. Razumovsky of the Ministry of Health of Russia was a convenient format and received positive assessment of cadets. Currently, the «IVD gallery» section has appeared on the FLM website and the placement of an additional educational resource - a library of educational video cases.</p>","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"67 8","pages":"489-492"},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33463086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromogranin B in blood serum of patients with neuroendocrine tumors.","authors":"Nina Vasilyevna Lyubimova,&nbsp;Yu S Timofeev,&nbsp;A A Markovich,&nbsp;A V Lebedeva,&nbsp;E I Karamisheva,&nbsp;V V Delektorskaya,&nbsp;N E Kushlinskii","doi":"10.51620/0869-2084-2022-67-8-440-443","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-8-440-443","url":null,"abstract":"<p><p>Neuroendocrine tumors (NETs) are a heterogeneous group of neoplasms from cells of the diffuse neuroendocrine system. Chromogranin B (CgB) is an acidic protein of the granin family, which can be used to detect the tumours of neuroendocrine nature. Analysis of levels and evaluation of the diagnostic efficiency of CgB in the blood serum of patients with NETs of various localizations. Patients with NETs (n=121) without specific treatment were examined. In the study were presented next localizations: 74 - pancreas, 20 - stomach, 12 - large intestine, 15 - other localizations (lungs, mammary gland, prostate gland, NETs with unidentified primary). 54 practically healthy donors were examined as control group. The determination of CgB in blood serum was performed with ELISA method on BEP 2000 analyzer using a standardized test system Human Chromogranin B (USCN, China). CgB levels in common NET group (median 18.9 ng/mL) were statistically significantly higher than in the control group (8.8 ng/mL). The highest median was obtained in group of intestinal NETs (21.2 ng/ml), which exceeded the median of the control group by more than 2.4 times. According to ROC analysis in the common NET group relative to the control group, the area under the curve AUC was 0.88 (95% CI 0.83-0.929). According to cut-off level of CgB - 15.8 ng/ml, the diagnostic sensitivity was 69.4%, with a specificity of 96.3%. The highest diagnostic sensitivity was in the group of the intestinal NETs (75.0%) and pancreas (71.2%). The study showed the significance of CgB as a potential biochemical marker of NETs with various localizations, alternative to CgA.</p>","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"67 8","pages":"440-443"},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33462628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of marker periodontopathogenic bacteria with the immune component sCD₁₄ secretion level in inflammatory periodontal diseases.","authors":"Nataliya Valerievna Davidovich,&nbsp;A S Galieva,&nbsp;A S Opravin,&nbsp;T Yu Gagarina,&nbsp;O G Malygina,&nbsp;S N Leikhter,&nbsp;E N Bashilova,&nbsp;T A Bazhukova","doi":"10.51620/0869-2084-2022-67-8-471-475","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-8-471-475","url":null,"abstract":"<p><p>Lipopolysaccharide of the cell wall of gram-negative bacteria is a highly active biological substance: its interaction with toll-like receptors-4 (TLR-4) of myeloid cells leads to the activation of a cascade of inflammatory reactions, which is accompanied by the release of the soluble CD14 receptor (sCD14), which can be considered not only as a marker of cell activation by endotoxin, but also as a marker of microbial translocation. The aim of the work was to assess the prognostic significance of the sCD14 level in the samples of the periodontal pocket in inflammatory periodontal diseases and the relationship of its secretion with marker periodontopathogens. For the study, washes were obtained from the periodontal pocket (88 samples in total) from patients with chronic periodontitis and intact periodontium. The sCD14 content was determined by ELISA; during real-time PCR, the marker periodontopathogens Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Treponema denticola, Porphyromonas gingivalis, Prevotella intermedia, and Candida albicans were isolated. The study revealed differences in the level of sCD14 secretion by groups: in chronic periodontitis, its content was 8,5 times higher than in the control group and amounted to 17,2±4,06 ng/ml (p=0,006). The frequency of detecting genes of periodontal pathogenic bacteria was 89,3% in patients with periodontitis and 31,25% in the group with intact periodontium. An interesting dependence of the detection of periodontal pathogenic bacteria in the group of patients with chronic periodontitis was established depending on the content of sCD14. Thus, at high concentrations of soluble coreceptor, a greater number of periodontopathogenic bacteria of the I and II orders were released. Thus, in inflammatory periodontal diseases, the processes of sCD14 synthesis change, which is probably due to the colonization of periodontal pathogenic bacteria and the action of their toxins and aggression factors. The relationship of marker periodontopathogens with the level of secretion of the immune component sCD14 and its effect on the structure of the periodontal index reflect shifts in the processes of reparative regeneration of the oral mucosa and the regulation of local immunity in response to microbial invasion.</p>","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"67 8","pages":"471-475"},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33462633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}