Journal of Biomedical Photonics and Engineering最新文献

{"title":"Characterization of Polarization Holographic Gratings Obtained on Azopolymer Thin Films by Digital Holographic Microscopy","authors":"V. Cazac, C. Loshmanschii, E. Achimova, A. Meshalkin, V. Abashkin, V. Podlipnov","doi":"10.18287/jbpe21.07.030306","DOIUrl":"https://doi.org/10.18287/jbpe21.07.030306","url":null,"abstract":". Polarization diffraction gratings are formed by one-step polarization holographic recording in azopolymer thin films. The evolution of the gratings parameters, such as the modulation of diffraction efficiency and relief depth with regard to different exposure dose is analyzed. Phase-shifting digital holographic microscopy is applied for the measurement of the light-induced polarization diffraction gratings. For the accurate hologram acquisition and reconstruction of the complex amplitude transmitted by the gratings, we performed all-optical (without moving components) phase-shifting implemented within in the imaging system of the digital holographic microscope. The experimental measurement results and theoretical predictions were compared and analyzed. © 2021 Journal of Biomedical Photonics & Engineering.","PeriodicalId":52398,"journal":{"name":"Journal of Biomedical Photonics and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48835788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Registration of Regeneration in Planarians from Photographic Images","authors":"K. Tiras, L. Mestetsky, S. Nefedova, N. Lomov","doi":"10.18287/jbpe21.07.030303","DOIUrl":"https://doi.org/10.18287/jbpe21.07.030303","url":null,"abstract":". In this study, an approach to constructing a mathematical model for quantifying the dynamics of regeneration of planarian flatworms in biological experiments is considered, based on an analysis of a series of digital microscopic images. A method is proposed to describe the body shape of a planarian using a continuous morphological model, based on the concept of a medial representation of the worm’s silhouette. The silhouette in this case is a polygon approximating the contours of the planarian’s body. The medial representation of the figure includes a medial axis and a radial function that describes the width of the figure relative to the medial axis. We propose a set of morphological criteria for assessing the dynamics of regeneration based on a continuous morphological model and present the results of computational experiments. © 2021 Journal of Biomedical Photonics & Engineering.","PeriodicalId":52398,"journal":{"name":"Journal of Biomedical Photonics and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46242155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Method for Assessing the Pupil Center Coordinates in Eyetracking with a Free Head Position","authors":"Gennadiy I. Gromilin, N. S. Yakovenko","doi":"10.18287/jbpe21.07.030302","DOIUrl":"https://doi.org/10.18287/jbpe21.07.030302","url":null,"abstract":". IR-illuminated Eyetracking systems include cornea reflection and pupil center coordinates detection to calculate the ope rator’ s gaze fixation point. When you turn a view of a large angle, some of the frames are blurred, and the coordinates are unreliable. The article describes a method for determining the center of the pupil in the gaze fixation system for operation at an increased camera frame rate. Comparison with known algorithms is given. The algorithm execution average time is about 1.2 ms on a typical office computer by processing images in fragments of the order of 340x240 pixels. © 2021 Journal of Biomedical Photonics & Engineering.","PeriodicalId":52398,"journal":{"name":"Journal of Biomedical Photonics and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43460445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Numerical Modeling of Radiation Focusing by Dielectric Microcylinders with Several Layers","authors":"A. Savelyeva, E. Kozlova","doi":"10.18287/jbpe21.07.030304","DOIUrl":"https://doi.org/10.18287/jbpe21.07.030304","url":null,"abstract":"In this paper, the light focusing by dielectric microcylinders with several layers was numerically studied by using the finite element method implemented in COMSOL Multiphysics. Different materials for the top layer were proposed during the research (refractive indices are 1.59, 1.8, 1.9) while the core was proposed from fused silica glass (refractive index is 1.45). It was shown that the dielectric shell allows to increases the maximum intensity of the formed photonic nanojet. Choosing the design of a two-layered microcylinder it is possible to form a compact focal spot (TE-polarization) or increase the length of the produced photonic nanojet. Obtained results were verified by another software package FullWAVE which implements a finite difference time domain method. The simulation results are in good agreement. © 2021 Journal of Biomedical Photonics & Engineering.","PeriodicalId":52398,"journal":{"name":"Journal of Biomedical Photonics and Engineering","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41442673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phthalocyanines Structure Versus Photodynamic Effectiveness towards Pathogenic Microorganisms: Our Recent Experience","authors":"V. Mantareva, I. Angelov, Meliha Syuleyman, V. Kussovski, I. Eneva, L. Avramov, E. Borisova","doi":"10.18287/jbpe21.07.040202","DOIUrl":"https://doi.org/10.18287/jbpe21.07.040202","url":null,"abstract":". The present review paper aims to summarize our recent experience in research and development of new phthalocyanine complexes and investigations of the main photophysical, photochemical and photobiological properties which are related to antimicrobial photodynamic therapy (aPDT) as alternative method for inactivation of the resistant pathogens. The effect of functionalization of Zn(II) phthalocyanine (ZnPc) with biologically-active natural substances such as amino acids, sugars and steroids was studied in comparison to the basic ZnPc ring molecule. The structural features of the substitution groups were chosen to facilitate the main properties responsible for PDT outcome. For example, the linkage groups of amino acids tyrosine, phenylalanine, lysine and arginine have positive charge in physiological media to the better attachment to bacterial wall and some of them have a good fluorescence for a contribution to the visualization of the infected area. Also, ZnPcs linked to sugars and steroids was expecting to possess receptor specific selectivity. The physicochemical properties of the novel functionalized ZnPcs are presented in respect to their efficiency for a number of pathogenic bacterial and fungal species. Additionally, the complexes of two heavy metal ions such as lutetium(III) (Lu(III)) and tin(IV) (Sn(IV)) were synthesized and evaluated for antimicrobial PDT. These complexes were designed with the same structural skeleton as our previous water-soluble methylpiridyloxy-substituted phthalocyanine complexes with zinc (II), silicon (IV), germanium (IV), indium (III) and gallium (III), all with relatively promising antibacterial efficiency. © 2021 Journal of Biomedical Photonics & Engineering.","PeriodicalId":52398,"journal":{"name":"Journal of Biomedical Photonics and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48973584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accuracy of Common Motion Estimators in Wave-Based Optical Coherence Elastography","authors":"Justin R. Rippy, Manmohan Singh, S. Aglyamov, K. Larin","doi":"10.18287/jbpe21.07.040303","DOIUrl":"https://doi.org/10.18287/jbpe21.07.040303","url":null,"abstract":"Motion estimators are commonly used in shear wave optical coherence elastography to compute small displacements. This work focuses on comparing three motion estimators: Kasai, Loupas, and the vector method. Our results show that the vector method is superior in low-SNR, low-amplitude situations for group velocity calculation and profile reconstruction. © 2021 Journal of Biomedical Photonics & Engineering.","PeriodicalId":52398,"journal":{"name":"Journal of Biomedical Photonics and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47897174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue Spectroscopy and Optical Clearing of Colorectal Mucosa in the Pursuit of New Cancer Diagnostic Approaches","authors":"Luís Fernandes, H. Silva, Inês H Martins, S. Carvalho, I. Carneiro, R. Henrique, V. Tuchin, Luís Manuel Couto Oliveira","doi":"10.18287/JBPE21.07.040302","DOIUrl":"https://doi.org/10.18287/JBPE21.07.040302","url":null,"abstract":"In this paper we present three studies that demonstrate the applicability of spectroscopy methods and optical clearing treatments in pathology identification and monitoring. In the first study, by obtaining the absorption spectra of human healthy and pathological (adenocarcinoma) colorectal mucosa tissues, it was possible to identify a higher content of a pigment in the diseased tissues. This study also shows that machine learning methods can be used to reach the same differentiated results  in vivo  through diffuse reflectance spectroscopy. In the second study, the combination of collimated transmittance spectroscopy with optical clearing treatments allowed to obtain the diffusion coefficients of glucose in healthy and pathological colorectal mucosa as:  D glucose =5.8x 10 – 7  cm 2 /s and  D glucose =4.4x 10 – 7  cm 2 /s, respectively. This study also demonstrated that the diseased tissues contains about 5% more mobile water than the healthy tissues. The third study was performed to evaluate the protein dissociation mechanism of optical clearing. By treating both healthy and pathological colorectal mucosa tissues with 93%-glycerol, a protein dissociation rate of about 3 times higher was obtained for the pathological mucosa. All the discriminating parameters that result from these studies can be obtained in the  in vivo  situation through diffuse reflectance spectroscopy and further studies to evaluate their values in different stages of cancer progression are of great importance to develop disease monitoring protocols.","PeriodicalId":52398,"journal":{"name":"Journal of Biomedical Photonics and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47205091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review of EEG Signal Analysis for Diagnosis of Neurological Disorders using Machine Learning","authors":"Vandana Joshi, Nirali R. Nanavati","doi":"10.18287/10.18287/JBPE21.07.040201","DOIUrl":"https://doi.org/10.18287/10.18287/JBPE21.07.040201","url":null,"abstract":"Neurological disorders are diseases that affect the brain and the central autonomic nervous systems. These disorders take a huge toll on an individual's health and general well-being. After cardiovascular diseases, neurological disorders are the main cause of death. These disorders include epilepsy, Alzheimer ’ s disease, dementia, cerebrovascular diseases including stroke, migraine, Parkinso n ’s disease and numerous other disorders. This manuscript presents a state-of-the-art consolidated review of research on the diagnosis of the three most common neurological disorders using electroencephalogram (EEG) signals with machine learning techniques. The disorders discussed in this manuscript are the more prevalent disorders  like  epilepsy, Attention-deficit/hyperactivity disorder (ADHD), and Alzheimer’s disease. This manuscript helps in understanding the details about EEG signal processing for diagnosis and analysis of neurological disorders along with a discussion of the datasets, limitations, results and research scope of the various techniques.","PeriodicalId":52398,"journal":{"name":"Journal of Biomedical Photonics and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48807425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photodynamic Action in Thin Sensitized Layers: Estimating the Utilization of Light Energy","authors":"G. Meerovich, I. Romanishkin, E. Akhlyustina, M. Strakhovskaya, E. Kogan, I. Angelov, V. Loschenov, E. Borisova","doi":"10.18287/jbpe21.07.040301","DOIUrl":"https://doi.org/10.18287/jbpe21.07.040301","url":null,"abstract":". The result of photodynamic action significantly depends on the density of the light dose absorbed by the photosensitizer. The efficiency of using light to excite photosensitizer molecules and minimization of its loss plays an important role in ensuring the overall success of the process. When carrying out photodynamic treatment of thin sensitized layers (such as inactivation of surface pathogens or in vitro screening studies of photosensitizers), only a part of the light dose is absorbed in the layer, while a significant part is lost, especially at low concentrations of the photosensitizer. In this work, we evaluate the decrease in absorbed light dose depending on the extinction and concentration of the photosensitizer in a thin sensitized layer, the shape of its absorption spectrum, and the shape of the excitation light source spectrum. It was found out that a significant loss of the absorbed dose occurs upon excitation of photosensitizers, especially with low extinction, when using light sources with a broad emission spectrum. This loss must be taken into consideration when predicting the results of photodynamic exposure and optimizing its tactics. © 2021 Journal of Biomedical Photonics & Engineering.","PeriodicalId":52398,"journal":{"name":"Journal of Biomedical Photonics and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44385584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Sedimentation Rate of Free Erythrocytes and Their Associates, the Influence of a Standing Ultrasonic Wave","authors":"V. Doubrovski, S. V. Markov, S. O. Torbin, E. P. Karpocheva","doi":"10.18287/JBPE21.07.040501","DOIUrl":"https://doi.org/10.18287/JBPE21.07.040501","url":null,"abstract":"A comparative analysis of the sedimentation rates of free erythrocytes and their associates – aggregates and agglutinates was carried out. The influence of a standing ultrasonic (US) wave on the blood sedimentation process was investigated. The problem was dictated not only by a purely scientific interest in the sedimentation of micro–objects in a liquid medium, but from a practical point of view also – the development of an instrumental acousto–optical method for human blood group typing. Registration of the blood sedimentation process was carried out by means of digital video recording with subsequent computer processing of the photographic images obtained. The magnitude of blood sedimentation rate was experimentally determined for various samples and conditions: 1) blood solutions with / without ultrasound exposure for different time dosages; 2) a mixture of blood samples with hemagglutinating substances (Tsoliklon) without / with ultrasonic action both for a positive agglutination reaction (formation of agglutinates) and negative ones (absence of agglutinates). Two approaches were used to process the photographic images: 1) in the case of agglutinates sedimentation the discrete counting and analysis of agglutinate parameters were used; 2) in other cases, the blood sedimentation rate was determined by the movement of the \"supernatant – sediment\" (plasma solution – erythrocytes mass) boundary. For the experimental conditions accepted the blood sedimentation rates were compared and, correspondently, the influence of the ultrasonic standing wave upon the process under study was estimated. It was shown that in the presence of ultrasound the sedimentation rate of RBC agglutinates (positive agglutination reaction) is more than an order higher than the sedimentation rate of free erythrocytes and their aggregates (negative agglutination reaction). Such a great difference in the sedimentation rates of agglutinates in comparison with free erythrocytes and their aggregates makes it possible to obtain a high degree of resolution of acousto–optical method for instrumental blood typing, and, hence, high reliability in blood group determination.","PeriodicalId":52398,"journal":{"name":"Journal of Biomedical Photonics and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48909618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}