Journal of Biomedical Photonics and Engineering最新文献_第2页

Laser-Induced Crystallization of Standard Proteins on Ultra-Hydrophobic Surface and Characterization Using Raman Spectroscopy 超疏水表面标准蛋白的激光诱导结晶及拉曼光谱表征

Journal of Biomedical Photonics and Engineering Pub Date : 2023-09-04 DOI: 10.18287/jbpe23.09.030312

B. Sudarshan Acharya, Sajan D. George, Abdul Ajees Abdul Salam

{"title":"Laser-Induced Crystallization of Standard Proteins on Ultra-Hydrophobic Surface and Characterization Using Raman Spectroscopy","authors":"B. Sudarshan Acharya, Sajan D. George, Abdul Ajees Abdul Salam","doi":"10.18287/jbpe23.09.030312","DOIUrl":"https://doi.org/10.18287/jbpe23.09.030312","url":null,"abstract":"Structural information helps to understand the function of the proteins and provides potential protein-ligand interactions of new drugs. X-ray crystallography is a powerful technique to determine the structure in three-dimensional geometry. However, obtaining high-quality single crystals remains an obstacle in macromolecular crystallography. Laser-induced crystallization is emerging as an alternative technique to circumvent this problem. In this study, we have prepared ultra-hydrophobic surfaces and used them for protein crystallization. Three model proteins, lysozyme, ferritin, and proteinase K, with distinct hydrophobicity, were used for this study. The protein droplet placed on three surfaces (non-siliconized, siliconized, and candle soot films) is exposed to a diode laser (785 nm, 75 mW). Ultra-hydrophobic candle soot surfaced coverslips rapidly yielded the crystals in conventional and laser-exposed droplets. Proteinase K nucleated faster than the lysozyme/ferritin on candle soot coated surface, compared to the regular coverslips suggesting that ultra-hydrophobic surfaces assisted laser-induced crystallization will play an essential role in protein crystallization.","PeriodicalId":52398,"journal":{"name":"Journal of Biomedical Photonics and Engineering","volume":"336 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135454007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silver Anchored α-MnO2 Nanorods Based SERS Substrates for Salivary Thiocyanate Detection and Application in Oral Cancer Diagnosis 基于银锚定α-MnO2纳米棒的SERS底物唾液硫氰酸盐检测及其在口腔癌诊断中的应用

Journal of Biomedical Photonics and Engineering Pub Date : 2023-08-31 DOI: 10.18287/jbpe23.09.030311

Navami Sunil, Rajesh Unnathpadi, Biji Pullithadathil

引用次数: 0

Concentration Dependent Thermal Diffusivity of Mn3O4 Nanoparticles Using Dual Beam Thermal Lens Technique 利用双光束热透镜技术研究Mn3O4纳米颗粒的热扩散系数

Journal of Biomedical Photonics and Engineering Pub Date : 2023-08-28 DOI: 10.18287/jbpe23.09.030310

Jayaprasad K. V., Titu Thomas, Manu Vaishakh, Sheenu Thomas

引用次数: 0

A review of career devoted to Biophotonics – In memoriam to Ekaterina Borisova (1978–2021) 生物光子学职业回顾——纪念叶卡捷琳娜·鲍里索娃（1978–2021）

Journal of Biomedical Photonics and Engineering Pub Date : 2021-12-01 DOI: 10.18287/jbpe21.07.040101

Tsanislava Genova, L. Avramov, B. Kolev, A. Gisbrecht, I. Bliznakova, Lidiya Zaharieva, V. Mircheva, S. Ilyov, I. Angelov, V. Mantareva, P. Troyanova, P. Pavlova, T. Novikova, R. Ossikovski, D. Ivanov, V. Dremin, E. Rafailov, Sergey G. Sokolovsky, A. Bykov, I. Meglinski, I. Bratchenko, O. Semyachkina-Glushkovskaya, E. Genina, A. Bashkatov, A. Bucharskaya, V. Zakharov, J. Spigulis, Sindhoora Kaniyala Melanthota, S. K U, N. Mazumder, Shama Prasad K., P. Townsend, Luís Manuel Couto Oliveira, A. Priezzhev, D. Sterenborg, V. Tuchin

引用次数: 0

Devices for Photodynamic Studies Based on Light-Emitting Diodes 基于发光二极管的光动力学研究装置

Journal of Biomedical Photonics and Engineering Pub Date : 2021-11-11 DOI: 10.18287/jbpe21.07.040308

G. Meerovich, K. Linkov, A. V. Nekhoroshev, A. Borodkin, E. Akhlyustina, I. Angelov, D. Glechik, V. Loschenov

引用次数: 4

Activation of Apoptosis and Autophagy by Gratiola Officinalis Extract in Human Tumor Cell Lines Gratila Officinalis提取物对人肿瘤细胞凋亡和自噬的激活作用

Journal of Biomedical Photonics and Engineering Pub Date : 2021-11-10 DOI: 10.18287/jbpe21.07.040307

N. Polukonova, M. Baryshnikova, Dmitry A. Khochankov, E. V. Stepanova, E. Solomko, A. Polukonova, D. A. Mudrak, A. M. Mylnikov, A. Bucharskaya, G. Maslyakova, N. Navolokin

{"title":"Activation of Apoptosis and Autophagy by Gratiola Officinalis Extract in Human Tumor Cell Lines","authors":"N. Polukonova, M. Baryshnikova, Dmitry A. Khochankov, E. V. Stepanova, E. Solomko, A. Polukonova, D. A. Mudrak, A. M. Mylnikov, A. Bucharskaya, G. Maslyakova, N. Navolokin","doi":"10.18287/jbpe21.07.040307","DOIUrl":"https://doi.org/10.18287/jbpe21.07.040307","url":null,"abstract":". The problem of creating antitumor drugs with new mechanisms of action that predominantly induce apoptosis is still topical. The extract of Gratiola officinalis is a potential antitumor agent containing mainly flavonoids. The aim of this research is to study the effects of Gratiola officinalis extract on activation of apoptosis and autophagy in breast adenocarcinoma SK-BR-3 and kidney carcinoma A-498 lines. Apoptotic activity of the extract was studied by flow cytofluorometry using Hoechst stain and double staining with annexin V plus propidium iodide. There was 96.3% of cells in SK-BR-3 culture in late apoptosis phase detected by flow cytofluorometry method at the extract concentration of 0.88 mg/ml, 86.3% of cells were in apoptosis by Hoechst stain. The concentration of 0.82 mg/ml caused apoptosis in half of the cells. The extract has cytoprotective activity at low concentration (0.0352 mg/ml). The cytoprotection mechanism is realized through the activation of autophagy. The maximum number of autophagosomes in kidney carcinoma cells is observed at the extract concentration of 0.056 mg/ml. Thus, Gratiola officinalis extract is able to block cytoprotective autophagy with increasing the extract concentration and to activate apoptosis in 85% of tumor cells. Detailed research should be continued to understand the mechanisms of antitumor activity of Gratiola officinalis extract.","PeriodicalId":52398,"journal":{"name":"Journal of Biomedical Photonics and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46564036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Melanin Content on Laser Doppler Flowmetry and Tissue Reflectance Oximetry Signal Formation 黑色素含量对激光多普勒血流仪和组织反射血氧仪信号形成的影响

Journal of Biomedical Photonics and Engineering Pub Date : 2021-11-05 DOI: 10.18287/jbpe21.07.040306

V. Dremin, N. Golubova, E. Potapova, A. Dunaev

引用次数: 0

Laser Applications for Estimation of Microbial Activity and Investigation of NIR Skin Autofluorescence 激光在微生物活性评估中的应用及近红外皮肤自身荧光的研究

Journal of Biomedical Photonics and Engineering Pub Date : 2021-11-04 DOI: 10.18287/jbpe21.07.040305

A. Lihachev, D. Bļizņuks, J. Liepiņš, I. Lihacova

引用次数: 0

Visualization and Spectral Analysis of Pubic Periosteum Atrophy 耻骨骨膜萎缩的可视化及频谱分析

Journal of Biomedical Photonics and Engineering Pub Date : 2021-11-02 DOI: 10.18287/jbpe21.07.040502

V. Belokonev, V. Zakharov, S. Pushkin, Z. Kovaleva, Andrey V. Zharov, N. S. Burnaeva, E. Zakharova, Y. Khristoforova

引用次数: 0

Assessing Porcine Iris Elasticity and Mechanical Anisotropy with Optical Coherence Elastography 用光学相干弹性成像评价猪虹膜弹性和力学各向异性

Journal of Biomedical Photonics and Engineering Pub Date : 2021-10-29 DOI: 10.18287/jbpe21.07.040304

Christian Zevallos-Delgado, Taye Mekonnen, Fernando Zvietcovich, Manmohan Singh, S. Aglyamov, K. Larin

{"title":"Assessing Porcine Iris Elasticity and Mechanical Anisotropy with Optical Coherence Elastography","authors":"Christian Zevallos-Delgado, Taye Mekonnen, Fernando Zvietcovich, Manmohan Singh, S. Aglyamov, K. Larin","doi":"10.18287/jbpe21.07.040304","DOIUrl":"https://doi.org/10.18287/jbpe21.07.040304","url":null,"abstract":". The relaxation and contraction of the sphincter and dilator muscles of the iris play a critical role in vision, yet little is known about the biomechanical properties of these muscles. This study aimed to determine the elastic properties of the iris as a function of its anatomy and intraocular pressure. A high-resolution phase-sensitive OCE system was employed to detect acoustic radiation force induced propagation of elastic waves in the porcine iris in situ. Experiments were conducted at four different intraocular pressures (5, 10, 20, and 30 mmHg) with mechanical excitation at 1 kHz. We found that there was no significant difference in the wave speed at the different intraocular pressures. The results show that the stiffness of the iris was significantly higher in the semi-azimuthal orientation (mean wave speed of 2.5 m/s) than in the radial orientation (mean wave speed of 1.5 m/s). These measurements provide essential insights into the elastic properties of the iris, and they can be used for the characterization of eye conditions. © 2021 Journal of Biomedical Photonics & Engineering. non-natural modifications). These results suggest the potential use of","PeriodicalId":52398,"journal":{"name":"Journal of Biomedical Photonics and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45368687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4