Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology最新文献

{"title":"Apoptosis-inducing photodynamic therapy via dual-wavelength responsive ICG/Ce6 incorporated mesoporous silica nanoparticles in prostate cancer cells.","authors":"Emel Bakay, Ayşenur Pamukçu, Didem Sen Karaman, Nermin Topaloğlu","doi":"10.1007/s43630-025-00759-y","DOIUrl":"https://doi.org/10.1007/s43630-025-00759-y","url":null,"abstract":"<p><p>Photodynamic therapy (PDT) destroys cancer cells by photoactivation of photosensitive agents with minimal side effects on healthy tissue. Due to its limitations, using nanoparticles is a promising strategy to increase the efficacy of PDT. Mesoporous silica nanoparticles (MSN) are advantageous candidates for delivering the photosensitizer with their biocompatibility, pore structure, and large surface area. In this study, a novel design of MSN is produced by incorporating indocyanine green (ICG) and chlorin e6 (Ce6) into its structure. A dual-laser system is set up with 655- and 808-nm diode lasers for the PDT applications on prostate cancer cells (PC3). After the applications, the action mechanism is investigated via cell viability, cell staining, mechanistic, and qRT-PCR analyses. 100 μg/ml of nanoparticle concentration shows the highest anticancer PDT activity with a rate of 75% decrease in cell viability and exhibits minimal side-effect on healthy cells. Besides the overexpression of the Caspase 3 gene, all mechanistic and staining analyses support the apoptotic cell death mechanism of PDT in the presence of this nanoparticle. This study shows that PDT mediated by the novel design of MSN is a promising treatment for lethal cancer types and will enlighten future studies.</p>","PeriodicalId":520750,"journal":{"name":"Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144719374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements and emerging trends in photodynamic therapy: innovations in cancer treatment and beyond.","authors":"Mohamed Z El-Sadek, Mostafa K Abd El-Aziz, Ahmed H Shaaban, Salah Abdelfatah Mostafa, Al-Hassan Soliman Wadan","doi":"10.1007/s43630-025-00765-0","DOIUrl":"https://doi.org/10.1007/s43630-025-00765-0","url":null,"abstract":"<p><p>Photodynamic therapy (PDT) has emerged as a sophisticated, non-invasive therapeutic approach for treating various malignancies and non-oncological conditions. This review examines the fundamental principles and recent advances in PDT oncological applications. PDT's mechanism relies on the selective accumulation of photosensitizers (Ps) in pathological tissues followed by localized activation via specific wavelengths of light, generating cytotoxic species that induce tumor cell death while sparing adjacent healthy tissues. Despite its proven efficacy, conventional PDT faces limitations, including suboptimal Ps delivery, insufficient tissue penetration, and potential side effects. Recent breakthroughs in nanotechnology have created unprecedented opportunities to overcome these challenges through engineered nanocarriers that improve photosensitizer pharmacokinetics, biodistribution, and target specificity. This review explores multifunctional nanoformulations combining PDT with complementary therapeutic modalities such as photothermal therapy and chemotherapy. The review also addresses emerging trends in clinical translation, highlighting recent trials that demonstrate promising outcomes across multiple cancer types. We conclude by identifying remaining challenges and future directions for optimizing enhanced PDT as a precision anticancer strategy with the potential to impact standard treatment protocols for various malignancies significantly.</p>","PeriodicalId":520750,"journal":{"name":"Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144700973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling role of serum albumin in disaggregation and cellular delivery of a near-infrared chlorophyll-based photosensitizer in breast cancer cells.","authors":"Sucharita Chatterjee, Alok Dube, Shovan Kumar Majumder","doi":"10.1007/s43630-025-00764-1","DOIUrl":"https://doi.org/10.1007/s43630-025-00764-1","url":null,"abstract":"<p><p>The efficacy of photosensitizer (PS) for photodynamic therapy of malignant tumors depends significantly on its interaction with serum proteins. Albumin binds non-covalently with hydrophilic and amphiphilic PSs. However, a clear understanding on role of albumin in delivery of PS in cancer cells is still lacking. We explored the role of albumin in disaggregation and cellular uptake of Cycloimide Purpurin-18 (CIPp-18), an amphiphilic near-infrared PS, in human breast carcinoma (MCF-7) cells. Results show that CIPp-18 added to fetal bovine serum (FBS) associates mainly with albumin and its aggregation in neat buffer is completely reversed by addition of bovine serum albumin (BSA). Under serum-enriched condition, CIPp-18 accumulated efficiently in cells and localized mainly in cell membrane and ER but not in lysosomes. Accumulation of CIPp-18 in cells was not affected by inhibitors of metabolic energy but partially inhibited at cold temperature. Confocal microscopy studies on uptake of CIPp-18 complexed to FITC-labeled BSA revealed significant colocalization of CIPp-18 and FITC-BSA in the distinct regions of cell membrane at 15 min after incubation, whereas at 90 min, FITC albumin localized independently in endocytic vesicles. Studies on binding of CIPp-18 with albumin using intrinsic tryptophan fluorescence of BSA and in silico docking reveal that CIPp-18 binds near Sudlow site I but away from tryptophan residue. Significant quenching of fluorescence of BSA-bound CIPp-18 by iodide ions further confirms that CIPp-18 binds at the surface of albumin. These results together demonstrate that the cellular uptake of albumin-bound CIPp-18 is mediated via facilitated diffusion involving dissociation of CIPp-18 from albumin prior to endocytosis. Further, the binding of CIPp-18 on the surface of albumin appears to play a crucial role in disaggregation and cellular uptake of CIPp-18.</p>","PeriodicalId":520750,"journal":{"name":"Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of natural photosensitizers in dye-sensitized solar cells: opportunities, challenges, and future outlook.","authors":"Amrit Dutta, Bikashjyoti Gohain, Tran Thu Trang, James R Jennings, Nguyen Van Hao, Surajit Konwer, Ankur Gogoi, Piyasiri Ekanayake","doi":"10.1007/s43630-025-00762-3","DOIUrl":"https://doi.org/10.1007/s43630-025-00762-3","url":null,"abstract":"<p><p>As the global demand for renewable and sustainable energy solutions intensifies, dye-sensitized solar cells (DSSCs) have gained prominence as a promising alternative to conventional silicon-based photovoltaics. This review examines recent advancements in natural sensitizers for DSSCs, emphasizing their potential for enhancing light-harvesting efficiency while reducing environmental impact. The review begins by outlining the fundamental principles of DSSCs and the pivotal role of photosensitizers in these systems. A systematic analysis of recent literature has been conducted, focusing on the performance, stability, and practical applicability of various natural dye sources. The review highlights key achievements with natural dyes, including their potential to create sustainable, cost-effective, and eco-friendly DSSCs with reasonable efficiency. Additionally, persistent challenges, such as dye degradation and low long-term stability, are discussed, along with proposed strategies to address these limitations. This comprehensive assessment provides insights into future research directions and the practical feasibility of natural sensitizers in next-generation DSSC technology.</p>","PeriodicalId":520750,"journal":{"name":"Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144669292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circular dichroism spectroscopy reveals multiple phytochrome photoproducts in equilibrium.","authors":"Nathan C Rockwell, J Clark Lagarias","doi":"10.1007/s43630-025-00763-2","DOIUrl":"https://doi.org/10.1007/s43630-025-00763-2","url":null,"abstract":"<p><p>Phytochromes are a widespread family of red/far-red photoreceptors including master regulators of plant growth and development. Phytochromes use 15,16-photoisomerization of linear tetrapyrrole (bilin) chromophores to toggle between a 15Z red-absorbing dark-adapted state (P_r) and a 15E far-red-absorbing photoproduct (P_fr). The bilin is bound within a conserved, N-terminal PAS-GAF-PHY photosensor tridomain and is covalently attached to a conserved Cys residue, but the mechanism(s) permitting detection of far-red light are not well understood. Plant and cyanobacterial phytochromes exhibit complex P_fr CD spectra that are also not well explained. In this work, we use the model cyanobacterial phytochrome Cph1 from Synechocystis sp. PCC 6803 to examine the basis for this complex CD spectrum. We employ truncations with and without the PHY domain (N514 and N322) as well as a panel of variants with point substitutions in N514. We identify two classes of photoconversion: type 1 produces P_fr, whereas type 2 produces a blue-shifted alternative photoproduct (P_ALT) with a distinct CD spectrum and with properties similar to those of the previously observed Meta-R_C intermediate. Both type 1 and type 2 variants exhibit efficient photoisomerization, indicating that type 2 variants are specifically deficient in spectral tuning of the 15E photoproduct. Subtle differences within type 1 variants can be ascribed to the presence of varying amounts of P_ALT. We show that P_fr formation can proceed at pH 6 in Type 2 cases, whereas even wild-type N514 is unable to form P_fr at pH 9. We, thus, demonstrate that the photoproduct of Cph1 contains two 15E species in pH-dependent equilibrium, shedding new light on the P_fr state.</p>","PeriodicalId":520750,"journal":{"name":"Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144669293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced photocatalytic degradation of Ibuprofen using CuO-Polypyrrole/activated carbon hydrogel for sustainable water purification: characterization and application.","authors":"Mashael D Alqahtani, Amna M Farhan, May N Bin Jumah, Hassan A Rudayni, Ahmed A Allam, Mostafa R Abukhadra","doi":"10.1007/s43630-025-00758-z","DOIUrl":"https://doi.org/10.1007/s43630-025-00758-z","url":null,"abstract":"<p><p>The contamination of aquatic environments by pharmaceutical residues, particularly ibuprofen (IBU), necessitates effective removal strategies. This study presents the synthesis and characterization of CuO-polypyrrole/activated carbon hydrogel (Cu@Ppy/AC) as a photocatalyst for IBU degradation under visible light. Characterization techniques including XRD, FTIR, SEM, and BET confirmed the structural and physicochemical properties of Cu@Ppy/AC. Photocatalytic performance was optimized by investigating pH, catalyst dosage, IBU concentration, and reaction time. Under optimal conditions (0.5 g/L catalyst, pH 6, 5 mg/L IBU), complete degradation occurred within 40 min, followed by total mineralization within 80 min. The reaction followed first-order kinetics (k = 0.0365 min⁻¹), with hydroxyl radicals (OH•) and superoxide radicals (O₂•⁻) identified as key oxidative species. The oxidation pathway proceeded through a sequence of reactions including hydroxylation, decarboxylation, and cleavage of the aromatic ring structure, ultimately resulting in the full mineralization of ibuprofen into carbon dioxide (CO₂) and water (H₂O). The synergistic interaction between CuO, polyaniline hydrogel, and activated carbon enhanced charge separation efficiency, improving catalytic activity under visible light irradiation. Toxicological assessments using HEK293T and HepG2 cell lines revealed that partially oxidized IBU intermediates exhibited transient cytotoxicity, which diminished upon complete mineralization. Cu@Ppy/AC retained over 85% efficiency after 7 cycles, demonstrating high stability and recyclability. Comparative analysis highlighted its superior degradation efficiency and material sustainability over other reported photocatalysts. These findings establish Cu@Ppy/AC as a cost-effective and sustainable photocatalyst for pharmaceutical wastewater treatment, with potential for large-scale application and remediation of multiple pharmaceutical contaminants.</p>","PeriodicalId":520750,"journal":{"name":"Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photosensitizer-conjugated lignin nanoparticles for photodynamic antimicrobial inactivation.","authors":"Chenyu Jiang, Parisa Sadrpour, Lihan Chen, Frank Scholle, Anka Veleva, Orlin D Velev, Reza A Ghiladi","doi":"10.1007/s43630-025-00761-4","DOIUrl":"https://doi.org/10.1007/s43630-025-00761-4","url":null,"abstract":"<p><p>Towards the goal of developing broad-spectrum, self-disinfecting, and cost-effective renewable antimicrobial materials for the prevention of pathogen transmission relevant to healthcare-associated infections (HAIs), a facile cyanuric chloride coupling strategy was employed to covalently attach a commercially available phenothiazine-based photosensitizer, thionine (Th), onto lignin nanoparticles (LNPs). The resultant photodynamic Th-LNPs conjugate was characterized by a variety of techniques, including elemental analysis, diffuse reflectance UV-visible and energy-dispersive X-ray spectroscopies, scanning electron microscopy, and thermal gravimetric analysis. Antibacterial photodynamic inactivation studies demonstrated high levels of inactivation (99.999+%) by the Th-LNPs against Gram-positive pathogens methicillin-resistant Staphylococcus aureus (MRSA; ATCC-44) and vancomycin-resistant Enterococcus faecium (VRE; ATCC-2320), and Gram-negative multi-drug-resistant Acinetobacter baumannii (MDRAB; ATCC-1605), upon visible light illumination (60 min, 400-700 nm, 65 ± 5 mW/cm²). Vesicular stomatitis virus (VSV), employed as a model enveloped virus, exhibited similarly high levels of inactivation (99.996%) under the same illumination conditions. Taken together, these results suggest that applications that are agnostic to the chemical and structural heterogeneity of the polymer, such as photodynamic inactivation, can enable the valorization of lignin from a low-value industrial byproduct into a potent, scalable and renewable antimicrobial material via a facile two-step synthesis.</p>","PeriodicalId":520750,"journal":{"name":"Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144639583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"457 nm LED blue light inhibits oral squamous cell carcinoma cell proliferation via the PI3K/AKT pathway and autophagy.","authors":"Hui Jiang, Jiali Yang, Qiqi Fu, Haokuan Qin, Muqing Liu","doi":"10.1007/s43630-025-00746-3","DOIUrl":"10.1007/s43630-025-00746-3","url":null,"abstract":"<p><strong>Background: </strong>Photobiomodulation (PBM) therapy, utilizing light to regulate cellular processes, holds promise as a cancer treatment. We aimed to explore PBM's impact on two oral squamous cell carcinoma (OSCC) cell lines, Cal-27 and SCC-25, under varying blue light irradiation conditions.</p><p><strong>Methods: </strong>Cal-27 and SCC-25 cells were exposed to 457 nm blue light at varying irradiances (2.5-70 mW/cm²) and radiant exposure levels (3-84 J/cm²). Cell viability, reactive oxygen species levels, mitochondrial function, apoptosis, proliferation, and proteins related to proliferation and autophagy were quantified.</p><p><strong>Results: </strong>457 nm blue light significantly reduced OSCC cell viability, with efficacy increasing proportionally to radiant exposure. This effect resulted primarily from inhibited cell proliferation, as we observed no significant apoptosis or necrosis. Additionally, blue light elevated reactive oxygen species levels in OSCC cells while selectively decreasing mitochondrial membrane potential in SCC-25 cells, suggesting variable cell-type responses. Besides, autophagy levels were modulated by blue light exposure.</p><p><strong>Conclusion: </strong>The study demonstrates that 457 nm blue LED photobiomodulation therapy inhibits OSCC cell progression by promoting autophagy and inhibiting PI3K/AKT pathway-mediated cell proliferation. These insights provide a basis for exploring therapeutic applications of photobiomodulation in oral cancer treatment.</p>","PeriodicalId":520750,"journal":{"name":"Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology","volume":" ","pages":"1079-1092"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144328471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TiO₂@ZIF-8 hybrid as a type II heterojunction photocatalyst:adsorption/photocatalytic properties, kinetics, and effect of humidity.","authors":"A Neren Ökte, Duygu Tuncel","doi":"10.1007/s43630-025-00754-3","DOIUrl":"10.1007/s43630-025-00754-3","url":null,"abstract":"<p><p>TiO₂@ZIF-8 hybrid and its humidified forms are synthesized as type 2 heterojunction systems using a facile sol-gel method at different relative humidity (RH) conditions. The hybrids' surface characteristics and optical properties are thoroughly examined, followed by investigations into adsorption capacities and photocatalytic activities using anionic-methyl orange (MO) and cationic-methylene blue (MB) dyes. TiO₂@ZIF-8 hybrid generates a mesoporous structure with a higher surface area (135.6 m² g^-1) than bare TiO₂ (40.1 m² g^-1). Band gap energies of TiO₂@ZIF-8 (3.00 eV) and humidified hybrids (2.95-2.91 eV) decrease compared to the individual phases of TiO₂ (3.08 eV) and ZIF-8 (4.9 eV). XPS analysis verifies the electron donation from ZIF-8 to TiO₂. Adsorptions of MO and MB differ depending on the affinities of MO or MB's functional groups on the TiO₂@ZIF-8 and humidified hybrids. TiO₂@ZIF-8 demonstrates 98.7% MO and 89.5% MB degradations within 100 min irradiation. Dark adsorption studies follow Langmuir model with Langmuir constants of K_L = 0.445 L mg^-1 for MO in the presence of TiO₂@ZIF-8 and K_L = 0.409 L mg^-1 for MB in the presence of TiO₂@ZIF-8 (84% RH). Meanwhile, pseudo-second-order kinetic model is applicable for dark experiments with rate constants of k₂ = 0.918 g mg^-1 min^-1 for MO in the existence of TiO₂@ZIF-8 and k₂ = 0.917 g mg^-1 min^-1 for MB in the existence of TiO₂@ZIF-8 (84% RH). Under irradiation, TiO₂@ZIF-8 (84% RH) has higher first-order rate constant 'k' values for both MO (0.0134 min^-1) and MB (0.0146 min^-1) relative to that of TiO₂@ZIF-8 (0.0129 min^-1 for MO and 0.0112 min^-1 for MB). Based on Langmuir-Hinshelwood model, TiO₂@ZIF-8 (84% RH)) shows the highest adsorption coefficients (K) of 0.164 L mg^-1 for MO and 0.192 L mg^-1 for MB and rate constants (k) of 0.197 mg L^-1 min^-1 for MO and 0.182 mg L^-1 min^-1 for MB. Notably, the hybrids maintain high stability and photocatalytic efficiency after four consecutive degradation cycles, highlighting their potential for sustainable environmental remediation.</p>","PeriodicalId":520750,"journal":{"name":"Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology","volume":" ","pages":"1107-1126"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A NIR dual-channel fluorescent probe for the detection of cyanide in living cells.","authors":"Yong-Qiang Xie, Qiang Suo, Shan Jiang, Lin Li, Chun-Yan Ren, Rong Wu, Xiao-Ning Shi, Xiao-Qiang Zhao, Li-Na Wang, Reng-Jie Yin, Hui Chen, Hao-Bo Zhang","doi":"10.1007/s43630-025-00748-1","DOIUrl":"10.1007/s43630-025-00748-1","url":null,"abstract":"<p><p>This study details the design and synthesis of a near-infrared (NIR) dual-channel cyanide detection fluorescent probe based on triphenylamine-hemicyanine. TPA-CY successfully utilizes the NIR fluorescence properties of triphenylamine-hemicyanine conjugates, showcasing good selectivity and sensitivity, facilitating the visual monitoring of cyanide anions in living cells. Interaction between TPA-CY and CN^- occurs rapidly, within a mere 25 s. The sensitivity of TPA-CY to cyanide ions is good, with detection thresholds reaching 0.60 µM and 0.66 µM for UV and fluorescence spectra, respectively. The detection mechanism of this probe is the nucleophilic addition of CN^- to the C = N bond, which hindered intramolecular charge transfer. Moreover, the probe's efficacy in tracking cyanide radicals in biological systems is validated through live cell imaging.</p>","PeriodicalId":520750,"journal":{"name":"Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology","volume":" ","pages":"1093-1105"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144328472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}