Open medicine (Warsaw, Poland)最新文献_第8页

Possible impact of COVID-19 pandemic and lockdown on suicide behavior among patients in Southeast Serbia. COVID-19大流行和封锁对塞尔维亚东南部患者自杀行为的可能影响。

IF 2.1

Open medicine (Warsaw, Poland) Pub Date : 2022-06-07 eCollection Date: 2022-01-01 DOI: 10.1515/med-2022-0488

Suzana Tosic Golubovic, Olivera Zikic, Gordana Nikolic, Jelena Kostic, Maja Simonovic, Iva Binic, Uros Gugleta

{"title":"Possible impact of COVID-19 pandemic and lockdown on suicide behavior among patients in Southeast Serbia.","authors":"Suzana Tosic Golubovic, Olivera Zikic, Gordana Nikolic, Jelena Kostic, Maja Simonovic, Iva Binic, Uros Gugleta","doi":"10.1515/med-2022-0488","DOIUrl":"https://doi.org/10.1515/med-2022-0488","url":null,"abstract":"Individuals with serious mental illness are more affected by emotional reactions, including suicidal behavior due to COVID-19 and psychosocial consequences of pandemic. The current cross-sectional study aimed to explore the possible association of COVID-19 and suicidal behavior (suicide ideation and attempt) before and during pandemic-associated lockdown in Serbia. We retrospectively reviewed the clinical records of 104 adult psychiatric inpatients admitted at Psychiatric Clinic, University Clinic Center Niš, Serbia, after ending lockdown and compared the obtained results with 181 adult psychiatric inpatients admitted during the same period in 2019 and 2018. Suicide ideation were more frequent in 2020 comparing with 2019 and 2018 (25 vs 12.5%, vs 9.41%; p < 0.05). Around 28% of patients with suicide attempts were exposed daily to the information related to COVID-19 coming from social media, while this frequency was significantly lower, only 7.55% (p < 0.1), among patients with no suicide ideation or attempts. Adjustment disorder was more frequent among patients with suicide attempts in comparison to the patients with suicide ideation (32 vs 11%), especially in patients without suicide ideation and attempts (32 vs 0%, p < 0.001). Of all studied patients with suicide attempts during 2020, 60% were not in the previous psychiatric treatment before admission.","PeriodicalId":520729,"journal":{"name":"Open medicine (Warsaw, Poland)","volume":" ","pages":"1045-1056"},"PeriodicalIF":2.1,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175012/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40477049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Smog and risk of maternal and fetal birth outcomes: A retrospective study in Baoding, China. 雾霾与孕产妇和胎儿分娩结局的风险:中国保定的回顾性研究。

IF 2.1

Open medicine (Warsaw, Poland) Pub Date : 2022-05-31 eCollection Date: 2022-01-01 DOI: 10.1515/med-2022-0489

Yijing Zhai, Bei Wang, Liqiang Qin, Bin Luo, Ying Xie, Huanyu Hu, Hongzhen Du, Zengning Li

{"title":"Smog and risk of maternal and fetal birth outcomes: A retrospective study in Baoding, China.","authors":"Yijing Zhai, Bei Wang, Liqiang Qin, Bin Luo, Ying Xie, Huanyu Hu, Hongzhen Du, Zengning Li","doi":"10.1515/med-2022-0489","DOIUrl":"https://doi.org/10.1515/med-2022-0489","url":null,"abstract":"Pregnant women are more susceptible to smog pollution than the general population. This study focused on the association between smog and birth outcomes, considering both pregnant mothers and their offspring. In this retrospective study, conducted in Baoding between 2013 and 2016, we enrolled 842 participants. Birth outcomes were low birth weight (LBW), pregnancy-induced hypertension (PIH), gestational diabetes mellitus (GDM), and premature rupture of membranes (PROM). The overall prevalence of LBW, PIH, GDM, and PROM was 8.2%, 14.8%, 16.5%, and 12.1%, respectively. Compared with lower pollution level, higher pollution level of fine particulate matter (particulate matter with aerodynamics diameter <2.5 μm) (PM2.5), inhalable particle (particulate matter with aerodynamics diameter <10 μm) (PM10), and CO increased the risk of term with LBW. PM2.5, PM10, and NO2 increased the risk of PIH during different trimesters, while PM10 increased the risk of PROM during trimester 3. In conclusion, smog significantly affects the risk of adverse birth outcomes by different exposure time windows.","PeriodicalId":520729,"journal":{"name":"Open medicine (Warsaw, Poland)","volume":" ","pages":"1007-1018"},"PeriodicalIF":2.1,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9164291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40224438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive analysis of molecular mechanism and a novel prognostic signature based on small nuclear RNA biomarkers in gastric cancer patients. 基于小核RNA生物标志物的胃癌患者预后特征及分子机制综合分析

IF 2.1

Open medicine (Warsaw, Poland) Pub Date : 2022-05-30 eCollection Date: 2022-01-01 DOI: 10.1515/med-2022-0493

Ziyu Liang, Dongxing Su, Kang Liu, Haixing Jiang

{"title":"Comprehensive analysis of molecular mechanism and a novel prognostic signature based on small nuclear RNA biomarkers in gastric cancer patients.","authors":"Ziyu Liang, Dongxing Su, Kang Liu, Haixing Jiang","doi":"10.1515/med-2022-0493","DOIUrl":"https://doi.org/10.1515/med-2022-0493","url":null,"abstract":"Small nuclear RNAs (snRNAs) are rarely reported in cancer. This study is based on The Cancer Genome Atlas genome-wide data set to explore the prognostic value and molecular mechanism of snRNAs in gastric cancer (GC). Gene ontology, Kyoto Encyclopedia of Genes and Genomes, and gene set enrichment analysis were used to explore the molecular mechanism of snRNAs. A total of 351 patients were included in the survival analysis, and 14 prognostic snRNAs were identified using multivariate survival analysis. We constructed a prognostic signature containing nine snRNAs, which can signally classify patients into high- and low-risk phenotypes (adjusted P < 0.0001, hazard ratio = 2.671, 95% confidence interval = 1.850-3.858). Combining the molecular mechanisms obtained by the three functional enrichment approaches, we concluded that this prognostic signature snRNAs participated in classical tumor-related signaling pathways, including Notch, PI3K, toll-like receptor, etc.; cell adhesion; cell cycle; cell proliferation; and other biological processes that affect the biological phenotype of cancer cells. We also found significant downregulation of the abundance of immune cell infiltrates and immune microenvironment scores for high-risk phenotypes of GC patients. In conclusion, this study has identified 14 prognostic snRNAs signally associated with GC overall survival and also constructed a novel prognostic signature containing nine prognostic snRNAs.","PeriodicalId":520729,"journal":{"name":"Open medicine (Warsaw, Poland)","volume":" ","pages":"991-1006"},"PeriodicalIF":2.1,"publicationDate":"2022-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9164292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40224437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 16-gene signature associated with homologous recombination deficiency for prognosis prediction in patients with triple-negative breast cancer. 同源重组缺陷与三阴性乳腺癌患者预后预测相关的16个基因特征

IF 2.1

Open medicine (Warsaw, Poland) Pub Date : 2022-05-11 eCollection Date: 2022-01-01 DOI: 10.1515/med-2022-0475

Daodu Wang, Yifeng Shi, Hanyang Huang, Qijiong Zhao, Yongyue He, Wenzhi Su

{"title":"A 16-gene signature associated with homologous recombination deficiency for prognosis prediction in patients with triple-negative breast cancer.","authors":"Daodu Wang, Yifeng Shi, Hanyang Huang, Qijiong Zhao, Yongyue He, Wenzhi Su","doi":"10.1515/med-2022-0475","DOIUrl":"https://doi.org/10.1515/med-2022-0475","url":null,"abstract":"Homologous recombination deficiency (HRD) commonly occurs in breast cancer, which is the second cause of cancer death in women with a high rate of relapse and poor outcomes. Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer. Thus, we aim to develop a prognostic signature based on HRD expecting to help improve outcomes in TNBC. The Cancer Genome Atlas (TCGA)-TNBC cohort was divided into the training set and the testing set randomly. Sixteen genes were filtered from the prognostic HRD-associated genes to establish a prognostic model in the training set. Patients were divided into high-risk and low-risk groups based on the median value of the risk score. Prognosis analysis showed that the high-risk group was associated with a worse prognosis in the training set, the testing set, the entire TCGA-TNBC cohort, and the METABRIC-TNBC cohort. The time-dependent receiver operating characteristic curve showed that our model had very good accuracy in the prediction of 1-5-year overall survival in the TCGA-TNBC cohort. Besides, a comparison of the area under curve value and C-index between our model and four published models showed that our model had the best predictive efficiency compared to other models. Subsequently, a nomogram was established. Finally, our finding also indicated that our model was associated with immunoregulation in TNBC and had the potential to be the target for TNBC treatment. Therefore, our findings not only provided a new strategy in the personalized prognosis management of TNBC but also offered new insight into precision treatment in TNBC.","PeriodicalId":520729,"journal":{"name":"Open medicine (Warsaw, Poland)","volume":" ","pages":"882-896"},"PeriodicalIF":2.1,"publicationDate":"2022-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40599170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artemisinin protects against cerebral ischemia and reperfusion injury via inhibiting the NF-κB pathway. 青蒿素通过抑制NF-κB通路对脑缺血再灌注损伤具有保护作用。

IF 2.1

Open medicine (Warsaw, Poland) Pub Date : 2022-05-11 eCollection Date: 2022-01-01 DOI: 10.1515/med-2022-0435

Hui Ji, Haifeng Jin, Guangwei Li, Li Jin, Xiaoxu Ren, Ying Lv, Yuchun Wang

{"title":"Artemisinin protects against cerebral ischemia and reperfusion injury via inhibiting the NF-κB pathway.","authors":"Hui Ji, Haifeng Jin, Guangwei Li, Li Jin, Xiaoxu Ren, Ying Lv, Yuchun Wang","doi":"10.1515/med-2022-0435","DOIUrl":"https://doi.org/10.1515/med-2022-0435","url":null,"abstract":"This study investigated whether artemisinin (ART) exerts a neuroprotective effect against cerebral ischemia/reperfusion (I/R) injury. Hypoxia-glucose deprivation and reoxygenation (OGD/R) of SH-SY5Y cells were used as the I/R injury model in vitro. Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and lactate dehydrogenase (LDH) release was measured. Cell apoptosis and apoptosis-associated protein expression were determined via flow cytometry and western blotting, respectively. The levels of glutathione peroxidase, superoxide dismutase, catalase, and malondialdehyde were determined. The secretion of tumor necrosis factor-α and interleukin-1β was measured using ELISA. The activation of the nuclear factor kappa B (NF-κB) pathway was also determined. The indicated ART concentrations (0, 25, 50, 75, and 100 μM) had no significant effect on SH-SY5Y cell viability and LDH activity. ART promoted cell viability, reduced cell apoptosis, repressed cellular inflammation, and inhibited cellular oxidative stress and NF-κB signaling pathway in OGD/R-induced SH-SY5Y cells. In addition, all the protective effects of ART on OGD/R-induced SH-SY5Y cell injury were significantly reversed by an NF-κB agonist. In conclusion, ART protects neurons from OGD/R-induced damage in vitro by inhibiting the NF-κB signaling pathway. These results suggest that ART may be a potential agent for the treatment of cerebral I/R injury.","PeriodicalId":520729,"journal":{"name":"Open medicine (Warsaw, Poland)","volume":" ","pages":"871-881"},"PeriodicalIF":2.1,"publicationDate":"2022-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096231/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40599173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Sonoclot's usefulness in prediction of cardiopulmonary arrest prognosis: A proof of concept study. 超声心动图在预测心肺骤停预后中的有效性:一项概念验证研究。

IF 2.1

Open medicine (Warsaw, Poland) Pub Date : 2022-03-02 eCollection Date: 2022-01-01 DOI: 10.1515/med-2022-0447

Yumi Ichikawa, Kei Kawano, Mizuki Mori, Ayumi Numazaki, Yuto Aramaki, Kazunori Fukushima, Yuta Isshiki, Yusuke Sawada, Jun Nakajima, Kiyohiro Oshima

{"title":"Sonoclot's usefulness in prediction of cardiopulmonary arrest prognosis: A proof of concept study.","authors":"Yumi Ichikawa, Kei Kawano, Mizuki Mori, Ayumi Numazaki, Yuto Aramaki, Kazunori Fukushima, Yuta Isshiki, Yusuke Sawada, Jun Nakajima, Kiyohiro Oshima","doi":"10.1515/med-2022-0447","DOIUrl":"https://doi.org/10.1515/med-2022-0447","url":null,"abstract":"The aim of the present study was to evaluate the usefulness of measuring whole blood coagulation with Sonoclot to predict return of spontaneous circulation (ROSC) in patients with out-of-hospital cardiopulmonary arrest (OHCA). This was a prospective, observational clinical study on patients with OHCA who were transferred to our emergency department between August 2016 and July 2018. Patients were divided into two groups: patients with return of spontaneous circulation (ROSC[+] group) and those without (ROSC[-] group). We compared the activated clotting time (ACT), clot rate (CR), and platelet function (PF) as measured with Sonoclot, and the fibrinogen degradation products (FDP) level and D-dimer level between the two groups. We analyzed 87 patients: 37 in the ROSC(+) and 50 in the ROSC(-) groups. Regarding ACT, CR, PF, FDP, and D-dimer, we used receiver operating characteristic (ROC) curves to examine how well each factor predicts ROSC. The area under the ROC curve (AUC) of CR was higher than that of the FDP and D-dimer levels. Among patients with cardiogenic cardiac arrest, the AUC of CR was higher than the AUCs of other coagulation factors. In conclusion, viscoelastic blood coagulation measurements using Sonoclot may be useful for predicting ROSC in OHCA patients.","PeriodicalId":520729,"journal":{"name":"Open medicine (Warsaw, Poland)","volume":" ","pages":"414-422"},"PeriodicalIF":2.1,"publicationDate":"2022-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8893265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40324287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-29c-5p knockdown reduces inflammation and blood-brain barrier disruption by upregulating LRP6. miR-29c-5p敲低通过上调LRP6减少炎症和血脑屏障破坏。

IF 2.1

Open medicine (Warsaw, Poland) Pub Date : 2022-02-22 eCollection Date: 2022-01-01 DOI: 10.1515/med-2022-0438

Qijun Dai, Jian Sun, Tianyi Dai, Qin Xu, Yueqin Ding

{"title":"miR-29c-5p knockdown reduces inflammation and blood-brain barrier disruption by upregulating LRP6.","authors":"Qijun Dai, Jian Sun, Tianyi Dai, Qin Xu, Yueqin Ding","doi":"10.1515/med-2022-0438","DOIUrl":"https://doi.org/10.1515/med-2022-0438","url":null,"abstract":"Blood-brain barrier participates in the pathological process of ischemic stroke. MicroRNA-29c-5p was highly expressed in clinical samples from patients with ischemic stroke. In this study, oxygen-glucose deprivation (OGD) treatment of astrocytes enhanced the permeability of brain microvascular endothelial cells (BMECs), and the miR-29c-5p expression was elevated in clinical samples from patients with ischemic stroke. For the function of miR-29c-5p in ischemic stroke, the miR-29c-5p knockdown decreased the permeability and the tight junction protein (TJP) destruction of BMECs and ameliorated the inflammation induced by OGD-treated astrocytes. Mechanistically, miR-29c-5p interacted with lipoprotein receptor-related protein 6 (LRP6) and negatively regulated the LRP6 expression in astrocytes. Moreover, the rescue assays indicated that the interference with miR-29c-5p ameliorated the TJP destruction of BMECs and inflammation caused by OGD-treated astrocytes by increasing the LRP6 expression. Together, miR-29c-5p knockdown decreased the high permeability and the TJP destruction of BMECs and ameliorated the inflammation induced by OGD-treated astrocytes by elevating LRP6 expression.","PeriodicalId":520729,"journal":{"name":"Open medicine (Warsaw, Poland)","volume":" ","pages":"353-364"},"PeriodicalIF":2.1,"publicationDate":"2022-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40571033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cardiac contractility modulation ameliorates myocardial metabolic remodeling in a rabbit model of chronic heart failure through activation of AMPK and PPAR-α pathway. 心脏收缩调节通过激活AMPK和PPAR-α途径改善兔慢性心力衰竭模型的心肌代谢重塑。

IF 2.1

Open medicine (Warsaw, Poland) Pub Date : 2022-02-22 eCollection Date: 2022-01-01 DOI: 10.1515/med-2022-0415

Feifei Zhang, Litian Liu, Yuetao Xie, Jiaqi Wang, Xuefeng Chen, Shihang Zheng, Yingxiao Li, Yi Dang

{"title":"Cardiac contractility modulation ameliorates myocardial metabolic remodeling in a rabbit model of chronic heart failure through activation of AMPK and PPAR-α pathway.","authors":"Feifei Zhang, Litian Liu, Yuetao Xie, Jiaqi Wang, Xuefeng Chen, Shihang Zheng, Yingxiao Li, Yi Dang","doi":"10.1515/med-2022-0415","DOIUrl":"https://doi.org/10.1515/med-2022-0415","url":null,"abstract":"Abstract Metabolic remodeling contributes to the pathological process of heart failure (HF). We explored the effects of cardiac contractility modulation (CCM) on myocardial metabolic remodeling in the rabbit model with HF. The HF in rabbit model was established by pressure uploading and then CCM was applied. We evaluated the cardiac structure and function by echocardiography, serum BNP level, and hematoxylin and eosin and Masson’s trichrome staining. We detected the accumulation of glycogen and lipid droplets in myocardial tissues by periodic acid-Schiff and Oil Red O staining. Then, we measured the contents of glucose, free fatty acid (FFA), lactic acid, pyruvate, and adenosine triphosphate (ATP) levels in myocardial tissues by corresponding kits and the expression levels of key factors related to myocardial substrate uptake and utilization by western blotting were analyzed. CCM significantly restored the cardiac structure and function in the rabbit model with HF. CCM therapy further decreased the accumulation of glycogen and lipid droplets. Furthermore, CCM reduced the contents of FFA, glucose, and lactic acid, and increased pyruvate and ATP levels in HF tissues. The protein expression levels related to myocardial substrate uptake and utilization were markedly improved with CCM treatment by further activating adenosine monophosphate-activated protein kinase and peroxisome proliferator-activated receptor-α signaling pathways.","PeriodicalId":520729,"journal":{"name":"Open medicine (Warsaw, Poland)","volume":" ","pages":"365-374"},"PeriodicalIF":2.1,"publicationDate":"2022-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40569095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Quercitrin protects human bronchial epithelial cells from oxidative damage. 槲皮素保护人支气管上皮细胞免受氧化损伤。

IF 2.1

Open medicine (Warsaw, Poland) Pub Date : 2022-02-22 eCollection Date: 2022-01-01 DOI: 10.1515/med-2022-0416

Dan Yu, Fan Wang, Shuming Ye, Shuo Yang, Ning Yu, Xinyan Zhou, Nian Zhang

{"title":"Quercitrin protects human bronchial epithelial cells from oxidative damage.","authors":"Dan Yu, Fan Wang, Shuming Ye, Shuo Yang, Ning Yu, Xinyan Zhou, Nian Zhang","doi":"10.1515/med-2022-0416","DOIUrl":"https://doi.org/10.1515/med-2022-0416","url":null,"abstract":"Chronic obstructive pulmonary disease (COPD) is mainly caused by cigarette smoking (CS), with oxidative stress being one key component during its pathogenesis. This study aimed to investigate the effects of quercitrin (QE) on cigarette smoke extract (CSE)-induced cell apoptosis and oxidative stress in human bronchial epithelial cells (HBECs) and its underlying mechanism. HBECs were treated with 2% CSE for 24 h to establish in vitro COPD cellular models. CCK-8 assay and flow cytometry analysis were performed to evaluate cell viability and apoptosis, respectively. Western blotting was applied to examine protein levels and ELISA kits were used to examine contents of the indicated oxidant/antioxidant markers. The results demonstrated that CSE promoted apoptosis and suppressed viability of HBECs and QE reversed these effects. CSE caused increase in T-AOC, superoxide dismutase, and glutathione (GSH) peroxidase contents and decrease in MDA, reactive oxygen species , and GSH contents in HBECs, which were rescued by QE treatment. The CSE-induced Nrf2 nuclear translocation and elevation of NAD(P)H: quinone oxidoreductase 1 (NQO1) and heme oxygenase-1 (HO-1) expression were also reversed by QE in HBECs. The mitogen-activated protein kinase (MAPK) signaling was activated by CSE and further suppressed by QE in HBECs. Collectively, QE exerts a protective role in HBECs against cell apoptosis and oxidative damage via inactivation of the Nrf2/HO-1/NQO1 pathway and the MAPK/ERK pathway.","PeriodicalId":520729,"journal":{"name":"Open medicine (Warsaw, Poland)","volume":" ","pages":"375-383"},"PeriodicalIF":2.1,"publicationDate":"2022-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40571034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Atypical symptoms of malignant hyperthermia: A rare causative mutation in the RYR1 gene. 恶性高热的非典型症状:RYR1基因中一种罕见的致病突变。

IF 2.1

Open medicine (Warsaw, Poland) Pub Date : 2022-02-02 eCollection Date: 2022-01-01 DOI: 10.1515/med-2021-0396

Qiao Ling Wang, Yu Fang, Shuo Guo Jin, Jing Tao Liang, Yi Feng Ren

{"title":"Atypical symptoms of malignant hyperthermia: A rare causative mutation in the RYR1 gene.","authors":"Qiao Ling Wang, Yu Fang, Shuo Guo Jin, Jing Tao Liang, Yi Feng Ren","doi":"10.1515/med-2021-0396","DOIUrl":"https://doi.org/10.1515/med-2021-0396","url":null,"abstract":"Malignant hyperthermia (MH) is an autosomal dominant genetic condition of the skeletal muscle triggered by inhaled general anesthetic agents or succinylcholine and associated with a hypermetabolic state and skeletal muscle rigidity. Tachycardia, increased carbon dioxide production, hypercarbia, hyperthermia, acidosis, hyperkalemia, cardiac arrhythmias, muscle rigidity, and rhabdomyolysis are common symptoms of MH. As the progression of the syndrome could be rapid or less evident, even experienced physicians have difficulty in diagnosing MH, which can lead to delays in treatment and increased mortality. We report a rare case of a 36-year-old man, who underwent open reduction and internal fixation of the left clavicle after inhaled anesthetics. The patient developed dyspnea, hypotension, unremitting hyperthermia, tachycardia, and elevated serum myoglobin, and finally died of pyemia and disseminated intravascular coagulation. We reviewed the process of disease development, summarized the steps of diagnosis, and improved genetic testing. Exome sequencing revealed a new mutation c.8519G>A (p.arg2840 GLN) in the RYR1 gene that could be associated with MH. The gene mutation was also found in his daughter's genetic test. This case emphasized the importance of the awareness of MH and its atypical clinical symptoms. The presence of dyspnea, hypotension, unremitting hyperthermia, tachycardia, and raised myoglobin in serum might further strengthen the clinical diagnosis of suspected MH.","PeriodicalId":520729,"journal":{"name":"Open medicine (Warsaw, Poland)","volume":" ","pages":"239-244"},"PeriodicalIF":2.1,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8812711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39809881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2