Journal of chromatography. B, Analytical technologies in the biomedical and life sciences最新文献

{"title":"Biological monitoring and analytical toxicology in occupational and environmental medicine.","authors":"Michael Bader, Thomas Göen","doi":"10.1016/j.jchromb.2010.08.025","DOIUrl":"https://doi.org/10.1016/j.jchromb.2010.08.025","url":null,"abstract":"","PeriodicalId":520661,"journal":{"name":"Journal of chromatography. B, Analytical technologies in the biomedical and life sciences","volume":" ","pages":"2465-6"},"PeriodicalIF":3.0,"publicationDate":"2010-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jchromb.2010.08.025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40061683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunogold-silver staining-on-a-chip biosensor based on cross-flow chromatography.","authors":"Il-Hoon Cho,&nbsp;Sung-Min Seo,&nbsp;Eui-Hwan Paek,&nbsp;Se-Hwan Paek","doi":"10.1016/j.jchromb.2009.07.016","DOIUrl":"https://doi.org/10.1016/j.jchromb.2009.07.016","url":null,"abstract":"<p><p>Immunogold-silver staining (IGSS) was adopted in cross-flow chromatographic analysis in which immunological reactions and silver intensification were sequentially conducted in the vertical and horizontal directions, respectively. Factors controlling the performance, except the silver substrate solution, were optimized to increase the signal-to-background ratio in measurements of cardiac troponin I as a model analyte. In generating the signal, the size of colloidal gold catalyst was critical; the smallest size (5-nm diameter) in the selected range yielded the highest colorimetric signal. To maintain the low background, two processes, blocking the remaining surfaces of membrane after antibody immobilization and washing the residual tracer after immunological reaction, were necessary. Self-nucleation of silver ions also caused a background signal and was controlled to some degree by decreasing the hydrodynamic force that arose when the substrate solution was supplied in the horizontal direction. Finally, a new chip (IGSS-on-a-chip; IOC) that allowed for convenient, efficient IGSS was produced by injection molding of plastic. This method enhanced the detection capability by 51-fold compared to the conventional rapid test kit using 30nm-sized colloidal gold as the tracer. The IOC biosensor results also showed that silver intensification yield via cross flow after immunological reaction was 19% higher than that by traditional incubation.</p>","PeriodicalId":520661,"journal":{"name":"Journal of chromatography. B, Analytical technologies in the biomedical and life sciences","volume":" ","pages":"271-7"},"PeriodicalIF":3.0,"publicationDate":"2010-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jchromb.2009.07.016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40008592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Total on-line analysis of a target protein from plasma by immunoextraction, digestion and liquid chromatography-mass spectrometry.","authors":"A Cingöz,&nbsp;F Hugon-Chapuis,&nbsp;V Pichon","doi":"10.1016/j.jchromb.2009.07.032","DOIUrl":"https://doi.org/10.1016/j.jchromb.2009.07.032","url":null,"abstract":"<p><p>A total on-line analysis of a target protein from a plasma sample was made using a selective immunoextraction step coupled on-line to an immobilized enzymatic reactor (IMER) for the protein digestion followed by LC-MS/MS analysis. For the development of this device, cytochrome c was chosen as model protein due to its well-known sequence. An immunosorbent (IS) based on the covalent immobilization of anti-cytochrome c antibodies on a solid support was made and an immunoextraction procedure was carefully developed to assess a selective extraction of the target protein from plasma. For the first time, IS was easily coupled on-line with a laboratory-made IMER based on pepsin. The whole on-line device (IS-IMER-LC-MS/MS) allowed the quantification of cytochrome c from 8.5pmol to 1.7nmol in buffer medium. Finally, this device was applied to the analysis of only 85pmol of cytochrome c from plasma with a RSD value lower than 10% (n=3).</p>","PeriodicalId":520661,"journal":{"name":"Journal of chromatography. B, Analytical technologies in the biomedical and life sciences","volume":" ","pages":"213-21"},"PeriodicalIF":3.0,"publicationDate":"2010-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jchromb.2009.07.032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40021564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stable-isotope dilution LC-ESI-MS/MS techniques for the quantification of total homocysteine in human plasma.","authors":"Michela Tomaiuolo,&nbsp;Gennaro Vecchione,&nbsp;Maurizio Margaglione,&nbsp;Daniela Pisanelli,&nbsp;Elvira Grandone","doi":"10.1016/j.jchromb.2009.07.024","DOIUrl":"https://doi.org/10.1016/j.jchromb.2009.07.024","url":null,"abstract":"<p><p>Homocysteine is an endogenous sulphydryl aminoacid irreversibly catabolized by transsulfuration to cysteine or remethylated to methionine. Increased plasma levels of homocysteine are an independent risk factor for atherosclerosis and cardiovascular disease. Accurate and reliable quantification of this amino acid in plasma samples is essential in clinical practice to explore the presence of a hyperhomocysteinemia, for instance after an ischemic event, or to control a possible adjunctive risk factor in patients at higher risk. In this review, LC-ESI-MS/MS methods are discussed and compared with other analytical methods for plasma homocysteine. LC-ESI-MS/MS is a technique combining the physicochemical separation of liquid chromatography with the analysis of mass spectrometry. It is based on stable-isotope dilution and possesses inherent accuracy and precision. Quantitative analysis is achieved by using commercially available homocystine-d(8) as an internal standard. Taking advantage of the high sensitivity and specificity, approaches involving LC-ESI-MS/MS require less laborious sample preparation, no derivatization and produce reliable results.</p>","PeriodicalId":520661,"journal":{"name":"Journal of chromatography. B, Analytical technologies in the biomedical and life sciences","volume":" ","pages":"3292-9"},"PeriodicalIF":3.0,"publicationDate":"2009-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jchromb.2009.07.024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40008590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Capillary electrophoresis in the evaluation of aminothiols in body fluids.","authors":"Filippo Carlucci,&nbsp;Antonella Tabucchi","doi":"10.1016/j.jchromb.2009.07.030","DOIUrl":"https://doi.org/10.1016/j.jchromb.2009.07.030","url":null,"abstract":"<p><p>Thiols play a fundamental role in cell biology, biochemistry and pharmacology. Altered thiol levels in body fluids are linked to specific pathological conditions. Glutathione is the most abundant intracellular low-molecular-mass thiol, playing an essential role in protecting cells from toxic species; other relevant thiol-containing compounds are homocysteine (Hcy), cysteine (Cys), cysteinylglycine (CysGly). Plasma aminothiols can be bound to proteins but they also occur free in the disulfide (symmetrical and mixed) and in the reduced forms. The simultaneous determination of these aminothiols, their precursor and metabolites is a useful tool in studying oxidative stress, metabolic and redox regulation. Many capillary electrophoresis methods have been proposed for this purpose, the aim of the present review is to support researchers in the choice of suitable methods for the determination of thiols in body fluids evaluating the different approaches and technologies proposed from the literature.</p>","PeriodicalId":520661,"journal":{"name":"Journal of chromatography. B, Analytical technologies in the biomedical and life sciences","volume":" ","pages":"3347-57"},"PeriodicalIF":3.0,"publicationDate":"2009-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jchromb.2009.07.030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40008591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPLC analysis of human erythrocytic glutathione forms using OPA and N-acetyl-cysteine ethyl ester: evidence for nitrite-induced GSH oxidation to GSSG.","authors":"Jan Thomas Michaelsen,&nbsp;Sabine Dehnert,&nbsp;Daniela Giustarini,&nbsp;Bibiana Beckmann,&nbsp;Dimitrios Tsikas","doi":"10.1016/j.jchromb.2009.06.043","DOIUrl":"https://doi.org/10.1016/j.jchromb.2009.06.043","url":null,"abstract":"<p><p>Glutathione exists in biological samples in the reduced form (GSH), as its disulfide (GSSG) and as a mixed disulfide (GSSR) with thiols (RSH). GSH is the most abundant low-molecular-mass thiol and plays important roles as a cofactor and as a main constituent of the intracellular redox status. Due to its own sulfhydryl (SH) group, GSH reacts readily with o-phthaldialdehyde (OPA) to form a highly stable and fluorescent isoindole derivative (GSH-OPA), which allows for sensitive and specific quantitative determination of GSH in biological systems by HPLC with fluorescence (FL) detection. In the present article we report on the utility of the novel, strongly disulfide bond-reducing thiol N-acetyl-cysteine ethyl ester (NACET) for the specific quantitative analysis of GSH and GSSG in the cytosol of red blood cells (RBC) as GSH-OPA derivative with FL (excitation/emission 338/458nm) or UV absorbance (338nm) detection. Unlike in aqueous solution, the derivatization of GSH in RBC cytosol yielded two closely related derivatives in the absence of NACET and only the GSH-OPA derivative in the presence of NACET. The HPLC method was optimized and validated for human RBC and applied to measure GSH and GSSG in RBC of healthy subjects. Basal GSH and GSSG concentrations were determined to be 2340+/-350microM and 11.4+/-3.2microM, respectively, in RBC of 12 healthy young volunteers (aged 23-38 years). The method was also applied to study the effects of nitrite on the glutathione status in intact and lysed human RBC. Nitrite at mM-concentrations caused instantaneous and considerable GSSG formation in lysed but much less pronounced in intact RBC. GSH externally added to lysed RBC inhibited nitrite-induced methemoglobin formation. Our findings suggest that nitric oxide/nitrite-related consumption rate of GSH, and presumably that of NADH and NADPH, could be of the order of 600micromol/day in RBC of healthy subjects.</p>","PeriodicalId":520661,"journal":{"name":"Journal of chromatography. B, Analytical technologies in the biomedical and life sciences","volume":" ","pages":"3405-17"},"PeriodicalIF":3.0,"publicationDate":"2009-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jchromb.2009.06.043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40021565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proceedings of the 33rd Annual Meeting of the Japanese Society for Biomedical Mass Spectroscopy. September 25-26, 2008. Tokyo, Japan.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":520661,"journal":{"name":"Journal of chromatography. B, Analytical technologies in the biomedical and life sciences","volume":" ","pages":"2599-661"},"PeriodicalIF":3.0,"publicationDate":"2009-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40042125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methodologies for the transfer of analytical methods: a review.","authors":"E Rozet,&nbsp;W Dewé,&nbsp;E Ziemons,&nbsp;A Bouklouze,&nbsp;B Boulanger,&nbsp;Ph Hubert","doi":"10.1016/j.jchromb.2008.12.049","DOIUrl":"https://doi.org/10.1016/j.jchromb.2008.12.049","url":null,"abstract":"<p><p>The transfer of a method from a laboratory to a production site is an important step in the development cycle of new pharmaceutical products. Method transfers are increasingly implemented due to the economical pressure coming from the rationalization of production sites, analytical subcontracting and fusion of pharmaceutical groups. However, no official guidance regarding study design, data analysis, or decision procedures is present neither in FDA documents nor in ICH documents for method transfers. The experiments performed in such a transfer and the methodology used to accept or reject it should be fitted for purpose. In order to provide to analysts a global view of the problematic of analytical method transfer, this paper reviews the documentation available in the scientific literature about the design of transfer studies and the required sample size. Special focus is also made on the statistical methodologies available for decision making with particular emphasis on risk management. Examples of transfer of pharmaceutical, bio-pharmaceutical and biological methods published in the literature are reviewed in order to illustrate the various possibilities among the strategies for methods transfer.</p>","PeriodicalId":520661,"journal":{"name":"Journal of chromatography. B, Analytical technologies in the biomedical and life sciences","volume":" ","pages":"2214-23"},"PeriodicalIF":3.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jchromb.2008.12.049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39992202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fatal intoxications by acenocoumarol, phenprocoumon and warfarin: method validation in blood using the total error approach.","authors":"Raphaël Denooz,&nbsp;Zoénabo Douamba,&nbsp;Corinne Charlier","doi":"10.1016/j.jchromb.2008.11.049","DOIUrl":"https://doi.org/10.1016/j.jchromb.2008.11.049","url":null,"abstract":"<p><p>A simple high-performance liquid chromatography (HPLC) method with ultraviolet (UV) detection has been developed and validated for simultaneous identification and quantification of three antivitamin K drugs (acenocoumarol, warfarin and phenprocoumon) in whole blood. The aim of this development was to propose an analytical technique adapted to the situations of forensic toxicology, i.e. intoxication with massive anticoagulant doses, when the usual coagulation tests could not be used. The blood sample, after spiked with prazepam as an internal standard (IS), was submitted to a liquid-liquid extraction (LLE) prior to HPLC analysis. A chromatographic separation was achieved on a C8 Symmetry column with a mobile phase consisting of an acetonitrile and phosphate buffer (pH 3.8) mixture in a gradient mode. Detection was carried out at a wavelength between 200 and 400 nm. This method has been validated with the concept of total error as decision criterion. Trueness ranged from 99.1% to 105.0% and precision was good with RSD between 1.3% and 6.7%. Consequently, this rapid and simple chromatographic technique is well adapted to focus intoxications with most important coumarinic drugs available on pharmaceutical market and is now routinely used in our laboratory for forensic \"general unknown\" screening.</p>","PeriodicalId":520661,"journal":{"name":"Journal of chromatography. B, Analytical technologies in the biomedical and life sciences","volume":" ","pages":"2344-8"},"PeriodicalIF":3.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jchromb.2008.11.049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39992203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}