Drug discoveries & therapeutics最新文献

{"title":"Changing epidemiology of respiratory pathogens since 2020: Shenzhen case study and global perspectives.","authors":"Chun Chen, Houming Liu, Jiaye Liu, Peifen Chen, Hongzhou Lu","doi":"10.5582/ddt.2025.01108","DOIUrl":"https://doi.org/10.5582/ddt.2025.01108","url":null,"abstract":"<p><p>The coronavirus disease 2019 (COVID-19) pandemic fundamentally disrupted global respiratory virus epidemiology through widespread non-pharmaceutical interventions. Analysis of a tertiary hospital in Shenzhen (2021-2024) reveals profound alterations in seasonal patterns. Influenza A exhibited multiple atypical peaks including summer circulation, while influenza B showed delayed resurgence with sustained winter activity. Respiratory syncytial virus demonstrated altered seasonality with substantial warm-season transmission replacing traditional winter patterns. World Health Organization (WHO) global surveillance confirmed parallel worldwide trends. Influenza activity collapsed dramatically in 2021 before resurging with irregular timing and unprecedented intensity through 2025. Respiratory syncytial virus exhibited off-season epidemics across multiple regions before gradually re-establishing modified seasonal patterns at elevated baseline levels. These epidemiological shifts resulted from immunity gaps created by reduced viral exposure, staggered lifting of pandemic restrictions across regions, and viral competition dynamics. Emerging technologies including AI-driven prediction models, expanded wastewater surveillance systems, and universal vaccine development offer promising approaches for managing future respiratory disease dynamics in this evolving post-pandemic landscape.</p>","PeriodicalId":520606,"journal":{"name":"Drug discoveries & therapeutics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145351291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification and characterization of a novel antioxidant protein from Arca subcrenata Lischke.","authors":"Qing Yan, Hui Shi, Yue Gao, Chunlei Li, Lin Dong, Jianhua Zhu, Rongmin Yu","doi":"10.5582/ddt.2025.01053","DOIUrl":"https://doi.org/10.5582/ddt.2025.01053","url":null,"abstract":"<p><p>A novel protein, G1H2GC2, was isolated from Arca subcrenata Lischke using homogenization and ammonium sulfate precipitation, and further purified by several column chromatography techniques including diethyl-aminoethanol (DEAE) Sepharose Fast Flow anion exchange, gel filtration chromatography (Sephadex G-25), Phenyl Sepharose CL-4B hydrophobic chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC). The purity of G1H2GC2 was over 97% in RP-HPLC, and its high purity was further verified by the appearance of a single band on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The protein content of G1H2GC2 was found to be over 99% by Bradford assay. The molecular weight was determined as 25.6 kDa by electrospray ionization-mass spectrometry (ESI-MS/MS). The isoelectric point of G1H2GC2 was measured to be 7.71 by isoelectric focusing-polyacrylamide gel electrophoresis (IEF-PAGE). Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS/MS) was employed to detect and identify the protein by mass fingerprinting coupled with fragmentation patterns. No matched protein was found, confirming that G1H2GC2 was a novel protein. An attempt was made to detect the N-terminal amino acid sequence of G1H2GC2 by Edman degradation, but the sequence of G1H2GC2 was found to be blocked. The scavenging percentage of G1H2GC2 at 15 mg/mL against 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS^•+) was 52.84%. The median effective concentration (EC₅₀) value of G1H2GC2 against ABTS^•+ was 17.67 mg/mL. The results showed that G1H2GC2 might be developed as a potential food additive agent.</p>","PeriodicalId":520606,"journal":{"name":"Drug discoveries & therapeutics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145351305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing gene-targeting efficiency of Agrobacterium tumefaciens-mediated transformation and electroporation in the pathogenic fungus Trichosporon asahii JCM2466.","authors":"Yasuhiko Matsumoto, Mei Nakayama, Yuta Shimizu, Sachi Koganesawa, Hiromi Kanai, Wakako Hayashi, Toma Matsuo, Tsuyoshi Yamada, Takashi Sugita","doi":"10.5582/ddt.2025.01084","DOIUrl":"https://doi.org/10.5582/ddt.2025.01084","url":null,"abstract":"<p><p>Trichosporon asahii is a pathogenic fungus that causes severe deep-seated fungal infections in neutropenic patients. Ku70, a key component of the non-homologous end-joining (NHEJ) pathway involved in the repair of DNA double-strand breaks, influences gene-targeting efficiency in T. asahii MPU129 strain using electroporation, a gene transfer method. Although phenotypic traits such as morphology and biofilm formation vary among T. asahii strains, the impact of different gene transfer methods on gene-targeting efficiency remains poorly characterized. In this study, we compared the gene-targeting efficiency of Agrobacterium tumefaciens-mediated transformation (ATMT) and electroporation. In T. asahii JCM2466 (CBS2479), a strain with high hyphal-forming ability, the ku70 gene-deficient mutant exhibited a higher gene-targeting efficiency via ATMT than the wild-type strain when generating a cnb1 gene-deficient mutant. The cnb1 gene encodes the β-subunit of calcineurin. In contrast, in the ku70 gene-deficient background of T. asahii JCM2466, cnb1-deficient mutants could not be generated by electroporation. The gene-targeting efficiencies of ATMT and electroporation in the ku70 gene-deficient mutant of T. asahii JCM2466 were 18% and 0%, respectively. The cnb1 gene-deficient mutants exhibited sensitivity to high temperature and several stress-inducing compounds. These results suggest that ATMT is a suitable gene transfer method for generating gene-deficient mutants in the ku70-deficient T. asahii JCM2466 background. Therefore, the choice of gene transfer method should be carefully tailored to the genetic background and phenotypic characteristics of each T. asahii strain.</p>","PeriodicalId":520606,"journal":{"name":"Drug discoveries & therapeutics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145331392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solid phase extraction and high-performance liquid chromatographic determination of lazertinib in human plasma.","authors":"Yoshito Gando, Takeo Yasu","doi":"10.5582/ddt.2025.01077","DOIUrl":"https://doi.org/10.5582/ddt.2025.01077","url":null,"abstract":"<p><p>Lazertinib is a novel third-generation tyrosine kinase inhibitor (TKI) developed for the treatment of epidermal growth factor receptor (EGFR) mutant non-small cell lung cancer (NSCLC). Reports on previous EGFR-TKIs have detailed significant associations between blood drug concentration and efficacy. In addition, significantly elevated blood concentrations of lazertinib have been observed in Asians compared to Caucasians, suggesting the influence of interethnic variability. In this study, we developed and validated a method to determine lazertinib concentrations in human plasma for therapeutic drug monitoring (TDM). Lazertinib and its internal standard, sotorasib, were extracted by solid-phase extraction using an Oasis hydrophilic lipophilic balance cartridge. Chromatographic separation was performed on a reversed-phase column with 0.5% KH₂PO₄ (pH 4.5) and acetonitrile (52:48, v/v) as the mobile phases in an isocratic elution mode with a flow rate of 1.0 mL/min. The detection wavelength was 296 nm. The calibration curves were linear in the range of 25-2,000 ng/mL, with a coefficient of determination (r²) of 0.9997. The accuracy and precision of all validation experiments were within the criteria set by the Food and Drug Administration guidelines. This study represents the first development and validation of a method for quantifying lazertinib in human plasma. This study is expected to facilitate the widespread use of TDM in studies on lazertinib.</p>","PeriodicalId":520606,"journal":{"name":"Drug discoveries & therapeutics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145331409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TRPC6 expression is negatively correlated with breast cancer progression.","authors":"Yifei Ma, Guoning Li, Haoyang Yu, Baoke Wang, Zhihan Diao, Yuan Du, Hongbo Wang, Huiping Liu","doi":"10.5582/ddt.2025.01082","DOIUrl":"https://doi.org/10.5582/ddt.2025.01082","url":null,"abstract":"<p><p>The transient receptor potential channel 6 (TRPC6), a Ca²⁺-permeable, non-selective cation channel, is a potential drug target widely expressed in most human tissues. However, the role of TRPC6 in breast cancer remains largely unknown. We comprehensively investigated the relationships between TRPC6 and breast cancer using online databases, including TIMER, cBioPortal, UALCAN, the Kaplan-Meier plotter, the Human Protein Atlas, STRING, and the LinkedOmics, to evaluate the prognostic value of TRPC6. We also tentatively explored the effects of TRPC6 modulation on proliferation and metastasis potential in several breast cancer cell lines by MTT assay, colony formation assay, flow cytometry, and scratch wound healing assay. The TCGA data showed lower TRPC6 gene expression in breast tumours versus normal breast tissues. The Human Protein Atlas revealed that the TRPC6 protein was generally expressed at low levels in both normal and breast carcinoma tissue. The Kaplan-Meier plotter showed that the low TRPC6 level is correlated with worse relapse-free survival probability in breast cancer. Moreover, the TRPC6 gene was linked to immune infiltration in breast cancer, and this relationship affected the prognosis of breast cancer. In contrast to published researches, we found that TRPC6 expression was negatively correlated with breast cancer cell proliferation and metastasis abilities, suggesting TRPC6 as a promising prognostic biomarker for breast cancer, where low expression of TRPC6 was related to worse prognoses. This research raises the necessity of rethinking the mechanism perspective and targetability of TRPC6 in breast cancer, which warrants further investigation.</p>","PeriodicalId":520606,"journal":{"name":"Drug discoveries & therapeutics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145305079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indicators of a moist wound environment and care to maintain a moist environment for early healing: A scoping review.","authors":"Mao Kunimitsu, Karin Izumi, Yume Kawamizu, Kenshin Takezawa, Kanon Nishida, Hitomi Honda, Yuri Maeda, Rena Yamauchi, Risa Yoshida, Yukari Nakajima, Amika Yamada, Kanae Mukai, Makoto Oe","doi":"10.5582/ddt.2025.01083","DOIUrl":"https://doi.org/10.5582/ddt.2025.01083","url":null,"abstract":"<p><p>Although maintaining a moist environment is important for wound healing, excess moisture can delay wound healing. Furthermore, recommended assessment methods for moist environments in the latest consensus guidelines are subjective. This scoping review aimed to map indicators to assess the moist environment in a wound and summarize the effectiveness of wound healing care in maintaining a moist environment, as assessed using these indicators. We searched four databases, the Medical Literature Analysis and Retrieval System On-Line, Cumulative Index to Nursing and Allied Health Literature, PubMed, and the Japan Medical Abstracts Society database, using a combination of chronic wound- and moisture-related terms. Independent researchers screening the articles based on the inclusion criteria and extracting relevant data afterward. After screening 2,727 articles, eight met the inclusion criteria: original articles/case studies, studies involving patients with chronic wounds, studies on care to maintain a moist wound environment, and studies assessing wound healing and moist wound environment. The articles included in this review demonstrated that dressings that absorb or hydrate exudates and care decisions based on the moisture content of the wound surface were effective care strategies for wound healing. Additional research is required to determine the best objective indicator for the assessment of a moist wound environment because despite the numerous existing indicators, only few reference values for healing have been reported.</p>","PeriodicalId":520606,"journal":{"name":"Drug discoveries & therapeutics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145305119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tapeworm infection incidence in rural Japan points to a common environmental source of infection.","authors":"Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Yu Suzuki, Daisuke Akaneya, Mika Moriya, Taichi Adachi, Yoshikazu Okuma, Daisuke Ishizawa, Hitoshi Ishikawa, Kazuhiko Miyanaga, Longzhu Cui, Kazunori Moriya, Hirotake Mori, Naveen Kumar Devanga Ragupathi, Yoshitaka Shimotai, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Talerngsak Kanjanabuch, Paul G Higgins, Tetsuji Aoyagi, Anthony Kicic, Sam Trowsdale, Parichart Hongsing, Aisha Khatib, Kenji Shibuya, Shuichi Abe, Hiroshi Hamamoto","doi":"10.5582/ddt.2025.01070","DOIUrl":"https://doi.org/10.5582/ddt.2025.01070","url":null,"abstract":"<p><p>Dibothriocephalus nihonkaiensis is a zoonotic tapeworm transmitted to humans through consumption of raw or undercooked fish or wild meat. Between 2022 and 2023, Yamagata Prefecture reported an increase in cases compared with 2017-2021, when none were observed. We conducted a clinical and environmental investigation to clarify infection sources. Four confirmed and one suspected patient were identified, all presenting with gastrointestinal symptoms. Exposures were linked to raw cherry salmon (Oncorhynchus masou) in three cases and undercooked bear meat in one case. Praziquantel treatment (10-20 mg/kg) was effective, with eight worms (76-210 cm) recovered. Environmental surveillance detected D. nihonkaiensis in 33.3% of bear feces (20/60) and 21.8% of wild fish samples (17/78). Phylogenetic analysis showed close genetic relatedness among human, bear, and fish isolates, indicating a shared transmission cycle. These findings confirm zoonotic transmission of D. nihonkaiensis in Yamagata and highlight the need for food safety awareness and environmental monitoring.</p>","PeriodicalId":520606,"journal":{"name":"Drug discoveries & therapeutics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145305085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Mycobacterium avium complex activities of streptcytosine analogs from a marine actinomycete as nucleoside antibiotics.","authors":"Natsuki Oshima, Akiho Yagi, Hiroyuki Yamazaki, Ryuji Uchida","doi":"10.5582/ddt.2025.01052","DOIUrl":"10.5582/ddt.2025.01052","url":null,"abstract":"<p><p>To examine the potential of nucleoside antibiotics as therapeutic agents against Mycobacterium avium complex (MAC), the in vitro and in vivo anti-MAC activities of streptcytosine A (1), plicacetin (2), and bamicetin (3) derived from a marine actinomycete were evaluated. Compounds 1-3 exhibited antimicrobial activities against M. avium and M. intracellulare, with minimum inhibitory concentration values of 4.0 and 16 μg/mL, respectively, as assessed by the microdilution method. In silkworm infection models of M. avium and M. intracellulare, these compounds also exhibited therapeutic efficacies at lower doses than clarithromycin, with 50% effective doses of between 7.6 and 28 μg/larva·g, and no toxicity was observed. A pharmacokinetic analysis further revealed elimination half-lives of 3.0, 2.3, and 5.1 hours, respectively, in the silkworm hemolymph. These results suggest the potential of 1-3 as lead candidates for the development of potent anti-MAC drugs.</p>","PeriodicalId":520606,"journal":{"name":"Drug discoveries & therapeutics","volume":" ","pages":"262-266"},"PeriodicalIF":0.0,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144986207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Telisotuzumab vedotin: The first-in-class c-Met-targeted antibody-drug conjugate granted FDA accelerated approval for treatment of non-squamous non-small cell lung cancer (NSCLC).","authors":"Chenru Zhao, Daoran Lu, Jianjun Gao","doi":"10.5582/ddt.2025.01058","DOIUrl":"10.5582/ddt.2025.01058","url":null,"abstract":"<p><p>Telisotuzumab vedotin represents a clinically important antibody-drug conjugate (ADC) that received accelerated approval from the US Food and Drug Administration in May 2025, establishing it as the first-in-class targeted therapy for adult patients with immunohistochemistry (IHC)-confirmed high-c-Met, EGFR wild-type, locally advanced or metastatic non-squamous non-small cell lung cancer (NSCLC). Its mechanism of action relies on precise targeting of the c-Met protein receptor, followed by internalization and release of the potent cytotoxic payload monomethyl auristatin E (MMAE) to eradicate tumor cells. The pivotal phase II LUMINOSITY trial demonstrated that the high c-Met overexpressing group had an overall response rate (ORR) of 34.6%. This therapeutic agent addresses a critical unmet need within a molecularly defined NSCLC subpopulation, marking a substantial advancement in c-Met-targeted oncology. The regulatory authorization and clinical use of telisotuzumab vedotin may significantly advance precision medicine for NSCLC, though an ongoing phase III trial will further confirm its efficacy and safety and determine its eligibility for full regulatory approval in the future.</p>","PeriodicalId":520606,"journal":{"name":"Drug discoveries & therapeutics","volume":" ","pages":"275-276"},"PeriodicalIF":0.0,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144629304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of electrospun composite biomaterial and porcine small intestine submucosa patch in open inguinal herniorrhaphy: A prospective, randomized, single-blind, controlled, multicenter, 72-month clinical study.","authors":"Zhiying Qiu, Liang Fang, Shaojie Li, Jianxiong Tang, Yun Pang, Jing Wang, Jing Zhou, Ling Wang, Lin Chen","doi":"10.5582/ddt.2025.01066","DOIUrl":"10.5582/ddt.2025.01066","url":null,"abstract":"<p><p>The aim of this study was to compare postoperative complications and long-term clinical efficacy of electrospun composite biomaterial and porcine small intestine submucosa (SIS) patch in open inguinal herniorrhaphy. A prospective, randomized, single-blind, controlled, multicenter study was used to select 172 patients with inguinal hernia who met the inclusion criteria in 3 hospitals. All participants were randomly assigned (1:1) and treated by open inguinal herniorrhaphy. The experimental group used electrospun composite biomaterial patch and the control group used SIS patch. Complications were assessed by ultrasonic volume auto-scan (UVAS) at 6, 33 and 72 months after operation. The results showed that 53 cases were found to be positive by UVAS, including 20 cases of encapsulated effusion (12.74%), 11 cases of soft tissue edema (6.40%), 4 cases of recurrence (3.01%), 2 cases of testicular ischemic atrophy (1.50%), 7 cases of testicular hydrocele (5.26%), 6 cases of varicocele (4.51%), 2 cases of spermatic cord cyst (1.27%) and 1 case of epididymal head cyst (0.64%). At 33-month follow-up, 2 cases recurred in the control group (2/79, 2.53%) while none in the experimental group (0/78) (the 95% CI difference in effective rates between the two groups was -0.93% to 6.00%, within the preset non-degradation range of Δ10%). At 72-month follow-up, there were 4 cases of recurrence in the control group (4/59, 6.78%), while none in the experimental group (0/60) (the lower limit of 95% CI difference in effective rates between the two groups was 0.27% > 0). The main clinical efficacy of the experimental group was better than that of the control group. There was no statistical difference in the secondary efficacy between the two groups. The postoperative safety evaluation effect of the two groups was the same. Long-term follow-up with UVAS after operation showed that the main clinical efficacy of electrospun composite biomaterial applied in this procedure was superior to that of SIS patch, and there was no significant difference in the secondary efficacy between the two biological patches.</p>","PeriodicalId":520606,"journal":{"name":"Drug discoveries & therapeutics","volume":" ","pages":"245-252"},"PeriodicalIF":0.0,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144986247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}