Diagnostics (Basel, Switzerland)最新文献

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When a Rapid Accurate Diagnosis Changes Therapeutic Approach: Recognizing Acute Abdominal Pain with Ascites as a Possible Presentation of Systemic Lupus Erythematosus. 当快速准确的诊断改变了治疗方法:认识到急性腹痛并腹水可能是系统性红斑狼疮的表现。
IF 3.6
Diagnostics (Basel, Switzerland) Pub Date : 2022-10-27 DOI: 10.3390/diagnostics12112605
Szu-Cheng Huang, Yi-Ling Chan, Hao-Tsai Cheng, Zhong Ning Leonard Goh, Yon-Cheong Wong, Chen-Ken Seak, Joanna Chen-Yeen Seak, Chih-Huang Li, Hsien-Yi Chen, Chen-June Seak
{"title":"When a Rapid Accurate Diagnosis Changes Therapeutic Approach: Recognizing Acute Abdominal Pain with Ascites as a Possible Presentation of Systemic Lupus Erythematosus.","authors":"Szu-Cheng Huang,&nbsp;Yi-Ling Chan,&nbsp;Hao-Tsai Cheng,&nbsp;Zhong Ning Leonard Goh,&nbsp;Yon-Cheong Wong,&nbsp;Chen-Ken Seak,&nbsp;Joanna Chen-Yeen Seak,&nbsp;Chih-Huang Li,&nbsp;Hsien-Yi Chen,&nbsp;Chen-June Seak","doi":"10.3390/diagnostics12112605","DOIUrl":"https://doi.org/10.3390/diagnostics12112605","url":null,"abstract":"<p><p>Systemic lupus erythematosus (SLE) is a chronic, multi-organ autoimmune disease which rarely presents with peritoneal involvement. As such, its diagnosis in the emergency department (ED) based on a clinical presentation of gastrointestinal symptoms is extremely challenging. Yet, reaching such a diagnosis in the ED is crucial for avoiding unnecessary surgical intervention and initiating early glucocorticoid therapy to maximise patient outcomes. Here, we report a case of newly diagnosed SLE in a 28-year-old lady who presented atypically and unusually with abdominal pain and ascites. She required extensive but methodical investigations, and was eventually diagnosed with lupus mesenteric vasculitis with underlying newly diagnosed SLE in the ED. The patient was promptly treated with methylprednisolone resulting in marked clinical improvement. Emergency physicians should be mindful of abdominal pain with ascites as an extremely rare but important clinical presentation of SLE. Early diagnosis and commencement of glucocorticoid therapy in these patients are crucial in halting disease progression and averting the need for surgical intervention.</p>","PeriodicalId":520604,"journal":{"name":"Diagnostics (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40459596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vantera Mediated Quantification of Urine Citrate and Creatinine: A New Technology to Assess Risk of Nephrolithiasis. Vantera介导的尿柠檬酸盐和肌酐定量:评估肾结石风险的新技术。
IF 3.6
Diagnostics (Basel, Switzerland) Pub Date : 2022-10-27 DOI: 10.3390/diagnostics12112606
Steven P Matyus, Justyna Wolak-Dinsmore, Erwin Garcia, Randolph M Young, Margery A Connelly
{"title":"Vantera Mediated Quantification of Urine Citrate and Creatinine: A New Technology to Assess Risk of Nephrolithiasis.","authors":"Steven P Matyus,&nbsp;Justyna Wolak-Dinsmore,&nbsp;Erwin Garcia,&nbsp;Randolph M Young,&nbsp;Margery A Connelly","doi":"10.3390/diagnostics12112606","DOIUrl":"https://doi.org/10.3390/diagnostics12112606","url":null,"abstract":"<p><p>Urine citrate is often used to identify patients at risk of recurrent nephrolithiasis or kidney stones. A high-throughput assay was developed to measure urine citrate and creatinine on the Vantera<sup>®</sup> Clinical Analyzer, a nuclear magnetic resonance (NMR) instrument designed for the clinical laboratory. Assay performance was evaluated and comparisons between the NMR and chemistry results were conducted. Linearity was demonstrated over a wide range of concentrations for citrate (6 and 2040 mg/L) and creatinine (2.8 and 1308 mg/dL). Intra-and inter-assay precision (%CV) ranged from 0.9 to 3.7% for citrate and 0.4 to 2.1% for creatinine. The correlation coefficients for the comparison between NMR and chemistry results were 0.98 (Y = 1.00X + 5.0) for citrate and 0.96 (Y = 0.968X + 0.97) for creatinine. The reference intervals for both analytes were confirmed. Ten endogenous and exogenous substances were tested and none were found to interfere with the assay results. In conclusion, the newly developed high-throughput NMR assay exhibited robust performance and generated results comparable to the currently utilized chemistry tests, thereby providing an alternative means to simultaneously quantify urine citrate and creatinine for clinical and research use. Furthermore, the NMR assay does not exhibit the same interference limitations as the chemistry tests and it enables multiplexing with other urine metabolite assays which saves time and costs.</p>","PeriodicalId":520604,"journal":{"name":"Diagnostics (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40459597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Accuracy of Noninvasive Diagnostic Tests for the Detection of Significant and Advanced Fibrosis Stages in Nonalcoholic Fatty Liver Disease: A Systematic Literature Review of the US Studies. 非酒精性脂肪性肝病中重要和晚期纤维化阶段的非侵入性诊断测试的准确性:美国研究的系统文献综述
IF 3.6
Diagnostics (Basel, Switzerland) Pub Date : 2022-10-27 DOI: 10.3390/diagnostics12112608
Dhaval Gosalia, Vlad Ratziu, Filip Stanicic, Djurdja Vukicevic, Vladimir Zah, Nadege Gunn, Dina Halegoua-DeMarzio, Tram Tran
{"title":"Accuracy of Noninvasive Diagnostic Tests for the Detection of Significant and Advanced Fibrosis Stages in Nonalcoholic Fatty Liver Disease: A Systematic Literature Review of the US Studies.","authors":"Dhaval Gosalia,&nbsp;Vlad Ratziu,&nbsp;Filip Stanicic,&nbsp;Djurdja Vukicevic,&nbsp;Vladimir Zah,&nbsp;Nadege Gunn,&nbsp;Dina Halegoua-DeMarzio,&nbsp;Tram Tran","doi":"10.3390/diagnostics12112608","DOIUrl":"https://doi.org/10.3390/diagnostics12112608","url":null,"abstract":"<p><strong>Background: </strong>The purpose of this systematic literature review (SLR) was to evaluate the accuracy of noninvasive diagnostic tools in detecting significant or advanced (F2/F3) fibrosis among patients with nonalcoholic fatty liver (NAFL) in the US healthcare context.</p><p><strong>Methods: </strong>The SLR was conducted in PubMed and Web of Science, with an additional hand search of public domains and citations, in line with the PRISMA statement. The study included US-based original research on diagnostic test sensitivity, specificity and accuracy.</p><p><strong>Results: </strong>Twenty studies were included in qualitative evidence synthesis. Imaging techniques with the highest diagnostic accuracy in F2/F3 detection and differentiation were magnetic resonance elastography and vibration-controlled transient elastography. The most promising standard blood biomarkers were NAFLD fibrosis score and FIB-4. The novel diagnostic tools showed good overall accuracy, particularly a score composed of body mass index, GGT, 25-OH-vitamin D, and platelet count. The novel approaches in liver fibrosis detection successfully combine imaging techniques and blood biomarkers.</p><p><strong>Conclusions: </strong>While noninvasive techniques could overcome some limitations of liver biopsy, a tool that would provide a sufficiently sensitive and reliable estimate of changes in fibrosis development and regression is still missing.</p>","PeriodicalId":520604,"journal":{"name":"Diagnostics (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40479748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Mental Strategies in a P300-BCI: Visuomotor Transformation Is an Option. P300-BCI的心理策略:视觉运动转换是一种选择。
IF 3.6
Diagnostics (Basel, Switzerland) Pub Date : 2022-10-27 DOI: 10.3390/diagnostics12112607
Nikolay Syrov, Lev Yakovlev, Varvara Nikolaeva, Alexander Kaplan, Mikhail Lebedev
{"title":"Mental Strategies in a P300-BCI: Visuomotor Transformation Is an Option.","authors":"Nikolay Syrov,&nbsp;Lev Yakovlev,&nbsp;Varvara Nikolaeva,&nbsp;Alexander Kaplan,&nbsp;Mikhail Lebedev","doi":"10.3390/diagnostics12112607","DOIUrl":"https://doi.org/10.3390/diagnostics12112607","url":null,"abstract":"<p><p>Currently, P300-BCIs are mostly used for spelling tasks, where the number of commands is equal to the number of stimuli that evoke event-related potentials (ERPs). Increasing this number slows down the BCI operation because each stimulus has to be presented several times for better classification. Furthermore, P300 spellers typically do not utilize potentially useful imagery-based approaches, such as the motor imagery successfully practiced in motor rehabilitation. Here, we tested a P300-BCI with a motor-imagery component. In this BCI, the number of commands was increased by adding mental strategies instead of increasing the number of targets. Our BCI had only two stimuli and four commands. The subjects either counted target appearances mentally or imagined hand movements toward the targets. In this design, the motor-imagery paradigm enacted a visuomotor transformation known to engage cortical and subcortical networks participating in motor control. The operation of these networks suffers in neurological conditions such as stroke, so we view this BCI as a potential tool for the rehabilitation of patients. As an initial step toward the development of this clinical method, sixteen healthy participants were tested. Consistent with our expectation that mental strategies would result in distinct EEG activities, ERPs were different depending on whether subjects counted stimuli or imagined movements. These differences were especially clear in the late ERP components localized in the frontal and centro-parietal regions. We conclude that (1) the P300 paradigm is suitable for enacting visuomotor transformations and (2) P300-based BCIs with multiple mental strategies could be used in applications where the number of possible outputs needs to be increased while keeping the number of targets constant. As such, our approach adds to both the development of versatile BCIs and clinical approaches to rehabilitation.</p>","PeriodicalId":520604,"journal":{"name":"Diagnostics (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40479749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Graphene-Based Sensor for the Detection of Cortisol for Stress Level Monitoring and Diagnostics. 用于压力水平监测和诊断的基于石墨烯的皮质醇检测传感器。
IF 3.6
Diagnostics (Basel, Switzerland) Pub Date : 2022-10-26 DOI: 10.3390/diagnostics12112593
Alexei Zubarev, Marina Cuzminschi, Ana-Maria Iordache, Stefan-Marian Iordache, Constantin Rizea, Cristiana E A Grigorescu, Carmen Giuglea
{"title":"Graphene-Based Sensor for the Detection of Cortisol for Stress Level Monitoring and Diagnostics.","authors":"Alexei Zubarev,&nbsp;Marina Cuzminschi,&nbsp;Ana-Maria Iordache,&nbsp;Stefan-Marian Iordache,&nbsp;Constantin Rizea,&nbsp;Cristiana E A Grigorescu,&nbsp;Carmen Giuglea","doi":"10.3390/diagnostics12112593","DOIUrl":"https://doi.org/10.3390/diagnostics12112593","url":null,"abstract":"<p><p>In this work, we study the sensing properties of multi-layer graphene combined with pyrrole in order to elaborate low-cost, high-sensitive material for cortisol detection. Graphene nanoplatelets and pyrrole were dispersed in a solution containing 1M HNO<sub>3</sub> by using a powerful ultrasound probe for 10 min, then centrifuged for 30 min at 4000 rpm; polymerization was performed by cyclic voltammetry. The graphene-pyrrole composite was tested to ultra-low levels of cortisol in artificial saliva, consistent to the levels excreted in human salivary samples. The composite was further investigated by Raman spectroscopy and we modeled the interaction between the sensitive layer and cortisol using MarvinBeans software. It shows a good sensitivity for salivary values of cortisol cyclic voltammetry being able to detect a level down to 0.5 ng/mL cortisol.</p>","PeriodicalId":520604,"journal":{"name":"Diagnostics (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2022-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40457260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Energy, Waves, and Forces in Bilateral Fracture of the Femoral Necks: Two Case Presentations and Updated Critical Review. 双侧股骨颈骨折的能量、波和力:两个病例报告和最新的评论。
IF 3.6
Diagnostics (Basel, Switzerland) Pub Date : 2022-10-26 DOI: 10.3390/diagnostics12112592
Cosmin Constantin Baciu
{"title":"Energy, Waves, and Forces in Bilateral Fracture of the Femoral Necks: Two Case Presentations and Updated Critical Review.","authors":"Cosmin Constantin Baciu","doi":"10.3390/diagnostics12112592","DOIUrl":"https://doi.org/10.3390/diagnostics12112592","url":null,"abstract":"<p><p>Two case reports and an updated critical review on bilateral fractures of the femoral neck are presented. Bilateral fractures of the femoral neck have been investigated for at least 80 years and are treated as rare cases. The primary cause is usually considered an external shock; however, aside from high energy shocks (e.g., falling and impact with hard, rigid surfaces, traffic accidents, etc.) the underlying causes of femoral neck frailty have not yet been fully understood. Although not exhaustive, the review spans cases reported as early as 1944 and compares their conclusions in line with medicine developments at the time of the reports until present. The discussion is perhaps controversial at times; it brings to the fore the energy balance between shock waves and stress waves. The two cases reported here add to the review, one highlighting the biomechanics, and the other supporting more recent findings on metabolic disorders, which ultimately lead to enhanced frailty of the femoral neck. Investigation of the fractures has been performed with X-ray radiographs, MRI, and CT, with a follow up using a Doppler US to check blood flow in the lower zone of the limbs. The second case was investigated both for fractures and metabolic diseases, e.g., type I diabetes and kidney failure (dialysis). In Case 1 the second fracture was not observable at the time of admission, and therefore two surgery operations were performed at seven days interval. Taperloc Complete prostheses (Zimmer Biomet) were applied. Case 2 suffered a second fracture in the right hip in the segment above the knee and required better fixation with cables. Despite this, she returned one month later with a new crack in the femur. Case 1 is a typical case of wear consequences on the biomechanics of the hill pad-tibia-femur-femoral neck system, where tension of the neck occurred due to a stress wave rather than a shock wave. This can be proven by the absence of a second fracture from the images first acquired, the only evidence being pain and walking difficulty. Case 2 shows that metabolic diseases can dramatically enhance the frequency of bilateral femoral neck fractures.</p>","PeriodicalId":520604,"journal":{"name":"Diagnostics (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2022-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689340/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40457261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An Improved Detection Algorithm for Ischemic Stroke NCCT Based on YOLOv5. 基于YOLOv5改进的缺血性脑卒中NCCT检测算法
IF 3.6
Diagnostics (Basel, Switzerland) Pub Date : 2022-10-26 DOI: 10.3390/diagnostics12112591
Lifeng Zhang, Hongyan Cui, Anming Hu, Jiadong Li, Yidi Tang, Roy Elmer Welsch
{"title":"An Improved Detection Algorithm for Ischemic Stroke NCCT Based on YOLOv5.","authors":"Lifeng Zhang,&nbsp;Hongyan Cui,&nbsp;Anming Hu,&nbsp;Jiadong Li,&nbsp;Yidi Tang,&nbsp;Roy Elmer Welsch","doi":"10.3390/diagnostics12112591","DOIUrl":"https://doi.org/10.3390/diagnostics12112591","url":null,"abstract":"<p><p>Cerebral stroke (CS) is a heterogeneous syndrome caused by multiple disease mechanisms. Ischemic stroke (IS) is a subtype of CS that causes a disruption of cerebral blood flow with subsequent tissue damage. Noncontrast computer tomography (NCCT) is one of the most important IS detection methods. It is difficult to select the features of IS CT within computational image analysis. In this paper, we propose AC-YOLOv5, which is an improved detection algorithm for IS. The algorithm amplifies the features of IS via an NCCT image based on adaptive local region contrast enhancement, which then detects the region of interest via YOLOv5, which is one of the best detection algorithms at present. The proposed algorithm was tested on two datasets, and seven control group experiments were added, including popular detection algorithms at present and other detection algorithms based on image enhancement. The experimental results show that the proposed algorithm has a high accuracy (94.1% and 91.7%) and recall (85.3% and 88.6%) rate; the recall result is especially notable. This proves the excellent performance of the accuracy, robustness, and generalizability of the algorithm.</p>","PeriodicalId":520604,"journal":{"name":"Diagnostics (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2022-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40457259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A Deep Learning Algorithm for Radiographic Measurements of the Hip in Adults-A Reliability and Agreement Study. 用于成人髋关节放射测量的深度学习算法-可靠性和一致性研究。
IF 3.6
Diagnostics (Basel, Switzerland) Pub Date : 2022-10-26 DOI: 10.3390/diagnostics12112597
Janni Jensen, Ole Graumann, Søren Overgaard, Oke Gerke, Michael Lundemann, Martin Haagen Haubro, Claus Varnum, Lene Bak, Janne Rasmussen, Lone B Olsen, Benjamin S B Rasmussen
{"title":"A Deep Learning Algorithm for Radiographic Measurements of the Hip in Adults-A Reliability and Agreement Study.","authors":"Janni Jensen,&nbsp;Ole Graumann,&nbsp;Søren Overgaard,&nbsp;Oke Gerke,&nbsp;Michael Lundemann,&nbsp;Martin Haagen Haubro,&nbsp;Claus Varnum,&nbsp;Lene Bak,&nbsp;Janne Rasmussen,&nbsp;Lone B Olsen,&nbsp;Benjamin S B Rasmussen","doi":"10.3390/diagnostics12112597","DOIUrl":"https://doi.org/10.3390/diagnostics12112597","url":null,"abstract":"<p><p>Hip dysplasia (HD) is a frequent cause of hip pain in skeletally mature patients and may lead to osteoarthritis (OA). An accurate and early diagnosis may postpone, reduce or even prevent the onset of OA and ultimately hip arthroplasty at a young age. The overall aim of this study was to assess the reliability of an algorithm, designed to read pelvic anterior-posterior (AP) radiographs and to estimate the agreement between the algorithm and human readers for measuring (i) lateral center edge angle of Wiberg (LCEA) and (ii) Acetabular index angle (AIA). The algorithm was based on deep-learning models developed using a modified U-net architecture and ResNet 34. The newly developed algorithm was found to be highly reliable when identifying the anatomical landmarks used for measuring LCEA and AIA in pelvic radiographs, thus offering highly consistent measurement outputs. The study showed that manual identification of the same landmarks made by five specialist readers were subject to variance and the level of agreement between the algorithm and human readers was consequently poor with mean measured differences from 0.37 to 9.56° for right LCEA measurements. The algorithm displayed the highest agreement with the senior orthopedic surgeon. With further development, the algorithm may be a good alternative to humans when screening for HD.</p>","PeriodicalId":520604,"journal":{"name":"Diagnostics (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2022-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40457264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Risk Factors for Post-Traumatic Stress Disorder after Childbirth: A Systematic Review. 分娩后创伤后应激障碍的危险因素:系统综述。
IF 3.6
Diagnostics (Basel, Switzerland) Pub Date : 2022-10-26 DOI: 10.3390/diagnostics12112598
Ijlas El Founti Khsim, Mirella Martínez Rodríguez, Blanca Riquelme Gallego, Rafael A Caparros-Gonzalez, Carmen Amezcua-Prieto
{"title":"Risk Factors for Post-Traumatic Stress Disorder after Childbirth: A Systematic Review.","authors":"Ijlas El Founti Khsim,&nbsp;Mirella Martínez Rodríguez,&nbsp;Blanca Riquelme Gallego,&nbsp;Rafael A Caparros-Gonzalez,&nbsp;Carmen Amezcua-Prieto","doi":"10.3390/diagnostics12112598","DOIUrl":"https://doi.org/10.3390/diagnostics12112598","url":null,"abstract":"<p><strong>Background: </strong>Post-traumatic stress disorder (PTSD) after birth has generated a growing interest in recent years. Although some risk factors associated with PTSD have been studied, information is still scarce to date on risk factors associated with PTSD. This systematic review aims to identify risk factors associated with the diagnosis of PTSD after childbirth.</p><p><strong>Methods: </strong>We searched on PubMed, Web of Science and SCOPUS databases, from inception to May 2022. Quality assessment of the articles was performed using the Newcastle-Ottawa Quality Assessment (\"NOQAS\") scale. This systematic review was performed according to the PRISMA guidelines. Inclusion criteria were women with age ≥18 years; articles in English or Spanish; articles focused on physical, social, psychological, medical-obstetric, and environmental risk factors.</p><p><strong>Results: </strong>A total of <i>n</i> = 17,675 women were included among the studies in this systematic review. The main risk factors associated with PTSD after birth were obstetric interventions and obstetric violence such as emergency caesarean section or a non-compliant birth plan, a previous mental illness, having suffered from of a traumatic event or depression and/or anxiety, and having poor social support throughout pregnancy and/or during birth.</p><p><strong>Conclusions: </strong>Obstetric interventions, obstetric violence, experiencing a traumatic event or depression and/or anxiety, and a previous mental illness are factors associated with the diagnosis of PTSD after birth. Protective factors are multiparity, adherence to the mother's birth plan and skin-to-skin contact.</p>","PeriodicalId":520604,"journal":{"name":"Diagnostics (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2022-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40457265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Comparative Study of DNA Extraction Methods for the PCR Detection of Intestinal Parasites in Human Stool Samples. 人粪便中肠道寄生虫DNA提取方法的比较研究。
IF 3.6
Diagnostics (Basel, Switzerland) Pub Date : 2022-10-25 DOI: 10.3390/diagnostics12112588
Siriporn Srirungruang, Buraya Mahajindawong, Panachai Nimitpanya, Uthaitip Bunkasem, Pattama Ayuyoe, Surang Nuchprayoon, Vivornpun Sanprasert
{"title":"Comparative Study of DNA Extraction Methods for the PCR Detection of Intestinal Parasites in Human Stool Samples.","authors":"Siriporn Srirungruang,&nbsp;Buraya Mahajindawong,&nbsp;Panachai Nimitpanya,&nbsp;Uthaitip Bunkasem,&nbsp;Pattama Ayuyoe,&nbsp;Surang Nuchprayoon,&nbsp;Vivornpun Sanprasert","doi":"10.3390/diagnostics12112588","DOIUrl":"https://doi.org/10.3390/diagnostics12112588","url":null,"abstract":"<p><p>Stool samples typically contain PCR inhibitors; however, helminths are difficult to lyse and can cause false-negative PCR results. We assessed the effective methods for extracting DNA from different kinds of intestinal parasites. We compared the most common DNA extraction methods from stool samples, including the phenol-chloroform technique with or without a bead-beating step (P and PB), a QIAamp Fast DNA Stool Mini Kit (Q), and a QIAamp PowerFecal Pro DNA Kit (QB). Genomic DNA was extracted from 85 stool samples collected from patients infected with <i>Blastocystis</i> sp., <i>Ascaris lumbricoides, Trichuris trichiura,</i> hookworm, and <i>Strongyloides stercoralis</i>. DNA quantity and DNA quality were evaluated via spectrophotometry, and DNA integrity was assessed by PCR. We found that P and PB provided higher DNA yields (~4 times) than when using Q and QB. However, P showed the lowest detection rate of PCR (8.2%), wherein only <i>S. stercoralis</i> (7 out of 20 samples) was detected. QB showed the highest detection rate of PCR (61.2%). After plasmid spikes, only 5 samples by QB were negative while 60 samples by P were still negative. Remarkably, QB could extract DNA from all the groups of parasites that we tested. These results indicate that QB is the most effective DNA extraction method for the diagnosis and monitoring of intestinal parasites via PCR.</p>","PeriodicalId":520604,"journal":{"name":"Diagnostics (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689707/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40466613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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