Arhiv za higijenu rada i toksikologiju最新文献

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Noise exposure patterns and health risk assessment among nurses in a Chinese paediatric intensive care unit. 中国儿科重症监护室护士噪声暴露模式及健康风险评估
IF 1.6
Arhiv za higijenu rada i toksikologiju Pub Date : 2026-03-30 eCollection Date: 2026-03-01 DOI: 10.2478/aiht-2026-77-4077
Yan Liu, Junlin Tan
{"title":"Noise exposure patterns and health risk assessment among nurses in a Chinese paediatric intensive care unit.","authors":"Yan Liu, Junlin Tan","doi":"10.2478/aiht-2026-77-4077","DOIUrl":"10.2478/aiht-2026-77-4077","url":null,"abstract":"<p><p>Starting from the hypothesis that occupational noise exposure among paediatric intensive care unit (PICU) nurses at our hospital exceeds the recommended health guidelines and is associated with adverse non-auditory health outcomes, the aim of this cross-sectional study was to quantify personal noise exposure patterns and assess correlations with perceived stress, sleep quality, and hearing handicap in a convenience sample of 60 registered nurses wearing personal noise dosimeters over a 12-hour shift. The participants also completed a survey including the 10-item Perceived Stress Scale (PSS-10), the Pittsburgh Sleep Quality Index (PSQI), and the Hearing Handicap Inventory for Adults (HHIA). The main finding was that the cohort's mean 8-hour time-weighted average (L<sub>Aeq,8 h</sub>) exposure to noise was 78.5±4.2 dBA, and that all measured shifts exceeded the World Health Organization (WHO) hospital guidelines. Peak noise levels (L<sub>Cpeak</sub>) frequently surpassed 115 dBC. Most nurses (N=39) were classified as \"poor sleepers\" (PSQI>5). Significant positive correlations were found between L<sub>Aeq,8 h</sub> and PSS-10 scores (r=0.45, p<0.001) and global PSQI scores (r=0.52, p<0.001). For every 1 dBA increase in L<sub>Aeq,8 h</sub>, the odds of being a poor sleeper increased by 25 % (odds ratio 1.25; 95 % CI: 1.05-1.48). Even though this is a \"snapshot study\", it clearly indicates that PICU noise bears significant occupational risks of high stress and poor sleep, which warrants immediate intervention. Further research should focus on longitudinal measurements to get a better idea of noise exposure in healthcare environments, PICUs in particular.</p>","PeriodicalId":520565,"journal":{"name":"Arhiv za higijenu rada i toksikologiju","volume":"77 1","pages":"39-48"},"PeriodicalIF":1.6,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13061083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147629768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Indoor air bacterial and fungal burden in the environment of an atopic child: implications for elevated urine mycotoxin levels. 特应性儿童环境中的室内空气细菌和真菌负荷:尿真菌毒素水平升高的影响。
IF 1.6
Arhiv za higijenu rada i toksikologiju Pub Date : 2026-03-30 eCollection Date: 2026-03-01 DOI: 10.2478/aiht-2026-77-4024
Ivona Majić, Adela Krivohlavek, Elvira Kovač Andrić, Ranka Godec
{"title":"Indoor air bacterial and fungal burden in the environment of an atopic child: implications for elevated urine mycotoxin levels.","authors":"Ivona Majić, Adela Krivohlavek, Elvira Kovač Andrić, Ranka Godec","doi":"10.2478/aiht-2026-77-4024","DOIUrl":"10.2478/aiht-2026-77-4024","url":null,"abstract":"<p><p>Here we present a case of an atopic boy from Zagreb, Croatia, whose elevated urine mycotoxin levels prompted us investigate whether they were associated with microbial indoor air burden in the child's primary and music school and family home. The music school had been water-damaged / mould-infested but had been repaired by the time of our measurements. We also measured urine mycotoxin in one healthy child who attended the same elementary school and in all household members of the atopic boy. The results showed no microbial contamination at any of the measurement locations, with bacterial concentrations ranging from 172 to over 570 CFU/m<sup>3</sup> of air and fungal concentrations between 67 and 82 CFU/m<sup>3</sup>, all determined only in the music school classroom. The dominant species isolated in the music school were <i>Aspergillus fumigatus</i> (55 CFU/m<sup>3</sup>) and <i>Penicillium verrucosum</i> (24 CFU/m<sup>3</sup>), both potential mycotoxin producers. Considering that only the atopic boy had elevated urine mycotoxin levels, we believe that mould in the music school cannot be ruled out as the source of exposure. In fact, we believe our case illustrates that safety thresholds for mould exposure can vary and may be much lower for immunocompromised or sensitive individuals.</p>","PeriodicalId":520565,"journal":{"name":"Arhiv za higijenu rada i toksikologiju","volume":"77 1","pages":"65-72"},"PeriodicalIF":1.6,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13061072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147629732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Coasting effect of n-hexane-induced neuropathy evidenced by electroneuromyography and clinical symptom scales: a 12-month follow-up study. 神经肌电图和临床症状量表证明正己烷诱导的神经病变的滑行效应:一项12个月的随访研究。
IF 1.6
Arhiv za higijenu rada i toksikologiju Pub Date : 2026-03-30 eCollection Date: 2026-03-01 DOI: 10.2478/aiht-2026-77-4034
Yusuf Koçak, Uğur Kulu, Tuba Demirer Çeker, Orhan Sümbül, Dürdane Aksoy, Betül Çevik
{"title":"Coasting effect of <i>n</i>-hexane-induced neuropathy evidenced by electroneuromyography and clinical symptom scales: a 12-month follow-up study.","authors":"Yusuf Koçak, Uğur Kulu, Tuba Demirer Çeker, Orhan Sümbül, Dürdane Aksoy, Betül Çevik","doi":"10.2478/aiht-2026-77-4034","DOIUrl":"10.2478/aiht-2026-77-4034","url":null,"abstract":"<p><p>To the best of our knowledge, no study so far has investigated the coasting effect of neuropathy caused by occupational exposure to <i>n</i>-hexane through more detailed clinical evaluations than nerve conduction measurements, such that would also include pain assessment and functional status. The aim of our study was therefore to see if our measurements, which include all three elements, would support the coasting effect and reveal associations between electrophysiological measurements and clinical symptom assessments over a 12-month follow-up. Our study included eighteen patients working in the same shoe factory who were diagnosed with occupational neuropathy most likely caused by exposure to <i>n</i>-hexane. After identification of occupational exposure to high, yet unspecified VOC levels by local health authorities, production was suspended and all patients removed from further exposure. All underwent detailed neurological examination, including electroneuromyography (EMG) and the assessment of self-rated pain severity, symptoms, and functionality in everyday activities. All clinical, laboratory, and electrophysiological assessments were performed at baseline and at months 4 and 12 of follow-up. The patients presented with different degrees of numbness, muscle weakness, and sensory disturbances. EMG measurements and clinical scales at month 4 revealed significant worsening from the baseline (p<0.05), and the assessments at month 12 a significant improvement from month 4 (p<0.05), which confirms the coasting effect. Significant correlations were found between baseline motor conduction velocities and subsequent functional outcomes, and between selected sensory parameters and pain severity. Regardless of its limitations, our study points to the real-life consequences of exposure to harmful VOCs in poorly controlled shoe factory environments and establishes the coasting effect through more than one diagnostic parameter. From a preventive perspective, our findings suggest that early electrophysiological evaluation, together with simple and validated clinical scales, may help identify workers at risk of developing neuropathy before irreversible functional impairments occur. Such an approach could inform timely exposure control, medical surveillance, and occupational interventions in high-risk settings.</p>","PeriodicalId":520565,"journal":{"name":"Arhiv za higijenu rada i toksikologiju","volume":"77 1","pages":"28-38"},"PeriodicalIF":1.6,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13061073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147629723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Forsythoside A attenuates metabolic dysfunction in type 2 diabetic mice by inhibiting the MAPK and activating the Nrf2 signalling pathways. 连翘苷A通过抑制MAPK和激活Nrf2信号通路来减轻2型糖尿病小鼠的代谢功能障碍。
IF 1.6
Arhiv za higijenu rada i toksikologiju Pub Date : 2026-03-30 eCollection Date: 2026-03-01 DOI: 10.2478/aiht-2026-77-4026
Mengxian Shu, Chunhui Xiang
{"title":"Forsythoside A attenuates metabolic dysfunction in type 2 diabetic mice by inhibiting the MAPK and activating the Nrf2 signalling pathways.","authors":"Mengxian Shu, Chunhui Xiang","doi":"10.2478/aiht-2026-77-4026","DOIUrl":"10.2478/aiht-2026-77-4026","url":null,"abstract":"<p><p>Forsythoside A, a natural phenylethanoid glycoside extracted from the weeping forsythia (<i>Forsythia suspensa</i>), exhibits a wide range of pharmacological activity, including antibacterial, hepatoprotective, antioxidant, neuroprotective, antiviral, and anti-inflammatory. The aim of this study was to determine its anti-hyperglycaemic and antioxidative effects in a diabetic mouse model (created by administering high-fat diet alongside successive low doses of streptozotocin) by measuring fasting blood glucose levels, body weight, food and water intake, oxidative stress, and histopathological changes. Diabetic mice received either forsythoside A (30 or 60 mg/kg bw) or metformin (150 mg/kg) as standard type 2 diabetes medication for comparison. After four weeks of administration, forsythoside A significantly increased body weight and reduced food and water intake at both doses, while the higher, 60 mg/kg dose also significantly reduced fasting blood glucose and had a similar effect on all these parameters as metformin. The higher, 60 mg/kg dose also had similar antioxidative effects as metformin in lowering malondialdehyde (MDA) and reactive oxygen species (ROS) and in elevating the antioxidant superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) levels. Moreover, at 60 mg/kg forsythoside A attenuated lipid accumulation in diabetic mice by elevating high-density lipoprotein cholesterol (HDL-C) and lowering total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C), showing comparable effect to metformin. Similar improvements were observed by histopathological changes in the liver. Forsythoside A also lowered insulin levels in diabetic mice by up-regulating p-IRS-1 and inhibited the mitogen-activated protein kinase (MAPK) pathway by lowering the expressions of the p-p38 and p-JNK proteins. At the same time, it promoted the Nrf2 pathway by increasing Nrf2 and HO-1 expressions relative to untreated diabetic mice. In conclusion, forsythoside A demonstrated therapeutic effects akin to those of 150 mg/kg metformin and may be a promising candidate for clinical application.</p>","PeriodicalId":520565,"journal":{"name":"Arhiv za higijenu rada i toksikologiju","volume":"77 1","pages":"21-27"},"PeriodicalIF":1.6,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13061084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147629726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Autonomic echoes of student life: a mixed-method inquiry with meta-inferential integration of autonomic function, lifestyle narratives, and health behaviours among medical students. 学生生活的自主回声:医学生自主功能、生活方式叙述和健康行为元推理整合的混合方法调查。
IF 1.6
Arhiv za higijenu rada i toksikologiju Pub Date : 2026-03-30 eCollection Date: 2026-03-01 DOI: 10.2478/aiht-2026-77-4057
Dharani Bhaskaran, Suba Anagppan, Logeshwari Vijayagopal, Abeetha Subramanian, Chitra Mourali, Daniel Dev Merlin
{"title":"Autonomic echoes of student life: a mixed-method inquiry with meta-inferential integration of autonomic function, lifestyle narratives, and health behaviours among medical students.","authors":"Dharani Bhaskaran, Suba Anagppan, Logeshwari Vijayagopal, Abeetha Subramanian, Chitra Mourali, Daniel Dev Merlin","doi":"10.2478/aiht-2026-77-4057","DOIUrl":"10.2478/aiht-2026-77-4057","url":null,"abstract":"<p><p>Medical students face intense academic and lifestyle transitions that can affect their physiological health and well-being. This mixed-method study explores the interplay between the autonomic nervous system activity, measured via heart rate variability (HRV) and lifestyle behaviours, measured with a 20-item questionnaire (LBQ-20) in 90 first-year medical students in South India. We also gathered narrative responses to understand subjective health perceptions and stress adaptation. HRV parameters, calculated individually for each participant and summarised using group-level means, indicate moderate overall autonomic variability (SDNN of 42.76±18.20 ms and RMSSD of 28.37±13.75 ms), while the mean LBQ-20 score of 9.46±3.16 indicates moderate overall adherence to health-promoting behaviours across the cohort. The correlation between the two is weak and non-significant. Thematic analysis of narratives highlight rigid academic routines, stress, poor sleep, and dietary challenges as major barriers to health. This study suggests that medical student well-being is shaped not only by personal lifestyle but also by systemic and psychological stressors.</p>","PeriodicalId":520565,"journal":{"name":"Arhiv za higijenu rada i toksikologiju","volume":"77 1","pages":"49-56"},"PeriodicalIF":1.6,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13061075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147629676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Public awareness of microplastic pollution among residents of a western Turkish province and how it is reflected in demographics and behavioural practices. 土耳其西部某省居民对微塑料污染的公众意识及其在人口统计和行为实践中的反映。
IF 1.6
Arhiv za higijenu rada i toksikologiju Pub Date : 2026-03-30 eCollection Date: 2026-03-01 DOI: 10.2478/aiht-2026-77-4030
Ömer Faruk Tekin, İnci Arıkan, Muhammet Ali Bölükbaş
{"title":"Public awareness of microplastic pollution among residents of a western Turkish province and how it is reflected in demographics and behavioural practices.","authors":"Ömer Faruk Tekin, İnci Arıkan, Muhammet Ali Bölükbaş","doi":"10.2478/aiht-2026-77-4030","DOIUrl":"10.2478/aiht-2026-77-4030","url":null,"abstract":"<p><p>This cross-sectional study aimed to evaluate the public awareness of and attitudes toward microplastic pollution in the Kütahya Province of Turkey between September and December 2024 and included 406 users of regional family healthcare centres. Data were collected with a specifically developed questionnaire combining sociodemographic information and self-reported knowledge, attitudes, and behaviour related to the microplastics issue and with our Microplastic Pollution Awareness Scale (MPAS), against which we compared the answers to the first questionnaire. MPAS scores were significantly higher among women than men, persons under the age of 40, unmarried participants, university graduates (5.1 times higher than in those with lower education), and residents of developed regions (2.3 times higher than among residents of underdeveloped or developing regions). Overall, participants show pretty good awareness of microplastic pollution. Future prospective studies should cover all ages, but more importantly, develop effective intervention programmes aimed at changing public behaviour.</p>","PeriodicalId":520565,"journal":{"name":"Arhiv za higijenu rada i toksikologiju","volume":"77 1","pages":"57-64"},"PeriodicalIF":1.6,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13061086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147629771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structure-guided modification of a lead cholinesterase-targeting oxime to improve peripheral site binding. 以胆碱酯酶为靶点的铅肟的结构导向修饰以改善外周位点的结合。
IF 1.6
Arhiv za higijenu rada i toksikologiju Pub Date : 2026-03-30 eCollection Date: 2026-03-01 DOI: 10.2478/aiht-2026-77-4078
Nikola Maraković, Nicolas Probst, Tena Čadež, Antonio Zandona
{"title":"Structure-guided modification of a lead cholinesterase-targeting oxime to improve peripheral site binding.","authors":"Nikola Maraković, Nicolas Probst, Tena Čadež, Antonio Zandona","doi":"10.2478/aiht-2026-77-4078","DOIUrl":"10.2478/aiht-2026-77-4078","url":null,"abstract":"<p><p>Based on our previous study on <i>N‑</i>substituted 2-hydroxyiminoacetamides as cholinesterase-targeting ligands, which highlighted 2-hydroxyimino-<i>N</i>-(3-(4-((2-methyl-<i>1H</i>-imidazol-1-yl)methyl)-<i>1H</i>-1,2,3-triazol-1-yl)-1-phenylpropyl)acetamide as lead compound because of its inhibition potency, we extended this group of compounds by introducing a new, bulkier peripheral anionic site (PAS)-binding substituent - 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline to achieve an orientation of its three modular elements that would comply with our initial design: the benzyl unit to occupy the choline binding region, the bulky substituent to stabilise binding at the peripheral site, and the 2-hydroxyiminoacetamide moiety to bind at the catalytic serine residue. The obtained <i>N</i>-(3-(4-((6,7-dimethoxy-3,4-dihydroisoquinolin-2(<i>1H</i>)-yl)methyl)-<i>1H</i>-1,2,3-triazol-1-yl)-1-phenylpropyl)-2-(hydroxyimino)acetamide inhibited both human acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) non-competitively in a low micromolar range. Molecular docking studies predicted high stability of the 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline group at PAS and of the 2-hydroxyiminoacetamide moiety and the benzyl group inside the catalytic site of BChE, while the closest orientation of the three modular elements to our initial design was predicted for AChE, though different orientations were also predicted. Cytotoxicity was observed in the HepG2 and HEK293 cell lines, but only at concentrations exceeding those used in the reversible inhibition assay. Our study confirms <i>N</i>-substituted 2-hydroxiiminoacetamides with three structural elements as low micromolar cholinesterase ligands, successfully extending the scope of PAS substituent to 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline. Beyond cholinesterase inhibition, our findings contribute to better understanding of the cytotoxic properties of constituting structural elements of the tested <i>N</i>-(3-(4-((6,7-dimethoxy-3,4-dihydroisoquinolin-2(<i>1H</i>)-yl)methyl)-<i>1H</i>-1,2,3-triazol-1-yl)-1-phenylpropyl)-2-(hydroxyimino)acetamide.</p>","PeriodicalId":520565,"journal":{"name":"Arhiv za higijenu rada i toksikologiju","volume":"77 1","pages":"1-8"},"PeriodicalIF":1.6,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13061080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147629737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chronic lymphatic leukaemia in a worker with a long-term occupational exposure to petroleum product mixtures: a case report. 长期职业性接触石油产品混合物的工人患慢性淋巴白血病1例报告。
IF 1.6
Arhiv za higijenu rada i toksikologiju Pub Date : 2026-03-30 eCollection Date: 2026-03-01 DOI: 10.2478/aiht-2026-77-4094
Diana Bućan, Ivan Bućan
{"title":"Chronic lymphatic leukaemia in a worker with a long-term occupational exposure to petroleum product mixtures: a case report.","authors":"Diana Bućan, Ivan Bućan","doi":"10.2478/aiht-2026-77-4094","DOIUrl":"10.2478/aiht-2026-77-4094","url":null,"abstract":"<p><p>Following a regular occupational health checkup, a 63-year-old worker employed at petrol station for thirty years was diagnosed with chronic lymphocytic leukaemia (CLL). The patient's occupational history revealed long-term daily exposure to petroleum-derived vapours. As part of a routine occupational health assessment, he underwent comprehensive laboratory testing, followed by further evaluation and treatment by a haematologist, including a myelogram, immunophenotyping of haemoblastosis, and bone marrow histopathology. Based on the completed diagnostic workup, the patient was diagnosed CLL with B-cell predominance. Due to the nature of the disease and job demands, the worker was declared permanently unfit for work at his current workplace. The diagnosis was recognised as an occupational disease by the Croatian Health Insurance Fund thanks to the expert opinion, issued by the occupational health specialist, who, after an extensive toxicological assessment and the exclusion of other possible causes, linked 30 years of occupational inhalation exposure to petroleum products containing benzene with the onset of this malignant disease. This case highlights systemic shortcomings in the assessment of occupational exposure to mixtures of petroleum products, inhalation of vapours, and potential dermal contact among petrol station workers, deficiencies in occupational safety measures, and the crucial role of occupational medicine specialists in the prevention, early detection, and risk management before disease progression, as well as in supporting the legal and occupational rights of affected workers.</p>","PeriodicalId":520565,"journal":{"name":"Arhiv za higijenu rada i toksikologiju","volume":"77 1","pages":"73-77"},"PeriodicalIF":1.6,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13061081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147629720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Potential of Bacillus paramycoides hydrolase to degrade propiconazole. 副芽孢杆菌水解酶降解丙环唑的潜力。
IF 1.6
Arhiv za higijenu rada i toksikologiju Pub Date : 2026-03-30 eCollection Date: 2026-03-01 DOI: 10.2478/aiht-2026-77-4001
Muhammad Naveed, Maida Salah Ud Din, Tariq Aziz, Rida Naveed, Daochen Zhu, Maha Alharbi, Ashwag Shami
{"title":"Potential of <i>Bacillus paramycoides</i> hydrolase to degrade propiconazole.","authors":"Muhammad Naveed, Maida Salah Ud Din, Tariq Aziz, Rida Naveed, Daochen Zhu, Maha Alharbi, Ashwag Shami","doi":"10.2478/aiht-2026-77-4001","DOIUrl":"10.2478/aiht-2026-77-4001","url":null,"abstract":"<p><p>Propiconazole is a widely used synthetic fungicide that has raised deep environmental and toxicological concerns due to its persistence and bioactivity. In this study, we investigated the potential of a facultative anaerobic, Gram-positive, rod-shaped bacterium, <i>Bacillus paramycoides</i> to degrade propiconazole by elucidating the functional role of its hydrolase enzyme <i>in silico</i>. The hydrolase was characterised with the FASTA sequence to determine its physicochemical properties, stability, and conserved functional domains. Homology modelling was performed and the predicted structure validated using a Ramachandran plot and ERRAT analysis, yielding an overall quality score of 93.6 %. Eleven propiconazole-related compounds, including parent molecules and degradation products (e.g. hispor, propiconazole TP1, propiconazole TP2, and propiconazole-d7) were retrieved from the PubChem database and subjected to molecular docking using PyRx. Docking analysis revealed stable enzyme-substrate interactions, with the highest binding affinity of -6.8 kcal/mol observed for native hydrolase complexes. Site-directed mutagenesis was subsequently performed, and mutant structures were evaluated for structural stability and functional integrity. The mutated hydrolase exhibited an improved binding affinity of -7.4 kcal/mol, indicating enhanced substrate interaction. Molecular dynamics simulations using the AMBER force field further confirmed the structural stability, binding consistency, and functional reliability of the enzyme-ligand complexes. Overall, these quantitative findings support the potential of <i>B. paramycoides</i> hydrolase as a stable, non-virulent, and efficient candidate for environmentally sustainable bioremediation of propiconazole, with relevance to environmental and occupational toxicology.</p>","PeriodicalId":520565,"journal":{"name":"Arhiv za higijenu rada i toksikologiju","volume":"77 1","pages":"9-20"},"PeriodicalIF":1.6,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13061085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147629681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Risks of radiation exposure to old military compasses with radioluminescent markings. 带有放射发光标记的旧军用罗盘的辐射暴露风险。
IF 1.6
Arhiv za higijenu rada i toksikologiju Pub Date : 2025-12-30 eCollection Date: 2025-12-01 DOI: 10.2478/aiht-2025-76-4038
Igor Miklavčić, Igor Lassinger, Vanja Radolić, Marina Poje Sovilj
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