Applied immunohistochemistry & molecular morphology : AIMM最新文献_第5页

Clinical and Economic Value of p16INK4a for the Differential Diagnosis of Morphologic Cervical Intraepithelial Neoplasia 2. p16INK4a在形态学宫颈上皮内瘤变鉴别诊断中的临床和经济价值

IF 1.6

Applied immunohistochemistry & molecular morphology : AIMM Pub Date : 2019-10-01 DOI: 10.1097/PAI.0000000000000674

Vanina S Fishkel, Fernando C Monge, Felicitas M von Petery, Karen E Tapper, Teresa M Peña, Florencia Torres, Fernando A Poletta, Jorge F Elgart, Alejandra Avagnina, Valeria Denninghoff

{"title":"Clinical and Economic Value of p16INK4a for the Differential Diagnosis of Morphologic Cervical Intraepithelial Neoplasia 2.","authors":"Vanina S Fishkel, Fernando C Monge, Felicitas M von Petery, Karen E Tapper, Teresa M Peña, Florencia Torres, Fernando A Poletta, Jorge F Elgart, Alejandra Avagnina, Valeria Denninghoff","doi":"10.1097/PAI.0000000000000674","DOIUrl":"https://doi.org/10.1097/PAI.0000000000000674","url":null,"abstract":"The detection of high-grade intraepithelial lesions requires highly sensitive and specific methods that allow more accurate diagnoses. This contributes to a proper management of preneoplastic lesions, thus avoiding overtreatment. The purpose of this study was to analyze the value of immunostaining for p16 in the morphologic assessment of cervical intraepithelial neoplasia 2 lesions, to help differentiate between low-grade (p16-negative) and high-grade (p16-positive) squamous intraepithelial lesions. The direct medical cost of the treatment of cervical intraepithelial neoplasia 2 morphologic lesions was estimated. A retrospective observational cross-sectional study was carried out. This study analyzed 46 patients treated with excisional procedures because of cervical intraepithelial neoplasia 2 lesions, using loop electrosurgical excision procedures. Immunostaining for the biomarker was performed. For the estimation of overtreatment, percentages (%) and their 95% confidence interval were calculated. Of the 41 patients analyzed, 32 (78%) showed overexpression of p16 and 9 (22%) were negative (95% confidence interval, 11%-38%). Mean follow-up was 2.9 years, using cervical cytology testing (Pap) and colposcopy. High-risk human papillomavirus DNA tests were performed in 83% of patients. These retrospective results reveal the need for larger biopsy samples, which would allow a more accurate prediction of lesion risk. Considering the cost of p16 staining, and assuming the proper management of the low-grade lesion, an average of US$919 could be saved for each patient with a p16-negative result, which represents a global direct cost reduction of 10%.","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"672-677"},"PeriodicalIF":1.6,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PAI.0000000000000674","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39965582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Endoglin is Highly Expressed in Human Mast Cells. 内啡肽在人肥大细胞中高表达。

IF 1.6

Applied immunohistochemistry & molecular morphology : AIMM Pub Date : 2019-09-01 DOI: 10.1097/PAI.0000000000000668

Suéllen Trentin Brum, Ana Paula Demasi, Rafael Fantelli Stelini, Maria Letícia Cintra, Vera Cavalcanti de Araujo, Andresa Borges Soares

{"title":"Endoglin is Highly Expressed in Human Mast Cells.","authors":"Suéllen Trentin Brum, Ana Paula Demasi, Rafael Fantelli Stelini, Maria Letícia Cintra, Vera Cavalcanti de Araujo, Andresa Borges Soares","doi":"10.1097/PAI.0000000000000668","DOIUrl":"https://doi.org/10.1097/PAI.0000000000000668","url":null,"abstract":"Endoglin, known to be expressed in proliferating vessels, is of worth when evaluating microvessel density as a prognostic factor in many types of malignancies, including some subtypes of leukemia cells. In childhood acute lymphoblastic leukemia, endoglin is associated with adverse outcome. In bone marrow, endoglin identifies the repopulating hematopoietic stem cells. Mast cells are a component of normal tissue and play an important role in the regulation of several processes, including inflammation and neoplasia. The aim of this study was to evaluate the use of endoglin as a biological marker of mast cells compared with the gold standard stains. We studied 15 specimens of neurofibroma, 9 of mastocytosis, and 6 of fibrous scar tissue through immunohistochemistry (for endoglin and mast cell tryptase) and histochemical staining using toluidine blue. Quantitative analysis of the cells was performed by counting 5 hotspots. The validity of endoglin as a mast cell marker was assessed by intraclass correlation coefficient. The Kruskal-Wallis test was used to compare mast cell count for each marker. A strong endoglin expression was found in the cytoplasmic granules of mast cells within the 3 groups. Similar results were observed with mast cell tryptase as well as toluidine blue. The intraclass correlation coefficient revealed that endoglin is a highly reliable biomarker of mast cells when compared with mast cell tryptase and toluidine blue. In conclusion, endoglin may assist in the diagnosis and pathogenesis study of various processes associated with mast cells. An endoglin-neutralizing treatment for solid cancers and leukemia could also affect mastocytes and the immunologic system.","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"613-617"},"PeriodicalIF":1.6,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PAI.0000000000000668","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39965572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Are Ski and SnoN Involved in the Tumorigenesis of Oral Squamous Cell Carcinoma Through Smad4? Ski和SnoN是否通过Smad4参与口腔鳞状细胞癌的发生?

IF 1.6

Applied immunohistochemistry & molecular morphology : AIMM Pub Date : 2019-09-01 DOI: 10.1097/PAI.0000000000000667

Mojgan Alaeddini, Shahroo Etemad-Moghadam

{"title":"Are Ski and SnoN Involved in the Tumorigenesis of Oral Squamous Cell Carcinoma Through Smad4?","authors":"Mojgan Alaeddini, Shahroo Etemad-Moghadam","doi":"10.1097/PAI.0000000000000667","DOIUrl":"https://doi.org/10.1097/PAI.0000000000000667","url":null,"abstract":"Transforming growth factor-β has been implicated in the tumorigenesis of oral squamous cell carcinoma (OSCC). Ski and SnoN are negative regulators of transforming growth factor-β/Smad pathway with both prooncogenic and antioncogenic functions in different cancers. The aim of this study was to assess the expression of Ski and SnoN in OSCC for the first time. Smad4 was also evaluated in these tumors. Clinical data on 61 primary OSCCs were gathered, and the specimens were subjected to immunohistochemical staining with monoclonal antibodies against SKI, SnoN, and Smad4 and scored semiquantitatively. Spearman rank, Fisher exact, and χ tests were used for statistical analysis, and P-value <0.05 was considered significant. Ski positivity and SnoN positivity were mostly cytoplasmic and found in 96.7% and 100% of the cases, respectively. Smad4 staining was low to negative in 65% of the specimens. No significant relationship was found either among the markers or between each of the proteins and the clinicopathologic data (P>0.05). According to our findings, Ski, SnoN, and Smad4 seem to play a role in OSCC oncogenesis, and we suggest that Ski and SnoN functions may take place independent of Smad4. Considering the dual and complex role of these proteins in tumorigenesis, further investigation to clarify the molecular pathways involved in their mode of action is suggested.","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"626-630"},"PeriodicalIF":1.6,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PAI.0000000000000667","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39965832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The Role of CD44 and RHAMM in Endometrial (Endometrioid Type) Cancer: An Immunohistochemical Study. CD44和RHAMM在子宫内膜(子宫内膜样型)癌中的作用:免疫组织化学研究。

IF 1.6

Applied immunohistochemistry & molecular morphology : AIMM Pub Date : 2019-09-01 DOI: 10.1097/PAI.0000000000000673

Marko Klarić, Herman Haller, Alemka Brnčić Fischer, Emina Babarović, Aleksandra Prijić, Senija Eminović

{"title":"The Role of CD44 and RHAMM in Endometrial (Endometrioid Type) Cancer: An Immunohistochemical Study.","authors":"Marko Klarić, Herman Haller, Alemka Brnčić Fischer, Emina Babarović, Aleksandra Prijić, Senija Eminović","doi":"10.1097/PAI.0000000000000673","DOIUrl":"https://doi.org/10.1097/PAI.0000000000000673","url":null,"abstract":"Hyaluronan controls cell migration, differentiation, and proliferation, and it is involved in tumor invasion. The extracellular matrix containing hyaluronan regulates cell behavior via cell surface receptors such as CD44 and receptor for hyaluronan-mediated motility (RHAMM, CD168). We investigated the expression of CD44 and RHAMM in tissue samples of endometrial cancer and the relation of their expression with clinicopathologic parameters of patients. In order to evaluate the value of CD44 and RHAMM as prognostic factors, we investigated the relation of their expression with patients' survival. Our results demonstrated a statistically significant correlation with the depth of myometrial invasion, lymphovascular invasion (LVSI), The International Federation of Gynecology and Obstetrics stage of disease, and, in the case of RHAMM expression, a significant correlation with histologic tumor grade as well. CD44 expression was present in the cell membrane in all cases, but in a proportion of tumors in the cytoplasm as well. In this group of patients, we noticed a significantly greater number of cases with deeper myometrial invasion and LVSI. Finally, we sorted out the group of tumors with simultaneous strong CD44 and strong RHAMM expression, and found a statistically significant correlation with the depth of myometrial invasion and LVSI. Using an univariate analysis, we demonstrated that, in our sample of patients, CD44 expression showed a statistically significant influence on patients' 5-year survival. However, using a multivariate Cox regression analysis, neither CD44 nor RHAMM confirmed themselves as independent prognostic factors.","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"606-612"},"PeriodicalIF":1.6,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PAI.0000000000000673","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40436757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Genetic Alterations of Epidermal Growth Factor Receptor in Glioblastoma: The Usefulness of Immunohistochemistry. 胶质母细胞瘤中表皮生长因子受体的遗传改变:免疫组织化学的作用。

IF 1.6

Applied immunohistochemistry & molecular morphology : AIMM Pub Date : 2019-09-01 DOI: 10.1097/PAI.0000000000000669

Minju Lee, So Young Kang, Yeon-Lim Suh

{"title":"Genetic Alterations of Epidermal Growth Factor Receptor in Glioblastoma: The Usefulness of Immunohistochemistry.","authors":"Minju Lee, So Young Kang, Yeon-Lim Suh","doi":"10.1097/PAI.0000000000000669","DOIUrl":"https://doi.org/10.1097/PAI.0000000000000669","url":null,"abstract":"Epidermal growth factor receptor (EGFR) amplification is one of the common alterations in IDH-wildtype glioblastoma. It is frequently associated with EGFRvIII mutation. To evaluate the correlation between EGFR overexpression, gene amplification, and EGFRvIII mutation, we performed immunohistochemical (IHC) analysis, fluorescence in situ hybridization by Vysis LSI EGFR/CEP7 dual color probe, and polymerase chain reaction studies in 76 patients diagnosed with glioblastomas (67 IDH-wildtype and 9 IDH-mutant). EGFR expression was scored ranging from 0 to 3+. Using formalin-fixed paraffin-embedded sections, real-time reverse transcription-polymerase chain reaction was carried out with primers specific for EGFRvIII and EGFR wildtype. In addition, we evaluated the impact of EGFR status on prognosis. EGFR gene amplifications and EGFRvIII mutations were identified in 30.3% and 15.5% of all cases, respectively. All the EGFR-amplified or EGFRvIII mutant cases were IDH-wildtype glioblastomas and tested positive with IHC. The sensitivity and specificity of EGFR IHC predicting EGFR gene amplification status were 100.0% and 46.5%, respectively. The EGFR-amplified cases tended to show more intense immunostaining (3+) in a considerable number of tumor cells (≥50%). Survival analyses of 37 IDH-wildtype glioblastoma patients revealed that none of the EGFR alterations significantly affected prognosis. EGFR IHC displayed high sensitivity and low specificity in predicting EGFR gene amplification, and interpretation of IHC results is a challenge. Therefore, EGFR IHC represents a possible screening tool for evaluation of EGFR gene amplification in clinical neuropathology, and both the intensity and proportion score facilitate interpretation of EGFR IHC.","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"589-598"},"PeriodicalIF":1.6,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PAI.0000000000000669","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40440924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Clinical Utility of Fluorescence In Situ Hybridization (FISH) for Deletion of Chromosome 3p in the Work-up of Renal Masses. 荧光原位杂交(FISH)检测染色体3p缺失在肾肿块检查中的临床应用。

IF 1.6

Applied immunohistochemistry & molecular morphology : AIMM Pub Date : 2019-08-01 DOI: 10.1097/PAI.0000000000000676

Carmen M Perrino, Jason A Orien, Jason G Tretter, Weiqiang Zhao, Debra L Zynger

{"title":"Clinical Utility of Fluorescence In Situ Hybridization (FISH) for Deletion of Chromosome 3p in the Work-up of Renal Masses.","authors":"Carmen M Perrino, Jason A Orien, Jason G Tretter, Weiqiang Zhao, Debra L Zynger","doi":"10.1097/PAI.0000000000000676","DOIUrl":"https://doi.org/10.1097/PAI.0000000000000676","url":null,"abstract":"Chromosome 3p deletion is a well-established genetic aberration in clear cell renal cell carcinoma (RCC). We aimed to evaluate the clinical utility of 3p fluorescence in situ hybridization (FISH) on formalin-fixed paraffin-embedded tissue in surgical pathology specimens. 3p:3q <0.8 was established as the cut-off for 3p loss. The 2015 Medicare allowable billing rates were used to estimate the cost. Over 2.5 years (2013 to 2015), 3p FISH was performed on 18 cases per year. Among tested cases, 70% (30/43) were nephrectomies and 30% (14/43) metastases. 3p loss was detected in 44% (19/43) of cases, with a higher rate of loss in radical compared with partial nephrectomies (71% vs. 15%; P=0.003). A definitive RCC subtype was assigned in 65% (28/43) of cases. More partial nephrectomies had a definitive subtype assigned, compared with radical nephrectomies (92% vs. 59%; P=0.04), possibly related to more high-grade, high-stage tumors in submitted radical nephrectomies. Tested nephrectomies were most commonly diagnosed as clear cell (41%) or clear cell papillary RCC (32%). Half of unclassifiable RCCs had 3p loss (53%, 8/15). Annual 3p FISH costs were $3446.64, with 79% of costs from ancillary studies attributable to immunostains. 3p FISH was performed infrequently in nephrectomy specimens and was not cost prohibitive. RCC cases that are unclassifiable by morphology and other ancillary tests, but which have 3p FISH deletion may merit a comment in the pathology report, raising the possibility of clear cell RCC, as the oncologic approach may be altered despite the lack of a definitive RCC subtype.","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"549-557"},"PeriodicalIF":1.6,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PAI.0000000000000676","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40540509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Epidermal Growth Factor Receptor-2 Promotes Invasion and Metastasis in Gastric Cancer by Activating Mitogen-activated Protein Kinase Signaling. 人表皮生长因子受体-2通过激活丝裂原激活的蛋白激酶信号促进胃癌的侵袭和转移。

IF 1.6

Applied immunohistochemistry & molecular morphology : AIMM Pub Date : 2019-08-01 DOI: 10.1097/PAI.0000000000000672

Feng Hou, Duan-Bo Shi, Yun-Qing Chen, Peng Gao

{"title":"Human Epidermal Growth Factor Receptor-2 Promotes Invasion and Metastasis in Gastric Cancer by Activating Mitogen-activated Protein Kinase Signaling.","authors":"Feng Hou, Duan-Bo Shi, Yun-Qing Chen, Peng Gao","doi":"10.1097/PAI.0000000000000672","DOIUrl":"https://doi.org/10.1097/PAI.0000000000000672","url":null,"abstract":"Increasing evidence supports an important role for the human epidermal growth factor receptor-2 (HER2) gene and mitogen-activated protein kinase (MAPK) signaling pathways in the progression of human cancers by enhancing cancer cell metastasis and proliferation. However, the relationship between HER2 and MAPK signaling pathways in gastric cancer (GC) remains unclear. In the present study, dual in situ hybridization was performed to detect HER2 gene amplification and reverse transcription-quantitative polymerase chain reaction was used to investigate the mRNA expression of members of the MAPK signaling pathway, including rapidly accelerated fibrosarcoma (RAF), extracellular regulated signal-activated kinase (ERK), p38, and c-Jun N-terminal kinase (JNK), in 112 primary GC tissue samples. The results revealed that 19/112 (17%) of tissue samples showed positive amplification of HER2, which was correlated with tumor invasion and metastasis. Upregulation of RAF, ERK, p38, and JNK was also observed in samples associated with metastasis. Moreover, the expression levels of RAF and ERK in samples with HER2 gene amplification were significantly increased compared with those without HER2 amplification. However, the expression levels of both p38 and JNK were not significantly correlated with HER2 gene amplification. Our results simultaneously showed the association between HER2 gene amplification and the expression levels of MAPK signaling pathway proteins and clinicopathologic characteristics in GC. These findings provide the basis for investigating the regulation of MAPK signaling pathways by HER2 and potential therapeutic targets for inhibiting metastasis and invasion in GC.","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"529-534"},"PeriodicalIF":1.6,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PAI.0000000000000672","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40548521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Gastrointestinal Stromal Tumor With Multiple Primary Tyrosine Kinase Mutations-Clinicopathologic and Molecular Characterization. 胃肠道间质瘤多发原发性酪氨酸激酶突变-临床病理和分子特征。

IF 1.6

Applied immunohistochemistry & molecular morphology : AIMM Pub Date : 2019-07-01 DOI: 10.1097/PAI.0000000000000660

Newton A C S Wong, Philippe Taniere, Shaun Walsh, Andrew Wallace, Daisuke Nonaka, Thomas Jones, David Gonzalez

{"title":"Gastrointestinal Stromal Tumor With Multiple Primary Tyrosine Kinase Mutations-Clinicopathologic and Molecular Characterization.","authors":"Newton A C S Wong, Philippe Taniere, Shaun Walsh, Andrew Wallace, Daisuke Nonaka, Thomas Jones, David Gonzalez","doi":"10.1097/PAI.0000000000000660","DOIUrl":"https://doi.org/10.1097/PAI.0000000000000660","url":null,"abstract":"A unique cohort of chemo-naive gastrointestinal stromal tumors (GISTs) with double-primary tyrosine kinase mutations was characterized particularly to determine whether coexistent mutations represent a single mutational event. Up to 2013, 4 UK centers reported 9 GISTs with 2 primary tyrosine kinase mutations. In each of 8 cases validated by next generation sequencing, both mutations were present in the same allele of the same exon (KIT exon 11 or 17, or PDGFRA exon 18). One case showed the second mutation only on some of the mutant alleles. Seven cases showed both mutations in all the reads, but in 2 cases, additional variants were found only in some reads. Clinicopathologic features of the 8 cases were similar to GISTs with single-primary mutations. When GIST genotyping rarely uncovers multiple tyrosine kinase variants in an exon, they occur in the same allele but are likely to represent separate mutational events and lack clinical significance.","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"461-465"},"PeriodicalIF":1.6,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PAI.0000000000000660","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39965558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The Royal College of Pathologists of Australasia Quality Assurance Program: Immunohistochemistry Breast Marker Audit Overview 2005-2015. 澳大利亚皇家病理学家学院质量保证计划:免疫组织化学乳房标记物审计概述2005-2015。

IF 1.6

Applied immunohistochemistry & molecular morphology : AIMM Pub Date : 2019-05-01 DOI: 10.1097/PAI.0000000000000621

Zenobia Ayesha Mohamed Haffajee, Beena Kumar, Glenn Francis, Martyn Peck, Tony Badrick

引用次数: 1

A Comprehensive Evaluation of Special AT-rich Sequence-binding Protein 2 (SATB2) Immunohistochemical Staining in Mucinous Tumors From Gastrointestinal and Nongastrointestinal Sites. 特异AT-rich Sequence-binding Protein 2 (SATB2)免疫组化染色在胃肠道和非胃肠道黏液瘤中的综合评价

IF 1.6

Applied immunohistochemistry & molecular morphology : AIMM Pub Date : 2019-05-01 DOI: 10.1097/PAI.0000000000000627

Benjamin D Ramos, Stefan Brettfeld, Ryan S Berry, Joshua K Routh, David R Martin, Joshua A Hanson

{"title":"A Comprehensive Evaluation of Special AT-rich Sequence-binding Protein 2 (SATB2) Immunohistochemical Staining in Mucinous Tumors From Gastrointestinal and Nongastrointestinal Sites.","authors":"Benjamin D Ramos, Stefan Brettfeld, Ryan S Berry, Joshua K Routh, David R Martin, Joshua A Hanson","doi":"10.1097/PAI.0000000000000627","DOIUrl":"https://doi.org/10.1097/PAI.0000000000000627","url":null,"abstract":"Special AT-rich sequence-binding protein 2 (SATB2) is an accurate marker for conventional colorectal carcinoma (CRC), although its sensitivity and specificity in mucinous tumors from the colon and other sites remains unknown. The objective of this study is to evaluate the accuracy of SATB2 expression detected by immunohistochemical assay, as a marker of primary CRC in mucinous adenocarcinomas. SATB2 immunohistochemical stains were performed on whole sections from 63 conventional CRCs (controls), 47 mucinous CRCs (mCRC), and 182 noncolorectal mucinous tumors. SATB2 intensity was scored as 1 to 3 based on the estrogen receptor/progesterone receptor grading system, and the percent positive cells was scored in broad categories as follows: 0 (negative)≤5%, 1=5% to 49%, 2≥50%. An optimal sensitivity/specificity pairing (83% and 95%, respectively) was achieved in the mCRCs when the additive intensity and percent score was ≥3 (ie, intensity score+percent score=total score). Defining this total score (histologic score/\"H score\") as a \"positive\" result, the sensitivity of SATB2 for conventional CRC was 98% (62/63) versus 83% (39/47) for mCRCs (P=0.02); whereas 5% (9/182) of all noncolorectal mucinous tumors were considered positive. SATB2 especially demonstrated reduced specificity when applied to mucinous gastroesophageal and breast carcinomas, which showed significant expression in 27% and 9% of cases, respectively. In summary, SATB2 is a less sensitive marker of colorectal origin in mCRC compared with conventional CRC and shows significantly reduced specificity in mucinous gastroesophageal and breast primaries.","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"378-385"},"PeriodicalIF":1.6,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PAI.0000000000000627","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35683255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5