Extracellular vesicles and circulating nucleic acids最新文献_第3页

Rapid in situ mutation detection in extracellular vesicle DNA. 细胞外囊泡DNA快速原位突变检测。

Extracellular vesicles and circulating nucleic acids Pub Date : 2025-02-17 eCollection Date: 2025-01-01 DOI: 10.20517/evcna.2024.69

Md Mofizur Rahman, Lixue Wang, Yundi Chen, Md Motiar Rahman, M Oli Al Islam, Luke P Lee, Yuan Wan

{"title":"Rapid in situ mutation detection in extracellular vesicle DNA.","authors":"Md Mofizur Rahman, Lixue Wang, Yundi Chen, Md Motiar Rahman, M Oli Al Islam, Luke P Lee, Yuan Wan","doi":"10.20517/evcna.2024.69","DOIUrl":"https://doi.org/10.20517/evcna.2024.69","url":null,"abstract":"Aim: A PCR- and sequencing-free mutation detection assay facilitates cancer diagnosis and reduces over-reliance on specialized equipment. This benefit was highlighted during the pandemic when high demand for viral nucleic acid testing often sidelined mutation analysis. This shift led to substantial challenges for patients on targeted therapy in tracking mutations. Here, we report a 30-min DNA mutation detection technique using Cas12a-loaded liposomes in a microplate reader, a fundamental laboratory tool. Methods: CRISPR-Cas12a complex and fluorescence-quenching (FQ) probes are introduced into tumor-derived extracellular vesicles (EV) through membrane fusion. When CRISPR-RNA hybridizes with the DNA target, activated Cas12a can trans-cleave FQ probes, resulting in fluorescence signals for the quantification of DNA mutation. Results: This method enables the detection of EGFR L858R mutation in EV DNA within 30 min. Laborious extraction, purification, and other preparation steps for EV DNA are eliminated. The need for advanced data processing is also dispensed with. In a cohort study involving 10 healthy donors and 30 patients with advanced non-small cell lung cancer (NSCLC), the assay achieved a sensitivity of 86.7%, a specificity of 90%, and an accuracy of 87.5%. Conclusion: The limit of detection of our Cas12 assay was ~ 8 × 105 EVs, corresponding to a mutation allele frequency (MAF) of ~ 10%. The MAF in late-stage cancers varies widely but often falls within 5%-50%. Therefore, without amplification of targets, this Cas12 assay can detect mutations in patients with advanced lung cancer. Future advancements in multiplex and high-throughput mutation detection using this assay will streamline self-diagnosis and treatment monitoring at home.","PeriodicalId":520322,"journal":{"name":"Extracellular vesicles and circulating nucleic acids","volume":"6 1","pages":"72-86"},"PeriodicalIF":0.0,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11977346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144065467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicles and miRNA-based therapies in triple-negative breast cancer: advances and clinical perspectives. 细胞外囊泡和基于mirna的治疗三阴性乳腺癌：进展和临床前景

Extracellular vesicles and circulating nucleic acids Pub Date : 2025-02-14 eCollection Date: 2025-01-01 DOI: 10.20517/evcna.2024.85

Caroline Patini de Rezende, Débora de Lima Alves, Luiz Gustavo de Almeida Chuffa, Debora Aparecida Pires de Campos Zuccari

{"title":"Extracellular vesicles and miRNA-based therapies in triple-negative breast cancer: advances and clinical perspectives.","authors":"Caroline Patini de Rezende, Débora de Lima Alves, Luiz Gustavo de Almeida Chuffa, Debora Aparecida Pires de Campos Zuccari","doi":"10.20517/evcna.2024.85","DOIUrl":"https://doi.org/10.20517/evcna.2024.85","url":null,"abstract":"Triple-negative breast cancer (TNBC) is one of the most aggressive and challenging subtypes for treatment, due to the lack of hormone receptors and the human epidermal growth factor receptor 2 (HER2) protein. The identification of new molecular targets is important for the development of targeted and specific therapies for TNBC patients. MicroRNAs (miRNAs) have emerged as promising molecular targets, being involved in cellular processes such as cell survival, apoptosis, differentiation, carcinogenesis, and metastasis. Extracellular vesicles (EVs) have gained prominence in areas such as drug delivery, immune modulation, biomarkers for diagnosis and prognosis, and therapeutics, due to their use as vehicles for the delivery of miRNAs, regulation of gene expression, and development of combined therapeutic strategies. In particular, mesenchymal stem cell-derived EVs (MSC-derived EVs) can transfer proteins, mRNAs/miRNAs, or DNA molecules and are being considered safer treatment options due to their inability to directly form tumors and contain lower amounts of membrane proteins such as MHC molecules. Numerous studies have highlighted the role of miRNAs in EVs in TNBC tumorigenesis, with a focus on diagnosis, prognosis, treatment selection, and monitoring. However, the development of therapies with EVs, especially MSC-derived EVs, is still in its infancy. Therefore, the aim of this review is to address new therapeutic strategies based on the delivery of miRNAs through EVs, with a focus on MSC-derived EVs, for the treatment of TNBC as an innovative therapy in oncology.","PeriodicalId":520322,"journal":{"name":"Extracellular vesicles and circulating nucleic acids","volume":"6 1","pages":"54-71"},"PeriodicalIF":0.0,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11977377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disease-specific signatures of circulating extracellular vesicles detected by the surface plasmon resonance imaging: a pilot study. 通过表面等离子体共振成像检测循环细胞外囊泡的疾病特异性特征：一项初步研究。

Extracellular vesicles and circulating nucleic acids Pub Date : 2025-02-11 eCollection Date: 2025-01-01 DOI: 10.20517/evcna.2024.82

Tatsuki Shibuta, Yukichi Takada, Shiori Nishinosono, Seiko Yasuda, Yasuhiro Ono, Yoshitaka Hirooka, Daisuke Irikura, Kensuke Saito, Tsukuru Umemura

{"title":"Disease-specific signatures of circulating extracellular vesicles detected by the surface plasmon resonance imaging: a pilot study.","authors":"Tatsuki Shibuta, Yukichi Takada, Shiori Nishinosono, Seiko Yasuda, Yasuhiro Ono, Yoshitaka Hirooka, Daisuke Irikura, Kensuke Saito, Tsukuru Umemura","doi":"10.20517/evcna.2024.82","DOIUrl":"https://doi.org/10.20517/evcna.2024.82","url":null,"abstract":"Aim: Cells in the human body release extracellular vesicles (EVs) into fluids, such as plasma, urine, and cerebrospinal fluid. EVs express tetraspanin family proteins (e.g., CD63, CD9, and CD81) and cell-specific antigens on their surface as common and specific markers, respectively. In this study, we hypothesized that the profile of blood cell-derived circulating EVs could reveal both common and specific pathophysiology in atherogenic diseases. Methods: Using surface plasmon resonance imaging (SPRi), we analyzed EVs surface molecules and identified circulating EVs in healthy controls (n = 18), patients with type 2 diabetes mellitus (T2DM; n = 71), and those with hypertension (HT; n = 47). Results: Patients with T2DM and HT exhibited distinct EV profiles: (i) CD9, CD110, CD20, activin receptor type-2A (AcvRIIA), Duffy antigen receptor for chemokine, and CD44 positive EVs were upregulated in T2DM; (ii) CD9, Maackia amurensis agglutinin lectin binding molecules (MBM), CD20, AcvRIIA, and CD44 positive EVs were upregulated in HT. By analyzing an appropriate set of three antigens or using dimensional reduction clustering, we were able to clearly differentiate between T2DM, HT, and control groups. In some patients, disease severity correlated with CD44 and CD20 in T2DM and MBM and AcvRIIA in HT. Conclusion: Our findings demonstrate that profiling of circulating EVs via the SPRi method offers a novel approach for diagnosing and monitoring human diseases.","PeriodicalId":520322,"journal":{"name":"Extracellular vesicles and circulating nucleic acids","volume":"6 1","pages":"36-53"},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11977349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acknowledgment to reviewers of Extracellular Vesicles and Circulating Nucleic Acids in 2024. 向2024年《细胞外囊泡与循环核酸》的审稿人致谢。

Extracellular vesicles and circulating nucleic acids Pub Date : 2025-02-08 eCollection Date: 2025-01-01 DOI: 10.20517/evcna.2025.05

Evcna Editorial Office

引用次数: 0

A report on the global conference on research and application of Chinese herbal medicine-derived extracellular vesicle-like particles (2024): utilizing medicinal plant-derived extracellular vesicles to advance global pharmaceutical development. 全球中草药源性细胞外囊泡样颗粒研究与应用大会（2024）报告：利用药用植物源性细胞外囊泡推动全球药物发展

Extracellular vesicles and circulating nucleic acids Pub Date : 2025-01-18 eCollection Date: 2025-01-01 DOI: 10.20517/evcna.2024.98

Yingqi Cao, Zhengting Wu, Huijing Li, Jiawen Shen, Dongxiao Li, Tianxin Qiu, Zimei Chen, Qing Zhao, Kewei Zhao

引用次数: 0

Consistency in bacterial extracellular vesicle production: key to their application in human health. 细菌细胞外囊泡生产的一致性：它们在人类健康中应用的关键。

Extracellular vesicles and circulating nucleic acids Pub Date : 2025-01-17 eCollection Date: 2025-01-01 DOI: 10.20517/evcna.2024.76

Ke Dai, Bo Liao, Xiaotian Huang, Qiong Liu

{"title":"Consistency in bacterial extracellular vesicle production: key to their application in human health.","authors":"Ke Dai, Bo Liao, Xiaotian Huang, Qiong Liu","doi":"10.20517/evcna.2024.76","DOIUrl":"https://doi.org/10.20517/evcna.2024.76","url":null,"abstract":"Bacterial extracellular vesicles (BEVs) are naturally occurring functional structures that play critical roles in bacterial life processes. These vesicles, commonly known as outer membrane vesicles (OMVs), were first found to be released by Gram-negative bacteria; however, it has since been confirmed that Gram-positive bacteria also secrete BEVs. As research advances, BEVs are increasingly utilized in diverse applications, including vaccine development and drug delivery. Nevertheless, the effective employment of BEVs in these contexts requires the acquisition of vesicles with consistent properties and functions through appropriate culture, isolation, and purification methods. This review examines the advantages and disadvantages of various purification techniques alongside the heterogeneity they may introduce. We utilize the heterogeneity of BEVs as a framework to critically analyze the barriers to their application and the factors influencing their characteristics. Additionally, we constructively propose solutions to enhance the consistency of BEVs, thereby facilitating their further development and application.","PeriodicalId":520322,"journal":{"name":"Extracellular vesicles and circulating nucleic acids","volume":"6 1","pages":"1-20"},"PeriodicalIF":0.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11977363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of cancer cell-released extracellular vesicles: have we become closer to cancer pain treatment? 癌细胞释放的细胞外囊泡的作用：我们离癌症疼痛治疗更近了吗？

Extracellular vesicles and circulating nucleic acids Pub Date : 2024-12-26 eCollection Date: 2024-01-01 DOI: 10.20517/evcna.2024.89

Iryna A Khasabova, Sergey G Khasabov, Donald A Simone

{"title":"The role of cancer cell-released extracellular vesicles: have we become closer to cancer pain treatment?","authors":"Iryna A Khasabova, Sergey G Khasabov, Donald A Simone","doi":"10.20517/evcna.2024.89","DOIUrl":"10.20517/evcna.2024.89","url":null,"abstract":"The effective management of cancer pain continues to be a challenge because of our limited understanding of cancer pain mechanisms and, in particular, how cancer cells interact with neurons to produce pain. In a study published in Pain, Inyang et al. used a mouse model of human papillomavirus (HPV1)-induced oropharyngeal squamous cell carcinoma to show a role for cancer cell-derived extracellular vesicles (cancer sEVs) in cancer pain. They found that inhibiting the release of sEVs reduced spontaneous and evoked pain behaviors, and that pain produced by sEVs is due to activation of TRPV1 channels. An innovative approach was the use of publicly available human RNA-sequencing data from unstimulated cultured human dorsal root ganglia (DRG) that were exposed to human head and neck squamous cell carcinoma (HNSCC)-derived sEVs to identify signaling pathways involved in the nascent translation associated with nociception. These studies further our understanding of functional interactions between cancer cells and neurons, and suggest an approach to identify novel targets for the treatment of cancer pain.","PeriodicalId":520322,"journal":{"name":"Extracellular vesicles and circulating nucleic acids","volume":"5 4","pages":"685-687"},"PeriodicalIF":0.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142985729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive summary of the ASEV-CzeSEV joint meeting on extracellular vesicles. ASEV-CzeSEV细胞外囊泡联合会议综述

Extracellular vesicles and circulating nucleic acids Pub Date : 2024-12-21 eCollection Date: 2024-01-01 DOI: 10.20517/evcna.2024.86

Kristyna Turkova, Jan Balvan, Gabriela Ambrozova, Andrea Galisova, Martina Hyzdalova, Carla Tripisciano, Viktor Cerny, Irma Schabussova, Wolfgang Holnthoner, Vendula Pospichalova

{"title":"A comprehensive summary of the ASEV-CzeSEV joint meeting on extracellular vesicles.","authors":"Kristyna Turkova, Jan Balvan, Gabriela Ambrozova, Andrea Galisova, Martina Hyzdalova, Carla Tripisciano, Viktor Cerny, Irma Schabussova, Wolfgang Holnthoner, Vendula Pospichalova","doi":"10.20517/evcna.2024.86","DOIUrl":"10.20517/evcna.2024.86","url":null,"abstract":"This report summarizes the ASEV-CzeSEV Joint Meeting on Extracellular Vesicles (EVs), held at the Medical University of Vienna in September 2024. The conference focused on introducing and expanding EV research and infrastructure within the Czech Republic and Austria, highlighting areas for collaboration. Key sessions featured research on EV-based diagnostics, tissue regeneration, interspecies communication and therapeutic applications, with an emphasis on shared resources and cross-border partnerships. The program included oral and poster presentations on EV engineering, new isolation techniques, and potential clinical applications, as well as industry updates on the latest EV technologies. The meeting concluded with awards for outstanding presentations reflecting the quality of work presented. Following the conference, a dedicated workshop was held on flow cytometry analysis of EVs, allowing participants to deepen their technical expertise in EV characterization. This report captures the main discussions, findings, and collaborative opportunities explored at the ASEV-CzeSEV meeting, signaling strong regional support for advancing EV research.","PeriodicalId":520322,"journal":{"name":"Extracellular vesicles and circulating nucleic acids","volume":"5 4","pages":"665-684"},"PeriodicalIF":0.0,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142985405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MOVE - how to foster European mobility for early career scientists in EV research. 移动-如何促进欧洲电动汽车研究的早期职业科学家的机动性。

Extracellular vesicles and circulating nucleic acids Pub Date : 2024-12-12 eCollection Date: 2024-01-01 DOI: 10.20517/evcna.2024.93

Michael W Pfaffl

引用次数: 0

Extracellular vesicles in liquid biopsies: there is hope for oral squamous cell carcinoma. 液体活检中的细胞外囊泡：口腔鳞状细胞癌有希望。

Extracellular vesicles and circulating nucleic acids Pub Date : 2024-12-07 eCollection Date: 2024-01-01 DOI: 10.20517/evcna.2024.29

Leanne Lee Leung, Xinyu Qu, Bojie Chen, Jason Yk Chan

{"title":"Extracellular vesicles in liquid biopsies: there is hope for oral squamous cell carcinoma.","authors":"Leanne Lee Leung, Xinyu Qu, Bojie Chen, Jason Yk Chan","doi":"10.20517/evcna.2024.29","DOIUrl":"10.20517/evcna.2024.29","url":null,"abstract":"Current approaches to oral cancer diagnosis primarily involve physical examination, tissue biopsy, and advanced computer-aided imaging techniques. However, despite these advances, patient survival rates have not significantly improved. Hence, there is a critical need to develop minimally invasive tools with high sensitivity and specificity to improve patient survival and quality of life. Liquid biopsy is a non-invasive, real-time method for predicting cancer status and potentially serves as a biomarker source for treatment response. Liquid biopsy includes rich biologically relevant components, such as circulating tumor cells, circulating tumor DNA, and extracellular vesicles (EVs). EVs are particularly intriguing due to their relatively high abundance in most biofluids, with the potential to identify specific cargo derived from circulating tumor EVs. Moreover, normal cells in lymph nodes can uptake EVs, fostering a pre-metastatic microenvironment that facilitates lymph node metastases - a common occurrence in oral cancers. This review encompasses English language publications over the last twenty years, focusing on methods for isolating EVs from saliva, blood, and lymphatic fluids, as well as the collection methods employed. Seventeen cases met the inclusion criteria according to ISEV guidelines, including 10 saliva cases, 6 blood cases, and 1 lymphatic fluid case. This review also highlighted research gaps in oral squamous cell carcinoma (OSCC) EVs, including a lack of multi-omics studies and the exploration of potential EV markers for drug resistance, as well as a notable underutilization of microfluidic technologies to translate liquid biopsy EV findings into clinical applications.","PeriodicalId":520322,"journal":{"name":"Extracellular vesicles and circulating nucleic acids","volume":"5 4","pages":"639-659"},"PeriodicalIF":0.0,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142985593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0